start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=5-6 article-no= start-page=127 end-page=141 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19720630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Observations on Diapedesis with A High-speed Cinema kn-title=高速度カメラによる漏出性出血の観察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Microscopic observations on diapedesis of the mesenterium in a rabbit was carried on by high speed cinephotography, where recording rate to 2200 f. p. s. were used. The majar features of diapedesis elucidated in this study were as follows: An erythrocyte which come near to a small opening on the microvascular wall transformed and was attracted by the opening. Then it extravasated from the vascular wall. The length of time required for the erythrocyte to pass through the wall ranged from 0.047 sec. to 0.31 sec.. The erythrocyte escaped with a high speed (5-7mm/sec.) as a dash at the external border of the vascular wall (comparing 110μ/sec. of erythrocyte flow). Platelet flew toward the opening without showing down its flow velocity. Diapedesis meant in this study that erythrocytes extravasated one by one from the small openings proper to diapedesis. To author's knowledge, there is no study concerning with diapedesis observed with a high speed cinema. This study was read at the 8th Japanese Association of Angiology (1968) and the 15th Japanese Symposium of Medicine (1970). en-copyright= kn-copyright= en-aut-name=ShimamuraYukio en-aut-sei=Shimamura en-aut-mei=Yukio kn-aut-name=島村幸夫 kn-aut-sei=島村 kn-aut-mei=幸夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二外科教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=5-6 article-no= start-page=119 end-page=125 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19720630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=DNA synthesis inhibiting factors of muscle-cornin and cornea-cornin kn-title=筋肉corninおよび角膜corninのDNA合成抑制因子について en-subtitle= kn-subtitle= en-abstract= kn-abstract=An attempt was made to purify the active principle (s) of cornea-and muscle-cornin, inhibiting the DNA synthesis in cultured mammalian cells. Each of the two kinds of cornin was separated into the dialysable (D) and the undialysable (U) fraction, which were further fractionated by the use of Sephadex gel. The inhibitory rate of these fractions was indicated by percent of control estimating the incorporation of (3)H-thymidine into cells or DNA fractions of cultured rat liver cells, line RLN-10, during the incubation period of 6 hours at 37℃ in the presence of respective fractions at the final concentration of 0.5 percent in the culture medium. As a result, the highest activity was shown in the D-U fraction of muscle-cornin, which corresponded to the elution peak of the inorganic phosphate, and in the D fraction of cornea-cornin, The activity, however, was also shown in several other fractions. These data suggest that the inhibitory effect of cornin on the DNA synthesis in mammalian cells is due to combined effects of several factors present in cornin. en-copyright= kn-copyright= en-aut-name=NisidaIsamu en-aut-sei=Nisida en-aut-mei=Isamu kn-aut-name=西田勇 kn-aut-sei=西田 kn-aut-mei=勇 aut-affil-num=1 ORCID= en-aut-name=MurakamiTetsuhide en-aut-sei=Murakami en-aut-mei=Tetsuhide kn-aut-name=村上哲英 kn-aut-sei=村上 kn-aut-mei=哲英 aut-affil-num=2 ORCID= en-aut-name=MakiyamaMasao en-aut-sei=Makiyama en-aut-mei=Masao kn-aut-name=牧山政雄 kn-aut-sei=牧山 kn-aut-mei=政雄 aut-affil-num=3 ORCID= en-aut-name=OhnoNaofumi en-aut-sei=Ohno en-aut-mei=Naofumi kn-aut-name=大野尚文 kn-aut-sei=大野 kn-aut-mei=尚文 aut-affil-num=4 ORCID= en-aut-name=NakaraiAkihide en-aut-sei=Nakarai en-aut-mei=Akihide kn-aut-name=半井昭英 kn-aut-sei=半井 kn-aut-mei=昭英 aut-affil-num=5 ORCID= en-aut-name=KakiuchiIchiro en-aut-sei=Kakiuchi en-aut-mei=Ichiro kn-aut-name=坪内一郎 kn-aut-sei=坪内 kn-aut-mei=一郎 aut-affil-num=6 ORCID= en-aut-name=IkemuneHironori en-aut-sei=Ikemune en-aut-mei=Hironori kn-aut-name=池宗宏典 kn-aut-sei=池宗 kn-aut-mei=宏典 aut-affil-num=7 ORCID= en-aut-name=OhtsukiHisashi en-aut-sei=Ohtsuki en-aut-mei=Hisashi kn-aut-name=大月恒 kn-aut-sei=大月 kn-aut-mei=恒 aut-affil-num=8 ORCID= en-aut-name=ChikataYoshiko en-aut-sei=Chikata en-aut-mei=Yoshiko kn-aut-name=智片芳子 kn-aut-sei=智片 kn-aut-mei=芳子 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=7 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=8 en-affil= kn-affil=岡山大学医学部第一生理学教室 affil-num=9 en-affil= kn-affil=岡山大学医学部第一生理学教室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=5-6 article-no= start-page=109 end-page=117 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19720630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Metabolism of Estrogen and Androgen in the Foetus-Placenta System and the Mother-Placenta System kn-title=胎児―胎盤系および母体―胎盤系におけるEstrogenおよびAndrogen代謝に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=There are numerous reports concerning the metabolism of estrogen in the foetus-placenta system such as the report by Diczfalusy et al. among others, but there still remain many problems unsolved as to the role of steroids that have eluded the conversion of DHA to estrogen and as to how the estrogen metabolism is conducted in the mother-placenta system. For the purpose to elucidate these problems the author studied the foetus-placenta system and the motherplacenta system each as a separate entity using labeled DHA. The results of the study are briefly summarized as follows. 1) It has been demonstrated that the conversion of androgen and estrogen from DHA into 11 steroids is being carried out in the mother's side as well as in the foetus side. 2) The activity of 16α-hydroxylase is higher in the foetus side than in the mother's side. 3) Estrogen derived from the mother-placenta system amounts to about 30% in the middle stage of pregnancy. 4) The rate of synthesis from DHA to estrogen is faster in the foetus-placenta system. 5) There can be observed no difference in the rate of androgen synthesis from DHA between the two systems. 6) The conversion of DHA to estriol is higher in the foetus-placenta system (about 2.5 times that in the mother-placenta system). 7) The conversion percentage of 16-epi-estriol, estradiol and estrone is higher in the mother-placenta system. 8) There is no significant difference in the fraction percentage of androgen between the two systems. en-copyright= kn-copyright= en-aut-name=InoharaAtuo en-aut-sei=Inohara en-aut-mei=Atuo kn-aut-name=猪原淳夫 kn-aut-sei=猪原 kn-aut-mei=淳夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部産科婦人科学室 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=5-6 article-no= start-page=95 end-page=108 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19720630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Determination of Urinary 17-Ketosteroid Fractions by Gas-Liquid Chromatography with Solid Material Insertion kn-title=固型試料投入法を用いたガスクロマトグラフイーによる尿中17-KS分画の測定に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Since the introduction of gas-liquid chromatography by Janes and Martin for the qualitaive assays of various chemical substances, this method proved to be an excellent one for isolation and identification of a variety of oily liquids as well as many other substances. The gas-liquid chromatography has been used also in the analysis of steroids affording many satisfactory results. However, this method has one great disadvantage in the when a minute quantity of urinary 17-KS (ketosteroids) is placed in liquid form it is not possible to avoid the loss of test material other than in the first trial, and also the peak of the solvent often interferes with the peak of the steroid making it difficult to make an accurate chart of the mixture of steroids of varying concentrations. Therefore, the authors devised a new gas-liquid chromatography in which the steroids are first adsorbed into a stainless steel gauze and then inserted into the specimen chamber, and we find that this eliminates the appearance of solvent peaks, as observable in the routine gas-liquid chromatography. By this method we separated and analyzed seven fractions of urinary 17-KS; namely, androsterone, etiocholanolone, DHA, 11-OH-adrosterone, 11-OH-etiocholanolone, 11-oxo-androsterone and 11-oxo-etiocholanolone. The apparatus used was the super-sensitive gas chromatograph with GC-1B solid insertion attachment and hydrogen flame ionization detector of Shimazu Mfg. Co. For the column we used 2% NGS (Shimazu product). The test material was a part of the 24-hour pooled urines. The urine was hydrolyzed by solvolysis and β-glucuronidase (the twostep hydrolysis), and the residue (neutral steroids) was sebarated into 17-KS and corticosteroids by Florisil column chromatography. Further, the 17-Ketosteroid fraction was separated into 11-deoxy-17-KS, 11-oxy-17-KS and Pd fraction by the alumina column chromatography. This chromatography serves as the prechromatography of the 2% NGS' gas-lipuid chromatography by which we can isolate DHA from 11-OH-androsterone as well as androsterone from Pd. With the fractions isolated in this manner we esperified them in the presence of trimethylsilyl ester, and we studied the ratios of anderosterone, etiocholanolone, DHA, as well as the ratio of 11-deoxy-17-KS to 11-oxy-17-KS in the 7 fractions isolated from the urines of female with normal menstrual cycle, normal adult males, and females with endocrine disorders. The results of our study may briefly be summarized as follows. In the urine of females of normal menstrual cycle adrosterone, etiochlanolone and DHA of 17-Ketosteroids tend to be higher before the ovulation and during the corpus luteum period. In the urine of normal adult male the pattern of 17-KS tended to show a high androsterone level and high androsterone ratio. In the case of Hirstism a marked increase of 11-deoxy-17-KS was observed, and the aderosterone ratio tended to show a similar value as in the male. In the cases of testicular feminization syndrome the characteristic feature was the fall in the aderosterone ratio. In the ACTH loading test. 11-deoxy-17-KS responded in the order of DHA>etiocholanolone>androsterone. Also 11-oxy-17-KS responded well. In the dexamethasone inhibition test of 11-deoxy-17-KS; DHA was inhibited most markedly, while 11-deoxy-17-KS were all inhibited uniformly. en-copyright= kn-copyright= en-aut-name=SatoShigeru en-aut-sei=Sato en-aut-mei=Shigeru kn-aut-name=佐藤繁 kn-aut-sei=佐藤 kn-aut-mei=繁 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部産科婦人科 END start-ver=1.4 cd-journal=joma no-vol=84 cd-vols= no-issue=5-6 article-no= start-page=73 end-page=83 dt-received= dt-revised= dt-accepted= dt-pub-year=1972 dt-pub=19720630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental Studies on Lactic Dehydrogenase Isozyme Part 1.The Changes of Lactic Dehydrogenase Isozyme of Gastric Mucosa during Aging Process and by Some Treatment kn-title=乳酸脱水素酵素アイソザイム（LDH isozyme）に関する実験的研究 第1編 加令及び種々の処置を加えたラット胃粘膜についての検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The incidence of gastric cancer differs greatly by the site of the stomach, and cancer has predilection for the area covering the lesser curvature. Some gastric diseases (e.g., cancer and atrophic gastritis) increase in the old age. Therefore, I have coducted a series of studies of LDH isozyme of astric mucosa in Wistar rats. The pattern of LDH isozyme of gastric mucosa separated by means of agar gel electrophoresis in the adult rat differs among topographically divided sections of mucosa. The heterogeneity of LDH is explained by the synthesis of two types of monomers, namely, H and M types. I found more of M type LDH in the lesser curvature, while more of H type LDH in the greater curvature. The changes of the isozyme pattern were observed through the aging process. It was found that the most important farction of pyloric mucosa on the lesser curvature was the slowest fraction, LD-1, and the pattern did not change by aging. Nor was there any difference in the pattern of the lesser curvature on the cardia side. On the other hand, in the mucosa of the greater curvature of newborn rats LD-1 occupied 73%, resembling the pattern of the lesser curvature, but LD-1 tended to decrease and the fast moving isozyme increased gradually through the aging process. These results indicate that M type LDH is found abundantly in the mucosa of the lesser curvature, while in the mucosa of the greater curvature the predominant pattern is converted gradually from M type LDH to H type LDH. The effect of various agents on LDH isozyme of gastric mucosa was also studied. The changes of isozyme pattern were also observed by the continuous administration of atropine and cortison. The isozyme of neighboring mucosa of experimental ulcer showed an increase of LD-1 fraction. From these findings, it is assumed that the metabolic patterns in the lesser curvature are adapted to anaerobic conditions, and these patterns resemble those of cancerous tissue. In addition, the greater curvature gradually becomes adaptable to aerobic condition during the aging process. Therefore, the differences in LDH isozyme pattern observed between the mucosa of the lesser and greater curvatures would reflect not only the morphological difference but also the difference in the specificity of metabolism accounts for more frequent development of gastric cancer on the lesser curvature. en-copyright= kn-copyright= en-aut-name=UtsunomiyaToshihiro en-aut-sei=Utsunomiya en-aut-mei=Toshihiro kn-aut-name=宇都宮俊裕 kn-aut-sei=宇都宮 kn-aut-mei=俊裕 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END