start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1441
end-page=1446
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=High-performance liquid chromatographic studies of toluene and xylene poisoning Part III: Effects of phenobarbital on metabolism of m-, p-xylene in rats
kn-title=Toluene及びXyleneの中毒に関する高速液体クロマトグラフィーによる研究 第3編 ラットのm-, p-Xylene代謝に及ぼすPhenobarbitalの影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In order to estimate the influence of phenobarbital on the metabolism of m-, p-xylene, female Wistar rats were pretreated with intraperitoneal injection of sodium phenobarbital (75 mg per kg body weight) for 3 days (PB group). On the 4th day, 4.667 mmole per kg of m-xylene or p-xylene was injected intraperitoneally and urine samples were collected at 4 hour intervals thereafter. The urinary content of m-methylhippuric acid and p-methylhippuric acid was determined by high-performance liquid chromatography. Urinary excretion of m-methylhippuric acid or p-methylhippuric acid in the PB group was significantly higher than in the control group (pretreated with 2 ml per kg of normal saline instead of phenobarbital) at the 0-4 hour and 4-8 hour intervals (p<0.01). This suggests that oxidation of methyl groups of m- and p-xylenes can be performed by the microsomal drug metabolizing enzyme in liver. There was no significant difference between the PB and control groups in the amount of m-methylhippuric acid and p-methylhippuric acid excreted during the first 48 hours after administration.
en-copyright=
kn-copyright=

en-aut-name=SugiharaReiko
en-aut-sei=Sugihara
en-aut-mei=Reiko
kn-aut-name=杉原黎子
kn-aut-sei=杉原
kn-aut-mei=黎子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=m-キシレン
kn-keyword=m-キシレン
en-keyword=p-キシレン
kn-keyword=p-キシレン
en-keyword=フェノバルビタール
kn-keyword=フェノバルビタール
en-keyword=尿中代謝産物
kn-keyword=尿中代謝産物
en-keyword=薬物代謝酵素
kn-keyword=薬物代謝酵素
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1433
end-page=1440
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=High-performance liquid chromatographic studies of toluene and xylene poisoning Part II: Respiratory and urinary excretion of toluene and m-xylene following intraperitoneal administration to rats
kn-title=Toluene及びXyleneの中毒に関する高速液体クロマトグラフィーによる研究 第2編 ラットにおけるToluene及びm-Xyleneの排泄
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The purpose of this study is to investigate differences in excretion of toluene and m-xylene. After intraperitoneal injection of toluene or m-xylene (5 mmole per kg body weight) to Wistar female rats, urine and expired air were collected regularly. The concentrations of toluene and m-xylene in expired air were determined spectrophotometrically. Their urinary metabolites hippuric acid and m-methylhippuric acid were measured by high-performance liquid chromatography. The results were: 1. The excretion rates in expired air for 10 hours after the administration of toluene and m-xylene were 37.7 % and 13.1 % respectively. After the injection of toluene, the excretion rate per hour of the vapor in expired air increased rapidly, and reached a maximum (6.1 % ) in 2 hours, then decreased in a half-life of 4.87 hours. The maximum excretion rate of m-xylene per hour was 1.6 % in 3 or 4 hours; thereafter, the excretion rate decreased more slowly than in the case of toluene. 2. The total excretion rate in urine for 48 hours after administration was 29.0 % for toluene and 43.5 % for m-xylene. Urinary excretion of m-xylene is apparently higher than that of toluene, in contrast to expiratory excretion. Following the administration of toluene, about 14 % had been eliminated as hippuric acid in the 0-10 and 10-24 hours' urine. The concentration of hippuric acid had returned to normal in the 24-48 hours' urine. m-Methylhippuric acid in the 0-10 hours' urine was 13.7 % of m-xylene; in the 10-24 hours' urine it was 22.3 % ; moreover, 7.5 % was found even in the 24-48 hours' urine. From these results, it is estimated that the velocity of urinary excretion of m-xylene is less than that of toluene. 3. The total excretion rate of toluene in urine and expired air was 51.9 % and that of m-xylene was 26.9 % , for 10 hours after the administration. Furthermore, the excretion rate of both 10 hours' expired air and 48 hours' urine, was 66.7 % for toluene and 56.6 % for m-xylene. 4. The ratio of 48 hours' urinary excretion to 10 hours' expiratory excretion was 0.77 for toluene and 3.58 for m-xylene. These results indicate that toluene was excreted more in expired air than in urine whereas m-xylene was mainly excreted in urine.
en-copyright=
kn-copyright=

en-aut-name=SugiharaReiko
en-aut-sei=Sugihara
en-aut-mei=Reiko
kn-aut-name=杉原黎子
kn-aut-sei=杉原
kn-aut-mei=黎子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=トルエン
kn-keyword=トルエン
en-keyword=m-キシレン
kn-keyword=m-キシレン
en-keyword=呼気
kn-keyword=呼気
en-keyword=尿中代謝産物
kn-keyword=尿中代謝産物
en-keyword=排泄率
kn-keyword=排泄率
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1427
end-page=1432
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=High-performance liquid chromatographic studies of toluene and xylene poisoning Part I: Workers' exposure to toluene and xylenes estimated from metabolites in their urine in a shipyard
kn-title=Toluene及びXyleneの中毒に関する高速液体クロマトグラフィーによる研究 第1編 尿中代謝産物よりみた某造船所におけるToluene, Xylene暴露の実態
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=In this paper, the author describes the determination of metabolites in the urine of shipyard workers exposed to thinner containing toluene and xylenes. Urine was taken from 4 healthy workers working at the same place, in the morning (a.m. 8:00), at noon (p.m. 0:00) and in the afternoon (p.m. 5:00), 3 times in a day for 5 consecutive days. Urinary metabolites, i.e., m-methylhippuric acid, p-methylhippuric acid and hippuric acid, were determined by high-performance liquid chromatography. The results were: 1. m-Methylhippuric acid, p-methylhippuric acid and hippuric acid in the urine of painting workers could be determined by high-performance liquid chromatography following extraction and benzoxylation. The stationary phase used in this experiment was silica gel and the mobile phase was a mixture of n-hexane and chloroform. 2. Concentrations of urinary m- and p-methylhippuric acids and hippuric acid were lowest just before, and generally highest just after, work. These results suggest that the workers were inhaling the thinner vapor even though wearing gas masks. 3. Peaks of m- and p-methylhippuric acids occurred in the chromatogram of workers' urine collected before work in the morning, although there were no peaks in the chromatogram of normal urine. This indicates that m- and p-xylenes may still remain in the workers' bodies.
en-copyright=
kn-copyright=

en-aut-name=SugiharaReiko
en-aut-sei=Sugihara
en-aut-mei=Reiko
kn-aut-name=杉原黎子
kn-aut-sei=杉原
kn-aut-mei=黎子
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部公衆衛生学教室
en-keyword=シンナー
kn-keyword=シンナー
en-keyword=キシレン
kn-keyword=キシレン
en-keyword=m-メチル馬尿酸
kn-keyword=m-メチル馬尿酸
en-keyword=p-メチル馬尿酸
kn-keyword=p-メチル馬尿酸
en-keyword=高速液体クロマトグラフィー
kn-keyword=高速液体クロマトグラフィー
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1389
end-page=1426
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A scanning electron microscopic study of the microcirculation of the esophagus A vascular cast observation
kn-title=血管鋳型法による食道壁微細血管構築の走査電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The fine distribution of blood vessels of the esophagus of rat, rabbit, and man was studied by the corrosion cast method. The vascular casts were made by injecting Mercox into the artery of the esophagus and removing the tissue with 20% KOH. These were observed under a scanning electron microscope. In addition, the healing process of an anastomosing part in the lower portion of the esophagus of rat was observed by the same method. In each layer of the esophagus particular microvascular patterns were observed as follows: 1) At the tunica adventitia thin capillaries formed a coarse meshwork, and large vessels ran through this capillary mesh in places. Capillary nets of muscle layers were thick and dense. These were composed of two layers which corresponded to the longitudinal and circular muscle layers. 2) Arteries of the submucosa ran longitudinally, and after branching or anastomosing, formed fine networks in the submucous layer. Numerous veins which pierced the muscularis mucosa from the lamina propria increased in diameter and also ran longitudinally in the submucous layer. They were connected by numerous cross anastomoses and some perforated the muscle coats of the esophagus to reach the outer surface. These vessels in the submucous layer constituted the submucous plexus. 3) At the lamina propria small arteries coming from the submucosa branched out and finally divided into capillaries. These capillaries formed a coarse and slightly elongated polygonal meshwork. In man the capillary mesh of the lamina propria projected into the lumen of the esophagus and showed a similar appearance to the vascular pattern of the villi of the small intestine. Small veins receiving their blood supply from the capillary mesh and connecting with numerous cross anastomoses ran longitudinally in the lamina propria and finally joined into the veins of the submucosa. 4) Microvascular patterns at each stage of the healing of an anastomosis of the esophagus were observed as follows. i) 3rd day postoperation: Vascular casts were lacking at the site of anastomosis. By observation with high magnification the buds of regenerating vessels were found at the wall of large vessels of the anastomosing site. ii) 5th day postoperation: The regenerating vessels had elongated, and anastomosis with vessels of the opposite side had begun. iii) 7th day postoperation: Revascularisation at the anastomosing part was
almost completed. The microvascular pattern of each layer still remained irregular and showed incompletion of the regeneration of blood vessels. iv) 14th day postoperation: As the constitution of blood vessels of each layer of the anastomosing part showed almost normal appearance it was assumed that the healing of an anastomosis of the esophagus had been mostly completed by this stage.
en-copyright=
kn-copyright=

en-aut-name=KinoshitaShoichi
en-aut-sei=Kinoshita
en-aut-mei=Shoichi
kn-aut-name=木下詔一
kn-aut-sei=木下
kn-aut-mei=詔一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
en-keyword=血管鋳型法
kn-keyword=血管鋳型法
en-keyword=食道壁微細血管構築
kn-keyword=食道壁微細血管構築
en-keyword=食道吻合
kn-keyword=食道吻合
en-keyword=血管新生
kn-keyword=血管新生
en-keyword=創癒合
kn-keyword=創癒合
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1369
end-page=1388
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Comparative anatomic and electron microscopic study of specific granules of heart atrial muscle
kn-title=心房筋特殊顆粒に関する比較解剖学的,電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Specific granules of heart atrial muscle obtained from mammals (mouse, rat, rabbit, dog, pig, ox and monkey), aves (chicken), reptiles (Geoclemmys), amphibia (Rana), pisces (carp, goldfish and shark) and cyclostomata (Entosphenus) were investigated by electron microscope and the following results were obtained. The mean diameter of the specific granules was large in rat (315 nm) and mouse (283 nm), medium in rabbit (260 nm) and pig (210 nm) and small in dog and ox (170 nm) and monkey (175 nm) among mammals. The core of these granules was electron-translucent in rat and mouse and electron-dense in rabbit. The atria of rat and mouse had a large number of granules while that of monkey showed a fairly large number of granules. The dog showed a small number of granules and the granules of ungulate (pig and ox) were sparse. Granules in vertebrates other than mammals were relatively smaller than in mammals. The mean diameter was 144 nm in chicken, 175 nm in Geoclemmys, 150 nm in Rana and carp and 168 nm in Entosphenus. The atria of Geoclemmys and Rana had a large number of granules while that of Entosphenus also had a fairly large number of granules. Shark only showed a small number of granules and the granules of chicken, carp and goldfish were sparse. Electron microscopic study of specific granules in rat heart atrial muscle showed that there were two types of granules. One (Type I) had a homogenous and electron-dense core and the other (Type II) was electron-translucent and consisted of small granular materials. Both were enclosed by a limiting membrane. Type II were distributed near the Golgi apparatus present at the perinuclear area of the muscle cell while Type I were widely distributed in sarcoplasm at the perinuclear area, between myofibrils and beneath the cell membrane of the cell. From the results, the process of the formation, maturation and release of specific granules of heart atrial muscle was discussed.
en-copyright=
kn-copyright=

en-aut-name=KishimotoToshihiko
en-aut-sei=Kishimoto
en-aut-mei=Toshihiko
kn-aut-name=岸本俊彦
kn-aut-sei=岸本
kn-aut-mei=俊彦
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一解剖学教室
en-keyword=脊椎動物―心房筋細胞―特殊顆粒―
kn-keyword=脊椎動物―心房筋細胞―特殊顆粒―
en-keyword=I型とII型顆粒―開口分泌
kn-keyword=I型とII型顆粒―開口分泌
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1353
end-page=1367
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on histochemical methods for observation of glycogen
kn-title=グリコーゲンの検索のための組織化学的方法に関する検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The effects of six fixatives (Carnoy, Allen, Gendre, Rossman, Glutaraldehyde and Periodic acid-formalin) on the distribution of glycogen of the liver were studied by light and electron microscope. In tissue fixed with Carnoy, Allen, Gendre and Rossman, liver cells showed an alcohol flight phenomenon and chess board pattern was observed in the center of the tissue. The results obtained from freeze-drying method, that is, the alcohol flight phenomenon was an artifact, but the chess board pattern was not. In tissue fixed with glutaraldehyde and periodic acid-formalin, the alcohol flight phenomenon was rarely observed but the chess board pattern was clearly recognized. To study the effect of the temperature of fixatives on the changes in glycogen distribution, liver was fixed with glutaraldehyde and periodic acid-formalin at various temperatures. Good results were obtained with periodic acid-formalin under 20℃ or with glutaraldehyde under 4℃. Electron microscopy showed that five fixatives (except glutaraldehyde) caused cell disruption and fine ultrastructure was not observed. Glycogen was observed as β and α particles with glutaraldehyde fixation. The effect of pH of glutaraldehyde solution on glycogen distribution was not discernible. These results showed that fixation with glutaraldehyde was the best method to study glycogen particles by both light and electron microscope.
en-copyright=
kn-copyright=

en-aut-name=NagitaTakashi
en-aut-sei=Nagita
en-aut-mei=Takashi
kn-aut-name=名木田隆史
kn-aut-sei=名木田
kn-aut-mei=隆史
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一解剖学教室
en-keyword=グリコーゲン→組織化学―
kn-keyword=グリコーゲン→組織化学―
en-keyword=固定液-温度―pH
kn-keyword=固定液-温度―pH
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1333
end-page=1351
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Effects of coronary vasoactive drugs on coronary collateral circulation in anesthetized open-chest dogs (An experimental study on coronary collateral circulation by measurement of retrograde coronary blood flow)
kn-title=冠血管作働薬の冠副行循環に及ぼす影響(逆行性血流量測定法による実験的研究)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The effects of coronary vasoactive drugs on coronary collateral were studied in anesthetized open-chest dogs. The circumflex coronary artery was gradually occluded by an aneroid constrictor. Four to eight weeks later, retrograde coronary blood flow from the peripheral coronary artery and other parameters were studied. The results were as follows. 1) Propranolol (0.2 mg/kg) decreased myocardial oxygen consumption represented as pressure rate product and increased peripheral coronary vascular resistance slightly. Retrograde coronary blood flow decreased significantly. The effect of propranolol was to improve the imbalance between myocardial oxygen demand and supply. 2) Nitroglycerin (20 μg/kg) caused a reduction in preload and myocardial oxygen consumption. Retrograde coronary blood flow did not increase. 3) Diltiazem (0.15 mg/kg) decreased myocardial oxygen consumption. Retrograde coronary blood flow was not significantly changed. 4) Isoproterenol (0.2 μg/kg) aggravated the myocardial oxygen demand-supply relationship. 5) There was a significant reduction in the regional myocardial shortening in the ischemic area compared with the control area. The response of regional myocardial shortening to the drugs in the ischemic area, however, was similar to that in the control area.
en-copyright=
kn-copyright=

en-aut-name=OginoYasuhiro
en-aut-sei=Ogino
en-aut-mei=Yasuhiro
kn-aut-name=荻野泰洋
kn-aut-sei=荻野
kn-aut-mei=泰洋
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
en-keyword=retrograde coronary blood flow
kn-keyword=retrograde coronary blood flow
en-keyword=ameroid constrictor
kn-keyword=ameroid constrictor
en-keyword=coronary vasoactive drugs
kn-keyword=coronary vasoactive drugs
en-keyword=double product
kn-keyword=double product
en-keyword=coronary collateral development
kn-keyword=coronary collateral development
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1317
end-page=1332
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental study of safety limits in differential hypothermia Part 2. Metabolic effects on normal rabbit brains
kn-title=Differential Hypothermia処置の安全限界に関する実験的研究 第2編 家兎正常脳の代謝への影響
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Differential hypothermia (D.H.) treatment was performed on normal rabbit brains and the associated changes in cerebral metabolism were observed in series. Under generalized body hypothermia (rectal temperature; 23℃), a portion of normal brain was maintained at normothermia (37±1℃) by heating with microwave irradiation (2.45GHz). Brain tissues were sampled as follows; before treatment, at the beginning, 3, 6 and 10 hours later, and finally 12 and 36 hours after completion of the 10 hour-treatment. Five rabbits were studied in each group to be 35 as a total sum. In each rabbit, two samples; one from the normothermic portion and one from the hypothermic portion, were obtained. By using enzymatic analysis, six glycolytic metabolites (glucose, glucose-6-phosphate, fructose-1,6-diphosphate, dihydroxyacetone phosphate, pyruvate, lactate) and three adenine nucleotides (ATP, ADP, AMP) were investigated quantitatively. From these results, the ratio of lactate and pyruvate (L/P ratio) and energy charge potential (ECP) were calculated. Before the treatment, the L/P ratio was 26.4 and ECP was 0.921. Up to 6 hours after the beginning, the metabolic status in the normothermic portion was relatively higher than the hypothermic portion. Namely, L/P ratio and ECP in the normothermic portion reached 27.6 and 0.953 respectively. On the completion of the 10 hour-treatment, however, glucose decreased considerably. While G6P increased markedly associated with a L/P ratio of 47.5. Similarly ATP was decreased, while ADP and AMP were increased, therefore ECP decreased to 0.873. These data indicated tissue hypoxia. On the other hand, 36 hours after the treatment, the L/P ratio recovered completely to 18.7, ECP also recovered to 0.901. These results indicated that metabolism in the normothermic portion was smooth up to 6 hours duration of treatrent, although it was constantly higher than the hypothermic portion. On the completion of the 10 hour-treatment, increase of glycolysis by tissue hypoxia and failure of energy metabolism were observed. However, these changes were considered still reversible because the results obtained after the treatment indicated reasonable recovery. Therefore it was suggested that 10 hour-differential hypothermia treatment on the normal rabbit brains was safe from a viewpoint of glycolytic and energy metabolism.
en-copyright=
kn-copyright=

en-aut-name=IkedaKomei
en-aut-sei=Ikeda
en-aut-mei=Komei
kn-aut-name=池田幸明
kn-aut-sei=池田
kn-aut-mei=幸明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学脳神経外科学教室
en-keyword=differential hypothermia
kn-keyword=differential hypothermia
en-keyword=normal brain
kn-keyword=normal brain
en-keyword=glucose
kn-keyword=glucose
en-keyword=adenine nucleotides
kn-keyword=adenine nucleotides
en-keyword=metabolic changes
kn-keyword=metabolic changes
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1301
end-page=1316
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental study of safety limits in differential hypothermia Part 1. Heating of brain by microwave irradiation
kn-title=Differential Hypothermia処置の安全限界に関する実験的研究 第1編Microwave照射による脳加温に関して
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Differential hypothermia (D.H.) treatment (preservation of brain tumor at normothermia during generalized body hypothermia) for the treatment of human brain tumors requires the tumor to be heated throughly and maintained for the necessary time. The normal portion of brain has to be protected during and after the treatment. Microwave (MW) seemed the most suitable heating method, so its effects on the normal brain of dogs were studied. During MW irradiation, the thermistor-probe showed higher temperatures erroneously. When the probe was covered by aluminium foil, the measurement error was less than 0.2℃ as long as the probe was in the brain. This method sufficiently heated the brain under generalized hypothermia down to 2 cm in depth from the surface. The highest temperature was recorded between 0.5 cm and 1 cm in depth and was easily controlled by the output of MW. On the other hand, aluminium foil blocked MW irradiation completely, therefore the irradiation field possibly protected the normal portion. To study the safety limits of heating by MW irradiation, two groups of rabbits maintained at different temperatures (37℃ and 40℃) were prepared. Following D.H. treatment for 5 hours in each group, the rabbit brains were examined histologically. The results showed minimal brain damage by MW irradiation in the 37℃ group. In the 40℃ group, heat necrosis was constantly observed as well as stagnation of vessels and perivascular cuffing of round cells. From these findings, it was concluded that MW was useful as a heating method during D.H. treatment. The safety limit of heating was up to normothermia.
en-copyright=
kn-copyright=

en-aut-name=IkedaKomei
en-aut-sei=Ikeda
en-aut-mei=Komei
kn-aut-name=池田幸明
kn-aut-sei=池田
kn-aut-mei=幸明
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学脳神経外科学教室
en-keyword=differential hypothermia
kn-keyword=differential hypothermia
en-keyword=microwave irradiation
kn-keyword=microwave irradiation
en-keyword=normal brain
kn-keyword=normal brain
en-keyword=histopathology
kn-keyword=histopathology
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1269
end-page=1299
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Scanning electron microscope study of rat thymus
kn-title=ラット胸腺における走査型電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Scanning electron microscope observations were conducted on cross-sections and bloodvessel casts of normal rat thymus. A comparative study was carried out with thymus crosssections prepared from rats injected with corticosteroid and from rats undergoing muscleembedding transplantation. The results were as follows: Cortical lymphocytes of normal rat thymus had fewer microvilli, while medullary lymphocytes had many. The network made by epithelial cells was smaller but that made by the medullary cells was larger. The cortex medulla boundary was distinct on the cortical side and indistinct on the medullary side. The interior surface structures of the posterior capillary venules presented a variety of changes which enabled smooth wandering of the cells. There were also some lymphocytes penetrating through the vascular endothelium. With blood vessel casts cicatrization of the posterior capillary venules was characteristic. On the interior surface of the thymus cyst there were villous or cribriform structures. In the cortex of the rat thymus injected with corticosteroid destruction of lymphocytes was marked, and in the medulla a decrease in the number of lymphocytes was the main change. In the rat thymus after muscle-embedding transplantation cortical lymphocytes appeared rounded and there were many ciliated cells.
en-copyright=
kn-copyright=

en-aut-name=YoshiiYasunori
en-aut-sei=Yoshii
en-aut-mei=Yasunori
kn-aut-name=吉井康哲
kn-aut-sei=吉井
kn-aut-mei=康哲
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
en-keyword=ラット胸線
kn-keyword=ラット胸線
en-keyword=走査電子顕微鏡
kn-keyword=走査電子顕微鏡
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1259
end-page=1268
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Hemolytic anemia in Rauscher virus-induced leukemia 2. Studies on function of reticuloendothelial system
kn-title=Rauscher Virus性マウス白血病における溶血性貧血に関する研究 第2編 網内系機能の変動について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Using Rauscher leukemia virus, changes in the reticuloendothelial phagocytic activity were studied by a carbon clearance method and a heat-damaged (51)Cr-labelled red blood cell method. With the carbon clearance method, after virus infection, phagocytic activity was slightly increased in the early stages, but decreased gradually during later stages. With the (51)Cr-labelled RBC method, in the early stages, phagocytic activity of liver and spleen was slightly increased and during the later stages accelerated markedly in the liver in contrast to supressed activity in the spleen. It was suggested that an early increase of phagocytic activity observed by both methods was a response to viral infection. Significantly accelerated phagocytic activity seen in the liver during later stages with the (51)Cr method may have been related to the hemolytic anemia associated with Rauscher leukemia.
en-copyright=
kn-copyright=

en-aut-name=MizukawaIchiro
en-aut-sei=Mizukawa
en-aut-mei=Ichiro
kn-aut-name=水川市郎
kn-aut-sei=水川
kn-aut-mei=市郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科教室
en-keyword=溶血性貧血
kn-keyword=溶血性貧血
en-keyword=網内系貪食機能
kn-keyword=網内系貪食機能
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1249
end-page=1258
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Hemolytic anemia in Rauscher virus-induced leukemia 1. Studies on hematology, histology, red cell survival and ferrokinetics
kn-title=Rauscher virus性マウス白血病における溶血性貧血に関する研究 第1編 血液,組織学的検索と赤血球寿命及び鉄代謝について
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Hemolytic anemia was associated with Rauscher leukemia. Anemia was observed slightly in the early stages of leukemia and developed progressively during the later stages. The mechanism of hemolytic anemia can be explained as being due to both intracorpuscular defects and extracorpuscular factors. The former, i.e., intracorpuscular defect, consists of intracorpuscular defects caused by direct effects of Rauscher leukemia virus on red blood cell and also "abnormal" erythrocytes which migrate into peripheral blood from the spleen. These "abnormal" erythrocytes were produced by the maturation of malignant erythoid cells induced by Rauscher leukemia virus. The latter, i.e., extracorpuscular factor, will be described part two of this article.
en-copyright=
kn-copyright=

en-aut-name=MizukawaIchiro
en-aut-sei=Mizukawa
en-aut-mei=Ichiro
kn-aut-name=水川市郎
kn-aut-sei=水川
kn-aut-mei=市郎
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科教室
en-keyword=溶血性貧血
kn-keyword=溶血性貧血
en-keyword=実験的virus性白血病
kn-keyword=実験的virus性白血病
en-keyword=赤血球寿命
kn-keyword=赤血球寿命
en-keyword=鉄代謝
kn-keyword=鉄代謝
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1235
end-page=1248
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Significance of persistent juvenile T wave patterns
kn-title=健常成人の若年性T波(Persistent Juvenile T Wave Pattern)に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Persistent juvenile T wave patterns found in mass examination and in clinical cases were studied for the purpose of clarifying their significance. Persistent juvenile T wave patterns were classified into three types. Type I had negative T waves in lead V(1) only, Type II in lead V(1) and V(2) and Type III in V(1) to lead V(3) or V(4). During mass examinations, persistent juvenile T wave patterns were found in 243 cases (43.5%) of 558 male subjects. Of these, 15 (6.2%) were Type II, III. On the other hand they occurred in 547 (66.5%) of 823 female subjects. Of these, 86 (15.7%) were Type II, III. The incidence of each Type of persistent juvenile T wave pattern did not vary significantly with age, but Type II, III were relatively more frequent between 35 and 49 years of age. Persistnet juvenile T wave patterns were often positive in many cases in electrocardiograms (ECG) thaken from the same subjects 8 years after the first mass examination. Standard ECG's and Frank's system vectorcardiogram (VCG)'s were analysed in 116 clinical cases showing persistent juvenile T wave patterns. Transitional zone and electrical axis deviation in ECGs were almost normal in the persistent juvenile T wave pattern. The shape of the transverse T loop was mostly normal whereas the transverse QRS loop normally varied in shape. Clockwise rotation of the transverse T loop was observed in 15 of 116 clinical cases. The maximum magnitude of the T loop in VCG was significantly smaller in Type III than in Type I, II. Changes in the persistent juvenile T wave pattern on ECG and VCG were studied in 25 cases after administration of Propranolol. The negative T wave in ECG tended to become positive and the maximal T vector tended to become larger shifting towards anterior in VCG after administration of Propranolol. The persistent juvenile T wave pattern was observed more frequently in middle aged women and usually became normal after administration of Propranolol. These results suggest that increased sympathetic tone is one of the pathological mechanisms involoved in the persistent juvenile T wave pattern.
en-copyright=
kn-copyright=

en-aut-name=TakaharaMitsuhiro
en-aut-sei=Takahara
en-aut-mei=Mitsuhiro
kn-aut-name=高原光弘
kn-aut-sei=高原
kn-aut-mei=光弘
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
en-keyword=若年性T波
kn-keyword=若年性T波
en-keyword=T波およびT環
kn-keyword=T波およびT環
en-keyword=交感神経緊張
kn-keyword=交感神経緊張
en-keyword=β遮断剤
kn-keyword=β遮断剤
en-keyword=集団検診
kn-keyword=集団検診
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1207
end-page=1234
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Cardiac sympathetic nerve stimulation and electrocardiographic changes in the closed-chest dog
kn-title=心臓交感神経刺激による心電図変化(実験的検討)
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Electrocardiographic changes caused by electrical stimulation of individual cardiac sym-pathetic nerves were observed with Frank's corrected orthogonal lead system and Frank's vetorcardiogram. The effects of drugs on nervous stimulation and the electrocardiographic changes after the resection of the cardiac nerves were investigated in 45 closed chest dogs. The following results were obtained: 1. The amplitude of the Twave decreased in Y lead and increased in Z lead in 20 out of 34 cases in which the right stellate ganglion [R.S.G.(A)] had been stimulated and in all cases in which the right recurrent cardiac nerve (R.Rec.C.N.) and the left ventromedial cervical cardiac nerve (L.V.M.C.N.) had been stimulated. Spatial maximum T vector displaced anteriorly and superiorly. The magnitude of spatial maximum T vector increased in all cases. 2. An increase in the amplitude of T waves in Y lead was seen in all cases in which the left stellate ganglion (L.S.G.), and the left ventrolateral cervical cardiac nerve (L.V.L.C.N.) had been stimulated, and in 14 out of 34 cases in which the right stellate ganglion [R.S.G.(B)] had been stimulated. The spatial maximum T vector was displaced inferiorly. The magnitude of the spatial maximum T vector increased in all cases. 3. The amplitude of the T wave increased in Y lead with simultaneous stimulation of both right and left stellate ganglia. The spatial maximum T vector was displaced inferiorly and the magnitude of the spatial maximum T vector increased in all cases. These electrocardiographic changes were similar to the changes which occurred with single L.S.G. stimulation. 4. Rotation of the T loop in the left saggital plane was clockwise in more than 80 % of cases with R.S.G. stimu-lation. However, rotation of the T loop in left saggital plane was counter clockwise in more than 75-80% of cases with L.S.G. and simultaneous stimulation of both right and left stellate ganglia. These results suggest that right and left cardiac sympathetic nerves innervate different myocardial areas and affect the local myocardial action potentials to cause these electrocardiographic changes. 5. In more than 40% of cases with stimulation of left cardiac sympathetic nerves (L.S.G., L.V.L.C.N.). arrythmias such as A-V dissociation and A-V junc-tional rythm were recognized. In more than 90% of cases with stimulation of right cardiac sympathetic nerves (R.S.G., R.Rec.C.N.), marked increase of sinus rythm occurred. 6. These electrocardiographic changes resulting from nervous stimulation decreased or disappeared after the use of propranolol. These results suggest that these electrocardiographic changes were caused by β -effects of catecholamines. 7. The QT ratio corrected by the formula of Bazett increased in all cases of right and left cardiac sympathetic nervous stimulation. 8. Long term observations after right or left stellate ganglionectomy revealed no significant changes in electrocardiographic patterns and a tendency to decrease in the QT ratio corrected by the formula of Bazett.
en-copyright=
kn-copyright=

en-aut-name=YoshidaHidenori
en-aut-sei=Yoshida
en-aut-mei=Hidenori
kn-aut-name=吉田英紀
kn-aut-sei=吉田
kn-aut-mei=英紀
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一内科教室
en-keyword=stellate ganglion T wave QT interval
kn-keyword=stellate ganglion T wave QT interval
en-keyword=catecholamine action potential
kn-keyword=catecholamine action potential
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1171
end-page=1206
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A scanning electron microscopic study on regenerating rat liver sinusoid
kn-title=ラット再生肝における血管の走査電子顕微鏡的研究-特に類洞における変化-
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=With rats from which about 70% of the liver was previously removed, corrosion casts and specimens of freeze-cracking method were prepared from time to time after the operation. These specimens were used for scanning electron microscope observation. Ultra-thin sections were also prepared for transmission electron microscope observation to study regeneration of the sinusoid during regeneration of the liver. The results were compared with normal rat liver, and were as follows: Regenerative proliferation of the sinusoid after partial hepatectomy began about 12 hours after hepatectomy. First the space of Disse enlarged. This later becomes a sinusoid, so the author had designated it as a presinusoid space. The presinusoid spaces appear about 12 hours after hepatectomy, and can be observed up to about the third post- operative day. In the early stages, they are spherical, about 2-10 μ in diameter, and grow larger with lapse of time. Later they become larger than the sinusoid. Some have many bumps running parallel to the sinusoids, while others bind with the sinusoid to form bridges. They are located at the margin of the sinusoid facing one fixed direction. The sinusoid and the presinusoid space are separated by endothelial cells lying in between them. The other external wall is composed of liver cells. The number of microvilli of the liver cell surface is extremely small. This stage has been called the stage of the presinusoid space. In the presinusoid space, the liver cell surface which forms the exterior wall shows newly formed endothelial cells after cell division. These protrude and completely cover the inner surface. At the same time a neoplastic sinusoid forms by removing the endothelial cells there. The formation of the new sinusoid starts about 24 hours after partial hepatectomy and continues up to about the third postoperative day. This stage has been designated the sinusoid budding stage. The neoplastic sinusoid is at first closed at one end but later these neoplastic sinusoids fuse to form a new sinusoidal network. Such fusion commences about 48 hours after partial hepatectomy, and continues vigorously for about 3 postoperative days. This stage is called the fusion stage. The new sinusoidal network formed by the fusion of neoplastic sinusoids is irregular, and its surface is uneven, but with time, the arrangement and the surface contour become regular and smooth. The process is completed by about 14 postoperative days. This stage has been designated the completion stage.
en-copyright=
kn-copyright=

en-aut-name=YamadaSeiichi
en-aut-sei=Yamada
en-aut-mei=Seiichi
kn-aut-name=山田征一
kn-aut-sei=山田
kn-aut-mei=征一
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科教室
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1139
end-page=1170
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=A scanning electron microscopic study on the microcirculating of the rat adrenal gland
kn-title=ラット副腎の血管微細構築に関する走査電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The microcirculation of the rat adrenal gland was studied with a corrosion cast technique and a freeze-cracking method under scanning electron microscopy. 1) The adrenal arteries run in the surface of the adrenal gland, branch off into arterioles which finally form the subcapsular plexus. This plexus is a dense anastomosing network covering the surface of the adrenal gland. 2) The cortical capillaries arise from the subcapsular plexus, and run vertically towards the adrenal medulla. These capillaries have sinusoidal structures. 3) The cortical capillaries make up a honey-comb pattern in the outer glomerulosal zone and become dense and thick in the cortico-medillary border. 4) The medullary, cortical and looped arteries penetrate the subcapsular plexus and run vertically into the cortex. 5) Most medullary arteries have 1 to 3 branched which run back towards the surface to join in the subcapsular plexus. Hence the author termed these recurrent arteries. The medullary artery occasionally has a branch which runs to the medulla, but does not anastomose with the cortical capillary plexus. 6) The capillaries of the medullary artery are smaller, and the plexus which is made of these capillaries is more coarse than the cortical plexus. These plexes have a coarse anastomosis with cortical capillaries and then run into the medullary vein. 7) The medullary veins drain into one adrenal vein. The adrenal vein anastomoses with the subcapsular plexus in rats as well. 8) Similar results were observed in the freeze-cracked specimen. In addition, in the endothelium of the cracked cortex, many polymorphous fenestrations were observed which were analogous to liver sinusoidal fenestrations. 9) The shape and size of the fenestrations vary with the technique of making the specimen. The fenestrations were very small and uniform when the adrenal gland was not irrigated.
en-copyright=
kn-copyright=

en-aut-name=NishiyamaMasao
en-aut-sei=Nishiyama
en-aut-mei=Masao
kn-aut-name=西山正夫
kn-aut-sei=西山
kn-aut-mei=正夫
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第一外科学教室
en-keyword=副腎の血管微細構築
kn-keyword=副腎の血管微細構築
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1127
end-page=1137
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Inhibitory effect of cephalanthin on radiation-induced haemolysis of human erythrocytes
kn-title=放射線照射による人赤血球の溶血と“セファランチン”による阻止作用
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=X-radiation decreases the number of RBC in vivo and accelerates hemolysis in vitro, but the mechanism is still unclear. Taking the view that these phenomena are due to radical formation and active enzyme formation followed by accelerated peroxidation of cell membrane lipid and membrane structural changes we studied the morphological changes of RBC, changes in ion-partition, and the inhibitory effects of drugs. The results were as follows: 1) Hemolysis of human erythrocytes (RBC) was accelerated by x-radiation. The degree of such lysis was proportional to the degree of x-radiation. This lysis was inhibited markedly by the addition of cephalanthin. The inhibitory effect was dependent on the concentration. In high concentrations conversely acceleration occurred. 2) Soon after x-radiation and prior to hemolysis, human RBCs released K(+) bringing about a decrease in the K(+) partition. The time of this decrease paralleled the degree of x-radiation. The K(+)-partition decrease caused by x-radiation was inhibited by the addition of cephalanthin. 3) In parallel with the acceleration of erythrocyte membrane permeability by x-radiation, changes in RBC morphology were more likely, thus causing structural changes in the cell membrane. 4) Cephalanthin protects the RBC from its instability to x-radiation, and at certain concentrations it restores normal morphology to RBC that have an Echinocyte structure, but at high concentrations it makes RBC to assume a stomatocyte shape. 5) The above findings are discussed in terms of cell membrane injury mechanisms and the inhibitory effect of cephalanthin.
en-copyright=
kn-copyright=

en-aut-name=IidaSosuke
en-aut-sei=Iida
en-aut-mei=Sosuke
kn-aut-name=飯田荘介
kn-aut-sei=飯田
kn-aut-mei=荘介
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部放射線医学教室
en-keyword=アルカロイド
kn-keyword=アルカロイド
en-keyword=放射線照射
kn-keyword=放射線照射
en-keyword=溶血
kn-keyword=溶血
en-keyword=人赤血球
kn-keyword=人赤血球
en-keyword=形態変化
kn-keyword=形態変化
en-keyword=細胞膜K(+)区画性
kn-keyword=細胞膜K(+)区画性
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1117
end-page=1125
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells　as target cells　Part II. ADCC and erythrophagocytosis of peripheral leukocytes in patients with Sjogren's syndrome or rheumatoid arthritis
kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第2編 Sjogren症候群ならびに慢性関節リウマチ患者白血球のADCC活性とErythrophagocytosis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent erythrophagocytosis (ADEP) of peripheral blood leukocytes in patients with Sjogren's syndrome (SjS) or rheumatoid arthritis (RA) were investigated using (51)Cr-labelled sheep red blood cells as target cells. The Lymphocytes (phagocyte depleted fraction, PDF) in healthy controls showed higher ADCC activities (58.6±9.9%) than the phagocyte-contaminated crude lymphocyte fraction (CLF) (49.1±10.9%). There was no statistically significant difference between the ADCC activities of PDF in patients with SjS (55.9±13.5%) or RA (58.8±9.6%) and those in healthy controls, although decreased ADCC activity was observed in some patients with SjS. ADCC activities of CLF in patients with SjS (61.2±14.3%) or RA (59.3±6.9%) were significantly higher than those of CLF in healthy controls. Decreased ADEP activity of monocytes in CLF was demonstrated in patients with SjS (17.9±9.4%) compared with healthy controls (37.2±10.6%), and increased ADCC activity of CLF in patients with SjS was dependent on decreased ADEP activity of contaminating monocytes. Decreased ADEP activity of monocytes in patients with RA was also suggested by these results. It was speculated that the decreased Fc-receptor function of peripheral blood monocytes, demonstrated in these patients, had originated from aberrant cell-mediated immunity in these diseases, since these Fe-receptor functions in patients with SjS did not correeate with the following conditions: associated diseases, erythrocyte sedimentation rate, titer of serum rheumatoid factor, lymphocyte count of peripheral blood, or treatment with anti-inflammatory drugs including prednisolone.
en-copyright=
kn-copyright=

en-aut-name=IchikawaYukinobu
en-aut-sei=Ichikawa
en-aut-mei=Yukinobu
kn-aut-name=市川幸延
kn-aut-sei=市川
kn-aut-mei=幸延
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学第二内科学教室
en-keyword=antibody-dependent cell-mediated cytotoxicity
kn-keyword=antibody-dependent cell-mediated cytotoxicity
en-keyword=erthrophagocytosis
kn-keyword=erthrophagocytosis
en-keyword=sheep red blood cells
kn-keyword=sheep red blood cells
en-keyword=Sjogrens syndrome
kn-keyword=Sjogrens syndrome
en-keyword=rheumatoid arthritis
kn-keyword=rheumatoid arthritis
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1101
end-page=1115
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral blood leukocytes using sheep red blood cells as target cells Part I. Fundamental studies with special reference to erythrophagocytosis
kn-title=ヒツジ赤血球を標的細胞とするヒト末梢白血球のAntibody-Dependent Cell-Mediated Cytotoxicity (ADCC) 第1編 Erythrophagocytosisを中心とした基礎的検討
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Antibody-dependent cell-mediated cytotoxicity (ADCC) of human peripheral leukocytes was investigated using (51)Cr-labelled sheep red blood cells (SRBC) sensitized with rabbit anit-SRBC antibody as target cells. ADCC (lysis of target cells without erythrophagocytosis) was mediated by the lymphocytes. On the other hand, phagocytes including monocytes and granulocytes showed antibody-dependent erythrophagocytosis (ADEP). ADCC of the lymphocytes was augumented by the enrichment of Fc-receptor bearing or Nylon-wool column passing cells. Phagocytes showed rapid ADEP, and granulocytes released (51)Cr-radioactivity originating from target cells into the culture medium in a short time, whereas the monocytes released little radioactivity during the incubation period. The monocytes inhibited ADCC of the lymphocytes competitivelly through ADEP, since decreased ADCC activity of monocyte-contaminated crude lymphocyte fraction was recovered by elimination of contaminating monocytes. Antibody-independent erythrophagocytosis of SRBC was mediated by monocytes, but not by granulocytes. Both ADCC of lymphocytes and ADEP of phagocytes were dependent on IgG-antibody, IgM-antibody did not participate in these phenomena. ADEP of monocytes was detected at 10(-2)-10(-6) dilution of IgG-antibody fraction (original hemmagglutination titer; × 1600), and ADCC of lymphocytes and ADEP of granulocytes were detected at 10(-2)-10(-4) and 10(-2)-10(-3) dilution of this antibody fraction, respectively. Soluble cytotoxic factors were not released from lymphocytes, monocytes or granulocytes in this assay system, because no cytotoxic activity on (51)Cr-labelled human erythrocytes used as an adjacent cell was demonstrated by any leukocyte fraction in the presence of antibody-coated SRBC.
en-copyright=
kn-copyright=

en-aut-name=IchikawaYukinobu
en-aut-sei=Ichikawa
en-aut-mei=Yukinobu
kn-aut-name=市川幸延
kn-aut-sei=市川
kn-aut-mei=幸延
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第二内科学教室
en-keyword=antibody-dependent cell-mediated
kn-keyword=antibody-dependent cell-mediated
en-keyword=cytotoxicity
kn-keyword=cytotoxicity
en-keyword=erythrophagocytosis
kn-keyword=erythrophagocytosis
en-keyword=sheep red blood cells
kn-keyword=sheep red blood cells
END

start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=9-10
article-no=
start-page=1075
end-page=1099
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1979
dt-pub=19791030
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Scanning electron microscopic studies on lymphatic casts of the rat stomach and the rabbit small intestine
kn-title=胃壁および小腸壁内リンパ管鋳型に関する走査電子顕微鏡的研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Under a scanning electron microscope, the lymphatic vessels of the rat stomach and the rabbit small intestine were observed by a corrosion casting technique. The structures thus revealed were compared with those embedded in Epon by a light microscope. On the mucosal layer of the rat stomach, the lymphatic casts showed various structures, i.e., fusiform casts from the subepithelial lymphatics, a basket-shaped structure from the interglandular lymphatics and a horizontal network of leaf-like structures from the subglandular lymphatics. These casts also showed several nuclear depressions about 10 μ in diameter, a number of constrictions, and numerous small holes representing reticular or collagen fibers interrupting the Mercox filling. The submucosal lymphatic casts of the rat stomach showed irregular tubular or flat shapes about 20-40 μ in diameter, nuclear depressions about 15 μ in their long diameter with sawtoothed figures around them, bead-like swellings, and occasional V-shaped incisura on parts of the columns. Similar findings were also observed in the submucosal layer of the rabbit small intestine. On the mucosal layer of the rabbit small intestine, the central lacteal casts showed peculiar figures, i.e., a dome-shaped structure about 70-80 μ in diameter at the topmost portion, swelling to about 100-200 μ in diameter in the middle, and an evident constriction to 30-60 μ in diameter at the bottom. Some of the morphological characteristics of the central lacteal at any site of the small intestine were clarified by this study: a) in the duodenum, three or four central lacteals within one villus formed pedunclar anastomoses branching from about half way up the lamina propria, b) in the jejunum, one or two lacteals within a villus had swellings just above the bottom constriction, and c) in the ileum, each villus contained only one lacteal with a swelling in a higher position.
en-copyright=
kn-copyright=

en-aut-name=TamuraAkira
en-aut-sei=Tamura
en-aut-mei=Akira
kn-aut-name=田村章
kn-aut-sei=田村
kn-aut-mei=章
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学医学部第1外科教室
en-keyword=メルコックス樹脂
kn-keyword=メルコックス樹脂
en-keyword=胃壁内リンパ管
kn-keyword=胃壁内リンパ管
en-keyword=小腸壁内リンパ管
kn-keyword=小腸壁内リンパ管
en-keyword=リンパ管微細構築
kn-keyword=リンパ管微細構築
en-keyword=中心乳ビ腔
kn-keyword=中心乳ビ腔
END