Okayama University Medical School Acta Medica Okayama 0386-300X 55 3 2001 Nephrin is an important component of the barrier system in the testis. 161 165 EN Li Liu Kunihiko Aya Hiroyuki Tanaka Junya Shimizu Shigeru Ito Yoshiki Seino Article /10.18926/AMO/32022 <p>Nephrin, a gene product of the congenital nephrotic syndrome of the Finnish type (NPHS1), is a 1242-residue putative transmembrane protein of the immunoglobulin family of cell adhesion molecules. The expression of this gene is localized in rat and human glomerular epithelial cells. Here we report the expression of nephrin in various tissues other than the kidneys in mice. The expression of nephrin mRNA in various tissues of mice, including the kidneys, testes, spleen, thymus and brain, were first investigated by the RT-PCR method, and it was shown that a high level of nephrin mRNA could be detected in the testes of mice 1-6 weeks old. In situ hybridization revealed the expression of the nephrin gene in the Sertoli cells. Additionally, immunofluorescent staining studies indicated that nephrin was colocalized with anchoring protein ZO-1 in the mouse testis. From these results, it is inferred that nephrin is an important component of the barrier system in testes.</p> No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 109 7-12 1997 腎臓における副甲状腺ホルモン関連ペプチドの局在の検討 133 140 EN Kunihiko Aya PTHrP mRNA was quantified by RT-PCR and the localization of PTHrP mRNA was determined by in situ hybridization in kidney. During development, the expression of PTHrP mRNA is much higher before 2 weeks of age while kidney is developing. In E16 fetal mice, PTHrP mRNA was strongly expressed in the collecting ducts, urothelium of the pelvis, tubles, the glomeruli and immature elements in the cortex of the developing kidney (nephrogenic zone). within 0-day-old-mice, it was dominantly expressed in collecting ducts, the urothelium of the pelvis and nephrogenic zone. These findings suggest that PTHrP plays a role in kidney development as an autocrine/ paracrine factor. No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 1997 腎臓における副甲状腺ホルモン関連ペプチドの局在の検討 EN No potential conflict of interest relevant to this article was reported.