Pergamon-Elsevier Science Ltd. Acta Medica Okayama 0003-9969 59 12 2014 Assessment of intraoral mucosal pain induced by the application of capsaicin 1334 1341 EN Ryuji Terayama Kotaro Maruhama Hiroki Tsuchiya Masahide Mizutani Seiji Iida Tomosada Sugimoto Objective To develop an objective method for assessing nociceptive behaviour in an animal model of capsaicin-induced intraoral pain. Changes in nociceptive responses were also examined after injury to the inferior alveolar nerve (IAN). Design Nociceptive responses evoked by the intraoral application of various doses of capsaicin were analyzed in lightly anaesthetized rats. The number of c-Fos protein-like immunoreactive (Fos-LI) neurons in the medullary dorsal horn (MDH) induced by the intraoral application of capsaicin was measured. Behavioural and c-Fos responses were also examined 14 days after injury to the IAN. Results Larger doses of intraoral capsaicin (1, 10 and 100 ƒÊg) induced vigorous licking behaviour and c-Fos response in the MDH in a reproducible manner. The magnitudes of both behavioural activity and the c-Fos response from the 10 and 100 ƒÊg doses of capsaicin were significantly greater than that by the 1 ƒÊg dose. Injury to the IAN exaggerated the behavioural and c-Fos responses evoked by intraoral capsaicin. Conclusions The intraoral application of capsaicin is a valid and reliable method for studying intraoral pain and hyperalgesia following nerve injury. No potential conflict of interest relevant to this article was reported.