Okayama University Medical SchoolActa Medica Okayama0386-300X5642002Localization of dynamin 2 in rat seminiferous tubules during the spermatogenic cycle.205209ENHirokiIguchiMasamiWatanabeAkihiroKamitaniAtsushiNagaiOsamuHosoyaKimikoTsutsuiHiromiKumonArticle10.18926/AMO/31681<p>Dynamin is a protein essential to endocytosis. Dynamin 2, a dynamin isoform, is expressed most intensely in testicular tissue; however, precise localization has never been studied. Therefore, we investigated the expression of dynamin 2 in rat testicular tissue using immunohistochemical methods, and discuss here the physiological function of this protein. Testicular tissues were obtained from Wistar rats at 10, 21 and 63 days of age. Immunohistochemistrical examination and Western blot analysis were conducted using dynamin 2 specific antibody. Western blot analysis showed that expression in 21- and 63-day-old rats was more intense than that in 10-day-old rats. Dynamin 2 expression was observed using immunohistochemical method in the seminiferous tubules of all rats. In the 63-day-old rats, the expression was intense, especially in spermatids in the earlier maturation stages and in spermatocytes, and was observed in Sertoli cells. However, in spermatids, the expression gradually declined as spermatids matured to spermatozoa. In the 21-day-old rats, the expression was evident in spermatocytes and Sertoli cells, but that in the 10-day-old rats was weak. Intense expression of dynamin 2 during spermatogenesis suggests that this protein plays an important role in this process.</p>
No potential conflict of interest relevant to this article was reported.Okayama University Medical SchoolActa Medica Okayama0386-300X3921985Difference in the homology of two nuclear nonhistone protein fractions as compared by polyacrylamide gel electrophoresis.99104ENKenTsutsuiKimikoTsutsuiKojiAoyamaTakuzoOdaArticle10.18926/AMO/31512<p>The extent of homology between two protein fractions was compared by simple electrophoretic analysis. Nuclear proteins of several rodent cells of different origins were fractionated into acid-soluble and acid-insoluble fractions. The two protein fractions were subjected to polyacrylamide gel electrophoresis in separate gel systems, and protein bands with identical mobilities were sought either in all possible combinational pairs of cell types or in all cell types. The paired and overall homology indices calculated from these data and chi-square testing of the results indicated that acid-soluble nuclear nonhistone proteins are more homologous than acid-insoluble nuclear proteins. Several factors which might have affected the results were discussed.</p>
No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558907-81978マウス腹水肉腫細胞核からの高分子量酸可溶性蛋白質の精製とその性質9991013ENKimikoTsutsuiA high molecular weight acid soluble nuclear protein (HAN-1) was isolated in a electrophoretically homogeneous state from mouse ascites sarcoma cells (SR-C3H cells) by the exclusion chromatography in Sephacryl S-200 and the DEAE-Sephadex chromatography of the nuclear 0.2M H(2)SO(4) extract. The content of HAN-1 in SR-C3H nuclei was about 4.3% per total histone, the highest among the cell types tested. The molecular weight of HAN-1 was estimated to be 125,000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of HAN-1 was rich in glutamic acid, alanine, lysine and aspartic acid, the acidic/basic amino acid ratio 1.8. The in vitro RNA synthesizing activity of SR-C3H cell RNA polymerases Ⅰ and Ⅱ on a naked DNA template was differentially affected by HAN-1; the reaction with polymerase I was inhibited and that with polymerase Ⅱ stimulated.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155812132009新見 嘉兵衛先生を偲んで227ENKimiko M .TsutsuiNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155812132009DNAトポイソメラーゼⅡβによる神経関連遺伝子の活性化機構143147ENKuniakiSanoMaryMiyajiM. KimikoTsutsuiKenTsutsuiNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155811412002徳永叡教授と脳の研究6769ENNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155811622004Ⅱ型 DNAトポイソメラーゼと神経細胞分化103107ENNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama1978マウス腹水肉腫細胞核からの高分子量酸可溶性蛋白質の精製とその性質ENNo potential conflict of interest relevant to this article was reported.