胆汁成分と肝臓組織 第3編 胆汁酸に作用させた肝組織についての検討

I have observed the activity of the dehydrating enzyme of lactic acid at the surface of the liver tissue and the prosperity and decay of mitochondria after shaking the cutting tissue of the liver with the physiologic saline solution, the bile and the natrium choleric acid solution into the physiologic saline solution. The results are as follows: 1) The activity of the dehydrating enzyme of lactic acid in the tissue is not influenced within four hours on the shaking of the liver tissue in the physiologic saline solution. 2) The activity of the dehydrating enzyme especially the dehydrating enzyme of lactic acid become depressed, as the density of the choleric acid natrium solution reaches over a certain level and the acting time is prolonged, on the shaking of the liver tissue with the natrium choleric acid solution into the physiologic saline solution, but it is markedly lighter than that by the bile. At that time, there is not any difference between the sodium glycocolic acid and the sodium taurocholic acid. And the fall of those enzymes activities remained at the 3 or 4 layers of the liver cells in depth. 3) The remakable fall of the enzymes activities by the bile is partly associated with the obstructing action by the natrium choleric acid, but it seems to be mostly caused by the reduction of biliverdin to bilirubin at the inside and outside of the tissue. 4) The liver cells at the surface mades a degeneratien by the process of times, the mitochondria changes the form, the movement of the brown becomes dull and then disappears on the shaking of the liver tissue with the phyiologic saline solution, but this process makes promoted by the use of the natrium choleric acid solution into the physiologic saline solution and it become the most high and fast by the bile. Since the aboves, it can be said that the choleric acid acts to the mitochondria of the liver cells and gives a influence to the activity of the enzyme.