岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 大腸菌群測定法の水質汚染指標性の検討 1309 1317 EN Kazuya Akatsuka Yasumi Mori Izumi Koujima Yoshikazu Hirai Hiroshi Hamada Masufumi Abe Shigenobu Abe Shozo Kukida Akihide Nagata Kiyoshi Fujiwara Hideo Hayashi Yasuhiro Kanemasa 水質検査には化学的検査項目以外に細菌学的検査項目が併行して行われていることは周知の事実である(1,2)).そして我国における現在の水質検査法(3))では大腸菌群測定法が広く用いられている.すなわち上水試験法(4))および工業用水(JIS)(1))ではLB培地-BGLB培地法(LB-BGLB法と略す),下水試験法ではLB培地法(LB法と略す),食品衛生法および公共用水ではBGLB培地法(BGLB法と略す)を行うとされており,これらはいずれも最確数(MPN)を求める方法である.他方乳製品および工場排水(JIS)(2))ではデスオキシコレート培地(DOC法と略す)を用いての集落数を求める方法も行われている.これら諸種の方法のうち,上水試験法および工業用水(JIS)(1))に適用されている.LB-BGLB法で算定される大腸菌群の検討を試みた.LB-BGLB法で算定される大腸菌群とは"乳糖を分解して酸とガスを産生する好気性または通性嫌気性のグラム陰性無芽胞桿菌である”.と定義され,この条件を満す種属はEscherichia,Klebsiella,Enterobacter,Aeromonas,Erwinia,SerratiaおよびCitrobacter等が包含されている.しかしこれらの種属全てが人畜糞便由来のものではなく,水,植物または土壌に由来する種属も含まれていると言うことである.元来,水質検査における汚染指標菌とは人畜糞便由来の菌を検索することを目的としているのであるから,大腸菌群測定結果をそのまま適用して汚染状況や汚染源の判定を行うと誤りを生ずることになりかねない.我々は過去数年間に亘って児島湾(5)),児島湖(6))を初めとして県下河川(7,8))での汚染調査を行って来たが,その結果のなかで化学検査や現況に照して不当と思われるような高値の大腸菌群数を算定することがしばしばあった.そこで各種調査点での現行の大腸菌群測定で得られる測定結果の検討を,本測定法で算定される大腸菌群の種属分類を行うことにより,糞便汚染の指標性の良否について検討することとした. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 放射線障害におけるスーパーオキサイド生成系(O(2))とスーパーオキサイド・ディスムターゼ(SOD)及びビタミンEの関与に対する考察 1297 1308 EN Kaname Aono Michio Yamamoto Sosuke Iida Kozo Utsumi In relation to the mechanism by which hemolysis was induced in radiated human erythrocytes in vitro, several inducements of membrane lipid peroxidation and protective effects of V.E and SOD were investigated. Results obtained were as follows: (1) K(+)-release from erythrocytes was accelerated by radiation prior to hemolysis. These accelerated hemolysis and K(+)-release were protected remarkably by V.E and evidently by SOD. (2) Mitochondrial Fe(2+) induced and Fe(3+)-O(2) generating system -ADP induced lipid peroxidation, and microsomal O(2) generating system -induced lipid peroxidation were also protected by V.E and SOD. (3) Radiation of X-ray or (60)Co γ-ray accelerated lipid peroxidation of liver homogenate, microsome and liposome. Some of these accelerated lipid peroxidations were protected effectively by V.E and SOD. These results suggest that O(2) and/or OH. generation by radiation induces of membrane lipid peroxidation, which leads deterioration of membrane resulting in the change of ion permeability and then hemolysis. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 寛解期に発症した髄膜白血病の一剖検例 1289 1295 EN Hiroshi Sonobe Kazuo Takahara Tadao Yoda Hideki Yasuda Katsuo Ogawa An autopsy of meningeal leukemia developing during hematological remission was reported. A three-year-old boy with anemia, hepatosplenomegaly and lymphadenopathy was diagnosed as having acute lymphoblastic leukemia based on hematological data. The hematological remission started about a month after the initiation of anticancerous chemotherapy with Prednisone, Vincristine and 6-Mercaptoprine. About two months after the ceasing of administration of Prednisone, symptoms such as headache and vomiting become gradually more severe. The clinical diagnosis was made as aseptic meningitis. The patient expired about two months after the onset. The autopsy revealed intense leukemic infiltration in the entire meninges and a part of the brain, but no infiltration was found in the other organs including the bone marrows, spleen, lymph nodes, liver, testis, etc., at all. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 癌の腹膜播種の成立機序に関する実験的研究 1275 1288 EN Hiroki Okabayashi The electron microscopic study of the mechanism of the implantation of the tumor cells to the peritoneum has been investigated through the rats. The experiment was made with the inoculation of Walker 256 tumor cells into their abdominal cavity. The morphalogic change and the detouching cell contact of the mesothelium make up the phenomenon, leaving empty spaces to basement membrane. This phenomenon makes it easy that the tumor cell contact with the basement membrane. This contacted tumor cell protrudes the pseudopods and begin subserosal invasion, with moving the intracellular organelles. Stretching the pseudopod, the tumor cell invades the subserosal layer, and the implantation is performed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 未完成歯再植に関する実験的研究 1249 1274 EN Shunichiro Nagahata In the field of oral surgery we apply replantation or transplantation of a tooth as one of the methods to supplement the defect of the tooth. Using puppies experimental studies were conducted on the healing process of a developing tooth after its replantation, especially on the regeneration of blood vessels in the dental pulp and periodontium that had been once depleted of its nutrient, by morphological and three-dimensional observations after the intravenous injection of chloro-percha, as well as by roentgenological and histopathological observations. The results of the observations may briefly be summarized as follows. 1) Roentgenological findings: Up to the 10th day after replantation there could be observed a spreading of pathological abnormality in the periodontal space, but after 20 days the bone regeneration could be observed, after 30 days the lamina dura appeared, and after 90 days the findings seemed to be practically normal. 2) Regenerated blood vessels in the replanted dental pulp: Up to 20 postoperative days the regenerated blood vessels increased with many fusions and windings, but after the completion of new blood flow the number of blood vessels grew less and the blood flow also became orderly, thereafter the vessels became finer with the regeneration of bony hard tissue within the dental pulp making the pulp space more narrow. 3) There could be detected no bony adhesion or absorption as observable at the replantation of a complete tooth. 4) In the fate of replanted tooth the dental pulp and periodontium play an important role. 5) There could be recognized a certain correlation in the roentgenological findings, changes of vascular pictures, and pathohistological findings. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 心房筋特殊顆粒に関する実験的・電子顕微鏡的研究 1231 1248 EN Jun Chihara Specific granules in atrial heart muscle cells were examined in normal and treated rats with the histochemical method and electron microscopy. These granules were not stained with routine staining method as azan, but when epon embedded sections were stained with toluidine blue, these granules in sarcoplasm along the nucleus were stained well. With Falck-Hillarp method, atrial muscle cells did not show any fluorescence. By the administration of 5-OHDA and 6-OHDA, false transmitters of catecholamine, the shape and the distribution of granules and the electron density of the core of granules did not change, and any fluorescence was not found with Falck-Hillarp method. The administration of 15% alchohol per os for 20 days increased the number of granules in sarcoplasm along the nucleus slightly and after 60-100 days, these granules increased also in sarcoplasm under the cell membrane or between the myofibrils. These results show that specific granules in atrial heart muscle cells do not contain catecholamine. The function of these granules is also discussed in respect to the result obtained with alchohol administration. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 Sideroblastに関する臨床的研究 第2編 癌化学療法とSideroblast 1215 1230 EN Motoharu Sugiyama The appearance of sideroblasts was investigated before and after cancer chemotherapies which were carried out 65 times in 53 patients with malignant neoplasms. After treatment, 34 cases showed marked sideroblastosis with a shift to the right in their sideroblastograms ("shifted group"). The other 31 cases showed no sideroblastosis without a shift in their sideroblastograms ("non-shifted group"). The hematological data from these two groups were then studied. After chemotherapy, the shifted group showed significant decreases in erythrocyte counts, hemoglobin concentration, leukocyte counts and nucleated cell counts and a significant increase in erythroid/myeloid ratio, whereas the non-shifted group showed no significant changes. Furthermore, the incidences of serious anemia, leukopenia, decrease in nucleated cell counts and remarkable elevation in serum iron level were significantly higher in the shifted group than in the non-shifted group. These findings suggest that marked sideroblastosis is a useful indicator in the evaluation of impaired hematopoiesis due to cancer chemotherapy. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 Sideroblastに関する臨床的研究 第1編 前白血病状態とSideroblast 1201 1214 EN Motoharu Sugiyama The appearance of sideroblasts was studied in 277 patients with various blood disorders. The appearance rate of sideroblasts was 84.1 ± 11.8% in hypoplastic anemia and 70.7 ± 23.7% in acute myelocytic leukemia, whereas it was 29.5 ± 7.8% in healthy controls. The standard sideroblastogram pattern in patients with hypoplastic anemia was type Ⅱ sideroblast dominance with moderate sideroblastosis. Three out of 87 cases of hypoplastic anemia showed type Ⅲ sideroblast dominance with conspicuous sideroblastosis. Their hematological findings were those of atypical hypoplastic anemia. One of them terminated in acute myelocytic leukemia and could be considered hypoplastic preleukemic stage retrospectively. On the other hand; the sideroblastograms of 6 out of 41 cases of acute myelocytic leukemia showed type Ⅲ sideroblast dominance. Four of them were identical with low percentage leukemia. One of these 4 cases showed ringed sideroblasts on admission and terminated in erythroleukemia at the last stage. These results suggest that in hypoplastic anemia type Ⅲ sideroblast dominance should be considered to be atypical hypoplastic anemia or hypoplastic preleukemic stage, which indicates distrurbed erythroid maturation of preleukemia. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 乳癌の発生,増殖に及ぼす甲状腺機能の影響に関する実験的ならびに臨床的研究 1179 1200 EN Nobuhiko Kaji There are controversies over the influences of thyroid function on carciogenesis and progression of the breast cancer. To reveal the interrelation of the breast cancer and thyroid function in rats, this study was performed by employing N-nitrosobutylurea which is known for its carciogenetic action. Female Wistar rats were divided into 4 groups, namely, group with total thyroidectomy, group receiving thyroid sic (without thyroidectomy), group receiving thyroxin (without thyroidectomy), and control group. The thyroidectomy was done 14 weeks after birth and, thyroid sic and thyroxin started to be administered 14 weeks and 8 weeks after birth, respectively. Thyroid sic and thyroxin were given 0.5mg/day/rat and 10μg/100g body weight for 5 days in a week, respectively. N-nitrosobutylurea of 6mg/day/rat was administered to the rats of all the groups for 5 days in a week from the 6th post-natal week. In patients with various breast diseases functional levels of the thyroid gland were determined by measuring triiodothyroxine (T(3)), Thyroxin-binding-globulin (TBG), thyroid-stimulating-hormone (TSH) and prolactin. Blood samples were obtained in early morning and, serum samples obtained were frozen and stored. T(3), TSH and prolactin were measured by radioimmunoassay, triosorb test by resin-sponge uptake method and TBG by electrophoresis with cellulose acetate membrane. The patients studied consisted of 8 with fibroadenoma mammae, 4 with mastopathy and 52 with breast cancer. Results obtained in the study are as follows: 1) Carciogenetic action of N-nitrosobutylurea in rats with total thyroidectomy was greater at the beginning of the administration, but long-term administration of N-nitrosobutyl reduced its carciogenetic effect. 2) There was no carciogenetic influence in NBU on rats with thyroid sic administration. 3) No carciogenetic effect was found in rats with subcutaneous administration of thyroxin. 4) TSH and PRL were higher in patients with breast cancer, particularly in earlier stage, compared with those in patients with benign breast diseases, including mastopathy and fibroadenoma mammae. However, there was no correlation of TSH and PRL levels to stages of the breast cancer. 5) PRL was elevated after radical mastectomy than that before the operation. 6) There was no significant difference in T(3), triosorb test and TBG between patients with the benign diseases and those with breast cancer. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 クモ膜下出血に伴う交通性水頭症の発生機序に関する実験的研究 1165 1178 EN Kenji Suzuki Subarachnoid hemorrhage following rupture of aneurysm is one of the significant factors that cause communicating hydrocephalus. The present study describes the mechanisms of communicating hydrocephalus following subarachnoid hemorrhage. Using 26 adult mongrel dogs, cisternal puncture was percutaneously made through which about 10ml of autologous whole blood was injected aseptically. Three to five weeks after the injection, animals were served for the experiments as a chronic subarachnoid hemorrhage group. Subacute subarachnoid hemorrhage group was obtained one week after the injection. Normal animals without cisternal puncture (or subarachnoid hemorrhage) were served as control. Laminectomy at the second cervical level was performed, and two silicone tubes were inserted upward into the cisterna magna (intracranial subarachnoid space) and downward into the spinal subarachnoid space. Then, extradural ligation of the spinal cord was made with silk threads at two different places to completely separate the subarachnoid space into the intracranial and the intrathecal portions. Saline solution was injected through each tube and at the same time changes in the CSF pressure were continuously recorded with transducers. Control cases: In the intracranial space, the elevated pressure (about 2000mmH(2)O) after injection of saline solution rapidly dropped to the level of 100-150mmH(2)O for the period of 2-3 min. In the intrathecal the level of CSF pressure fell down abruptly after the injection, and then decreased more slowly than in the intracranial. Acute subarachnoid hemorrhage cases: The saline solution and blood in an equal volume was injected alternately. The high CSF pressure after the injection of saline solution was sustained for the longer period, as the more volume of blood was injected. At the same number of times of blood injection, the absorption capacity of saline in the intracranial space was more easily impaired than in the intrathecal. Subacute subarachnoid hemorrhage cases: Mildly prolonged elevation of CSF pressure was recorded after the injection of saline solution in both subarachnoid spaces. However the amplitude of pulsation in intracranial subarachnoid space and the grade of initial pressure drop in intrathecal space were almost same as that of control cases. Chronic subarachnoid hemorrhage cases: Prolonged elevation of CSF pressure was seen after the injection of saline in each part. Moreover, in the intracranial space, the amplitude of pulsation of CSF pressure was higher than that in control cases. In the intrathecal, as compared with control cases, the height of initial drop in CSF pressure was more markedly increased. This phenomenon seems to have occurred due to reduced elasticity of meninges against the increased pressure by saline injection. And a distinct increase of amplitude of the pulse wave in the intracranial space of chronic cases seems to be one of the factors that would cause the ventricular dilatation along with the decrease in the elasticity of the meninges. Actually, ventricular dilatation was recognized in almost all chronic animals. It may conclude that, following subarachnoid hemorrhage, the impairment of CSF absorption in not only intracranial but also intrathecal subarachnoid spaces, the decrease of the spinal dural sac elasticity and the increased amplitude of CSF pulsation seem to play an important role in producing communicating hydrocephalus. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 RI Angiocardiographyによる心疾患の診断 第3編 左-右シャント症例について 1157 1163 EN Masatada Tanabe Toyosato Tamai Etsuo Kawase Hisao Takaki Kiichiro Mizukawa Setsuo Morimoto Keiji Hashimoto Katashi Sato Yoshio Hiraki Count ratio (C(2)/C(1)) technique for detection of left to right shunts by radionuclide was performed. Count ratio technique of ASD cases accurately detected shunts and could reliably separate from normals, but there were a few false positive in patients with ventricle septal defect. There was no correlation between C(2)/C(1) ratio and oximetry. Postoperatively, C(2)/C(1) ratios of ASD cases were resumed to normal or close to normal but some cases of VSD were not returned to normal. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 Levamisole投与による癌患者術後免疫能低下の防止 1151 1155 EN Hiroaki Miwa Tomonori Kawai Kunzo Orita Fluctuation of the postoperative lymphocyte blastformation rates against phytohemagglutinin (abbreviated as the blastoformation rate) were examined in gastrointestinal cancer patients administered with levamisole 150 mg/day, three consecutive days, every other week from the third day before operation comparing with the values of the control cases. In the cases of curative resection the postoperative blastformation rate of the control group decreased due to operation damage from the first to the third postoperative week and recovered at the fourth week. But, the postoperative blastformation rates of the levamisole group did not decrease, moreover, increased continuously to the fourth week. This result would induce the development of survival ratio in the gastrointestinal cancer patients with administered levamisole. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 RI angio-cordiographyによる心疾患の診断 第2編 僧帽弁疾患症例を中心として 1145 1149 EN Masatada Tanabe Toyosato Tamai Etsuo Kawase Hisao Takaki Kiichiro Mizukawa Setsuo Morimoto Keiji Hashimoto Katashi Sato Kaname Aono 心疾患を有する患者では,その循環動態は種々の因子により容易に変化するものであり,一回の検査のみで決して正確にその患者の動態を表わしているとはいいがたい.この点コンピューターを用いたRI angio-cardiography(以下RCGと略す)は被曝線量も少く,生理的状態を損うことなく繰返し検査を行うことが出来る非観血的方法であり,心疾患者の術前術後の変化,治療経過観察に極めて有用である.従って,僧帽弁疾患におけるRCGによる右室,左室peak to peak time C(2)/C(1)比の解折は,上述の点後於て重要な臨床情報を提供すると考えられる.今回は僧帽弁疾患を中心としてこれらの点について若干の検討を試みた. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 犬のベクトル心電図の誘導法について 胴体模型による基礎的検討 1139 1144 EN Shoichi Haraoka Takashi Fujita Daiji Saito Hidenori Yoshida Yasuhiro Ogino Shunichi Kusuhara The lead vectors determined with Frank's system and McFee-Parungao system for dogs using canine torso model were studied. The dog which was used for making a torso model has a body weight of 16.2kg. The dimensions of torso model were left to right of 14.0 cm, front to back 19.5 cm and height of 44.0 cm. The center of ventricular depolarization in the canine heart was determined by the chest X-Ray film. Its position was 0.75 cm to the left, 2.9 cm to the front from the body center in the fifth intercostal space. The artificial heart dipole was placed on the ventricular depolarization center of the torso model filled with 0.1% NaCl solution. Electrode position on the torso surface were designated by two quantities: the level (number I through X) and the angle in the transverse plane are each equal to 100. The level are separated by 3 cm. The dipole was placed at Ⅵ level. Image Surface was oval and located on left anteriorly and slightly inferiorly from the heart center. Normality and orthogonality of lead vectors were examined on the torso with Frank's system and McFee-Parungao system for dogs resulting the latter to be better than the former. The effect of electrode position of transverse level was tested. An anterioly shift of these electrods resulted in a increase in X and Z components of Lead X, whereas a posterioly shift tended to decrease this dimensions. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 末端肥大症の病態並びに治療に関する研究 第二編 内科的治療に関する研究 1129 1138 EN Manabu Hanafusa Four patients with active acromegaly were treated for 18 to 180 days with 2-Br-α-ergocryptine(CB-154), and the following results were obtained. 1). Two patients showed an improvement in glucose tolerance with short term treatment with CB-154, and other patients showed a change in their voice and a reduction in soft tissue thicking with long term treatment. 2). The effect of CB-154 on LH-RH induced hGH release was studied in 2 patients with active acromegaly. In a patient whose hGH levels were suppressed by administration of CB-154 (CB-154 responder), CB-154 completely blocked LH-RH induced hGH release, whereas in a CB-154 non-responder CB-154 did not block LH-RH induced hGH release. 3). Under CB-154 administration plasma hGH concentration fell remarkably during awake, but plasma hGH level during sleep was not suppressed in patients with active acromegaly. These results suggest that CB-154 is a potent drug for the treatment of acromegaly and act on both superior centers, the hypothalamus and the pituitary gland. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 末端肥大症の病態並びに治療に関する研究 第一編 各種薬物負荷に対する血漿成長ホルモンの反応性に関する研究 1117 1128 EN Manabu Hanafusa The effects of several drugs on the release of human growth hormone (hGH) were investigated, and the following results were obtained. 1). Single intravenous injection of insulin or drop infusion of L-arginine provocated an increase in plasma hGH in 3 out of 8 and 4 out of 6 patients with active acromegaly, respectively. 2). Single intravenous injection of thyrotropin-releasing hormone (TRH) or luteinizing hormone-releasing hormone (LH-RH) provoked a prompt increase in plasma hGH in 7 out of 13 and 4 out of 10 patients with acromegaly, respectively, whereas no significant change in plasma hGH was observed in normal subjects. 3). Plasma hGH levels decreased significantly within 30 min after an injection of glucagon in 7 out of 10 patients with acromegaly. 4). Single oral administration of L-dopa or 2-Br-α-ergocryptine (CB-154) induced a significant fall in hGH levels in patients with acromegaly, but a significant rise in plasma hGH in normal subjects. 5). The hGH response to L-dopa was compared before and after incomplete removal of pituitary adenoma in 2 patients with active acromegaly. Before surgery L-dopa decreased the plasma hGH levels, while after surgery L-dopa caused a significant increase in plasma hGH levels in both patients. At 10 months after surgery L-dopa decreased the plasma hGH levels again in one patient despite a low basal hGH concentration. These results suggest that the primary disorder of the acromegaly resides in the pituitary level at least in some patients with acromegaly. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 胆汁中非bilirubin diazo反応陽性分画に及ぼすbucolomeの影響 1111 1116 EN Tadasuke Kondo Jiro Yonei Yasusuke Ishikawa Influence of bucolome on non-bilirubin fraction showing positive diazo-reaction (non-bilirubin fraction, dipyrril substances) was studied using heterozygous Gunn rats. Two hundred mg of bucolome per kg of rat body weight was injected intramuscularly once a day for four days. The bile collection by the bile duct canulation was started 24 hours after the final drug loading and continued for 22 hours. The following results were obtained: 1) Amount of non-bilirubin in the rat bile increased significantly in bucolome loading group than in the control group. 2) Radioactivity in non-bilirubin fraction in the bile after bucolome and (3)H-bilirubin loading was not increased significantly than in the control. 3) In bucolome loading group, bile volume increased significantly but the excreted amount of bilirubin did not increased. According to these results, bucolome did not catabolize bilirubin directly but increased non-bilirubin fraction, dipyrril substances, in the bile. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 抗てんかん剤（アレビアチン）による平衡障害の2症例 1105 1109 EN Shiro Shibata Tsuneo Takeda Masayuki Kajihara Ryusuke Saito Yoichi Endo Yoshio Ogura Aleviatin (Diphenylhydantoin) is well known as an excellent antiepileptic drug, especially against grand-mal convulsion. Side reaction of this drug, however, was various and the reports concerning side reaction were many. The authors have reported two patients of Aleviatin intoxication with the complaint of postural disturbance. Some discussions and comments were made on the cases and literatures. Summarizing the symptoms of these side reactions, 1. acute intoxication a. skin manifestations: rash like scarlet fever or urticaria, exfoliative dermatitis, systemic lupus erythematoid eruption, Stevens-Johnson syndrome b. neurological signs: postural disturbance, ataxia, tremor, dysarthria, diplopia, nystagmus, tinnitus, hearing impairment, choreoathetoid movement c. psychological signs: nervousness, excitement, disorientation, insomnia, pseudodementia d. blood manifestation: leucopenia, anemia, leukemoid change e. digestive disorders: thirst, nausea, vomiting, constipation 2. chronic intoxication a. gum hypertrophy b. hypertrichosis c. hyperglycemia No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 実験的高アルギニン血症ウサギに関する研究 第1編 アルギニン大量負荷ウサギの血清,尿および脳組織中のアミノ酸並びにグアニジノ化合物について 1093 1104 EN Sadayoshi Suwaki A hyperargininemia was induced by oraly administration of arginine in rabbit, and amino acids and guanidino compounds were analysed in the serum, urine and brain. As the experimental results, the following changes in the content of amino acids and guanidino compounds were observed: 1) Urea and ornithine increased, and otherwise, threonine, serine, isoleucine and lysine decreased significantly in the serum of the experimental hyperargininemic rabbit. 2) Among the serum guanidino compounds, only guanidinosuccinic acid increased significantly in the hyperargininemics. 3) Urinaly urea, alanine, cystathionine, ornithine and lysine increased significantly in the hyperargininemics. 4) Taurocyamine, guanidinosuccinic acid, glycocyamine, N-acetylarginie and γ-guanidinobutyric acid increased significantly in the urine of the hyperargininemics, and otherwise several unknown guanidino compounds were observed as higher peaks in the analysing record of the hyperargininemics. 5) Urea and ornithine increased and lysine and l-methylhistidine decreased significantly in the brain of the hyperargininemics. 6) Glycocyamine and N-acethylarginine increased significantly in the brain of the hyperargininemics, but no significant change was observed in other guanidino compounds in the brain of our experimental animal. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 90 9-10 1978 マウス白血病細胞における細胞表面構造と出芽ウイルスの電子顕微鏡的研究 ―特にフリーズエッチング法を主とした観察― 1073 1091 EN Kouji Fujio A study was made on alterations in the surface structures of Gross virus-induced murine thymic leukemia cells and budding virions with freeze-etching technique. The budding virions were clearly observed on the fractured faces of cell surface as well as microvilli and ruffled membranes. Knobs, approximately 10 nm in diameter, were demonstrated on the surfaces of budding virions. Extracellular virions had also the similar structure on their surfaces. On the fractured protoplasmic face (P.F.), 8.5-10 nm typical intramembranous particles (I.P.) were arranged randomly without detectable aggregated particles. There also appeared smooth semispherical protrusions which could be regarded as budding virions. Comparison was carried out between numbers of I.P. in leukemia cells and those in normal thymocytes, showing that I.P. slightly increased in leukemia cells. No potential conflict of interest relevant to this article was reported.