岡山大学資源生物科学研究所 Acta Medica Okayama 0916-930X 5 2 1998 Characteristics of Pectic Polysaccharides from Rice Shoots 135 144 EN Haruyoshi Konno Hisaaki Tsumuki Yoshiki Yamasaki Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4〜0.6% of the starch-free cell walls. No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0916-930X 5 2 1998 サトイモのα-グルコシダーゼの精製と性質 129 134 EN Hideyuki Mashima Yoshiki Yamasaki Haruyoshi Konno α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0916-930X 5 2 1998 Purification and Properties of α-Glucosidase from Slugs 121 127 EN Yoshiki Yamasaki Haruyoshi Konno Three forms of α-glucosidase(EC3.2.1.20), designated as �T, �U,�V,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase �V　hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase �T hyrolyzed soluble starch more slowly. No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0916-930X 1 2 1993 Purification and Properties of Wall-bound α-Glucosidase from Suspension-cultured Sugar-beet Cells 159 166 EN Yoshiki Yamasaki Haruyoshi Konno Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. No potential conflict of interest relevant to this article was reported.

岡山大学資源生物科学研究所 Acta Medica Okayama 0916-930X 1 2 1993 Extracellular Polysaccharides from the Culture Medium of Cell Suspension Cultures of Carrot 91 103 EN Haruyoshi Konno Yoshiki Yamasaki Kenji Katoh The extracellular polysaccharides have been fractionated from the culture medium of carrot(Daucus carota)cell culrures by precipitation with ethanol and by chromatography on DEAE-Sepharose CL-6B DEAE-Trisacryl M ion-exchange and Bio-Gel A-1.5m gel-permeation.The sugar composition and molecular mass of purified neutral and acidic polymers were determined. The neutral and acidic polymers were treated with purified endo-Β-glucanase from Trichoderma viride and pectic depolymerases,such as endo-pectate lyase from Erwinia carotovora Er. and endo-polygalacturonase from Kluyveromyces fragilis, respectively. The "hairly"(ramified)regions of acidic polymer were sequentially treated with purified α-L-arabinofuranosidase and β-galactosidase from carrot cell cultures, and were further hydrolyzed with 50mM trifluoroacetic for 1 hr at 100℃. From these results, the extracellular polysaccharides secreted from carrot cell cultures are charactarized. No potential conflict of interest relevant to this article was reported.