ラット脾臓の動脈終止,灌流固定試料の走査電子顕微鏡観察

Rat spleens were perfused arterially with physiological saline and glutaraldehyde, freeze-cracked in liquid nitrogen, conductively stained with tannin-osmium-thiocarbohydrazide-osmium, critical-point-dried with liquid carbon dioxide, and observed under a scanning electron microscope. The terminals of the follicular arteries ended in the interstitial tissue spaces between the marginal zone and white pulp, and those of the penicillar arteries ended in the interstitial tissue spaces of the Billroth cords. Only a faw connections of the arterial terminals with the venous sinuses were noted. These findings strongly support the "open theory" that in the mammalian spleen the arterial blood mainly flows into the tissue spaces before being received by the venous sinuses.