著者 大島 俊市|
発行日 1959-11
出版物タイトル 農学研究
47巻
1-2号
資料タイプ 紀要論文
著者 山内 己酉| 塩見 正保| 岡本 康博|
発行日 1959-11
出版物タイトル 農学研究
47巻
1-2号
資料タイプ 紀要論文
著者 木村 劼二|
発行日 1959-11
出版物タイトル 農学研究
47巻
1-2号
資料タイプ 紀要論文
著者 石井 博|
発行日 1959-11
出版物タイトル 農学研究
47巻
1-2号
資料タイプ 紀要論文
著者 平田 幸治|
発行日 1959-11
出版物タイトル 農学研究
47巻
1-2号
資料タイプ 紀要論文
著者 岡山大学農業生物研究所史編さん委員会|
発行日 1992-03-31
出版物タイトル 岡山大学農業生物研究所史
資料タイプ 図書
タイトル(別表記) Mutation of Fungicide Tolerance in Fusarium spp.
フルテキストURL 005_001_047_060.pdf
著者 呉 基日| 武田 和義|
抄録 Strains of Fusarium, causal fungi of scab disease of cereal crops, were examined for the mutation of topsin tolerance. The mutation rate of topsin tolerant spores was 10-5~10-6 and was not affected by the irradiation of ultraviolet rays nor application of topsin to the culture medium. Highly tolerant strains were easily selected on the screening medium containing topsin. Finally topsin was not effective to the selected tolerant mutants. The topsin tolerance was parallel to "benrate" tolerance. The tolerance was transmitted through hyphae, conidium and ascospore. The growth rate of hyphae of the tolerant mutants was lower than that of the original strains, but the conidia formation and the virulence of the mutants were comparable to the original strains. Since the heavy application of fungicides may increase the fungicide-tolerant mutants, crop varieties resistant to scab disease must be developed.
キーワード Fusarium spp. Fungicide tolerance Mutation.
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 47
終了ページ 60
ISSN 0916-930X
言語 日本語
論文のバージョン publisher
タイトル(別表記) Phenol Reaction of Kernels and Chromosome Location of Phenol Reaction Genes in the Genus Triticum
フルテキストURL 005_001_061_068.pdf
著者 張 成林| 武田 真| 武田 和義|
抄録 A total of 3,606 accessions of the genus Triticum involving diploid, tetraploid, hexaploid, and synthetic wheat and 7 Aegilops materials were tested for the phenol reaction in kernels. All hexaploid wheat showed a positive reaction to phenol, but deffered in staining degree. One of the synthetic wheat lines showed a negative reaction to phenol. Using monosomics, ditelosomics and nulli-tetrasomics in the common wheat cv Chinese Spring (Triticum aestivum L.), and Langdon (Triticum turgidum var. durum) disomic substitutions, genes controlling phenol reaction of kernels were located on chromosomes 2A, 2B and 2D. Synteny of the chromosome region involving the phenol reaction gene in some gramineous plants was discussed.
キーワード Phenol reaction Triticum Chromosome
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 61
終了ページ 68
ISSN 0916-930X
言語 日本語
論文のバージョン publisher
タイトル(別表記) ラン科植物に発生するシンビジウムモザイクウイルスの血清学的検出
フルテキストURL 005__001_039_046.pdf
著者 イ ワヤン ガラ| 近藤 秀樹| 前田 孚憲|
抄録 Dot-immunobinding assay (DIA) on nitrocellulose membranes and rapid immunofilter paper assay (RIPA) were examind for their usefulness in the detection of cymbidium mosaic virus (CyMV) in orchids. The minimum detection levels of CyMV by these methods were 100 ng/ml in purified preparations and at 10-4 dilution of extracts from infected leaves of orchids could be detected by these methods. Although DIA took 5 to 6 hours for the detection of the virus, it was reliable method for diagnosis of a large-number of samples. On the other hand, RIPA, which enabled detection of CyMV within a few minutes with sensitivity similar to that of DIA, will be suitable as a rapid and handy tool for virus disease diagnosis in orchids. Moreover, by RIPA, we could detect CyMV and odontoglossum ringspot virus (ORSV) simultaneously form doubly infected plant.
キーワード Serological detection Cymbidium mosaic virus Orchid
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 39
終了ページ 46
ISSN 0916-930X
言語 英語
論文のバージョン publisher
NAID 120002313874
タイトル(別表記) QTL Mapping for Powdery Mildew (Erysiphe graminis DC. f. sp. hordei EM Marchal) Resistance in Barley (Hordeum vulgare L.)
フルテキストURL 005_001_069_078.pdf
著者 岩佐 友彦| 部田 英雄| 武田 和義|
抄録 Powdery mildew, caused by Erysiphe graminis DC. f. sp. hordei EM Marchal, is a serious disease of Barley (Hordeum vulgare L.). In this study, we used molecular markers to identify the chromosomal locations carrying genes for powdery mildew resistance and to estimate the effect of each gene. Doubled haploid lines derived from Steptoe×Morex (S/M), Harrington×TR306 (H/T) and their parental were inoculated with five powdery mildew strains. Several quantitative trait loci (QTL) controlling E. graminis resistance were found and lacated on chromosome 4H, 5H, 6H and 7H in S/M. On the other hand, no QTL was detected in H/T but Harrington had a major resistant gene (Mlg) for powdery mildew resistance. Maker-assisted selection was conducted to examine the effect of accumulation for mildew resistance. There was a significant interaction between QTLs lacated in 4H and 7H, which suggested the presence of an epistatic effect between these QTLs.
キーワード Hordeum vulgare Erysiphe graminis QTL mapping DH lines
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 69
終了ページ 78
ISSN 0916-930X
言語 日本語
論文のバージョン publisher
タイトル(別表記) 日本におけるCymbidium属植物から分離されて生物学的性質の異なるOdontoglossum Ringspot Virus分離株のペプチドマッピングによる比較
フルテキストURL 005_001_031_038.pdf
著者 近藤 秀樹| 前田 孚憲| 井上 成信|
抄録 Symptoms on Cymbidium, double-stranded (ds) RNA pattern and peptide mapping of coat protein (CP) of five isolates of odontoglossum ringspot virus from Cymbidium in Japan were compared. One of the isolates, Cy-1, that produced unique symptoms on Cymbidium, showed a distinct peptide mapping pattern from those of the other four isolates by partial digestion of CP with pepsin. All the isolates produced three major dsRNA species of Mr=4.3,1.4 and 0.6×106 in the infected plants.
キーワード Odontoglossum ringspot virus Cymbidium Peptide mapping Double-stranded RNA
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 31
終了ページ 38
ISSN 0916-930X
言語 英語
論文のバージョン publisher
NAID 120002313419
タイトル(別表記) 2種のモノクローナル抗体を用いた簡易ELISAによるキュウリモザイクウイルス迅速・高感度検出
フルテキストURL 005_001_023_030.pdf
著者 前田 孚憲| 佐古 宣道| 井上 成信|
抄録 To detect cucumber mosaic virus (CMV), virus samples and conjugate were incubated together in a simplified double-antibody sandwich ELISA. The use of the same monoclonal antibody (MAb) as trapping (coating) and detecting antibodies resulted in considerable decrease of ELISA values and sensitivity due to the competition for antigen between trapping and detecting antibodies. The simplified ELISA using two MAbs which recognize different epitopes of CMV proved to be a rapid and sensitive method for virus detection.
キーワード Cucumber mosaic virus Monoclonal antibody Simplified ELISA
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 23
終了ページ 30
ISSN 0916-930X
言語 英語
論文のバージョン publisher
タイトル(別表記) ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出
フルテキストURL 005_001_079_096.pdf
著者 齊藤 美奈子| 木口 忠彦| 玉田 哲男|
抄録 Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV.
キーワード Sugar beet Rhizomania BNYVV RNA detection Nonradioactive cDNA probe
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 79
終了ページ 96
ISSN 0916-930X
言語 英語
論文のバージョン publisher
タイトル(別表記) Measurements of Response of Barley and Wheat to Enviromental Factors with an Open System Porometer
フルテキストURL 005_001_011_021.pdf
著者 米谷 俊彦| 柏木 良明|
抄録 The rates of photosynthesis and transpiration were measured in barley and wheat under various environmental conditions, with an open system porometer. The rates of photosynthesis and transpiration in the horizontal leaf and vertical leaf had different diurnal variations. The rate of photosynthesis in the vertical leaf was highest in the morning and in the afternoon, while that in the horizontal leaf was highest before noon. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v SARI and Akanmugi) of barley grown in submerged soil conditions. At the end of April, chlorophyll contents had decreased and the maintenance respiration acquired in spite of continuous transpiration. The rapid change of photosynthetically active radiation did not affect the rates of photosynthesis or stomatal conductance of SARI grown in submerged soil. The rates of photosynthesis and transpiration and chlorophyll contents were measured for two species(c.v. Hongmangmai and Haruhikari) of wheat grown under different soil water conditions. Chlorophyll content tended to increase in dry soil conditions. Hongmangmai had a higher chlorophyll content than Haruhikari, even at the beginning of May. Hongmangmai had large photosynthetic rate and small transpiration rates under dry soil conditions. These confirm that Hongmangmai has a prominent drought stress tolerance. The open system porometer and the chlorophyll meter may be very useful for comparing physiological characteristics of the plant's response to environmental factors and clarifying differences between plant species.
キーワード Barley Hongmangmai Photosynthesis rate Chlorophyll content Submerged soil Dry soil
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 11
終了ページ 21
ISSN 0916-930X
言語 日本語
論文のバージョン publisher
タイトル(別表記) ライムギ小型染色体を保持する普通系コムギからのライムギ型cDNAのディファレンシャルスクリーニング
フルテキストURL 006_001_053_064.pdf
著者 村田 稔|
抄録 Occurrence of the midget chromosome in a common wheat with rye cytoplasm [(cereale)-Chinese Spring (CS)] indicates that the chromosome carries the essential gene(s) for maintaining the function of rye cytoplasm. To elucidate the interaction between the midget chromosome and rye cytoplasm, in this study, an attempt was made to isolate rye-type cDNAs from a cDNA library of (cereale)-CS by differential screening. Two replica filters from each plate were hybridized with digoxigenin (DIG)-labeled wheat CS cDNAs and with DIG-labeled rye cDNAs,respectively. Out of ca. 20,000 plaques, 27 were hybridized more strongly with rye cDNAs than with CS cDNAs. These clones were classified into six classes (Ⅰ-Ⅵ) by blot hybridization. The majority of the clones (21 out of 27) was belonged to the same class (1), showing rye-type RFLP (restriction fragment length polymorphism). The DNA sequence of clone CrClA in class Ⅰ, was very similar to that of wheat ribulose 1,5-bisphosphate carboxylase,large subnit gene, rbcL(94.5% homology). However, the 3' end of CrClA was shorter than that of wheat rbcL, and terminated at TAA instead of TAG, like the rbcL of Aegilops crassa. In the clone CrC5.4, the first half of the sequence was similar to that of one rice EST clone, the functions of which are not known, and the latter was similar to the reverse sequence of maize 4.5S-23S ribosomal RNA. This suggests that CrC5.4 had been derived from two defferent cDNAs of (cereale)-CS. Three other clones had homology to the chlorophyll a/b binding protein genes (cab) of wheat, maize and tomato, and one to wheat rbcS (ribulose1,5-bisphosphate carboxylase small subnit gene). However, no clear polymorphisms were detected between wheat and rye by using those clones as probes.
キーワード Cytoplasm substitution line Differential screening Midget chromosome Rye Wheat
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1999
6巻
1号
開始ページ 53
終了ページ 64
ISSN 0916-930X
言語 英語
論文のバージョン publisher
タイトル(別表記) ホウレンソウ種子に存在するα-グルコシダーゼの分子多型変化
フルテキストURL 005_001_001_009.pdf
著者 古井 聡| 杉本 学| 鈴木 幸雄|
抄録 Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70 kDa by gel filtration, respectively. α-Glucosidase A had high activity not only toward maltooligosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 65℃ for 20 min. On the other hand, α-glucosidase B had high activity toward maltooligosaccharides but faint activity toward α-glucans. The optimum pH was 5.0 and no activity was found after incubation at 65℃ for 20 min. The enzymatic and immunological properties of α-glucosidase A and B were similar to those of α-glucosidase. Ⅰor Ⅱ, and α-glucosidase Ⅲ or Ⅳ, isolated from spinach seeds without 4℃ storage, respectively. These findings suggest that the α-glucosidase in spinach seeds is modified to be two molecular forms.
キーワード Spinach α-Glucosidase Multiple molecular forms
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1997
5巻
1号
開始ページ 1
終了ページ 9
ISSN 0916-930X
言語 英語
論文のバージョン publisher
タイトル(別表記) エビネから分離されたインゲンマメ黄斑モザイクウイルスの諸性質
フルテキストURL 006_001_043_051.pdf
著者 松本 純一| 前田 孚憲| 井上 成信|
抄録 Bean yellow mosaic virus (BYMV) was isolated from Calanthe sp.showing mild chlorosis on the leaves, collected in Yamaguchi Prefecture in Japan. The virus was transmitted by the aphid Myzus persicae in a non-persistent manner, and by sap-inoculation to 29 out of 46 plant species from 9 out of 12 families tested. Stability in crude sap, morphology of virus particles, shape of cylidrical inclusions and the presence of cytoplasmic crystalline inclusions in the infected cells were similar to those of BYMV isolates previously reported. The virus contained a single protein species with a molecular weight of 35,000. In a microprecipition test and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the virus showed a close serological relationship to isolates of BYMV from both crocus and gladiolus, and showed a distant relationship to clover yellow vein virus. Three BYMV isolates used in this study were found to be serologically related to each other, but the virus was more closely related to the BYMV crocus isolate than to gladiolus isolate.
キーワード Calanthe sp. bean yellow mosaic virus clover yellow vein virus serological relationship
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1999
6巻
1号
開始ページ 43
終了ページ 51
ISSN 0916-930X
言語 英語
論文のバージョン publisher
タイトル(別表記) Varietal Variation and Mechanism of Hull-cracked Grains in Two-rowed Barley
フルテキストURL 004_001_089_096.pdf
著者 金谷 良一| 武田 和義|
抄録 Hull-cracked grain which causes low germinability and low malt extract has been observed in malting barley varieties mainly in the western part of Japan. In the first part of this study, more than 600 two-rowed barley varieties were examined for the percentage of hull-cracked grains. A markedly skewed frequency distribution pattern was observed for varietal variation of hull-cracked grain percentage. More than 80% of the varieties developed less than 5% hull-cracked grains, while a few of the varieties frequently developed the hull-cracked grains. The maximum hull-cracked grain percentage was as high as 61% in Yoshikei 16. Improved varieties developed hull-cracked grains more frequently than the local varieties, indicating varietal improvement indirectly caused the hull-cracked grains. In the second part, nine two-rowed varieties were grown in eight different conditions to analyze the mechanism of hull-cracking. Variances due to varieties, environment and their interaction were all statistically significant. Some of the varieties developed almost no hull-cracked grains throughout the environmental conditions examined, while others sharply responded to the environmental conditions. The environmental correlation coefficient between 1,000-kernel weight and hull-cracked grains was as high as 0.918, indicating that hull-cracked grains had developed under favorable ripening conditions.
キーワード Barley Varietal varietal Hull-crached grain Ripening
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1996
4巻
1号
開始ページ 89
終了ページ 96
ISSN 0916-930X
言語 日本語
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タイトル(別表記) The Characteristics of Trace Metal Distribution of Artificial and Natural-Origin Suspended Particulate Matter (SPM) by grain size
フルテキストURL 006_001_029_041.pdf
著者 村本 茂樹| 米谷 俊彦| 平岡 直子| 青山 勲|
抄録 Air pollution caused by suspended particulate matter (SPM) has increased remarkedly in industrial areas, and this has become an emergency issue in the global environment. Atomospheric SPM influences the local ecosystem including various plants and animals. It is necessary to examine the distribution characteristics of such trace metals in fine particulates (especially those with a diameter (φ) of less than 2.1μm). The Environment Agency of the Japanese goverment will complete analysis of the a concentration of suspended particulate matter less than 2.4μm by the end of 1999, and will be examining the relationship between the concentration of suspended particulate matter (0.1-9.0μm) with consideration given to the particle size throughout two years. It became clear that the metals of artificial origin such as Pb, V, Cd, Ni, Cr, existed in fine particulates (0.1 or more 2.0μm). By contrast, the metals of natural origin, such as Ti, Mn, Mg, Sr, mainly existed in coarse Area in Japan.
キーワード Atomospheric Suspended particulate matter (SPM) Natural-origin metal Artificial-origin metal Biological effects
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1999
6巻
1号
開始ページ 29
終了ページ 41
ISSN 0916-930X
言語 日本語
論文のバージョン publisher
タイトル(別表記) QTL Mapping for Water Sensitivity in Barley Seeds
フルテキストURL 006_001_021_028.pdf
著者 岩佐 友彦| 高橋 秀和| 武田 和義|
抄録 Sensitivity to water condition during germination is an important character in malting barley because it affects malt poduction. Water sensitivity in malting barley is defined as the defference in germination percentage between the seeds in standard and excess-water conditions. Usually standard and excess-water condition means 4 and 8 ml water in a 90 mm perti dish, respectively. Takeda and Fukuyama (1983) examined more than 4,000 barley accessions and found 0~100% water sensitivity. This means complete to no suppression of germination by an excess-water condition. In this study, quantiative trait loci (QTL) analysis was applied to analize the inheritance of water sensitivity. Two sets of doubled haploid (DH) populations derived from Steptoe×Morex (S/M) and Harrington×TR306 (H/T) crosses,were grown in the experiment field of the Research Institute for Bioresources, Okayama University. Bulbosum method was adapted to develop DH lines (Chen and Hayes 1989). Water sensitivities of 150 (S/M) and (H/T) lines and their parents were evaluated using 50 seeds each and the germination test (25℃, 4 days) was repeated four times. Water sensitivity showed a large variation (Fig.1) and the heritability of the trait was 0.78 and 0.72 in S/M and H/T, respectively, indicating that about three-quarters of the phenotypic variance was genetic in origin. Three (S/M) and one (H/T) significant QTL were detected and 23% (S/M) and 25% (H/T) of the phenotypic variance was explained by the QTL. These QTL were located on chromosomes 2H,3H,6H and 7H (Table 1, Fig. 2). QTL relating to germination traits were lacated near the QTL controlling water sensitivity (Fig. 3). Interaction among the QTL was small (Table 2). Marker-assisted selection was effective (Table 3).
キーワード barley water sensitivity QTL analysis
出版物タイトル 岡山大学資源生物科学研究所報告
発行日 1999
6巻
1号
開始ページ 21
終了ページ 28
ISSN 0916-930X
言語 日本語
論文のバージョン publisher