Okayama University Medical School Acta Medica Okayama 0386-300X 57 1 2003 Enhancement of norepinephrine-induced transient contraction in aortic smooth muscle of diabetic mice. 45 48 EN Asaki Abe Chiaki Kawasoe Yasuhiro Kondo Katsunori Sato Article 10.18926/AMO/32840 <p>Changes in norepinephrine-induced transient contractions in Ca2+-deficient solution were investigated in the aortic smooth muscles of diabetic ALS (alloxan-induced diabetes susceptible) mice. The transient contractions in diabetic mice were significantly larger than those in normal mice. The longer incubation of the muscle preparations in Ca2+-deficient solution made the transient contractions smaller, probably due to the leakage and decrease in norepinephrine-releasable stored Ca2+. The rate of this reduction in contraction was slower in diabetic mice. These results suggest that the leakage of intracellular stored Ca2+ caused by extracellular Ca2+ deficiency is attenuated in diabetic mice, contributing to enhanced norepinephrine-induced transient contractions.</p> No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254　 98 1 2009 低アレルギー発症性が言及されている鶏卵の機能特性に関する動物実験 31 37 EN Kumiko Nagato Asaki Abe Kiyoaki Narabara Yasuhiro Kondo Functional features concerned with low proallergic natures were examined using an allergy-inducible rat strain (Brown Norway rat; BN rat) on hen's eggs which have been empirically mentioned as hypoallergenic for patients suffering from food allergies (experimental eggs). BN rats were fed on feed containing whole experimental eggs (feed E) and whole normal eggs (control feed, feed C). The densities of immunoglobulin E (IgE)-positive cells, have reported to be IgE-bearing mast cells, in the jejunum and ileum of BN rats fed on experimental-egg-containing feed were lower than those of BN rats fed on normal-egg-containing feed. The number of blood eosinophils was also lower in BN rats fed on feed E. Serum IgE levels were no different between BN rats fed on feed E and feed C. These results indicate that the low proallergic nature of hen's eggs studied in the present study is due to the dereased ability of experimental eggs to facilitate the proliferation and induction of mast cells in the intestinal tissue. No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254 93 1 2004 コレステロール負荷によって発症したニワトリ胸部大動脈と冠状動脈における動脈硬化症 45 49 EN Yasuhiro Kondo Sachika Honjo Kazumasa Ohtsuki Asaki Abe Hypercholesterolemia and hyperlipidemia have been important in human health as factors that induce atherosclerotic lesions in brain and heart blood vessel. Various experimental studies have been done to prevent and treat atherosclerotic lesions in animals. In the present study, we assessed the suitability of chickens as experimental animal for atherosclerosis. Newly hatched chicks were fed on 1.0%-or 0.1%-cholesterol(CHO) containing feed. After 3 and 6 months, total cholesterol levels in the sera and histological changes in the aorta and coronary artery of chicks fed on 1.0%-CHO-containing feed for 3 and 6 months, intimal thickening and marked accumulation of foam cells were observed. Endothelial cells had disappeared in the aorta of these chicks. A slight accumulation of foam cells was observed in the aortic intima of chicks fed on 0.1%-CHO-containing feed. In the coronary artery, a remarkable thickening of intima with accumulation of foam cells and a marked stenosis of coronary space were observed in chicks fed on 1.0%-CHO-containing feed. The results of present study indicate that the chicken can be a useful experimental animal in the study of hypercholesterolemia, hyperlipidemia and atherosclerosis. No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254　 96 1 2007 Role of the Surface IgM-Inducing Factor in Development of B Lymphocytes in Chicken Embryonic Bursa of Fabricius 55 58 EN Song Han Asaki Abe Kiyoaki Narahara Yasuhiro Kondo 　The bursa of Fabricius plays essential roles in the establishment of immune functions of avian species as a primary site for differentiation and proliferation of B lymphocytes. The bursa of chick embryos is colonized by lymphoid cell precursors only between the days 7th of embryogenesis (E7) and E14. Susceptibility to the sIgM-inducing factor may fluctuate in bursal lymphoid cells during the lymphoid precursor cell-receptive period. In the present study, the dynamic changes in the sIgM-positive ratio and responsiveness to sIgM-inducing factor were examined in lymphoid cells sampled from the bursa during the B precursor cell-receptive period (E10 to E13) and findings suggest that responsiveness to sIgM-inducing factor varies with the development of the chick embryos. E11 is suggested to be a critical stage of B-lymphocytegenesis in the bursa of chick embryos. No potential conflict of interest relevant to this article was reported.