start-ver=1.4 cd-journal=joma no-vol=130 cd-vols= no-issue=1 article-no= start-page=24 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=201601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Muscarinic acetylcholine receptor M1 and M3 subtypes mediate acetylcholine-induced endothelium-independent vasodilatation in rat mesenteric arteries en-subtitle= kn-subtitle= en-abstract= kn-abstract= The present study investigated pharmacological characterizations of muscarinic acetylcholine receptor (AChR) subtypes involving ACh-induced endothelium-independent vasodilatation in rat mesenteric arteries. Changes in perfusion pressure to periarterial nerve stimulation and ACh were measured before and after the perfusion of Krebs solution containing muscarinic receptor antagonists. Distributions of muscarinic AChR subtypes in mesenteric arteries with an intact endothelium were studied using Western blotting. The expression level of M1 and M3 was significantly greater than that of M2. Endothelium removal significantly decreased expression levels of M2 and M3, but not M1. In perfused mesenteric vascular beds with intact endothelium and active tone, exogenous ACh (1, 10, and 100 nmol) produced concentration-dependent and long-lasting vasodilatations. In endothelium-denuded preparations, relaxation to ACh (1 nmol) disappeared, but ACh at 10 and 100 nmol caused long-lasting vasodilatations, which were markedly blocked by the treatment of pirenzepine (M1 antagonist) or 4-DAMP (M1 and M3 antagonist) plus hexamethonium (nicotinic AChR antagonist), but not methoctramine (M2 and M4 antagonist). These results suggest that muscarinic AChR subtypes, mainly M1, distribute throughout the rat mesenteric arteries, and that activation of M1 and/or M3 which may be located on CGRPergic nerves releases CGRP, causing an endothelium-independent vasodilatation. en-copyright= kn-copyright= en-aut-name=TangsucharitPanot en-aut-sei=Tangsucharit en-aut-mei=Panot kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakatoriShingo en-aut-sei=Takatori en-aut-mei=Shingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZamamiYoshito en-aut-sei=Zamami en-aut-mei=Yoshito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=GodaMitsuhiro en-aut-sei=Goda en-aut-mei=Mitsuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=PakdeechotePoungrat en-aut-sei=Pakdeechote en-aut-mei=Poungrat kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakayamaFusako en-aut-sei=Takayama en-aut-mei=Fusako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Clinical Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Clinical Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Department of Pharmaceutical Care and Health Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Department of Clinical Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Department of Physiology, Faculty of Medicine, Khon Kaen University kn-affil= affil-num=6 en-affil= Department of Clinical Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Department of Clinical Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=Acetylcholine-induced vasodilatation kn-keyword=Acetylcholine-induced vasodilatation en-keyword=CGRPergic nerves kn-keyword=CGRPergic nerves en-keyword=Muscarinic receptor subtypes kn-keyword=Muscarinic receptor subtypes en-keyword=Rat mesenteric arteries kn-keyword=Rat mesenteric arteries END start-ver=1.4 cd-journal=joma no-vol=128 cd-vols= no-issue=3 article-no= start-page=419 end-page=424 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080301 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of postprandial hyperglycemia and hyperinsulinemia on vascular responsiveness kn-title=食後高血糖が血管反応性に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Recent clinical studies demonstrated that transient postprandial hyperglycemia and hyperinsulinemia may contribute to the development of hypertension. Therefore, we investigated influence of acute hyperglycemia and/or hyperinsulinemia induced by glucose or insulin infusion on neuronal and humoral control of vascular tone in rats. Euglycemic male Wistar rats were pithed under anesthesia and arterial blood pressure was measured. Changes in vascular responses to spinal cord stimulation (SCS) and intravenous bolus injections of noradrenaline, angiotensin II, calcitonin generelated peptide (CGRP), acetylcholine and sodium nitroprusside (SNP) were studied by infusing various concentration of glucose or insulin. Continuous glucose infusion, which increased both blood glucose and serum insulin levels, significantly augmented adrenergic nerve-mediated pressor responses to SCS without affecting injection of pressor responses to noradrenaline or angiotensin II. In pithed rats with artificially increased blood pressure and blockade of autonomic outflow, glucose infusion attenuated CGRPergic nerve-depressor responses to SCS without affecting depressor responses to injection of CGRP, acetylcholine or SNP. In pithed rats treated with octreotide, which increased blood glucose without increasing serum insulin levels, glucose infusion caused only significant augmentation of adrenergic nervemediated pressor responses. Combined infusion of insulin and glucose, which resulted in increased serum insulin levels with euglycemic, significantly augmented adrenergic nerve-mediated pressor responses and attenuated CGRPergic nerve-mediated depressor responses. The present results suggest that acute hyperglycemia and hyperinsulinemia increases adrenergic nerve-mediated vasoconstriction, which is partly associated with the blunted CGRPergic nerve function, and that plasma insulin concentration associated with hyperglycemia may be responsible for alteration of neuronal vascular regulation. en-copyright= kn-copyright= en-aut-name=ZamamiYoshito en-aut-sei=Zamami en-aut-mei=Yoshito kn-aut-name=座間味義人 kn-aut-sei=座間味 kn-aut-mei=義人 aut-affil-num=1 ORCID= en-aut-name=TakatoriShingo en-aut-sei=Takatori en-aut-mei=Shingo kn-aut-name=高取真吾 kn-aut-sei=高取 kn-aut-mei=真吾 aut-affil-num=2 ORCID= en-aut-name=IwataniYukiko en-aut-sei=Iwatani en-aut-mei=Yukiko kn-aut-name=岩谷有希子 kn-aut-sei=岩谷 kn-aut-mei=有希子 aut-affil-num=3 ORCID= en-aut-name=YamawakiKosuke en-aut-sei=Yamawaki en-aut-mei=Kosuke kn-aut-name=山脇康佑 kn-aut-sei=山脇 kn-aut-mei=康佑 aut-affil-num=4 ORCID= en-aut-name=MiyashitaSatoko en-aut-sei=Miyashita en-aut-mei=Satoko kn-aut-name=宮下智子 kn-aut-sei=宮下 kn-aut-mei=智子 aut-affil-num=5 ORCID= en-aut-name=YabumaeNana en-aut-sei=Yabumae en-aut-mei=Nana kn-aut-name=藪前奈々 kn-aut-sei=藪前 kn-aut-mei=奈々 aut-affil-num=6 ORCID= en-aut-name=TakayamaFusako en-aut-sei=Takayama en-aut-mei=Fusako kn-aut-name=高山房子 kn-aut-sei=高山 kn-aut-mei=房子 aut-affil-num=7 ORCID= en-aut-name=MioMitsunobu en-aut-sei=Mio en-aut-mei=Mitsunobu kn-aut-name=見尾光庸 kn-aut-sei=見尾 kn-aut-mei=光庸 aut-affil-num=8 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name=川﨑博己 kn-aut-sei=川﨑 kn-aut-mei=博己 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=2 en-affil= kn-affil=日本新薬(株)創薬研究所 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 affil-num=8 en-affil= kn-affil=就実大学薬学部薬効解析学分野 affil-num=9 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科臨床薬学分野 en-keyword=hyperglycemia kn-keyword=hyperglycemia en-keyword=hyperinsulinemia kn-keyword=hyperinsulinemia en-keyword=Calcitonin gene-related peptide nerve kn-keyword=Calcitonin gene-related peptide nerve END start-ver=1.4 cd-journal=joma no-vol=63 cd-vols= no-issue=2 article-no= start-page=105 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Beneficial effects of Vitis coignetiae Pulliat leaves on nonalcoholic steatohepatitis in a rat model en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Vitis coignetiae Pulliat (Yamabudo) is used as a health juice and wine based on the abundant polyphenols and anthocyanins in its fruit. However, it is not known whether the leaves of this plant confer similar benefits. This study investigated the hepatoprotective effects of aqueous extracts from Vitis coignetiae Pulliat leaves (VCPL) in an animal model of nonalcoholic steatohepatitis (NASH). Rats were fed a choline-deficient high-fat diet for four weeks to generate fatty livers. NASH was induced by oxidative stress loading. Ten weeks later, blood and liver samples were collected from anesthetized animals and assessed biochemically, histologically, and histochemically to determine the extent of oxidative stress injury and the overall effects of VCPL. Six-week VCPL extract supplementation reduced serum levels of liver enzymes, decreased CYP2E1 induction, increased plasma antioxidant activities and delayed the progression of liver fibrosis. The findings suggested that VCPL has strong radical-scavenging activity and may be beneficial in preventing NASH progression.

en-copyright= kn-copyright= en-aut-name=TakayamaFusako en-aut-sei=Takayama en-aut-mei=Fusako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamotoKazuo en-aut-sei=Nakamoto en-aut-mei=Kazuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KawasakiHiromu en-aut-sei=Kawasaki en-aut-mei=Hiromu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MankuraMitsumasa en-aut-sei=Mankura en-aut-mei=Mitsumasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=EgashiraToru en-aut-sei=Egashira en-aut-mei=Toru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UekiKeiji en-aut-sei=Ueki en-aut-mei=Keiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HasegawaAzusa en-aut-sei=Hasegawa en-aut-mei=Azusa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OkadaShigeru en-aut-sei=Okada en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Clinical Pharmaceutical Science, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Clinical Pharmaceutical Science, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Clinical Pharmaceutical Science, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Anti-Aging Food Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Anti-Aging Food Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Hiruzen Winery Co., Ltd. affil-num=7 en-affil= kn-affil=Department of Clinical Pharmaceutical Science, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Anti-Aging Food Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Anti-Aging Food Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Yamabudo kn-keyword=Yamabudo en-keyword=nonalcoholic steatohepatitis kn-keyword=nonalcoholic steatohepatitis en-keyword=antioxidant kn-keyword=antioxidant en-keyword=hepatoprotection kn-keyword=hepatoprotection END