松下治

Clinical and microbiological characteristics of high-level daptomycin-resistant Corynebacterium species: A systematic scoping review

著者	Fukushima, Shinnosuke\| Hagiya, Hideharu\| Gotoh, Kazuyoshi\| Tsuji, Shuma\| Iio, Koji\| Akazawa, Hidemasa\| Matsushita, Osamu\| Otsuka, Fumio\|
キーワード	Antimicrobial resistance Corynebacterium Daptomycin High-level daptomycin resistance pgsA2
備考	© 2024 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/\| This fulltext file will be available in Jan. 2026.\|
発行日	2024-12-03
出版物タイトル	Journal of Infection and Chemotherapy
巻	31巻
号	2号
出版者	Elsevier
開始ページ	102575
ISSN	1341-321X
NCID	AA11057978
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2024 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control.
論文のバージョン	author
PubMed ID	39638285
DOI	10.1016/j.jiac.2024.12.004
Web of Science KeyUT	001416617600001
関連URL	isVersionOf https://doi.org/10.1016/j.jiac.2024.12.004

Examination of yield, bacteriolytic activity and cold storage of linker deletion mutants based on endolysin S6_ORF93 derived from Staphylococcus giant bacteriophage S6

フルテキストURL	fulltext.pdf
著者	Munetomo, Sosuke\| Uchiyama, Jumpei\| Takemura-Uchiyama, Iyo\| Wanganuttara, Thamonwan\| Yamamoto, Yumiko\| Tsukui, Toshihiro\| Hagiya, Hideharu\| Kanamaru, Shuji\| Kanda, Hideyuki\| Matsushita, Osamu\|
発行日	2024-10-23
出版物タイトル	PLoS ONE
巻	19巻
号	10号
出版者	Public Library of Science
開始ページ	e0310962
ISSN	1932-6203
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2024 Munetomo et al.
論文のバージョン	publisher
PubMed ID	39441843
DOI	10.1371/journal.pone.0310962
Web of Science KeyUT	001345720700068
関連URL	isVersionOf https://doi.org/10.1371/journal.pone.0310962

Phenotypic and Genetic Characteristics of Carbapenemase-Producing Enterobacterales Isolates at Okayama University Hospital

JaLCDOI	10.18926/AMO/67657
フルテキストURL	78_5_371.pdf
著者	Gotoh, Kazuyoshi\| Miyoshi, Makoto\| I Putu Bayu Mayura\| Tsuji, Shuma\| Iio, Koji\| Fukushima, Shinnosuke\| Matsushita, Osamu\| Hagiya, Hideharu\|
抄録	Spread of carbapenemase-producing Enterobacterales (CPE) is an ongoing public health issue worldwide, including in Japan. In this study, we investigated the phenotypic and genetic characteristics of CPE isolates at Okayama University Hospital over the 5 years (2013-2018) prior to the outbreak of the 2019 coronavirus pandemic. Of 24 carbapenem-resistant Enterobacterales isolated during the study period, we identified 8 CPE isolates harboring blaIMP-1 (5 isolates) and blaIMP-6 genes (3 isolates). Bacterial species and carbapenem susceptibility patterns exhibited diversity. Minimum inhibitory concentrations (MICs) of meropenem were generally higher than those of imipenem and biapenem. Results of pulsed-field gel electrophoresis demonstrated that neither clonal nor plasmid-mediated outbreaks of blaIMP-harboring CPE isolates have developed at our hospital. One Klebsiella oxytoca isolate showed a high MIC (128 μg/mL) of meropenem, which could be explained by the high plasmid copy number. Subsequent analysis of this isolate may elucidate the intricacies of carbapenem resistance profiles among CPE isolates. Collectively, our findings underscore the necessity for ongoing genetic surveillance of CPE, complemented by tailored approaches for infection prevention and control.
キーワード	antimicrobial resistance carbapenemase-producing enterobacterales carbapenemase-resistant enterobacterales Silent pandemic whole genome sequence
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2024-10
巻	78巻
号	5号
出版者	Okayama University Medical School
開始ページ	371
終了ページ	376
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	Copyright Ⓒ 2024 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	39467655
Web of Science KeyUT	001343346400003

Cefazolin inoculum effect in methicillin-susceptible Staphylococcus aureus clinical isolates

著者	Tsuji, Shuma\| Gotoh, Kazuyoshi\| Manabe, Tadahiro\| Iio, Koji\| Fukushima, Shinnosuke\| Matsushita, Osamu\| Hagiya, Hideharu\|
キーワード	Beta-lactamase blaZ Cefazolin Inoculum effect Staphylococcus aureus
備考	© 2024 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/\| This fulltext file will be available in Jun. 2025.\|
発行日	2024-09
出版物タイトル	Diagnostic Microbiology and Infectious Disease
巻	110巻
号	1号
出版者	Elsevier BV
開始ページ	116399
ISSN	0732-8893
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2024 Elsevier Inc.
論文のバージョン	author
PubMed ID	38875894
DOI	10.1016/j.diagmicrobio.2024.116399
Web of Science KeyUT	001255589200001
関連URL	isVersionOf https://doi.org/10.1016/j.diagmicrobio.2024.116399

Metataxonomic Analysis of the Uterine Microbiota Associated with Low Fertility in Dairy Cows Using Endometrial Tissues Prior to First Artificial Insemination

フルテキストURL	fulltext.pdf
著者	Yagisawa, Takuya\| Uchiyama, Jumpei\| Takemura-Uchiyama, Iyo\| Ando, Shun\| Ichii, Osamu\| Murakami, Hironobu\| Matsushita, Osamu\| Katagiri, Seiji\|
キーワード	dairy cows low fertility uterine microbiota microbial diversity bacterial association
発行日	2023-04-26
出版物タイトル	Microbiology Spectrum
巻	11巻
号	3号
出版者	American Society for Microbiology
開始ページ	e04764-22
ISSN	2165-0497
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2023 Yagisawa et al.
論文のバージョン	publisher
PubMed ID	37098918
DOI	10.1128/spectrum.04764-22
Web of Science KeyUT	000975042800001
関連URL	isVersionOf https://doi.org/10.1128/spectrum.04764-22

Prevalence of Inducible Macrolide, Lincosamide, and Streptogramin B (inducible MLSB) Resistance in Clindamycin-Susceptible Staphylococcus aureus at Okayama University Hospital

JaLCDOI	10.18926/AMO/64355
フルテキストURL	77_1_1.pdf
著者	Nahar, Lutfun\| Hagiya, Hideharu\| Nada, Takahiro\| Iio, Koji\| Gotoh, Kazuyoshi\| Matsushita, Osamu\| Otsuka, Fumio\|
抄録	Inducible resistance to the macrolide, lincosamide, and streptogramin B (iMLSB) antibiotic family is a latent mechanism for antimicrobial resistance in Staphylococcus aureus. We here investigated the frequency and genotypic profiles of iMLSB resistance in clindamycin (CLDM)-susceptible S. aureus isolated in Okayama University Hospital from June 2020 to June 2021. We phenotypically screened the iMLSB resistance via D-zone test and performed PCR testing for the erythromycin ribosomal methylase (erm) genes: ermA and ermC. Among 432 CLDM-susceptible S. aureus isolates, 138 (31.9%) exhibited an iMLSB-resistance phenotype, with methicillinresistant S. aureus isolates (MRSA; 61 isolates: 58.6%) exhibiting higher positivity than methicillin-sensitive S. aureus isolates (MSSA; 77 isolates: 23.5%) (p<0.001). Male patients had a higher frequency of iMLSB resistance than females (OR [95%CI]: 1.8 [1.2-2.8]; p=0.007). Genotypically, ermA predominated in both MSSA (70.1%) and MRSA (86.9%) compared to ermC (14.3% in MSSA and 11.5% in MRSA). A single strain of MRSA possessed both ermA and ermC, while 12 (15.6%) MSSA isolates were negative for both ermA and ermC, suggesting the presence of other genetic mechanisms. Collectively, these results show that approximately 33% of CLDM-susceptible S. aureus isolates at our university hospital exhibited iMLSB resistance, predominantly caused by ermA in both MSSA and MRSA.
キーワード	antimicrobial resistance clindamycin erm D-zone test inducible MLSB
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2023-02
巻	77巻
号	1号
出版者	Okayama University Medical School
開始ページ	1
終了ページ	9
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	Copyright Ⓒ 2023 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	36849140
Web of Science KeyUT	000953663800001

Effects of Helicobacter pylori and Nitrate-Reducing Bacteria Coculture on Cells

フルテキストURL	fulltext.pdf
著者	Ojima, Hinako\| Kuraoka, Sakiko\| Okanoue, Shyoutarou\| Okada, Hiroyuki\| Gotoh, Kazuyoshi\| Matsushita, Osamu\| Watanabe, Akari\| Yokota, Kenji\|
キーワード	Helicobacter pylori nitrate-reducing bacteria IL-8 TNF-alpha cell cycle
発行日	2022-12-16
出版物タイトル	Microorganisms
巻	10巻
号	12号
出版者	MDPI
開始ページ	2495
ISSN	2076-2607
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2022 by the authors.
論文のバージョン	publisher
PubMed ID	36557748
DOI	10.3390/microorganisms10122495
Web of Science KeyUT	000903731600001
関連URL	isVersionOf https://doi.org/10.3390/microorganisms10122495

Detection of Enterobacter cloacae complex strain with a blaNDM-1-harboring plasmid from an elderly resident at a long-term care facility in Okayama, Japan

フルテキストURL	fulltext20221116-2.pdf
著者	Gotoh, Kazuyoshi\| Hagiya, Hideharu\| Iio, Koji\| Yamada, Haruto\| Matsushita, Osamu\| Otsuka, Fumio\|
キーワード	Antimicrobial resistance Carbapenemase-producing Enterobacterales Carbapenem-resistant Enterobacterales New Delhi metallo-β-lactamase (NDM) Enterobacter cloacae complex
備考	© 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.jiac.2022.08.019] .\| This full-text will be available in Oct. 2023.\|
発行日	2022-12
出版物タイトル	Journal of Infection and Chemotherapy
巻	28巻
号	12号
出版者	Elsevier BV
開始ページ	1697
終了ページ	1699
ISSN	1341-321X
NCID	AA11057978
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2022 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases.
論文のバージョン	author
PubMed ID	36049614
DOI	10.1016/j.jiac.2022.08.019
Web of Science KeyUT	000874559900019
関連URL	isVersionOf https://doi.org/10.1016/j.jiac.2022.08.019

Phylogenic analysis of new viral cluster of large phages with unusual DNA genomes containing uracil in place of thymine in gene-sharing network, using phages S6 and PBS1 and relevant uncultured phages derived from sewage metagenomics

フルテキストURL	fulltext20220830-2.pdf table20220830-2pdf.pdf Figs20220830-2.pdf Supplementary_data.pdf
著者	Uchiyama, Jumpei\| Takemura-Uchiyama, Iyo\| Gotoh, Kazuyoshi\| Kato, Shin-ichiro\| Sakaguchi, Yoshihiko\| Murakami, Hironobu\| Fukuyama, Tomoki\| Kaneki, Mao\| Matsushita, Osamu\| Matsuzaki, Shigenobu\|
キーワード	Environmental virus Jumbophage Metagenomics Evolution Uncultured phage
備考	© 2022 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.virusres.2022.198881] . \|
発行日	2022-10-02
出版物タイトル	Virus Research
巻	319巻
出版者	Elsevier BV
開始ページ	198881
ISSN	0168-1702
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2022 Elsevier B.V.
論文のバージョン	author
PubMed ID	35934259
DOI	10.1016/j.virusres.2022.198881
Web of Science KeyUT	000841172200001
関連URL	isVersionOf https://doi.org/10.1016/j.virusres.2022.198881

In vitro effectiveness of biapenem against IMP-producing Enterobacteriaceae

フルテキストURL	fulltext20211206-1.pdf
著者	Gotoh, Kazuyoshi\| Miyoshi, Makoto\| Mayura, I Putu Bayu\| Iio, Koji\| Matsushita, Osamu\| Otsuka, Fumio\| Hagiya, Hideharu\|
備考	This is an Accepted Manuscript published by Microbiology Society.\|
発行日	2021-10-4
出版物タイトル	Journal of Medical Microbiology
巻	70巻
号	10号
出版者	Microbiology Society
ISSN	0022-2615
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2021 Microbiology Society
論文のバージョン	author
PubMed ID	34605760
DOI	10.1099/jmm.0.001430
Web of Science KeyUT	000718022100014
関連URL	isVersionOf https://doi.org/10.1099/jmm.0.001430

Detection of in-frame mutation by IS30-family insertion sequence in the phospholipid phosphatidylglycerol synthase gene (pgsA2) of high-level daptomycin-resistant Corynebacterium striatum

フルテキストURL	fulltext20211109-5.pdf
著者	Gotoh, Kazuyoshi\| Mayura, I. Putu Bayu\| Enomoto, Yusaku\| Iio, Koji\| Matsushita, Osamu\| Otsuka, Fumio\| Hagiya, Hideharu\|
キーワード	Antimicrobial Resistance Daptomycin resistance Corynebacterium striatum Insertion sequence pgsA2 gene
備考	This is an Accepted Manuscript of an article published by Springer. This fulltext is available in Oct. 2022.\|
発行日	2021-10-21
出版物タイトル	European Journal of Clinical Microbiology & Infectious Diseases
巻	41巻
号	2号
出版者	Springer Science and Business Media LLC
開始ページ	331
終了ページ	333
ISSN	0934-9723
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature
論文のバージョン	author
PubMed ID	34671843
DOI	10.1007/s10096-021-04369-1
Web of Science KeyUT	000709253000001
関連URL	isVersionOf https://doi.org/10.1007/s10096-021-04369-1

Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection

フルテキストURL	fulltext.pdf
著者	Ichihara, Aina\| Ojima, Hinako\| Gotoh, Kazuyoshi\| Matsushita, Osamu\| Take, Susumu\| Okada, Hiroyuki\| Watanabe, Akari\| Yokota, Kenji\|
キーワード	antibody VacA CagA genome
発行日	2021-07-05
出版物タイトル	toxins
巻	13巻
号	7号
出版者	MDPI
開始ページ	467
ISSN	2072-6651
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2021 by the authors.
論文のバージョン	publisher
DOI	10.3390/toxins13070467
Web of Science KeyUT	000677052200001
関連URL	isVersionOf https://doi.org/10.3390/toxins13070467

Antibacterial Effects of Disulfiram in Helicobacter pylori

フルテキストURL	fulltext.pdf
著者	Kobatake, Tomomi\| Ogino, Keiki\| Sakae, Hiroyuki\| Gotoh, Kazuyoshi\| Watanabe, Akari\| Matsushita, Osamu\| Okada, Hiroyuki\| Yokota, Kenji\|
キーワード	disulfiram Helicobacter pylori urease vacuolating toxin CagA
発行日	2021-05-12
出版物タイトル	Infection and Drug Resistance
巻	14巻
出版者	Dove Medical Press Ltd
開始ページ	1757
終了ページ	1764
ISSN	1178-6973
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2021 Kobatake et al.
論文のバージョン	publisher
PubMed ID	34012274
NAID	120007042392
DOI	10.2147/IDR.S299177
Web of Science KeyUT	000653055700001
関連URL	isVersionOf https://doi.org/10.2147/IDR.S299177

Acceleration of bone regeneration of horizontal bone defect in rats using collagen‐binding basic fibroblast growth factor combined with collagen scaffolds

フルテキストURL	fulltext.pdf
著者	Nakamura, Shin\| Ito, Takashi\| Okamoto, Kentaro\| Mima, Takehiko\| Uchida, Kentaro\| Siddiqui, Yasir D.\| Ito, Masahiro\| Tai, Masako\| Okubo, Keisuke\| Yamashiro, Keisuke\| Omori, Kazuhiro\| Yamamoto, Tadashi\| Matsushita, Osamu\| Takashiba, Shogo\|
キーワード	bone regeneration collagen drug delivery systems growth factors periodontitis tissue engineering
発行日	2019-03-19
出版物タイトル	Journal of Periodontology
巻	90巻
号	9号
出版者	Wiley
開始ページ	1043
終了ページ	1052
ISSN	0022-3492
NCID	AA00704417
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2019 The Authors
論文のバージョン	publisher
PubMed ID	30889294
DOI	10.1002/JPER.18-0674
Web of Science KeyUT	000485288400012
関連URL	isVersionOf https://doi.org/10.1002/JPER.18-0674

The Genetic Diversity of Helicobacter pylori Virulence Genes Is Not Associated with Gastric Atrophy Progression

JaLCDOI	10.18926/AMO/49667
フルテキストURL	67_2_93.pdf
著者	Kita, Masahide\| Yokota, Kenji\| Okada, Hiroyuki\| Take, Susumu\| Takenaka, Ryuta\| Kawahara, Yoshiro\| Oguma, Keiji\| Matsushita, Osamu\| Yamamoto, Kazuhide\|
抄録	Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer and duodenal ulcer accompanied by atrophic gastritis in background mucosa. H. pylori strains were stored at －80℃ prior to the experiments being carried out. We analyzed iceA, babA, vacA, cagA, and cagE genes by PCR. The cagA gene was analyzed through sequencing of the C-terminal region containing the EPIYA motif, which is related to tyrosine phosphorylation. Severe atrophy was observed in patients with gastric ulcer. The major phenotype of the vacA gene was s1c/m1 (93ｵ). The cagA gene was detected in all strains. The cagE gene was not detected in 2 and 5 strains from the mild cases and severe cases, respectively. The major cagA EPIYA motif, which is amino acids repeat in the C terminus, was the A-B-D type (44 of 58 strains). The virulence genes were not statistically associated with the severity of atrophy in the background gastric mucosa in humans. Not only identification of bacterial virulence factors but also studies of the host response will be necessary to investigate the progression of gastric atrophy and subsequent cancer development in humans.
キーワード	Helicobacter pylori virulence genes chronic atrophic gastritis
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2013-04
巻	67巻
号	2号
出版者	Okayama University Medical School
開始ページ	93
終了ページ	98
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	23603925
Web of Science KeyUT	000317801700003
関連URL	http://ousar.lib.okayama-u.ac.jp/metadata/52508

Phospholipase C Produced by Clostridium botulinum Types C and D:Comparison of Gene, Enzymatic, and Biological Activities with Those of Clostridium perfringens Alpha-toxin

JaLCDOI	10.18926/AMO/49252
フルテキストURL	67_1_9.pdf
著者	Fatmawati, Ni Nengah Dwi\| Sakaguchi, Yoshihiko\| Suzuki, Tomonori\| Oda, Masataka\| Shimizu, Kenta\| Yamamoto, Yumiko\| Sakurai, Jun\| Matsushita, Osamu\| Oguma, Keiji\|
抄録	Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival.
キーワード	botulinum phospholipase C botulinum toxin phospholipase C activity sphingomyelinase activity hemolytic activity
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2013-02
巻	67巻
号	1号
出版者	Okayama University Medical School
開始ページ	9
終了ページ	18
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	23439504
Web of Science KeyUT	000316829900002
関連URL	http://ousar.lib.okayama-u.ac.jp/metadata/49731

松下 治