| タイトル（別表記） | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
|---|---|
| フルテキストURL | 004_002_164_174.pdf |
| 著者 | Gara I Wayan| 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| キーワード | Vanda orchid Cymbidium mosaic virus Potexvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 164 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313836 |
| タイトル（別表記） | Detection of the Viruses Occurring in Oriental Cymbidium in Japan |
|---|---|
| フルテキストURL | 004_002_149_162.pdf |
| 著者 | 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A survey of virus diseases occurring in Oriental Cymbidium collected from a commerical nursery and home garden in Japan was conducted in 1991-1994. Identification of the vurus was based on partcle morphology, symptomatology in indicator plants, ultrastructure of infected cells and serology. Four viruses, odontoglossum ringspot tabamovirus(ORSV), cymbidium mosaic potexvirus(CyMV), orchid fleck virus (ORV) and a previously underscribed spherical virus, were found in 27 out of 37 Cymbidium plants tested. ORSV was detected from 11 plants belinging to Cym. ensifolium, Cym. forrestii, Cym. goeringii, Cym. kanran, Cym. sinense and Cymbidium spp. showing chlorotic streaks and/or mild mosaic. CyMV was isolated from only one plant of Cymbidium sp. showing mosaic and necrotic spots on leaves. In negatibvely stained dip preparations from plants infected with ORSV and CyMV, rod shaped particles of ca. 310 nm and flexuous rod-shaped ca. 475 nm in length were observed, respectively. The viruses were reacted strongly with respective antiserum to each virus in immunosorbent electron microcopy and inderect ELISA. OFV was isolated from four plants of Cym. formosanum, Cym. kanran, Cym. sinense and Cymbidium sp. showing mosaic and necrotic flecks. The virus had non-enveloped, bullet-shaped particles about 40×120～150 nm in dip preparation. The undescribed spherical virus, ca. 28 nm diameter, was isolated from 11 plants of Cym. forrestii, Cym. goeringii and Cymbidium spp. showing stunting and chlorotic streaks on newly developed leaves. The virus was mechanically transmitted only to Cymbidium orchids. Previously, we designated it as cymbidium chlorotic mosaic sobemovirus(CyCMV)(Kondo et al,1994),as the virus was considered to be a new member of the genus Sobemovirsu. |
| キーワード | Oriental Cymbidium Odontoglossum ringspot tobamovirus Cymbidium mosaic potexvirus Orchid fleck virus Cymbidium chlorotic mosaic sobemovirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 149 |
| 終了ページ | 162 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313470 |
| タイトル（別表記） | Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe |
|---|---|
| フルテキストURL | 004_002_119_135.pdf |
| 著者 | 井上 成信| 松本 純一| 前田 孚憲| 光畑 興二| 近藤 秀樹| 田原 望武| |
| 抄録 | Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV･So from Cymbidium. |
| キーワード | Calanthe Orchid fleck virus Calanthe yellowish fleck disease |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 119 |
| 終了ページ | 135 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002314037 |
| タイトル（別表記） | Host Range and Some Properties of Orchid Fleck Virus Isolated form Oriental Cymbidium in Japan |
|---|---|
| フルテキストURL | 003_002_151_161.pdf |
| 著者 | 近藤 秀樹| 松本 純一| 前田 孚憲| 井上 成信| |
| 抄録 | Orchid flck virus (OFV) was isolated from Oriental Cymbidium (Cymbidium sp.),showing chlorotic flecks on leaves. The virus was transmitted mechanically to Chenopodium quinoa,C.murale and Beta vulgaris by sap-inoculation and caused systemic infection. Local lesions were produced on C.amaranticolar, Petunia hybrida, Tetragonia expansa and Vigna sinensis. Sap from infected T.expansa was still infective after 10 mim at 40℃ but not after 10 min at 45℃, at a dilution of 10-3 but not 10-4, and after 30 min at room temperature but not after 60 min. The isolate of OFV had non-enveloped, bullet-shaped patricles measuring about 40×120－150 nm in dip preparations. However, bacilliform particles about 40×120-140 nm were observed in ultrathin sections. In ultrahtin sections of virus-infected tissues, virus patricles were detected both in the nuclei and in the cytoplasm. Moreover, the inclusions of low electron density(viroplasm) were also observed in the nuclei.Virus particles were found to attach at one end to the inner nuclear membrane. A number of particles surrounded by the inner membrane often showed an appearance like a spoked wheel. |
| キーワード | Orchid fleck virus Oriental Cymbidium Non-enveloped rhabdovirus like particles |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 2号 |
| 開始ページ | 151 |
| 終了ページ | 161 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313940 |
| タイトル（別表記） | Host Range and Properties of Odontoglossum Ringspot Tobamovirus (ORSV) Isolated from Miniature Cymbidium in Japan |
|---|---|
| フルテキストURL | 001_001_021_034.pdf |
| 著者 | 近藤 秀樹| 前田 孚憲| 井上 成信| |
| 抄録 | Odontoglossum ringspot virus (ORSV)はJensen＆Gold（1951）によってOdontoglossum grandeにえそ輪紋を生じた病原ウイルスに命名されたが、CattleyaやCymbidiumを中心として広くラン科植物に発生していることが認められている。また、わが国では井上（1966、1983）がCymbidiumからORSVを分離し報告しており、日本でも多種属のラン科植物にORSVの発生が非常に多いことが認められている。シュンラン、カンラン、スルガランなどを含む東洋ラン・Cymbidium属では、かつて葉にモザイク斑を生じた斑紋を”金砂”と呼び非常に珍重されていたが、井上（1984、1990）はこの斑紋がORSVの感染によって生じたものであることを記載した。さらに、井上・呂（1983）は台湾においてもORSVが東洋ランに発生していることを報告している。これらの報告にはウイルスの診断と病徴の記載にとどまり、その病原ウイルスの諸性質についての詳細な記述がなされていなかった。本報告では、葉に軽いモザイク症状を示す東洋ランと呼ばれているスルガラン（Cymbidium ensifolium：蕙蘭）およびカンラン（Cymb. kanran）から検出されたウイルスについて病原学的研究を行ったところ、ORSVと同定されたので、これらの諸性質についての研究結果を報告するとともに、この東洋ラン分離株と既報の洋ランから分離されているORSVおよび同グループのtobacco mosaic virus （TMV）との比較研究を行ったので、それらの結果についても報告する。 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 21 |
| 終了ページ | 34 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313489 |
| フルテキストURL | O004150.pdf |
|---|---|
| 著者 | 近藤 秀樹| |
| 発行日 | 2006-09-30 |
| 資料タイプ | 学位論文 |
| 学位授与番号 | 乙第4150号 |
| 学位授与年月日 | 2006-09-30 |
| 学位・専攻分野 | 博士（農学） |
| 授与大学 | 岡山大学 |
| 言語 | 日本語 |