start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=527 end-page=541 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Antitumor effects and augmentation of cellular immunity by Schizophyllan and Bestatin kn-title=SchizophyllanとNK-421の抗腫瘍,延命効果と細胞性免疫能増強効果 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Antitumor activities and effects on cellular immunity of Schizophyllan (SPG), a highly purified polysaccharide extracted from the culture filtrate of Schizophyllum commune Fries, and Bestatin (NK-421), an inhibitor of aminopeptidase B discovered in the culture broth of Streptomyces olivoreticuli, were investigated. The optimal dose of SPG monotherapy to manifest an antitumor effect on MH-134 hepatomas transplanted into C3H/He mice was 10mg/kg, both intraperitoneally (i.p.) and subcutaneously (S.C.). The optimal timing of SPG monotherapy was observed in the group which was treated from 1 day after tumor transplantation, resulting in the most prolonged mean survival (MS) and the highest survival rate. In combined therapy with SPG and mitomycin C (MMC), the most prolonged MS was observed in the group which was treated with MMC on day 3 after tumor transplantation and combined with SPG from day 5. Moreover, the effectiveness continued when the same combined therapy was conducted after the removal of transplanted tumors. The highest ADCC activity by spleen cells harvested from the mice in the SPG monotherapy group was obtained on day 12 after tumor transplantation. The ADCC activity tended to become lower in the MMC monotherapy group, but in the combined therapy group with optimal timing, the highest ADCC activity mediated by non-adherent cells was observed on day 14 after tumor transplantation. Suppressed NK cell activity in tumor-bearing mice was increased to normal levels by SPG monotherapy. On the other hand, NK 421 monotherapy did not manifest a satisfactory antitumor effect regarding survival rate, although it potentiated ADCC activity over a wide range of doses, particularly 5 and 10mg/kg. The main cellular component exhibiting ADCC activity induced by NK 421 therapy seemed to be non-T-cells. By NK-421 monotherapy, the suppressed NK cell activity in tumor-bearing mice was restored up to the normal level. en-copyright= kn-copyright= en-aut-name=OkaTetsuhide en-aut-sei=Oka en-aut-mei=Tetsuhide kn-aut-name=岡哲秀 kn-aut-sei=岡 kn-aut-mei=哲秀 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科学教室 en-keyword=シゾフィラン kn-keyword=シゾフィラン en-keyword=NK-421 kn-keyword=NK-421 en-keyword=SPG kn-keyword=SPG en-keyword=ベスタチン kn-keyword=ベスタチン END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=515 end-page=526 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Reverse tolerance by chronic methamphetamine and stress induced response -Behavioral and biochemical changes in response to footshock kn-title=メタンフェタミン逆耐性とストレス負荷時の反応 ―Foot shockによる行動及び生化学的変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of repeated methamphetamine (MAP) treatment (4mg/kg per day for 14 days) on behavioral and neurochemical changes induced by footshock stress in rats were examined. The challenge by footshock was conducted 7 days after cessation of the chronic treatment. The duration of fighting behavior resulting from the footshock (2.5mA, 5Hz, 10min) was significantly longer in the MAP-treated rats than in controls. After the footshock stress, the number (Bmax) of specific [(3)H] -spiperone binding sites in the striatum decreased significantly in the MAP-treated rats. No differences were found between the MAP-treated rats and controls in the concentrations of monoamines and their metabolites in the frontal cortex, striatum and mesolimbic areas. en-copyright= kn-copyright= en-aut-name=FujiwaraYutaka en-aut-sei=Fujiwara en-aut-mei=Yutaka kn-aut-name=藤原豊 kn-aut-sei=藤原 kn-aut-mei=豊 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部神経精神医学教室 en-keyword=メタンフェタミン kn-keyword=メタンフェタミン en-keyword=フットショックストレス kn-keyword=フットショックストレス en-keyword=モノアミン kn-keyword=モノアミン en-keyword=トリチウムスペピロン結合 kn-keyword=トリチウムスペピロン結合 en-keyword=闘争行動 kn-keyword=闘争行動 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=503 end-page=514 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cytotoxicity of regional lymph nodes lymphocytes from patients with colon cancer kn-title=大腸癌患者領域リンパ節リンパ球の殺細胞性に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Twenty-nine patients with colon cancer who underwent operations at the First Department of Surgery, Okayama University Medical School were studied. During operations, regional lymph nodes were collected and classified into four groups; groups N(1, 2, 3, and 4) Tumor grades followed Dukes classification. Cytotoxicity of the lymph node lymphocytes in each group and of the peripheral blood lymphocytes (PBL) in each patient was examined. Cell lines of P-4788 isolated from the metastaic ascites of the patients with colon cancer were used as target cells for examining cytotoxicity of the lymph nodes lymphocytes and PBL. As the lymph node lymphocytes and PBL were closer to cancer lesions, their cytotoxicity tended to decrease and was significantly different between N(1 , 2, and 3) groups and N(4) group and between N(1, 2, and 3) groups and PBL group (p<0.01). As the cancer progressed, the cytotoxicity decreased and was significantly different between Dukes A, B, and C groups and Dukes D group p<0.01). Compared between groups of negative and positive metastatic lymph nodes, the cytotoxicity of lymph node lymphocytes and PBL tended to decrease in the positive group, but it was not significantly different. When the tumor of 8 cm in diameter was made to be border for tumor classification, cytotoxicity of PBL and regional lymph node lymphocytes decreased as the diameter of the tumor became greater and significantly different (p<0.01). en-copyright= kn-copyright= en-aut-name=HiguchiYasuhiro en-aut-sei=Higuchi en-aut-mei=Yasuhiro kn-aut-name=樋口康彦 kn-aut-sei=樋口 kn-aut-mei=康彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学第1外科 en-keyword=大腸癌 kn-keyword=大腸癌 en-keyword=TPLA kn-keyword=TPLA en-keyword=リンパ節 kn-keyword=リンパ節 en-keyword=リンパ球の殺細胞性 kn-keyword=リンパ球の殺細胞性 en-keyword=細胞性免疫 kn-keyword=細胞性免疫 en-keyword=IgG Fc Positive T-cell kn-keyword=IgG Fc Positive T-cell END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=493 end-page=501 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Lymphocyte subsets in the peripheral blood and liver of patients with acute exacerbation of chronic hepatitis B and of patients treated with adenine arabinoside (Ara-A) kn-title=B型慢性肝炎における急性増悪時およびadenine arabinoside(Ara-A)の投与前後の末梢血中ならびに肝組織中リンパ球サブセットの検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The lymphocyte subsets in the peripheral blood and liver biopsy material of 25 patients with HBe antigen-positive type B chronic hepatitis were studied by an indirect immunoperoxidase-labeled antibody method using monoclonal antibodies to surface antigens on pan T cells (Leu-1+), cytotoxic/suppressor T cells (Leu-2a+), helper/inducer T cells (Leu-3a+), natural killer/killer cells (Leu-7+) and B cells (Leu-10+). The percentage of circulating cytotoxic/suppressor cells decreased during acute exacerbation of chronic hepatitis B. In contrast, the percentage of intrahepatic cytotoxic/suppressor cells increased during acute exacerbation. In the liver biopsy specimens taken during acute exacerbation, numerous lymphocytes infiltrated into sites of hepatocytic necrosis, and the majority of these cells had a cytotoxic/suppressor phenotype (Leu-2a+). These findings suggest that T cell cytotoxicity plays an important role in the mechanism of liver cell damage during exacerbations of chronic hepatitis B. The lymphocyte subsets in the blood and in the liver biopsy material of 4 patients with chronic hepatitis B obtained about 2-7 weeks before and after treatment with Ara-A were studied in the same way. In both the peripheral blood and liver, the percentage of cytotoxic/suppressor cells was decreased after the treatment. Most of the patients showed a decrease in serum HBV-associated DNA polymerase activity and HBV antigens (HBsAg and HBcAg) in the liver. These findings suggest that as a result of the reduction of HBV replication due to the anti-viral effect of Ara-A, the T cell cytotoxicity is diminished after treatment with Ara-A. en-copyright= kn-copyright= en-aut-name=HyodoIchinosuke en-aut-sei=Hyodo en-aut-mei=Ichinosuke kn-aut-name=兵頭一之介 kn-aut-sei=兵頭 kn-aut-mei=一之介 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1内科学教室 en-keyword=B型慢性肝炎 kn-keyword=B型慢性肝炎 en-keyword=リンパ球サブセット kn-keyword=リンパ球サブセット en-keyword=酵素抗体法 kn-keyword=酵素抗体法 en-keyword=Ara-A kn-keyword=Ara-A END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=481 end-page=491 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on low back pain among workers in a train repair factory -The incidence and occupational cause of low back pain and the influence of low back pain on activities in daily life- kn-title=鉄道車両整備工場における職業性腰痛の衛生学的研究 ―腰痛の発症状況および腰痛が日常生活に及ぼす影響について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Owing to the changes in working conditions brought about by mechanization, the occupational causes of low back pain (LBP) have shifted from dynamic factors to static ones. In the train repair factory, however, the workers must operate in such narrow working spaces that it is difficult to use machines. Therefore, the workers must often move heavy materials by hand and perform their duties in unnatural postures. The author deals with health problems induced by LBP among workers engaged in locomotive repair. All of the workers (178) were examined by questionnaire, and 120 workers who were suffering from LBP or had taken sick leave on account of LBP were summoned for a medical examination. The questionnaire consisted of twenty items specially selected to evaluate the influence of LBP on activities in daily life. Twenty-five workers (14.0%) complained of continuous LBP, and 89 workers (50.0%) complained of occasional LBP. The workers in this factory had a higher prevalence of LBP than the workers in many other factories. The rate of complaints concerning LBP rose according to the increase in the frequency of unnatural postures and the handling of heavy materials during work. It was demonstrated that LBP had a harmful influence on activites in daily life. en-copyright= kn-copyright= en-aut-name=ArisawaToyotake en-aut-sei=Arisawa en-aut-mei=Toyotake kn-aut-name=有沢豊武 kn-aut-sei=有沢 kn-aut-mei=豊武 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部衛生学教室 en-keyword=職業性腰痛 kn-keyword=職業性腰痛 en-keyword=重量物の取り扱い kn-keyword=重量物の取り扱い en-keyword=不自然な作業姿勢 kn-keyword=不自然な作業姿勢 en-keyword=日常生活の不便苦痛 kn-keyword=日常生活の不便苦痛 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=475 end-page=480 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of left inferior vena cave kn-title=左側下大静脈の一例 en-subtitle= kn-subtitle= en-abstract= kn-abstract=We found a case of left-sided inferior vena cava(ivc) by chance upon upper abdominal CT examination. Left-sided ivc rarely presents clinical symptoms, but it is necessary to distinguish ivc from abnormal masses such as retroperitoneal tumors or lymphnodal swelling. We discussed left-sided ivc and the literature about it. en-copyright= kn-copyright= en-aut-name=HashimotoKeiji en-aut-sei=Hashimoto en-aut-mei=Keiji kn-aut-name=橋本啓二 kn-aut-sei=橋本 kn-aut-mei=啓二 aut-affil-num=1 ORCID= en-aut-name=KimotoShin en-aut-sei=Kimoto en-aut-mei=Shin kn-aut-name=木本真 kn-aut-sei=木本 kn-aut-mei=真 aut-affil-num=2 ORCID= en-aut-name=JoujaIkuo en-aut-sei=Jouja en-aut-mei=Ikuo kn-aut-name=上者郁夫 kn-aut-sei=上者 kn-aut-mei=郁夫 aut-affil-num=3 ORCID= en-aut-name=YamamotoYoshio en-aut-sei=Yamamoto en-aut-mei=Yoshio kn-aut-name=山本淑雄 kn-aut-sei=山本 kn-aut-mei=淑雄 aut-affil-num=4 ORCID= en-aut-name=UedaHiroyuki en-aut-sei=Ueda en-aut-mei=Hiroyuki kn-aut-name=上田裕之 kn-aut-sei=上田 kn-aut-mei=裕之 aut-affil-num=5 ORCID= en-aut-name=KajiMitsumasa en-aut-sei=Kaji en-aut-mei=Mitsumasa kn-aut-name=加地充昌 kn-aut-sei=加地 kn-aut-mei=充昌 aut-affil-num=6 ORCID= en-aut-name=ShirakamiToshiaki en-aut-sei=Shirakami en-aut-mei=Toshiaki kn-aut-name=白神敏明 kn-aut-sei=白神 kn-aut-mei=敏明 aut-affil-num=7 ORCID= en-aut-name=HirakiYoshio en-aut-sei=Hiraki en-aut-mei=Yoshio kn-aut-name=平木祥夫 kn-aut-sei=平木 kn-aut-mei=祥夫 aut-affil-num=8 ORCID= en-aut-name=AonoKaname en-aut-sei=Aono en-aut-mei=Kaname kn-aut-name=青野要 kn-aut-sei=青野 kn-aut-mei=要 aut-affil-num=9 ORCID= en-aut-name=SugitaKatsuhiko en-aut-sei=Sugita en-aut-mei=Katsuhiko kn-aut-name=杉田勝彦 kn-aut-sei=杉田 kn-aut-mei=勝彦 aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=9 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=10 en-affil= kn-affil=香川県立中央病院放射線科 en-keyword=左側下大静脈 kn-keyword=左側下大静脈 en-keyword=腹部CT kn-keyword=腹部CT END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=465 end-page=473 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Integration of HBV DNA in hepatocellular carcinoma kn-title=肝細胞癌におけるHBVDNAの組込み en-subtitle= kn-subtitle= en-abstract= kn-abstract=In order to clarify the relationship between hepatocellular carcinoma (HCC) and integration of hepatitis B virus (HBV) DNA into the host hepatocyte genome, the states of HBV DNA in 14 cases of HCC, 3 cases of chronic hepatitis, 2 cases of metastatic hepatic carcinoma and 4 normal livers were examined by the Southern blot hybridization technique. All 3 cases of HCC positive for serum HBsAg had both free and integrated HBV DNA. Integration of HBV DNA without free HBV DNA was detected in 2 cut of 4 HCC cases negative for serum HBsAg but positive for more than one of the HBV-related antibodies. Only free HBV DNA was observed in all 3 cases of chronic hepatitis positive for serum HBsAg. The remaining cases had no HBV DNA in their liver tissues. en-copyright= kn-copyright= en-aut-name=HadaHajime en-aut-sei=Hada en-aut-mei=Hajime kn-aut-name=羽田元 kn-aut-sei=羽田 kn-aut-mei=元 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1内科教室 en-keyword=HBVDNA kn-keyword=HBVDNA en-keyword=hepatocellular carcinoma kn-keyword=hepatocellular carcinoma en-keyword=Southern transfer kn-keyword=Southern transfer END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=451 end-page=464 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A study on the progress of children with attention deficit disorder (ADD) -Analysis of the problems of adaptation in daily and school life- kn-title=注意集中障害児の経過に関する研究 ―日常・学校生活への適応について― en-subtitle= kn-subtitle= en-abstract= kn-abstract=A questionnaire study was conducted on children with attention deficit disorder (ADD) in order to clarify their problems of adaptation in daily and school life, and to note the differences in their maladaptation according to their age. Questionnaires concerning daily life were mailed to the parents of 157 children diagnosed as ADD at the Kochi Prefectutural Mental Health Center between 1973 and 1982. Sixty-nine parents replied. Questionnaires concerning school life were mailed to 57 teachers of ADD children, who had previously received the parents permission to respond to these quesionnaires. Forty teachers sent back replies. The subjects were divided into 2 or 3 groups according to their age. In daily life, the number of children capable of coordinated motions, shopping by themselves and playing a role in games, was greater in the high-age group than in the low-age group. Hyperactivity was not observed among the school children, and this was attributed to a certain improvement in their condition before they entered shool. On the other hand, distractibility was observed frequently in all the age groups. In school life, rudeness and distractibility were observed more frequently in the high-age group than in the low-age group. On the other hand, restlessness was less frequent in the high-age group than in the low-age group. ADD-children had a high prevalence, more than 60% , of misbehavior such as temper tantrums, braking rules and criticizing other children excessively in their interpersonal relationships. The number of children with normal abilities of drawing and manual arts was greater in the high-age group than in the low-age group. More than 70% of all the children had attained elementary abilities of reading and writing. But 50-67.5% of the ADD-children had poor school records, and the high-age group received low marks more frequently than the low-age group. A higher prevalence of perinatal disorders was observed among the ADD-children, but there was no relationship between this prevalence and the degree of their maladaptation in daily and school life. Their IQ level was found to have no relation to their school records, and it was demonstrated that the earlier the first consultation was conducted, the more positive was the effect on the children's adaptation. en-copyright= kn-copyright= en-aut-name=MinoYoshio en-aut-sei=Mino en-aut-mei=Yoshio kn-aut-name=三野善央 kn-aut-sei=三野 kn-aut-mei=善央 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部衛生学教室 en-keyword=注意集中障害 kn-keyword=注意集中障害 en-keyword=日常・学校生活 kn-keyword=日常・学校生活 en-keyword=学習障害 kn-keyword=学習障害 en-keyword=学童 kn-keyword=学童 en-keyword=経過 kn-keyword=経過 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=441 end-page=450 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Basophil Reactivity Part II. Observations of reactive basophils by a direct counting method kn-title=好塩基球の反応性に関する研究　第2編　直接算定法による好塩基球の反応性の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Morphological changes of basophils stimulated by anti-IgE, anti-IgG and an specific antigen were observed in 62 asthmatic and 7 healthy subjects by direct counting. The percentage of anti-IgE induced reactive basophils determined by the direct counting method was similar to that by the smear method. Basophil reactivity to both anti-IgE and anti-IgG was low in the healthy subjects. Basophils from asthmatic subjects with a high serum IgE level reacted strongly to anti-IgE. The optimal concentration of house dust for observing reactive basophils was a 10(2)-fold dilution of the extract. The percentage of reactive basophils induced by house dust correlated with the skin end point, the RAST score and the bronchial challenge test in asthmatic subjects sensitive to the corresponding antigen. These results suggest that observation of morphological changes by the direct counting method is useful for in vitro detection of specific antigens. en-copyright= kn-copyright= en-aut-name=GodaYoshinori en-aut-sei=Goda en-aut-mei=Yoshinori kn-aut-name=合田吉ｺ kn-aut-sei=合田 kn-aut-mei=吉ｺ aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=反応好塩基球 kn-keyword=反応好塩基球 en-keyword=直接算定法 kn-keyword=直接算定法 en-keyword=抗ヒトIgE kn-keyword=抗ヒトIgE en-keyword=特異抗原 kn-keyword=特異抗原 en-keyword=気管支喘息 kn-keyword=気管支喘息 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=429 end-page=440 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Basophil Reactivity Part I. Observations of reactive basophils by a smear method kn-title=好塩基球の反応性に関する研究　第1編　塗沫標本による好塩基球の反応性の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Morphological changes of basophils stimulated by anti-IgE and anti-IgG were observed in 73 asthmatic and 10 healthy subjects by smear specimen. Basophil reactivity to both anti-IgE and anti-IgG was low in the healthy subjects. Basophils from atopic asthmatics reacted to anti-IgE more strongly than the cells from the healthy subjects, while the basophil response to anti-IgG was low. Basophil reactivity of intractable asthmatics to anti-IgG was higher than the reactivity to anti-IgE. Basophils from asthmatic subjects with a high serum IgE level reacted to anti-IgE strongly, while the cells from subjects with a low serum IgE level (less than 100IU/ml) showed a high response to anti-IgG. In the cases with a basophil ratio of less than 0.5, basophils released a large amout of histamine by stimulation with anti-IgE. The release of histamine induced by anti-IgE was low in the cases with low reactive basophils. These results suggest that observation of morphological changes of basophils stimulated by anti-IgE and anti-IgG is useful for the clinical study of bronchial asthma. en-copyright= kn-copyright= en-aut-name=GodaYoshinori en-aut-sei=Goda en-aut-mei=Yoshinori kn-aut-name=合田吉ｺ kn-aut-sei=合田 kn-aut-mei=吉ｺ aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=好塩基球の反応性 kn-keyword=好塩基球の反応性 en-keyword=抗ヒトIgE kn-keyword=抗ヒトIgE en-keyword=抗ヒトIgG kn-keyword=抗ヒトIgG en-keyword=気管支喘息 kn-keyword=気管支喘息 END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=419 end-page=428 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Molecular cloning of the SV40 small t antigen gene and purification of the antigen kn-title=SV40小型腫瘍抗原遺伝子のクローン化と抗原の精製 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The simian virus 40 (SV40) DNA fragment encoding small t antigen was cloned with expression vector pUC8 for the purification of small t-antigen. Small t antigen produced in Escherichia coli was a hybrid protein of 22,000 daltons which comprised about 6% of the total protein. Hybrid small t antigen maintained antigenic activity which was examined by Western-blotting and enzyme-linked immunoassay. Hybrid small t antigen was extracted from E. coli with urea and purified from a small t-antigen band in SDS-polyacrylamide gel. The purified antigen maintained antigenic activity. en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name=池田正五 kn-aut-sei=池田 kn-aut-mei=正五 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=SV40 t 抗原 kn-keyword=SV40 t 抗原 en-keyword=遺伝子のクローン化 kn-keyword=遺伝子のクローン化 en-keyword=発現ベクター kn-keyword=発現ベクター en-keyword=ウエスタン・ブロッティング kn-keyword=ウエスタン・ブロッティング END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=409 end-page=417 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cytological diagnosis of lung cancer II. A combination of aerosol induction and Saccomanno's concentration method for sputum cytology kn-title=肺癌の診断に関する研究　第2編　喀痰誘発法とSaccomanno法の併用による喀痰細胞診 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The usefulness of combining aerosol induction with Saccomanno's concentration method was examined in 240 patients who were diagnosed as having lung cancer by fiberoptic bronchoscopy and /or by thoracotomy. By the combined method, satisfactory sputum was obtained from 97.1% of the patients. Sputum cytology gave a positive diagnosis in 57.5% of the patients. Positive rates were 84.8% for patients with centrally located lung cancer and 38.3% for patients with peripherally located lung cancer. With regard to histological classification, positive rates of sputum cytology were 36.9% for adenocarcinomas, 76.8% for squamous cell carcinomas and 73.3% for small cell carcinomas. This combined method resulted in a improved yield of satisfactory sputum as well as positive cytology compared with a conventional method using spontaneous sputum, which gave only 23.3% positive cytology. The combined method may be useful for the diagnosis of lung cancer in mass surveys. en-copyright= kn-copyright= en-aut-name=TamuraTetsuo en-aut-sei=Tamura en-aut-mei=Tetsuo kn-aut-name=田村哲生 kn-aut-sei=田村 kn-aut-mei=哲生 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=Lung cancer kn-keyword=Lung cancer en-keyword=Diagnosis kn-keyword=Diagnosis en-keyword=Sputum cytology kn-keyword=Sputum cytology END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=399 end-page=408 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cytological diagnosis of lung cancer I. Bronchoalveolar lavage in the diagnosis of peripheral lung cancer kn-title=肺癌の診断に関する研究　第1編　病巣気管支洗浄法による末梢型肺癌の診断 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Bronchoalveolar lavage and subsequent bronchial brushing techniques were examined in 60 consecutive cases of endoscopically non-visible peripheral lung cancer in an attempt to improve the diagnostic rate. Lavaged fluid was processed routinely for cytological examinations, and carcinoembryonic antigen (CEA) concentrations were measured concomitantly. Lavaged fluid gave a cytological diagnosis in 63.3% of the patients. The positive cytology rate by brushing was 76.7% . Of 14 patients with negative cytology on brushing, 6 were positive in lavaged fluid. The combination of bronchoalveolar lavage and subsequent bronchial brushing gave a cytological diagnosis in 86.7% of the patients. CEA concentrations of lavaged fluid of patients with lung cancer were higher than those of patients with non-malignant pulmonary diseases. It may be said that increased CEA concentrations in lavaged fluid suggest lung cancer. The relatively low positive rate (76.7%) by brushing cytology appeared due to a technical problem that the preceding bronchoalveolar lavage made it difficult to accomplish a precise brushing under fluoroscopy. Accordingly, the sequence of the two techniques was reversed thereafter. Of 40 consecutive cases with endoscopically non-visible peripheral lung cancer, brushing cytology gave a diagnosis in 87.5% , and bronchoalveolar lavage did so in 65.0% . The combination of bronchial brushing and subsequent bronchoalveolar lavage gave a cytological diagnosis in 92.5% of the patients. In conclusion, bronchoalveolar lavage was useful in the diagnosis of patients with peripheral lung cancer. en-copyright= kn-copyright= en-aut-name=TamuraTetsuo en-aut-sei=Tamura en-aut-mei=Tetsuo kn-aut-name=田村哲生 kn-aut-sei=田村 kn-aut-mei=哲生 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科教室 en-keyword=Lung cancer kn-keyword=Lung cancer en-keyword=Diagnosis kn-keyword=Diagnosis en-keyword=Bronchoalveolar lavage kn-keyword=Bronchoalveolar lavage END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=385 end-page=397 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Arterial terminals in the rat spleen. A scanning electron microscopic study of perfusion-fixed, freeze-cracked tissue blocks. kn-title=ラット脾臓の動脈終止,灌流固定試料の走査電子顕微鏡観察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rat spleens were perfused arterially with physiological saline and glutaraldehyde, freeze-cracked in liquid nitrogen, conductively stained with tannin-osmium-thiocarbohydrazide-osmium, critical-point-dried with liquid carbon dioxide, and observed under a scanning electron microscope. The terminals of the follicular arteries ended in the interstitial tissue spaces between the marginal zone and white pulp, and those of the penicillar arteries ended in the interstitial tissue spaces of the Billroth cords. Only a faw connections of the arterial terminals with the venous sinuses were noted. These findings strongly support the "open theory" that in the mammalian spleen the arterial blood mainly flows into the tissue spaces before being received by the venous sinuses. en-copyright= kn-copyright= en-aut-name=AkitaShinji en-aut-sei=Akita en-aut-mei=Shinji kn-aut-name=秋田真治 kn-aut-sei=秋田 kn-aut-mei=真治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部解剖学第二講座 en-keyword=ラット脾臓 (rat spleen) kn-keyword=ラット脾臓 (rat spleen) en-keyword=動脈端 (arterial terminals) kn-keyword=動脈端 (arterial terminals) en-keyword=開放説 (open theory) kn-keyword=開放説 (open theory) en-keyword=閉鎖説 (closed theory) kn-keyword=閉鎖説 (closed theory) en-keyword=灌流固定 (perfusion fixation) kn-keyword=灌流固定 (perfusion fixation) en-keyword=走査電顕観察 (scanning electron microscopy) kn-keyword=走査電顕観察 (scanning electron microscopy) END