岡山医学会Acta Medica Okayama0030-1558925-61980腎疾患と溶連菌感染 第2編 IgA腎症における溶連菌感染の研究801813ENMakotoHiramatsuIgA nephropathy has been recognized increasingly as a distinct clinicopathological entity. We studied 53 patients with IgA nephropathy immunologically, and clarified a certain relationship between this condition and streptococcal infection. Twenty-three of 42 patients (55%) had the preceeding upper respiratory tract infections. The mean serum concentration of IgA was 323 mg/dl, and 14 of 36 patients (39%) had greater than 350 mg/dl. Anti-streptolysin O titer was elevated in 4 of 47 patients (9%), anti-streptokinase titer in 5 of 33 patients (15%), and anti-hialuronidase titer in 4 of 35 patients (11%). We estimated the titer of antibody to C-carbohydrate derived from streptococcal cell walls which was supposed to persist longer than the antibodies to streptococcal extracellular products. The extraction of C-carbohydrate antigen, i.e. a group specific antigen of streptococcus, was carried out by a modified method of Slade. The quantitative determination of antibody by O-stearoyl C-carbohydrate-sensitized red cell hemmagglutination showed significant high titers in 43% of patients with IgA nephropathy. The results were as follows: titers in the serum of normal adults ranged below 1:16; in IgA nephropathy, between 1:2 and 1:512; in other types of chronic glomerulonephritis, between 1:2 and 1:32; and in anaphylactoid purpura nephritis, between 1:8 and 1:1024. The data above suggested a close relationship between IgA nephropathy and streptococcal infection.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980腎疾患と溶連菌感染 第1編 腎疾患における抗レンサ球菌C―多糖体抗体の検討787799ENMakotoHiramatsuFor the purpose of studying the relationship between streptococcal infection and renal diseases, we estimated the titer of antibody to streptococcal C-carbohydrate in serum of patients with various renal diseases. The results were as follows: serum titers were less than 1:8 in normal adults, less than 1:16 in normal children, between 1:16 and 1:1024 in acute glomerulonephritis, between 1: 8 and 1: 1024 in anaphylactoid purpura nephritis, between 1:2 and 1:512 in chronic glomerulonephritis, between 1:4 and 1:8 in primary nephrotic syndrome, and between 1:2 and 1:16 in lupus nephritis. This results showed significant high titers in acute glomerulonephritis, anaphylactoid purpura nephritis and chronic glomerulonephritis, suggesting a close relationship between these diseases and streptococcal infection. The titers in patients with IgA-nephropathy which was included in chronic glomerulonephritis in the present study were higher than in patients with other groups of chronic glomerulonephritis. This may suggest that streptococcal infection plays an etiologic role in IgA-nephropathy.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980ヒト白血病性B細胞株に関する研究 第2編 ヒト白血病性B細胞株（BALL-1株）のハムスターへの継代移植775785ENKazuoNakamuraA human leukemic B-cell line, which was designated as BALL-1, was recently established in our laboratory. In this experiment, BALL-1 cells were serially transplanted into antilymphocyte serum (ALS)-treated newborn Syrian hamsters. When 1.0-8.5×10(7) viable cells were inoculated intraperitoneally, all of them developed invasive tumors 12-26 days after implantation. All the hamsters except one had 0.2-8.0ml of milky ascites containing 0.9-11.0×10(8) tumor cells/ml. Histologically, invasion of the tumor cells was observed in many abdominal organs and in subcutaneous tissue. Progression to leukemia was common (24/25), and the percentage of the tumor cells in the peripheral blood ranged between 2 and 91% . Morphological features, surface markers, and chromosome constitutions of the tumor cells were identical to those of BALL-1 cells. These findings show thast BALL-1 cells were serially transplantable into the experimental animals possessing the characteristics of human leukemic B-cells. To our knowledge, this is the first report of successful heterotransplantation of a human leukemic B-cell line. This animal model will provide a new advantage in many aspects of leukemia research.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980ヒト白血病性B細胞株に関する研究 第1編 ヒト白血病性B細胞株(BALL-1株)の樹立とその性状763773ENKazuoNakamuraA human B-cell line designated as BALL-1 was established from the peripheral blood of a patient with acute lymphoblastic leukemia (ALL). Neither Epstein-Barr virus (EBV) nor its genome was detectable. The morphological and growth characteristics were clearly distinct from those of numerous EBV-positive lymphoblastoid cell lines previously reported. BALL-1 cells probably originated from the donor's leukemic cells judging from their cytogenetic, morphological and surface features. This BALL-1 line is the first EBV-negative B-cell line established from ALL.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980黄色ブドウ球菌リン脂質のミセル構造の特性749762ENKinyaOkuThe ultrastructure of the phospholipid micells (Phosphatidylglycerol: PG, cardiolipin: CL and lysylphosphatidylglycerol: L-PG) from S. aureus were examined by negative staining and freeze-fracture to elucidate the role of each phospholipid in staphylococcal cell membrane. The width of lipid bilayer of each phospholipid was: PG: 40A, CL: 58A and L-PG: 65A, respectively, indicating that the hydration of each lipids was different. Ca(2+) caused the fusion of cardiolipin micell followed by hexagonal-cylindrical comformation. Ca(2+) seemed to give "rigidity" to the cardiolipin bilayer. Mg(2+) modified the cardiolipin lamella causing random-ripple form. Mg(2+) gave "fluidity" to the micellar structure. PG and L-PG were less affected by Ca(2+) or Mg(2+). Conversion of PG to CL was enhanced when S. aureus was exposed to stress condition or when the cell lost the wall. Molar ratio of PG/CL was 5/2 in normal condition, while 2/5 under stress. This adaptational changes of the lipid composition in the membrane may well correlate with the characteristics of cardiolipin micellar structure.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980血清IgEに関する臨床的研究 第1編 気管支喘息患者の血清IgEに関する研究725737ENNobuoUedaSerum IgE level was measured by the method of radioimmunosorbent test (RIST) in 353 subjects including 183 patients with bronchial asthma. One hundred and twenty patients with other pulmonary diseases and allergic diseases, and 50 normal adults for the control. Also, the level of specific IgE antibody to house dust was measured by the method of radioallergosorbent test (RAST) in patients with bronchial asthma. In patients with bronchial asthma, the IgE level was evaluated according to the following conditions, i.e., types by Swinfold's classification, age groups, seasons, peripheral blood counts of basophils and eosinophils and effects by immunotherapy. At the same time, the changes of specific IgE antibody level during the periods of house dust desensitization therapy was evaluated. The relusts were as follows: 1) The normal value of serum IgE level was 189±156 u/ml (mean±one standard deviation) and bronchial asthma patients showed 1121±1147 u/ml, which was remarkably high. And, significantly high values were observed in patients with bronchial asthma, allergic rhinitis, drug allergy, diffuse interstitial pneumonitis and parasite diseases compared to normal control. In chronic bronchitis, pulmonary tuberculosis, pulmonary sarcoidosis, IgE level was about the same as control. 2) Serum IgE level was found to reduce with aging. 3) The seasonal changes of serum IgE level was not significant. 4) Serum IgE level was significantly higher in patients with atpic bronchial asthma than infectious type of bronchial asthma according to Swinfold's classification. 5) The peripheral blood counts of basophils and eosinophils did not correlate significantly with serum IgE level. 6) Serum IgE level after the long term house dust desensitization therapy did not change, but specific IgE antibody was found to be decreased in 2 out of 5 bronchial asthma patients with more than 2+RAST score. 7) In intractable asthmaticus the significant relationship was observed between the clinical improvement by the continuous low dose administration of 6-Mercaptopurine and the decrease of serum IgE level, but the corticosteroids therapy did not affect on the IgE level.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980Conglutinin Solid-phase Radioimmunoassayによる血中immune complexの検出 第1編 基礎的検討703711ENTakahiroTamuraConglutinin solid-phase radioimmunoassay (conglutinin RIA) was utilized for detection of soluble immune complexes in sera. Crude conglutinin was obtained from whole bovine serum using Zymosan as an affinity absorbent. The further purification of crude conglutinin was performed through Sephadex G-200 and DEAE-cellulose column chromatography. The activity of purified conglutinin to haemagglutinate EAC3d was found to be more than 1:2048 in titer. Anti-conglutinin antisera raised in rabbit gave an identical precipitin line against bovine serum and purified conglutinin. Conglutinin RIA was performed using 2.5μg/ml of conglutinin and 25μl of fresh normal human serum as a source of complement. Serum samples were incubated with conglutinin coated on polystylene tubes for 1 hour at room temperature, and incubated with (125)I-labelled anti-human IgG for 4 hours at room temperature. The amounts of immune complexes measurable by this method were in the ranges of 5 to 320μg/ml equivalent to aggregated IgG. Conglutinin RIA was applied to the detection of soluble immune complexes formed in vitro which could be detected both in antigen excess and antibody excess regions.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980Conglutinin Solid-phase Radioimmunoassayによる血中immune complexの検出 第2編 臨床的検討―全身性エリテマトーデスを中心に713723ENTakahiroTamuraThe conglutinin binding activity in sera of the patients with systemic lupus erythematosus (SLE) was measured and following results were obtained. 1. The sera from SLE, progressive systemic sclerosis, mixed connective tissue diseases and Behcet's disease patients had higher conglutinin binding activity than normal human sera. 2. The conglutinin binding activity in SLE showed good correlation with C1q or ds-DNA binding activity (p<0.1) and level of serum CH50, but had poor correlation with staining patterns of antinuclear antibody or clinical nephropathy. 3. Increased activity was found in 33% of SLE sera by the conglutinin radioimmunoassay and 84% of SLE sera by the C1q radioimmunoassay. 4. SLE patients with high conglutinin binding activity were classified into three groups. In the first groups patients has clinically active stage due to SLE, in the second groups patients had no clinical activity during more than several months, and in the third groups patients had no clinical activity due to SLE but had the other clinical manifestations, such as, liver damage. 5. The binding activity in the sera of the SLE patients who had low serum complement levels was not measured by the counglutinin radioimmunoassay.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980血清IgEに関する臨床的研究 第2編 非アトピー性疾患患者の血清IgEに関する研究739748ENNobuoUedaSerum IgE level was measured by the methods of radioimmunosorbent test (RIST) in 116 subjects including 41 patients with malignant lymphomas and 20 patients of myasthenia gravis. In Hodgkin's disease, the serum IgE level was evaluated according to the clinical stages and to the effects of chemotherapy. Also, the changes of serum IgE level was compared before and after thymectomy in myasthenia gravis patients. The results were as follows: 1) Patients with Hodgkin's disease showed remarkably high IgE levels (1726±2180 u/ml). But, serum IgE level in patients with reticulum cell sarcoma, lymphosarcoma, multiple myeloma except IgE myeloma, leukemia and lung cancer was below the normal value. 2) Most of Hodgkin's disease patients with stage V showed high IgE level. 3) Hodgkin's disease patients with mixed cellularity according to Rye's classification showed higher IgE level than other histological types. 4) IgE level in most of Hodgkin's disease patients decreased after chemotherapy. The rebound of IgE level was remarked after cessation of chemotherapy in some of the complete remission patients. 5) The background of allergic disposition was negligible in Hodgkin's disease patients with high IgE level. Most of the patients showed negative reactions of PPD skin test and low blastoid reactivity of lymphocytes by phytohemagglutinin, but the level of IgG, IgA and IgM was not significantly variable. These results suggest that the impaired regulatory functions due to T cells might be a cause of high IgE level in Hodgkin's disease. 6) In 6 cases of myasthenia gravis with proliferative thymoma, the temporary rebound phenomenon of IgE level in 2 months after thymectomy and the gradual decrease of IgE level after 18 months were characteristic. The simultaneous observations of T and B cells suggested that initial phenomenon might be due to the loss of suppressor T cell in the thymus and the second phenomenon might be due to the decrease of helper T cell in the peripheral lymphoid tissues.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980心臓刺激伝導系の組織化学的研究685701ENTatsuhikoMukudaiThe distribution of acid phosphatase and succinic dehydrogenase in the specialized muscle fibers constructing the heart conduction system (sinoatrial node, atrioventricular node, atrioventricular bundle and Purkinje fibers) and the ordinary heart muscle fibers of several mammals were studied histochemically with a light and electron microscopies. The activity of acid phosphatase was high in the specialized muscle fibers of dog and monkey, relatively high in these fibers of rabbit, guinea pig, rat and mouse and low in cow and pig. The activity in the ordinary heart muscle fibers was low in all mammals studied. Embryologic study of the enzyme in rat showed that equal activity was found at the late fetal stage in both heart muscle fibers and increased only in the specialized fibers 30-40 days after birth. Electron microscopic study on the distribution of the enzyme in rat's atrioventricular node showed that the reaction product was found on myofibrils and chromatin in both P cells and T cells which consisted the node. Succinic dehydrogenase activity was studied using mammals described above including cat. The activity in the specialized heart muscle fibers was negative or weakly positive throughout the developing stage of all mammals used, while its activity in the ordinary muscle fibers was already high 1-2 days after birth. From these results, the role of both enzymes in the heart conduction system was discussed.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980超音波ドプラー法と超音波カルディオグラフィ法の併用による非観血的心拍出量測定法675684ENAtsuoOhya近年,方向指示型超音波ドプラー血流計による末梢動脈,ことに総頸動脈の血流波形と心拍出量との関連を分析した報告がみられるようになった.これらの報告は非観血的に超音波ドプラー血流計を用いて,血流速度を連続的に測定しながら負荷を与えたり,術中,術後の心拍出量の変化を経時的に追う方向の有効性を強調している.しかし,こうした測定法では精度に関する限り,非観血的であるという理由からそれほど厳しい要求をなされていない.これらの欠点よりも経時的な相対変化を簡単に,無侵襲で追跡できるという他の追従を許さない特徴を活用できるため,外科臨床においても,術後の患者の経過観察の目的で心拍出量を知るための重要なパラメーターとなりうることによって有力な手段とされてきた.本研究は血流波形の分析のみならず,流量の定量化を試みたもので,超音波ドプラー法と超音波カルディオグラフィの組合せにより,一方の短所を他方の長所で補うことで好結果を得た.超音波ドプラー法で血流速度を経時的に計測し,同時に超音波カルディオグラフィによってその時々の血管径を計測し,波形の積分により流速を算出し,血管径より血管断面積を乗じて血流量の測定を試みた.実験方法は,左総頸動脈流量を中心に行い,流量の基準として電磁流量計を用いた.電磁流量計によって得られた心拍出量と,左総頸動脈の流量比の相関をだし,逆に左頸動脈の流量より心拍出量の推定を行ったものである.実験犬数は15頭で,電磁流量計でえられた左総頸動脈流量は超音波ドプラー法と超音波カルディオグラフィ法によってえられた流量の相関は,y=0.92x-0.03,r=0.96,P<0.001と高い相関関係を示し,さらに,この流量より心拍出量を推定した10例では,y=37.0x+18.9(r=0.90,p<0.001)の結果をえた.本法の臨床応用にあたっては非観血的であるだけに繰返し簡単に,しかも無侵襲に行いうるものであり,術後患者の経過観察のための重要なパラメーターとして好結果を期待できるものである.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980心停止蘇生後の脳障害に関する実験的研究 第2編 バルビタール療法の髄液腔エラスダンスに及ぼす影響について667674ENWasaUedaTo corroborate barbiturate protection from cerebral anoxia after cardiopulmonary arrest, barbiturate therapy was done on 12 adult dogs. Induction of cardiac arrest, cardiopulmonary resuscitation and the measurement of the CSFS-EC, cerebro-spinal fluid space elastic compliance, were done as the same as reported in part one. Values of the arrested group of part one were used as control. Pentobarbital sodium was given intravenously after the reestablishment of the circulation. Twelve animals were devided into two groups, 7 mg/kg and 15 mg/kg of pentobarbital were given to each of 6 animals. Each group of animals were devided into two, again, regarding from the resuscitation time (short ≦ 6 minutes, long ≧ 7 minutes), S-7, S-15, L-7 and L-15 respectively. CSFS-EC of the L-15 increased dramatically at 30 minutes and came down to the control value at one hour. The change of CSFS-EC at 30 minutes of the L-7 was not significantly different from the control. At one hour, CSFS-EC of L-15, L-7 and control were not significantly different from each other. Although CSFS-EC of the control decreased gradually for next 23 hours, L-15, L-7 stayed without significant changes. CSFS-EC of S-15 and S-7 decreased significantly 2 and 4 hours, and returned to the same value as the control group. Barbiturate therapy exaggerate the changes of CSFS-EC and delayed the recovery of the central nervous system from the effect of whole body ischemia and it was hard to find out any reason to do barbiturate therapy after cardiac arrest.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980心停止蘇生後の脳障害に関する実験的研究 第1編 髄液腔エラスタンスの経時的変化について659665ENWasaUedaVolume changes in the central nervous system after cardiac arrest and cardiopulmonary resuscitation were observed by the measurement of cerebro-spinal fluid space elastic compliance (CSFS-EC). Two catheters were placed in the lumbar subarachnoidal space, normal saline solution was injected from one catheter, constantly, and pressure changes were measured with the other. Mongrel dogs, weighing about 10 kg, had cardiac fibrillation induced electrically and maintained for 3 minutes before resuscitation was started. CSFS-EC was measured 30 minutes, one hour, 2 hours, 4 hours and 24 hours after reestablishment of the circulation. In the dogs resuscitated successfully in a short period of time, CSFS-EC decreased gradually and constantly. On the other hand, when the resuscitation took longer, CSFS-EC increased initially, and decreased later. The CSFS-EC at 30 minutes had a good correlation with the time needed for resuscitation. In other words, the longer the time needed for resuscitation the higher the CSFS-EC. One hour after resuscitation, the difference of the CSFS-EC between the long and the short became satistically insignificant. Although 24 hours after resuscitation, all dogs behaved as normal, CSFS-EC were significantly lower than that of control. Even after a short period of cardiac arrest, the recovery of the central nervous system takes longer than expected.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980皮膚電気抵抗減弱部の組織学的研究635657ENYuzuruKurabayashiResearch into the existence of SERDP has been carried out by many workers with a back-ground in modern western medicine. However, it has not yet been possible to prove their existence and there is a great lack of accordance in the arguments presented. The results were as follows: 1) SERDP and Nerve Histology in Each of the Experimental Animal: The local anatomy of the sampling points showed white color nerves in the subcutaneous and muscle tissue. Nerve tissue was present at all SERDP. Nerve bundles were demonstrated at SERIP at the following rates: rabbit 42.9% , and dog, cat, rat and mouse 28.6% . Nerve tissue was most abundant in the subcutaneous tissue, and less so in the muscle layer. Medullated nerve tissue was predominant. Nerve bundle thickness (the following are all average value) were 11×14μ in the dog; cat 8.9×17.0μ, rabbit 8.6×19.4μ, rat 8.4×16.7μ , mouse 4.2×8.9μ and the depth of nerve bundles from the skin surface was: dog 327μ, cat 206.5μ, rabbit 181.3μ, rat 244.5μ and mouse 138.8μ. 2) Relationship between Blood Vessels and SERDP: It was confirmed that 35.9% have a relationship with blood vessels. 3) Relationship of Lymph Vessels to SERDP: 17.9% of SERDP had associated lymph vessels, an association only about 50% of that for blood vessels. 4) Relationship of Connective Tissue to SERDP: Tissue from SERDP and SERIP was taken and stained by the Masson Trichrome method. No specific histological changes were observed. 5) Relationship of Catecholamines to SERDP: A mild increase in catecholamine activity of the SERDP was observed when compared with SERIP. 6) Relationship of SERDP and Macroscopic Vital Staining for Nerve Tissue: Long Chinese acupuncture needles were deeply inserted, excised, and the subcutaneous tissue studied. White nerve fibers were seen to have a fairly high association with SERDP. This was confirmed with vital staining using Leucomethylen blue. 7) Relationship between SERDP and the Phenomenon of Needle Sensation (Tokki, Hibiki): Japanese and Chinese acupuncture needles were placed in SERDP and SERIP in human subject and twirled 5 times to test for the existence of needle sensation. Results were as follows: a) Japanese needles, SERDP 90.5%, SERIP 50.5% b) Chinese needles, SERDP 99.5%, SERIP 65%. From the above, it was demonstrated that macroscopically, histologically, and physiologically, SERDP have an intimate relationship with nerve tissue.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980心拍数と心筋酸素消費量 ―1心拍当りの心筋酸素消費量と心拍数の関係―629633ENShoichiHaraokaDaijiSaitoKazuoIhoriyaHarukiNagahanaAkinobuFujiiIkuzoMashimaRelationship between heart rate and myocardial oxygen consumption per beat was studied in anesthetized open-chest dogs. Myocardial oxygen consumption per minute (MVO(2)) was calculated as the product of the left coronary artery blood flow and coronary arterio-venous oxygen difference. Myocardial oxygen consumption per beat (MVO(2)-Beat) was the quotient of MVO(2) divided by heart rate (HR). HR was varied in 78-210 beats/min with left atrial pacing. Systemic arterial and left ventricular enddiastolic pressure were kept constant, and arterial blood gases were within physiological ranges. Although MVO(2) correlated significantly with HR (r=0.71), MVO(2)-Beat decreased in association with an increase in heart rate. Closer relationship was observed between MVO(2)-Beat and HR; MVO(2)-Beat (ml/100g LV muscle)= -0.00036 + 0.13, r= -0.86, P<0.001. As the results, MVO(2) was represented as a quadratic equation of HR; MVO(2) (ml/min/100g LV muscle)= -0.00036HR(2) + 0.13HR. There was a good correlation (r=0.88) between MVO(2)-Beat and the area under a stroke left ventricular pressure curve. This finding and constant systemic arterial and left ventricular pressure suggest that the inverse relationship between MVO(2)-Beat and HR is mainly due to a reduction in duration of systolic left ventricular wall tension.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980心臓交感神経刺激による心電図変化 (実験的検討)613627ENShoichiHaraokaHidenoriYoshidaKazuoIhoriyaShowzoKusachiOsamuNishiyamaKoichiroYasuharaNobuyukiYamadaTaijiSogoAkinobuFujiiMinoruUedaDaijiSaitoMitsuoHisamatsuElectrocardiographic and vectorcardiographic changes in Frank's corrected orthogonal lead system were studied stimulating electrically the right stellate ganglion (RSG), the left stellate ganglion (LSG) and both stellate ganglia (LSG+RSG) in anesthetized closed-chest dogs. The effects of propranolol on arrhythmias induced by the stimulation of the cardiac sympathetic nerve were also investigated. The following results were obtained: (1) The amplitude of T wave increased in Y lead with simultaneous stimulation of both right and left stellate ganglia. The spatial maximum T vector was displaced inferiorly and increased in the magnitude in all cases. These electrocardiographic changes were similar to those which occurred with LSG stimulation alone. (2) The rotation of the T loop, in the left sagital plane, was clockwise in more than 80% with RSG stimulation, while the T loop following the stimulation of LSG alone and LSG+RSG rotated counterclockwise in more than 75% of all cases. (3) In more than 40% of cases with stimulation of left cardiac sympathetic nerves (LSG, left ventrolateral cervical cardiac nerve), arrhythmias such as A-V dissociation and A-V junctional rhythm were recognized. Stimulation of right cardiac sympathetic nerve (RSG, right recurrent cardiac nerve) markedly increased the rate of sinus rhythm in more than 90% of cases. These arrhythmias resulted from the stimulation of nerves decreased or disappeared after the intravenous injection of propranolol.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980リンパ球混合培養試験の迅速化判定に関する研究597612ENTaizoYamamotoIn the clinic of renal transplantation the difference of histocompatibility matching is significant in judging the prognosis of renal transplantation. MLC test is one of the histocompatibility tests indispensable to select donor-recipient combination, but generally it takes as long as 4-6 days, and it is not easy to use as routine for the practice of urgent renal transplantation. In this study, for the purpose of clinical application of cadaver renal transplantation on the basis of protein synthesis rate with (3)H-leucine pulse label method, this method was used to conduct technical basic experiment for rapid MLC test. First in establishing the condition of culture, Eagle's MEM was used which contains no leucine in the culture, and human serum was not added. The maximum number of reacting lymphocytes in MLC test was 1×10(6)/0.2ml/well. At 24 hours after commencement of culture % SPS (stimulation of protein synthesis) became maximum, and it was decided as time of judgement. There was correlation between rapid and standard MLC tests. % SPS of 10 cases of living donor transplantation was 11.8±16.7, % SPS of 10 cases of cadaver donor transplantation 97.6±31.5, and there was a significant difference (p<0.001). About 9 cases of cadaver kidney transplantation, low HLA matching grade showed high % SPS and a tendency of bad renal function after transplantation. In the cases whose renal function was lost within 3 months after transplantation % SPS tended to be higher than in the cases whose transplanted kidney survived longer. Although there are many factors which influence the prognosis of renal transplantation, it has been suggested that at least such a combination should be selected as % SPS below 80 and HLA matching above 2 matched to maintain good renal function for a long time. From the above, this rapid MLC test has been recognized retrospectively useful to test histocompatibility matching of urgent cadaver renal transplantation.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980ループス腎炎の免疫学的研究 第U編 C(1)qを中心に587595ENYoshinaoTarumiC(1)q, the first component of C(1), was isolated from normal human sera according to Thunild's method. Specific antibody to C(1)q was produced in rabbits. Fluorescein isothiocyanate (FITC) was labelled on the antibody. Renal tissues from 35 patients with systemic lupus erythematosus (SLE) were stained with FITC labelled antibody to C(1)q as well as C(3), IgG, IgM and IgA. The following results were obtained. 1. Glomerular deposition of C(1)q was frequently seen in patients who had massive deposition of immunoglobulins and C(3) along the glomerular basement membrane. The distribution of C(1)q is similar to that of immunoglobulins and C(3). 2. Positive C(1)q deposition on glomeruli was associated with low levels of serum C(1)q and the presence of complement fixing antibody to native DNA. 3. Serial renal biopsy of 15 patients with SLE showed that a marked decrease of C(1)q deposition was observed after steroid therapy. These results indicate that glomerular deposition of C(1)q is of importance to evaluate the clinical and immunopathological activity of lupus nephritis.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980ループス腎炎の免疫学的研究 第1編 DNA抗体の補体合性を中心に575585ENYoshinaoTarumiThe complement fixing activity of antibody to native DNA (CF-n-DNA antibody) was determined in sera of 93 patients with systemic lupus erythematosus (SLE) by complement fluorescent technique and the following results were obtained. 1. The sera of positive CF-n-DNA antibody had significantly higher n-DNA binding and anti complementary activity, lower complement level and mixed type cryoglobulines. 2. The clinical activity of lupus nephritis was correlated with the frequency of CF-n-DNA antibody. Serial studies of 3 patients with lupus nephritis showed a close correlation between the presence of CF-n-DNA antibody and activity of renal disease. 3. The CF-n-DNA antibody was frequently found in patients who had massive deposition of immune complex along the glomerular basement membrane. 4. Sixteen sera of positive CF-n-DNA antibody were treated by DNase. No significant change in the titer of the antibody was obtained before and after DNase treatment. These date suggest that CF-n-DNA antibody was play an important role of pathogenesis in lupus nephritis.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980遅発性ジスキネジア患者のアミン代謝 ―Oxypertine及びHydroxyzine Pamoateの効果より―567573ENTakuoNagaoCerebrospinal fluid HVA, MHPG, 5HIAA, cAMP and cGMP were measured in 12 chronic schizophrenic patients with tardive dyskinesia before and after the administration of oxypertine, hydroxyzine pamoate or placebo. Lumbar puncture was performed after probenecid administration. HVA levels significantly decreased and cAMP levels significantly increased after oxypertine treatment. Three of the four patients treated with oxypertine showed improvement in tardive dyskinesia. 5HIAA levels significantly decreased during hydroxyzine administration. Two of the four patients improved in tardive dyskinesia showed decrease of HVA after hydroxyzine treatment. There were no significant alterations of amine metabolites and cyclic nucleotides in the patients with placebo. Decrease of HVA may indicate the normalization of hyperdopaminergic state. It has been recognized that oxypertine is a noradrenaline depleting agent and hydroxyzine has an antiserotonic and antihistaminic properties. Noradrenaline and/or serotonin may have an effect on dopaminergic function in the central nervous system.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980全身性エリテマトーデスのalternative pathwayに関する研究 ―chemotaxis assayを利用して― 第2編 臨床的考察555565ENTakeoYoshinouchiThe relationship between the classical complement pathway and the alternative complement pathway was studied in 30 SLE patients. The activities of both pathways were measured by chemotaxis assay, and the following results were obtained. 1) The classical pathway-derived chemotactic index (CCI) was correlated with the level of CH50, C4 and C3, but not with those of C5. 2) The alternative pathway-derived chemotactic index (ACI) was correlated with the level of CH50, but not with those of C4, C3 and C5. 3) CCI was positively correlated with ACI. Both indices were low but CCI was more depressed than ACI. These findings suggest that the alternative complement pathway is activated mainly by the C3b positive feedback mechanism which is probably triggered via the classical complement pathway, and that the alternative complement pathway probably play a part in defence mechanism instead of reduced classical complement pathway in SLE.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-1558925-61980全身性エリテマトーデスのalternative pathwayに関する研究 ―chemotaxis assayを利用して― 第1編 alternative pathway測定法の基礎的考察545554ENTakeoYoshinouchiTo measure the activities of classical or alternative complement pathway using the modified Boyden's chemotactic chamber (each compartment 2ml and filter, pore size 3μ), suitable conditions were examined and the following results were obtained. 1) The upper compartment of the chamber contained 4×10(6) polymorphonuclear cells and 10% heat inactivated human serum in medium TC-199. 2) The lower compartment contained 10% fresh human serum as a complement source in medium TC-199. 3) To generate the classical complement pathway-derived chemotactic factor(s), 0.84mg of heat aggregated human Fr.U was added to the lower compartment (4.2mg/ml fresh serum). 4) To generate the alternative complement pathway-derived chemotactic factor(s), 0.2mg of zymosan was added to it (1mg/fresh serum). 5) In this assay, the chemotactic activities derived from classical or alternative complement pathway were dose-dependent upon the serum (complement) concentrations in the lower compartment.No potential conflict of interest relevant to this article was reported.