start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=359
end-page=368
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on ferrous ion-induced lipid peroxidation Part Ⅱ Analysis of the induction period (lag) of ferrous ion-induced lipid peroxidation
kn-title=二価鉄誘導脂質過酸化反応に関する研究 第二編 二価鉄誘導脂質過酸化反応における誘導期(lag)の二価鉄の消長による解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The author measured simultaneously Fe(Ⅱ)-induced peroxidation of lipid extracted from rat liver mitochondria by the TBA method and the concentration of Fe(Ⅱ) added to the medium quantitatively using Nitoroso-PSAP reagent. 1) An induction period (lag) at the early phase of the reaction of Fe(Ⅱ)-induced lipid peroxidation was observed as well as in mitochondria. 2) After most of the Fe(Ⅱ) concentration had decreased, the TBA-value, after lagtime, increased sharply. 3) Phosphate added to the medium shortened the lag-time. The lag-time in the presence of phosphate varied with the concentration and pH. However, in each case, there was a correlation between the decrement of Fe(Ⅱ) concentration and the length of lag time as described above. 4) By adding EDTA for various reaction times, Fe was chelated immediately, then the TBA value did not increase. 5) The results suggest: iron interacted with the chain reaction of lipid peroxidation, mainly as Fe(Ⅱ) and/or partially Fe(Ⅲ); Fe(Ⅱ) maintains the lag as a inhibitory action to the resolution into malondialdehyde(MDA) from lipid peroxide; Fe(Ⅲ) was required for the formation of MDA. 6) Using these results, the author discussed the mechanisms of Fe(Ⅱ)-induced lipid peroxidation.
en-copyright=
kn-copyright=

en-aut-name=HashimotoKeizi
en-aut-sei=Hashimoto
en-aut-mei=Keizi
kn-aut-name=橋本啓二
kn-aut-sei=橋本
kn-aut-mei=啓二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=ミトコンドリア脂質
kn-keyword=ミトコンドリア脂質
en-keyword=脂質過酸化反応
kn-keyword=脂質過酸化反応
en-keyword=鉄
kn-keyword=鉄
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=349
end-page=357
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on ferrous ion-induced lipid peroxidation Part IQuantitative analysis of ferrous ion in a system of ferrous ion-induced lipid peroxidation
kn-title=二価鉄誘導脂質過酸化反応に関する研究 第一編 二価鉄誘導脂質過酸化反応溶液中の二価鉄の定量
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe(Ⅱ). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe(Ⅱ) quantitatively and selectively the system of Fe(Ⅱ)-induced lipid peroxidation, and obtained the following results. 1) Fe(Ⅱ) could be measured quantitatively without reductant. The Fe(Ⅱ) complex had a molar absorptivity of 4.5×10(4)dm(3) mol(-1) cm(-1) at 756 nm. 2) Fe(Ⅱ) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe(Ⅱ) complex had a molar absorptivity of 3.14×10(4)dm(3) mol(-1) at 756nm. 3) Fe(Ⅱ) could be measured quantitatively and selectivily under conditions involving a mixture of Fe(Ⅱ).
en-copyright=
kn-copyright=

en-aut-name=HashimotoKeizi
en-aut-sei=Hashimoto
en-aut-mei=Keizi
kn-aut-name=橋本啓二
kn-aut-sei=橋本
kn-aut-mei=啓二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=鉄定量
kn-keyword=鉄定量
en-keyword=脂質過酸化反応
kn-keyword=脂質過酸化反応
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=341
end-page=348
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=On the proliferation regulatory mechanism of spontaneously transformed L cell(L-N)
kn-title=自然形質転換したL細胞(L-N)の増殖制御機構について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=1) The L-0 cell absolutely required serum for proliferation, but L-N cell grew rapidly in serum free medium. 2) L-N cell cultured in MEM medium with TPB reached high saturation density as in serum containing medium. 3) The growth rate of L-N cell in serum free medium was dependent on initial density of cell population just before logarithmic growing phase. 4) L-N cell rounded cell cycle from G-1 phase to S phase 4 hours faster than L-0 cell.
en-copyright=
kn-copyright=

en-aut-name=MatsuiHideki
en-aut-sei=Matsui
en-aut-mei=Hideki
kn-aut-name=松井秀樹
kn-aut-sei=松井
kn-aut-mei=秀樹
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一生理学教室
en-keyword=無血清
kn-keyword=無血清
en-keyword=細胞増殖制御
kn-keyword=細胞増殖制御
en-keyword=L細胞
kn-keyword=L細胞
en-keyword=形質転換
kn-keyword=形質転換
en-keyword=DNA合成
kn-keyword=DNA合成
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=331
end-page=339
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of N-acetoxy-methylaminoazobenzene and 3'-methyl-4-dimethylaminoazobenzene on cloned rat liver cells with special reference to cellular morphology and growth capacity
kn-title=N-acetoxy-methylaminoazobenzeneのクローン化されたラット肝細胞株に対する影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cloned rat liver cells were treated with an ultimate carcinogen, N-acetoxy-methylaminoazobenzene(AcMAB) and 3'-methyl-4-dimethylaminoazobenzene(3'-Me-DAB) then various biological characters of the cells including tumorigenicity were examined. In regard to back-transplantation, after 11 days of experiments, a high concentration (5x10(-4)M) of AcMAB-treated cells formed tumors in one out of two rats. And a low concentration(1x10(-4)M) of AcMAB or 3'-Me-DAB-treated cells and untreated control cells did not form tumors. After 99 days of experiments, tumor formation was observed not only with the high concentration of AcMAB-or 3'-Me-DAB-treated cells but also with untreated control cells. AcMAB-treated cells had altered morphology and growth properties in vitro compared to 3'-Me-DAB-treated cells and untreated control cells. These results suggest an ultimate character of AcMAB, especially from the view point of tumor-forming capacity.
en-copyright=
kn-copyright=

en-aut-name=NishiyamaSyoichi
en-aut-sei=Nishiyama
en-aut-mei=Syoichi
kn-aut-name=西山正一
kn-aut-sei=西山
kn-aut-mei=正一
aut-affil-num=1
ORCID=

en-aut-name=TokiwaTakayoshi
en-aut-sei=Tokiwa
en-aut-mei=Takayoshi
kn-aut-name=常盤孝義
kn-aut-sei=常盤
kn-aut-mei=孝義
aut-affil-num=2
ORCID=

en-aut-name=SatoJiro
en-aut-sei=Sato
en-aut-mei=Jiro
kn-aut-name=佐藤二郎
kn-aut-sei=佐藤
kn-aut-mei=二郎
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌研病理部

affil-num=2
en-affil=
kn-affil=岡山大学医学部癌研病理部

affil-num=3
en-affil=
kn-affil=岡山大学医学部癌研病理部
en-keyword=Ac2F細胞
kn-keyword=Ac2F細胞
en-keyword=ラット肝単個培養株
kn-keyword=ラット肝単個培養株
en-keyword=in vitro化学発癌
kn-keyword=in vitro化学発癌
en-keyword=AcMAB
kn-keyword=AcMAB
en-keyword=DAB代謝活性体
kn-keyword=DAB代謝活性体
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=315
end-page=330
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study of dopamine receptors in the rat central nervous system Part 2. Regulation of dopamine receptors in the rat striatum after long-term treatment with antiparkinsonian drugs or neuroleptics.
kn-title=中枢神経系のdopamine受容体に関する研究 第2編 抗パーキンソン病薬ならびに抗精神病薬反復投与によるdopamine受容体の調節
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Long-term drug studies in animals using antiparkinsonian drugs (L-dopa and bromocriptine) and neuroleptics (chlorpromazine and haloperidol) can provide valuable clinical information on the mechanism of drug action and can contribute to a better understanding of the side effects of such therapy. Moreover, it has been suggested that the molecular action of these drugs involves dopamine receptors in the brain. Recently, it was proposed that the dopamine receptor exists in two forms: a D(1)-dopamine receptor, which is coupled to dopamine sensitive adenilate cyclase, and a D(2)-dopamine receptor, which is uncoupled. I have established D(1)-dopamine-radioreceptor assay and D(2)-dopamine-radioreceptor assay using [(3)H]-dopamine as a ligand, then I studied the effects of chronic administration of these drugs on two forms of dopamine receptors. It is well known that most patients on prolonged L-dopa therapy either become refractory to the drug, or develop "on-off" phenomena or dyskinesias. In this study, an increase in high affinity binding sites of D(1)-dopamine receptor was detected, whereas D(2)-dopamine receptor subsensitivity was observed following long-term L-dopa therapy. On the other hand, after bromocriptine treatment for 14days, the high affinity binding sites of D(1)-and D(2)-dopamine receptors were increased in the rat striatum. The finding that long-term bromocriptine therapy increases D(1)-and D(2)-dopamine receptors may explain, in part, the efficacy of combination bromocriptine and L-dopa treatment. After chlorpromazine treatment for 14 days, the high affinity binding sites of D(1)-and D(2)-dopamine receptors were slightly increased in rat striatum, and after haloperidol treatment for 14 days, the high affinity binding sites of D(2)-dopamine receptor were also slightly increased in rat striatum. Therefore, it was suggested that symptoms of extrapyramidal side effects after long-term neuroleptic treatment reflect a functional supersensitivity of dopamine receptors in the rat striatum.
en-copyright=
kn-copyright=

en-aut-name=YamawakiYasuhide
en-aut-sei=Yamawaki
en-aut-mei=Yasuhide
kn-aut-name=山脇泰秀
kn-aut-sei=山脇
kn-aut-mei=泰秀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科学講座
en-keyword=L-dopa
kn-keyword=L-dopa
en-keyword=bromocriptine
kn-keyword=bromocriptine
en-keyword=chlorpromazine
kn-keyword=chlorpromazine
en-keyword=haloperidol
kn-keyword=haloperidol
en-keyword=D(1)-dopamine受容体
kn-keyword=D(1)-dopamine受容体
en-keyword=D(2)-dopamine受容体
kn-keyword=D(2)-dopamine受容体
en-keyword=受容体のsupersensitivity
kn-keyword=受容体のsupersensitivity
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=301
end-page=314
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A study of dopamine receptors in the rat central nervous system Part 1. Biochemical and pharmacological properties of dopamine receptors.
kn-title=中枢神経系のdopamine受容体に関する研究 第1編 dopamine受容体の生化学的ならびに薬理学的検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Until recently, it was widely accepted that neuroleptics act by blocking the dopamine receptor. Many investigators have done binding studies in vitro using [3H]-neuroleptics as ligands. However, recent studies on rat brains suggest that [3H]-neuroleptics label not only the dopamine receptor, but other receptors as well. In this study, the cross reactivities of numerous centrally acting drugs in dopamine-radioreceptor assay were different from those in spiroperidol-radioreceptor assay. Manganese enhanced the specific binding of [3H]-dopamine dramatically by 300% even at low concentrations, although manganese did not influence the specific binding of [3H]-spiroperidol. To differentiate dopamine and spiroperidol receptors in the rat brain, I solubilized these receptor molecules by treatment with detergent and examined the molecular weight by gel filtration. As compared with the elution volumes of marker proteins, the approximate molecular weight of solubilized dopamine receptors were about 200,000 dalton (Stokes' radius: 5.0nm) in the absence of manganese, and about 400,000 dalton (Stokes'radius: 6.1nm) in the presence of manganese. Moreover, manganese was found in the same fraction as the solubilized dopamine receptor. However, the molecular weight of the solubilized spiroperidol receptor was about 120,000 dalton (Stokes'radius: 4.3nm) in both the presence and absence of manganese. These data suggest that manganese couples specifically with manganese-free dopamine receptors, and that this manganese - dopamine receptor complex may have a strong capacity to bind dopamine. On the other hand, the receptor activities of spiroperidol were independent of manganese. These findings suggest that the site of action of spiroperidol is a receptor different from authentic dopamine receptor.
en-copyright=
kn-copyright=

en-aut-name=YamawakiYasuhide
en-aut-sei=Yamawaki
en-aut-mei=Yasuhide
kn-aut-name=山脇泰秀
kn-aut-sei=山脇
kn-aut-mei=泰秀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第三内科学講座
en-keyword=dopamine受容体
kn-keyword=dopamine受容体
en-keyword=spiroperidol受容体
kn-keyword=spiroperidol受容体
en-keyword=〔(3)H〕-dopamine
kn-keyword=〔(3)H〕-dopamine
en-keyword=[(3)H〕-spiroperidol
kn-keyword=[(3)H〕-spiroperidol
en-keyword=molecular weight
kn-keyword=molecular weight
en-keyword=central nervous system
kn-keyword=central nervous system
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=289
end-page=300
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Peripheral blood flow measured by a newly developed non-invasive and quantitative blood flow meter (QFM) and its clinical use. The blood flow of peripheral arteries, and the change of flow after experimental replacement of a prosthesis
kn-title=新しく開発された非観血的血流計による末梢動脈血流量と臨床応用-総頸動脈,大腿動脈,足背動脈の血流量と実験的人工血管置換前後の血流変化-
en-subtitle=
kn-subtitle=
en-abstract=A newly developed non-invasive and quantitative blood flow meter (QFM) was used to measure blood flow in human peripheral arteries (common carotid artery, femoral artery, dorsal pedal artery), and to determine hemodynamic differences after replacement of the canine abdominal aorta with a prosthesis. The results obtained were as follows: 1) Blood flow of the canine femoral artery by QFM correlated well with that obtained by an electromagnetic flow meter with a correlation coefficient (r) of 0.94 (p<0.001) (y=1.05x-12.9). 2) In normal subjects, blood flow of the common carotid, femoral and dorsal pedal arteries changed with age, namely, the blood flow of the common carotid artery decreased after the age of 40 with a statistically significant difference and that of the femoral artery after the age of 60. 3) The blood flow and velocity in the femoral artery and dorsal pedal arteries increased markedly with exercise. 4) The effect of the prosthetic valve in the mitral position on the aortic flow was determined in the common carotid artery. However, there was no significant difference. 5) Blood flow and velocity increased after the aorta was replaced with a prosthesis, although there was no significant difference. It was concluded in the present study that non-invasiveness and the ability of QFM to detect fine changes in flow patterns would be an ideal tool for diagnosing and following up patients with vascular disease.
kn-abstract=非観血的な方法による血流計測は,循環系疾患の診断,治療および術後のfollow upなどにますますその重要性を増してきている.ことに末梢動脈の閉塞性疾患への応用はいろいろに試みられている.しかし超音波ドプラ法を応用した非観血的血流計による血流情報は,今日なお多くの問題点を残しており,完成された検査法とは言いがたい.たとえば超音波ドプラ法による計測は超音波ビームと血流走行とのなす角度が不明のため血流速度の絶対値の計測は不可能であつた.この欠点を解消するため血流走行と角度依存性のない超音波ドプラ法による血流計測法が開発された.定量的非観血的血流計(QFM)と呼ばれる新らしい装置で,血流速度の絶対値のみならず血流量も測定可能である.これは血管径の変化を経時的に計測する超音波パルスエコートラッキング法と3枚のトランスジューサーを使用することにより角度依好性をなくした血流計の2系統からなり(図1),これらをマイクロコンピューターで有機的に組み合わせたものである.すなわちこの血流計は流速に血管径を加味したもので,従来の血流計に血管径という一次元を加えたもので,この方法によりはじめて血流量の測定が可能になったといえよう.しかし本計測器は開発されてからの日時は短かく,その精度に関しては実験的にも充分な検証があるとはいえない.本研究はこのQFM法の精度を検するのを目的としたものである.犬を用いた実験では,大腿動脈の流量を本法で計測し,電磁流量計で計測した流量と比較した.臨床では,健常者で総頸動脈,大腿動脈,足背動脈の血流量や流態性状の計測をおこない,大腿動脈,足背動脈においては運動負荷による計測もおこなった.さらに人工弁置換患者の総頸動脈流量や流態性状も計測し,犬でも腹部大動脈の人工血管置換による流量変化や流態性状を計測し,人工血管置換による血流変化を検討した.
en-copyright=
kn-copyright=

en-aut-name=NakasoneKoji
en-aut-sei=Nakasone
en-aut-mei=Koji
kn-aut-name=仲宗根浩二
kn-aut-sei=仲宗根
kn-aut-mei=浩二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2外科教室
en-keyword=非観血的血流計
kn-keyword=非観血的血流計
en-keyword=流量
kn-keyword=流量
en-keyword=流速
kn-keyword=流速
en-keyword=血管径
kn-keyword=血管径
en-keyword=壁偏位幅
kn-keyword=壁偏位幅
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=269
end-page=287
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on renal disease in rheumatoid arthritis. Part 2. Renal biopsy in rheumatoid arthritis patients with renal disorders.
kn-title=慢性関節リウマチにおける腎病変の研究 第2編 腎病変を合併した慢性関節リウマチ患者の腎生検による検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Renal biopsies were performed on seven patients with rheumatoid arthritis (RA) and proteinuria. The material was examined by light and immunofluorescent microscopy. Two of the biopsy specimens were examined by electron microscopy. Membranous nephropathy was found in six of the seven specimens. Brilliant, fine granular fluorescence with IgG was present along the glomerular capillary loops under immunofluorescent microscopy. These findings suggested that these renal disorders were due to an immune complex mechanism. The proteinuria of five patients indicated that the membranous nephropathy occurred following D-penicillamine treatment or chrysotherapy. In one patient, there was no evidence of a relationship between D-penicillamine and proteinuria from her history. The remaining patient had a mild cell proliferative glomerulonephritis under light microscopy. Fluorescence was not found under immunofluorescent microscopy. For this reason, it seemed that the renal disorder was not caused by D-penicillamine, althoug her history showed that D-penicillamine brought on proteinuria. These findings suggested that renal biopsy is desirable in patients with long term proteinuria, when the proteinuria is clearly caused by D-penicillamine or gold salts. Furthermore, it seemed that long term proteinuria due to D-penicillamine, was probably caused by an immunological mechanism. The seven patients showed no evidence of secondary amyloidosis due to RA.
en-copyright=
kn-copyright=

en-aut-name=UdaShinichi
en-aut-sei=Uda
en-aut-mei=Shinichi
kn-aut-name=宇田慎一
kn-aut-sei=宇田
kn-aut-mei=慎一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部大藤内科教室
en-keyword=慢性関節リウマチ
kn-keyword=慢性関節リウマチ
en-keyword=膜性糸球体腎炎
kn-keyword=膜性糸球体腎炎
en-keyword=腎障害
kn-keyword=腎障害
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=257
end-page=267
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on renal disease in rheumatoid arthritis. Part 1. Renal function, mainly serum and urine β(2)-microglobulin concentrations.
kn-title=慢性関節リウマチにおける腎病変の研究 第1編 血中および尿中β(2)-microglobulinを中心とした慢性関節リウマチ患者の腎機能
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Serum, urine and synovial fluid β(2)-microglobulin (BMG) was measured by radioimmunoassay (Phadebas β(2)-micro test) in 43 rheumatoid arthritis (RA) patients being treated with gold salts, in 9 patients with osteoarthritis (OA) and in 9 patients with renal disease. The relationships between BMG, RA activity index and renal function were investigated. The serum BMG level in the renal disease group was significantly inversely correlated with creatinine clearance. The 43 rheumatoid arthritis patients were divided into a proteinuria group and a non-proteinuria group. The serum BMG level was higher in the renal disease group than in the proteinuria group, and was higher in the proteinuria group than in the non-proteinuria group. The synovial gluid BMG level was measured in 16 patients with RA and in the 9 patients with OA. This measure was higher in the RA group than in the OA group. The 43 RA patients were divided into positive and negative groups by C-reactive protein, RA test results (erythrocyte sedimentation rate over 50 mm/h and under 49 mm/h), and γ-globulin levels (over 1.5 g/dl and under 1.49 g/dl). Serum BMG levels were compared in these two groups. They tended to be higher in the higher activity groups than in the lower activity groups. From these results, a serum BMG level below 3.15 μg/ml (which is higher than in healthy normals) was considered "normal" for RA. The non-proteinuria group was investigated in a 2×2 table divided at 3.15 μg/ml serum BMG level and 500 μg/l urinary BMG level. There were two patients with abnormal BMG levels in both serum and urine samples, two patients with abnormal levels in the serum sample, and two patients with abnormal BMG levels in only the urine sample. It was thought that some patients without proteinuria in crysotherapy started to show renal damage. The results suggest that during gold treatment RA patients require not only urinalysis, but measurements of serum and urinary BMG levels for possible renal damage.
en-copyright=
kn-copyright=

en-aut-name=UdaShinichi
en-aut-sei=Uda
en-aut-mei=Shinichi
kn-aut-name=宇田慎一
kn-aut-sei=宇田
kn-aut-mei=慎一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部大藤内科教室
en-keyword=慢性関節リウマチ
kn-keyword=慢性関節リウマチ
en-keyword=尿細管障害
kn-keyword=尿細管障害
en-keyword=β(2)-microglobulin
kn-keyword=β(2)-microglobulin
en-keyword=金療法
kn-keyword=金療法
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=247
end-page=255
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on thrombocytes in patients with rheumatoid arthritis Part 2. Platelet specific protein in patients with rheumatoid arthritis.
kn-title=慢性関節リウマチにおける血小板に関する研究 第2編 慢性関節リウマチにおける血小板特異蛋白
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Plasma β-thromboglobulin (β-TG) concentration and platelet factor 4 (PF(4)) activity were examined and related to parameters of inflammation in patients with rheumatoid arthritis (RA). In 42 RA patients, the β-TG concentration assessed by radioimmunoassay (β-TG RIA KIT Amersham) was significantly higher than that of 17 normal controls (p<0.002). The β-TG concentration correlated with the platelet count (γ=0.68, p<0.01) and erythrocyte sedimentation rate (γ=0.32, p<0.005). Plasma PF(4) activity, tested by heparin neutralizing activity, was significantly higher than that of 17 normal controls (p<0.001). In the strongly positive CRP group (CRP 3(+) or more) PF(4) activity was significantly higher than in the weakly positive CRP group (less than CRP 2(+)) (p<0.05). No correlation between PF(4) activity and other parameters such as the platelet count, erythrocyte sedimentation rate, fibrinogen, antithrombin Ⅲ, or FDP was demonstrated. These results suggest that accerelated platelet functions were present in patients with clinically active RA.
en-copyright=
kn-copyright=

en-aut-name=IshibeYoji
en-aut-sei=Ishibe
en-aut-mei=Yoji
kn-aut-name=石部陽二
kn-aut-sei=石部
kn-aut-mei=陽二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第3内科学教室
en-keyword=慢性関節リウマチ
kn-keyword=慢性関節リウマチ
en-keyword=血小板特異蛋白
kn-keyword=血小板特異蛋白
en-keyword=β-thromboglobulin
kn-keyword=β-thromboglobulin
en-keyword=血小板第4因子
kn-keyword=血小板第4因子
en-keyword=炎症の指標
kn-keyword=炎症の指標
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=235
end-page=246
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on thrombocytes in patients with rheumatoid arthritis Part 1. Platelet abnormalities in patients with rheumatoid arthritis and adjuvant arthritic rats.
kn-title=慢性関節リウマチにおける血小板に関する研究 第1編 慢性関節リウマチ及びアジュバント関節炎ラットにおける血小板の量的質的異常
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The platelet count and platelet adhesiveness were examined and comparing with other parameters of inflammation in patients with rheumatoid arthritis (RA) and in adjuvant arthritic rats. The platelet count in 75 patients showed good correlation with the erythrocyte sedimentation rate (γ=0.41, p<0.01). The platelet adhesiveness of 40 RA patients tested by the glassbeads method showed a significantly increased retention rate compared with that of 16 normal controls (p 0.005). In RA patients, platelet adhesiveness was significantly greater in the strongly positive CRP group (CRP 5(+) or more) than in the weakly positive CRP group (CRP 1(+) and 2(+)) (p<0.005). In adjuvant arthritic rats, the platelet count and adhesiveness increased for 5 weeks after a one shot injection. Furthermore, good correlations were noted between the platelet count and platelet adhesiveness (γ=0.72, p<0.005), platelet count and paw size (γ=0.79, p<0.001), and platelet adhesiveness and paw size (γ=0.57, p<0.001). These data suggest that platelet count and adhesiveness would be one of the good parameters of inflammation in RA patients.
en-copyright=
kn-copyright=

en-aut-name=IshibeYoji
en-aut-sei=Ishibe
en-aut-mei=Yoji
kn-aut-name=石部陽二
kn-aut-sei=石部
kn-aut-mei=陽二
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第3内科学教室
en-keyword=慢性関節リウマチ
kn-keyword=慢性関節リウマチ
en-keyword=血小板数
kn-keyword=血小板数
en-keyword=血小板粘着能
kn-keyword=血小板粘着能
en-keyword=炎症の指標
kn-keyword=炎症の指標
en-keyword=アジュバント関節炎
kn-keyword=アジュバント関節炎
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=221
end-page=234
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Fundamental Study and Clinical Evaluation of Residual Liver Function after Hepatectomy Predicted by Liver-scintigraphy
kn-title=肝臓シンチグラフィーによる残存肝機能の推定に関する基礎的研究ならびに臨床例についての検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Part 1 A method of evaluating the effective liver volume has been devised for liver-scintigraphy. Matrix ROI (region of interest) was set on the anterior liver scintigram with Au-198 colloid and the depths under matrix ROI were calculated by the use of the plateau values of the histograms and the absorption coefficient (formula 2). The effective liver volume was the sum total of the areas of matrix ROI to the depths. The effective liver volume was calculated as follows: 1=∫(w)(o)Ioe(-μx)dx............(1) Io: γ-ray intensity of unit depth  Ip: γ-ray intensity of w depth  w: depth of radioactive substance  μ: absoption coifficient  therefore w=-1/μIn{1-Ip/Io(1-e(-μwo)}.............(2) Ipo: γ-ray intensity of standard depth  wo: depth corresponding Ipo and the effective liver volume is  V=Σ(s×wi)............(3) s: area of metrix ROI
In order to check the accuracy of this method, this was performed - the liver phantom, the dog's liver- and the patient's liver-scintigraphy. The volume obtained for the liver phantom was nearly equal to the real volume and the dog's was almost equivalent to the weight of it. The volume for every patient showed good correlation with the volume as measured by CT scan. Part 2 Liver-scintigraphy were performed in 14 preoperative patients of hepatectomy. The estimated volume of residual liver and the total effective liver volume were evaluated, and the accumulation rates of Au-198 colloid in the total liver (K) and those in the estimated residual liver (K(1)) were determined. The operation was done and 3 weeks later the same examination was performed. The following calculation was studied as the index of resectable liver volume.
(estimated volume of residual liver×K1)/(total effective volume×normal K*)  *0.184 (determined in our laboratory)
The calculation values for two of 14 patients who developed liver failure after hepatic resection were 0.205 and 0.361, and for others without severe complications were more than 0.384. The results for small numbers of patients suggest that this calculation may be useful for determining the indications for hepatectomy. In regard to regeneration of the liver, only the ratio of the effective liver volume after right lobectomy (including extended right lobectomy) to it before appeared to be related to the K of preoperation.
en-copyright=
kn-copyright=

en-aut-name=TamaiToyosato
en-aut-sei=Tamai
en-aut-mei=Toyosato
kn-aut-name=玉井豊理
kn-aut-sei=玉井
kn-aut-mei=豊理
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=Liver-scintigraphy Matrix ROI
kn-keyword=Liver-scintigraphy Matrix ROI
en-keyword=Effective Liver Volume
kn-keyword=Effective Liver Volume
en-keyword=Predicted Residual Liver Function
kn-keyword=Predicted Residual Liver Function
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=211
end-page=220
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Changes in hematologic parameters of dog due to exposure to trichloroethylene and 1,1,1-trichloroethane
kn-title=麻酔性有機溶剤トリクロロエチレンおよび1,1,1-トリクロロエタン急性暴露時における血液系値の変動
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Dogs were exposed to either trichloroethylene(TCL) or 1,1,1-trichloroethane(TCE) in order to investigate the effects of these solvents on hematologic parameters. Further-more, because of unusual changes in white blood cell counts following exposure of pregnant dogs to these solvents, investigation of the effects of several hormones given prior to exposure to the solvents was pursued. The results obtained were as follows. 1. White blood cell counts showed a marked decrease immediately after starting exposure to TCL or 1,1,1-TCE, though the decrease was temporary. On the other hand red blood cell counts, hematocrit values and blood platelet counts showed no changes. 2. While each kind of white blood cell decreased in number, the degree was greatest in neutrophils. 3. TCL caused a greater decrease in white blood cell counts than 1,1,1-TCE. 4. A dose-response relationship was seen between the concentrations of 1,1,1-TCE from 200 to 700 ppm and the decrease in white blood cell counts. 5. No change in white blood cell counts was observed following the exposure of pregnant dogs to 1,1,1-TCE. 6. Premeditation with estradiol, progesterone and hydrocortisone had a preventive effect on the decrease in white blood cell counts caused by the exposure to 1,1,1-TCE, but no such effect was seen with premedication of testosterone.
en-copyright=
kn-copyright=

en-aut-name=HirotaSeiro
en-aut-sei=Hirota
en-aut-mei=Seiro
kn-aut-name=廣田晴郎
kn-aut-sei=廣田
kn-aut-mei=晴郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第2外科学教室
en-keyword=麻酔性有機溶剤
kn-keyword=麻酔性有機溶剤
en-keyword=血液系値の変動
kn-keyword=血液系値の変動
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=201
end-page=210
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=EAC Rosette Inhibition for Detecting Immune Complexes in Renal Transplant Patients
kn-title=腎移植患者におけるEACロゼット阻止試験を用いたImmune Complex測定の試み
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In human renal allografts, recipients respond with various specific immune reactions, such as the production of immune complexes. The circulating immune complexes provoke damage of the grafted kidney under the appropriate condition. Therefore, the detection of circulating immune complexes is important in predicting the rejection of grafted kidneys. Several methods of measuring immune complexes have been reported. In this study, sera from 25 patients who had received renal allografts were studied for the presence of circulating immune complexes by using a EAC rosette inhibition test. Sera from 25 patients were divided into three groups (1. acute rejection, 2. chronic rejection, 3. no rejection episode) and EAC rosette inhibition tests performed. Serum samples from the chronic rejection group gave high inhibition of EAC rosette formation, showing good correlation with clinical chronic rejection episodes. It was concluded that circulating immune complexes play an important role in chronic rejection. Therefore, the EAC rosette inhibition test is useful for predicting chronic rejection, and measuring circulating immune complexes.
en-copyright=
kn-copyright=

en-aut-name=FujiwaraTouru
en-aut-sei=Fujiwara
en-aut-mei=Touru
kn-aut-name=藤原徹
kn-aut-sei=藤原
kn-aut-mei=徹
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=腎移植
kn-keyword=腎移植
en-keyword=拒絶反応
kn-keyword=拒絶反応
en-keyword=EACロゼット阻止試験
kn-keyword=EACロゼット阻止試験
en-keyword=Immune Complex
kn-keyword=Immune Complex
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=189
end-page=200
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Intra-operative evaluation of left ventricular function determined by epicardial echocardiography. Effects of myocardial preservation on diastolic cardiac function.
kn-title=心外膜心エコー法による開心術中左心機能評価の実験的試み ―拡張期心機能からみた心筋保護の意義―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Intra-operative left ventricular functions were measured by epicardial echocardiography, which was obtained by small ultrasound transducer (5 megaheltz) on the epicardium of the right ventricular outflow. Canine left cardiac functions after cardiopulmonary bypass were compared in 2 groups, one being 60-minute cardiac arrest with cardioplegia (group A) and the other 45-minute ischemic arrest (group B). The results obtained were as follows: 1. It was simple and reliable to determine the state of left ventricular function with epicardial echocardiography during operation. 2. There was good correlation between the values for cardiac output as measured by thermodilution and calculated by epicardial echocardiography. 3. Contractility after cardiopulmonary bypass was well maintained in both groups. Some indices of systolic cardiac function in group B after cardiopulmonary bypass were compromised, but those of group A remained normal. 4. The posterior left ventricular wall motion in diastole in group B was slower than that in group A after cardiopulmonary bypass. Ischemic arrest (group B) was shown particularly to impair myocardial relaxation and to raise left ventricular wall stress in diastole. It was concluded in the present study that impairment in cardiac function appeared first in diastole following cardiac surgery and that epicardial echocardiography would be a convenient and simple tool for determining cardiac function during surgical intervention. It was also found that cardioplegia preserved cardiac function near normal level even in diastole.
en-copyright=
kn-copyright=

en-aut-name=SuzukiYoshihide
en-aut-sei=Suzuki
en-aut-mei=Yoshihide
kn-aut-name=鈴木芳英
kn-aut-sei=鈴木
kn-aut-mei=芳英
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科学教室
en-keyword=心外膜心エコー図
kn-keyword=心外膜心エコー図
en-keyword=心筋保護
kn-keyword=心筋保護
en-keyword=拡張期心機能
kn-keyword=拡張期心機能
en-keyword=左室後壁拡張速度
kn-keyword=左室後壁拡張速度
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=179
end-page=188
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effect of 3'-methyl-4-dimethylaminoazobenzene(3'-Me-DAB) in the presence and absence of liver microsomes(S15 mixture) on cloned rat liver cell line derived from a rat fed 4-dimethylaminoazobenzene Part 2 The accumulation effect and histological examination of 3'-Me-DAB treated cells
kn-title=培養肝細胞の3'-Me-DAB処理による細胞悪性度の増強に関する研究 第2編 3'-Me-DAB処理の加算効果および病理組織学的検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=I reported previously that cell malignancy was promoted significantly by treatment with 3'-Me-DAB in the presence and absence of S15 mixture. In this report, I examined the effect of treatment frequency of 3'-Me-DAB in the presence and absence of S15 mixture on a cloned rat liver cell line (CL-20) which had low-tumor producing capacity. Tumors produced by subcutaneous back-transplantation of CL-20 cells treated with 3'-Me-DAB in the presence and absence of S15 mixture showed more undifferentiated, solid and infiltrative morphology than that of non-treated control cells. The tumors produced by back-transplantation of CL-20 cells treated with high concentrations of 3'-Me-DAB showed metastatic growth in the lung of rats, but those produced by back-transplantation of untreated CL-20 cells did not. Plating efficiency increased in proportion to the treatment frequency of 3'-Me-DAB in the presence and absence of S15 mixture. This fact suggests that plating efficiency is effective as a marker for the promotion of 3'-Me-DAB-induced cell malignancy. The aggregate sizes formed by the rotation culture were larger in the cells treated with 3'-Me-DAB in the presence and absence of S15 mixture than the control cells. However, the effect of the treatment frequency of 3'-Me-DAB was rarely found in this experiment. Few changes were observed with other in vitro markers for the promotion of cell malignancy. These findings suggested that cell malignancy could be promoted by repeated treatments of 3'-Me-DAB in the presence or absence of S15 mixture in vitro.
en-copyright=
kn-copyright=

en-aut-name=NishiyamaSyoichi
en-aut-sei=Nishiyama
en-aut-mei=Syoichi
kn-aut-name=西山正一
kn-aut-sei=西山
kn-aut-mei=正一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部癌研病理部
en-keyword=発癌剤の処理回数
kn-keyword=発癌剤の処理回数
en-keyword=S15 mixture
kn-keyword=S15 mixture
en-keyword=病理組織学的所見
kn-keyword=病理組織学的所見
en-keyword=腫瘍の転移
kn-keyword=腫瘍の転移
en-keyword=Plating efficiency
kn-keyword=Plating efficiency
END

start-ver=1.4
cd-journal=joma
no-vol=94
cd-vols=
no-issue=3-4
article-no=
start-page=165
end-page=177
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1982
dt-pub=19820430
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Study of leukocyte adherence inhibition test in lung cancer.
kn-title=白血球粘着阻止試験による肺癌患者の免疫能測定に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The significance of immunity in lung cancer patients has remained unclear. In this report, the relationship between the leukocyte adherence inhibition test in glass test tubes (tube LAI test) which seemed to be a tumorspecific immune response and the delayed hypersensitivity(DH) skin test was studied. The tissue extracts from squamous cell carcinoma, adenocarcinoma of the lung and adenocarcinoma of the colon were prepared for the tube LAI test on 40 lung cancer patients. Thirty-two patients out of 40 were also examined by a battery of DH skin tests including PPD, Candida, SK-SD and PHA. The follwoing results were obtained. 1. Lung cancer patients showed a significantly high nonadherence index (NAI), calculated from the difference of the numbers of non-adherent cells in the presence of tissue extracts, and 35 per cent of them were positive for the tube LAI test. 2. The positive cases with the tube LAI test seemed to react more specifically with extracts from the same histological type. 3. Eight cases out of 13 (61.5%) in stage I and Ⅱ, and 6 cases out of 27 (22.2%) in stage Ⅲ and Ⅳ were positive for the tube LAI test. Early stage cases had a high incidence of positive tube LAI test. 4. Eleven cases out of 13 (85%) with positive tube LAI tests showed normal DH skin reactions. On the other hand, 11 cases out of 16 (69%) with positive DH skin tests were related to positive tube LAI tests.
en-copyright=
kn-copyright=

en-aut-name=WadaToyoji
en-aut-sei=Wada
en-aut-mei=Toyoji
kn-aut-name=和田豊治
kn-aut-sei=和田
kn-aut-mei=豊治
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二外科教室
en-keyword=白血球粘着阻止試験
kn-keyword=白血球粘着阻止試験
en-keyword=ガラス試験管内白血球粘着阻止試験
kn-keyword=ガラス試験管内白血球粘着阻止試験
en-keyword=非粘着細胞指数
kn-keyword=非粘着細胞指数
en-keyword=皮膚反応指数
kn-keyword=皮膚反応指数
en-keyword=遅延型皮膚反応
kn-keyword=遅延型皮膚反応
END