Elsevier Acta Medica Okayama 2405-8440 10 11 2024 Bacterial DNA and serum IgG antibody titer assays for assessing infection of human-pathogenic and dog-pathogenic Porphyromonas species in dogs e31872 EN Masako Tai-Tokuzen Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital Takashi Ito Center for Innovative Clinical Medicine, Okayama University Hospital Kazuya Tamura Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Haruko Hirayama Department of Animal Resources, Advanced Science Research Center, Okayama University Hirohito Ogawa Department of Virology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Shin Nakamura Center for Collaborative Research, Department of Oral Science and Translational Research, Nova Southeastern University Keisuke Okubo Department of Periodontics and Endodontics, Division of Dentistry, Okayama University Hospital Kazuhiro Omori Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Tadashi Yamamoto Department of Comprehensive Dentistry, The Center for Graduate Medical Education (Dental Division), Okayama University Hospital Katsumi Mominoki Department of Animal Resources, Advanced Science Research Center, Okayama University Shogo Takashiba Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Periodontal disease is highly prevalent in both humans and dogs. Although there have been reports of cross-infection of periodontopathic bacteria, methods for assessing it have yet to be established. The actual status of cross-infection remains to be seen. The purpose of this study was to evaluate the utility of bacterial DNA and serum immunoglobulin G (IgG) antibody titer assays to assess infection of human-pathogenic and dog-pathogenic Porphyromonas species in dogs. Four experimental beagles were used for establishing methods. Sixty-six companion dogs at veterinary clinics visiting for treatment and prophylaxis of periodontal disease were used and divided into healthy, gingivitis, and periodontitis groups. Periodontal pathogens such as Porphyromonas gingivalis and Porphyromonas gulae were investigated as target bacteria. DNA levels of both bacteria were measured using species-specific primers designed for real-time polymerase chain reaction (PCR). Serum IgG titers of both bacteria were measured by enzyme-linked immunosorbent assay (ELISA).<br> PCR primers were confirmed to have high sensitivity and specificity. However, there was no relationship between the amount of bacterial DNA and the severity of the periodontal disease. In addition, dogs with periodontitis had higher IgG titers against both bacteria compared to dogs in the healthy and gingivitis groups; there was cross-reactivity between the two bacteria. Receiver operating characteristic (ROC) analysis of IgG titers against both bacteria showed high sensitivity (>90 %) and specificity (>75 %). Since both bacteria were distinguished by DNA assays, the combination of these assays may be useful in the evaluation of cross-infection. No potential conflict of interest relevant to this article was reported.

Public Library Science Acta Medica Okayama 1932-6203 17 9 2022 Prevention of non-infectious pulmonary complications after intra-bone marrow stem cell transplantation in mice e0273749 EN Yoshiko Yamasuji-Maeda Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hisakazu Nishimori Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Keisuke Seike Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Akira Yamamoto Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hideaki Fujiwara Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Taiga Kuroi Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kyosuke Saeki Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Haruko Fujinaga Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Sachiyo Okamoto Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Ken-Ichi Matsuoka Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Nobuharu Fujii Department of Transfusion Medicine, Okayama University Hospital Takehiro Tanaka Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Masahiro Fujii Department of Animal Resources, Advanced Science Research Center, Okayama University Katsumi Mominoki Department of Animal Resources, Advanced Science Research Center, Okayama University Takuro Kanekura Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences Yoshinobu Maeda Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Non-infectious pulmonary complications including idiopathic pneumonia syndrome (IPS) and bronchiolitis obliterans syndrome (BOS), which are clinical and diagnostic manifestations of lung chronic graft-versus-host disease (GVHD), cause significant mortality after allogeneic stem cell transplantation (SCT). Increasing evidence suggests that alloantigen reactions in lung tissue play a central role in the pathogenesis of IPS and BOS; however, the mechanism is not fully understood. Several clinical and experimental studies have reported that intrabone marrow (IBM)-SCT provides high rates of engraftment and is associated with a low incidence of acute GVHD. In the present study, allogeneic SCT was conducted in mouse models of IPS and BOS, to compare intravenous (IV)-SCT with IBM-SCT. Allogeneic IBM-SCT improved the clinical and pathological outcomes of pulmonary complications compared to those of IV-SCT. The mechanisms underlying the reductions in pulmonary complications in IBM-SCT mice were explored. The infiltrating lung cells were mainly CD11b+ myeloid and CD3+ T cells, in the same proportions as in transplanted donor cells. In an in vivo bioluminescence imaging, a higher proportion of injected donor cells was detected in the lung during the early phase (1 h after IV-SCT) than after IBM-SCT (16.7 +/- 1.1 vs. 3.1 +/- 0.7 x 10(5) photons/s/animal, IV-SCT vs. IBM-SCT, P = 1.90 x 10(-10)). In the late phase (5 days) after SCT, there were also significantly more donor cells in the lung after IV-SCT than after IBM-SCT or allogeneic-SCT (508.5 +/- 66.1 vs. 160.1 +/- 61.9 x 10(6) photons/s/animal, IV-SCT vs. IBM-SCT, P = 0.001), suggesting that the allogeneic reaction induces sustained donor cell infiltration in the lung during the late phase. These results demonstrated that IBM-SCT is capable of reducing injected donor cells in the lung; IBM-SCT decreases donor cell infiltration. IBM-SCT therefore represents a promising transplantation strategy for reducing pulmonary complications, by suppressing the first step in the pathophysiology of chronic GVHD. No potential conflict of interest relevant to this article was reported.

The Japanese Society of Veterinary Science Acta Medica Okayama 0916-7250 81 8 2019 Risk assessment for hepatitis E virus infection from domestic pigs introduced into an experimental animal facility in a medical school 1191 1196 EN Hirohito Ogawa Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Haruko Hirayama Department of Animal Resources, Advanced Science Research Center, Okayama University Satsuki Tanaka Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Norio Yata Department of Animal Resources, Advanced Science Research Center, Okayama University Hikaru Namba Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Nobuko Yamashita Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kenzo Yonemitsu Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University Ken Maeda Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University Katsumi Mominoki Department of Animal Resources, Advanced Science Research Center, Okayama University Masao Yamada Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Hepatitis E virus (HEV) is known to cause zoonotic infections from pigs, wild boars and deer. Domestic pigs have been used as an experimental animal model in medical research and training; however, the risks of HEV infection from pigs during animal experiments are largely unknown. Here, we retrospectively investigated the seroprevalence and detection rates of viral RNA in 73 domestic pigs (average 34.5 kg) introduced into an animal experimental facility in a medical school during 2012-2016. We detected anti-HEV immunoglobulin G antibodies in 24 of 73 plasma samples (32.9%), though none of the samples were positive for viral RNA. Plasma samples of 18 pigs were sequentially monitored and were classified into four patterns: sustained positive (5 pigs), sustained negative (5 pigs), conversion to positive (6 pigs) and conversion to negative (2 pigs). HEV genomes were detected in 2 of 4 liver samples from pigs that were transported from the same farm during 2016-2017. Two viral sequences of the overlapping open reading frame (ORF) 2/3 region (97 bp) were identical and phylogenetically fell into genotype 3. A 459-bp length of the ORF2 region of an amplified fragment from a pig transported in 2017 was clustered with the wbJYG1 isolate (subgenotype 3b) with 91.5% (420/459 bp) nucleotide identity. Based on our results, we suggest that domestic pigs introduced into animal facilities carry a potential risk of HEV infection to researchers, trainees and facility staff. Continuous surveillance and precautions are important to prevent HEV infection in animal facilities. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 35 2019 会長あいさつ 1 1 EN Katsumi Mominoki Department of Animal Resources, Advanced Science Research Center, Okayama University No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 32 2016 e-learning システムを用いた動物実験教育訓練知識確認テスト 33 36 EN Norio Yata Chika Uefuji Haruko Hirayama Katsumi Mominoki The Animal Care and Use Committee, Okayama University have conducted the lecture, which is provided to researchers and students, who use the mammalian, avian and/or reptilian species for research. To use these species for research and education in our university, researchers and students must need to attend this lecture, pass the examination on the e-learning system, “the Okayama University WebClass”.This system is useful in order to improve the effectiveness of education and training. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 32 2016 岡山大学における動物実験に関わる機関管理体制の構築 ―非医療系キャンパスでの機関管理拠点として動物実験施設の整備コンセプトとその運用― 5 8 EN Katsumi Mominoki It has been important for academic research institutions to properly use the laboratory animals for research and education, pursuant to the provisions of the Law, Care and Management Standards, and the Fundamental Guidelines concerning animal experiments and related activities. However, in the several faculties, which do not have the centralized animal facilities, it appears that using the animals are inconsistent with these ordinances. To avoid that the inconsistency becomes any problems, Okayama University has been established new centralized animal facility to propel the institutional management. This content presents our concept of establishing the facility to properly conduct researchers and students in the above-mentioned faculties. No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 27 2011 岡山大学自然生命科学研究支援センター動物資源部門津島北施設 52 55 EN Katsumi Mominoki Rumi Takashima Akihiro Matsukawa No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 25 2009 岡山大学自然生命科学研究支援センター動物資源部門鹿田施設 65 68 EN Katsurni Mominoki Kazuhiko Ochiai No potential conflict of interest relevant to this article was reported.

岡山実験動物研究会 Acta Medica Okayama 18 2001 愛媛大学医学部附属動物実験施設 48 51 EN Takashi Fujiwara Katsumi Mominoki No potential conflict of interest relevant to this article was reported.