Faculty of Engineering, Okayama University Acta Medica Okayama 0475-0071 36 1 2001 Improvement of Biocompatibility of Silicone Elastomer by Surface Modification 117 121 EN Yuki Shirosaki Kanji Tsuru Satoshi Hayakawa Akiyoshi Osaka Seisuke Takashima 10.18926/47005 γ-Methacryloxypropyltrimethoxysilane (γ-MPS) was grafted to silicone due to emulsion polymerization to induce Si-OH groups, in order to provide silicone with bioactivity spontaneous deposition of apatite in body fluid and to improve cytocompatibility. Apatite deposited on the grafted silicone within 7 days of soaking in 1.5 times as concentrated as the Kokubo solution. Osteoblastic cells (MC3T3-E1) were cultured on the specimens up to 7 days. After 5 days of culture, the number of MC3T3-E1 cells on the grafted specimen was much greater than that on the original specimen. These results indicated that the biocompatibility of silicone elastomer was improved by the grafting γ-MPS. No potential conflict of interest relevant to this article was reported.

Faculty of Engineering, Okayama University Acta Medica Okayama 0475-0071 34 1-2 2000 Cytocompatibility of Silicone Elastomer Treated with Hydrogenperoxide Containing Tantalum Chloride 39 43 EN Satoshi Hayakawa Yuki Shirosaki Takeshi Yabuta Kanji Tsuru Akiyoshi Osaka 10.18926/15363 Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5). No potential conflict of interest relevant to this article was reported.

Acta Medica Okayama 2005 Synthesis of two- and three dimensional organic-inorganic hybrids for inducing biomineralization EN Yuki Shirosaki No potential conflict of interest relevant to this article was reported.