岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 筋肉から抽出した "CORNIN" の細胞分裂抑制作用に関する研究 631 644 EN Hirosi Kanao The author succeeded in the extraction of a substance from the skeletal muscle of rabbit designated as "muscle cornin", that markedly inhibits of sea urchin eggs. This substance is dialysable and can be separated into three fractions by DEAE-cellulose column. It has been found that fraction �T has no inhibitory action on mitosis, while fraction �U and �V has marked antimitotic effect, its minimum effective dose are 10(-8)g/ml. The fraction �U is a nucleoprotein with hypoxanthine or xanthine as the ba se and has the maximum absorbance of ultraviolet spectrum at 249mμ. The fraction �V is a polypeptide that has no specific maximum absorbancy of ultraviolet spectrum. The muscle cornin not only shows a typical protein wave in polarography but it also proves to be positive to ninhydrin and Liebermann reactions, bromphenol blue stain, and precipitation by trichloroacetic acid. However, it is negative to Biuret, xanthoprotein, Sakaguchi and Molish reactions. As for its sugar reaction, it is also positive to Seliwanoff and phloroglucinol reactions, but is negative to other reactions. Therefore, it has chemical properties that are partially different from those of "corneal cornin". The muscle cornin has no effect whatever on catalase activity and oxygen consumption of tissues. In addition, differing from the corneal cornin, it dose not effect the P/O ratio of rat liver mitochondria. However, just as with the corneal cornin, it dose inhibit the incorporation of (32)p into nucleic acids, especially into DNA and r-RNA of regenerating rat liver, and also it inhibits DNA synthesis. But it shows no effect on RNA synthesis. The discussion was also made on the difference in the properties of muscle cornin from those of other inhibitorsof mitosis, extracted from living tissues. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 悪性腫瘍に対する制癌剤の局所灌流療法に関する実験的並びに臨床的研究 597 630 EN Akinori Kawasaki Cancer chemotherapy by regional perfusion utilizing an extracorporeal circuit was studied experimentally and clinically, designing to investigate the local tissue tolerance, the leakage factor, the tumoricidal effect and so on. The following results were obtained. 1. The maximum safe doses of Nitrogen mustard N-oxide, Triethylene thiophosphoramide, Carzinophilin, Mitomycin C, Actinomycin D and Toyomycin when administered by perfusion into the femoral artery of dog were 5mg/kg., 2mg/kg., 2.500 units/kg., 0.8mg/kg., 80γ/kg. and 100γ/kg. respectively. 2. Under perfusion of the hind limb for periods up to 30 minutes oxygenation of the perfusing blood has nothing to do with local tissue damage. 3. On isolation perfusion under hyperthermia (40℃) or hypothermia (5℃) utilizing a heat exchanger the normal tissue damage was higher than that of normothermia. 4. The fluctuation of concentration of the drugs and the leakage factor were determined in the perfused area and systemically by means of RISA, P(32)-labeled TSPA and bio-assay of Mitomycin C. The results obtained by RISA were always higher than those of the other two, and did not indicate accurate picture in estimation of the leakage into systemic circulation. 5. The titer of Mitomycin C was unchanged by pure oxygen insufflation for 30 to 60 minutes or by the preservation mixed with whole blood in refrigerator for ten days. 6. On perfusion of the hind limb of rabbit utilizing p(32)-labeled TSPA a reversible fixation or accumulation of the drug in the perfused area was actually proved. 7. The tumoricidal effect on Brown-pearce tumor of rabbit was much evident in one-shot administration of the drug, in non-oxygenation of the blood in case of Mitomycin C, and in hyperthermic condition in case of Mitomycin C or TSPA. 8. Employing these technics twenty-nine patients with malignant neoplasms have been treated thirty-seven times. One axillary, two subclavian, fifteen pelvic, one iliac, seventeen femoral and one popliteal perfusions were included in this series. The follow-up period is too short to allow complete evaluation, but the results suggest that this method is one of the most impressive maneuver treating malignant neoplasms. 9. The undesirable effect either in the perfused area or systemically was practically negligible. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 細菌表面構造の研究 第5編 細菌表面構造の生理化学的研究 581 595 EN Kazumi Taguchi In the first item of this report, coordination of the surface substance with activity of adaptation enzyme has been studied. Purified polysaccharide-polypeptide-lipid compound had strong activity to promote the activity of adaptation enzyme, although ribonucleic acid-Mgpolysaccharide-polypeptide-lipid compound did not show any action on enzyme. Furthermore, this activity was strongest in polysaccharide-polypeptide compound. However, polypeptide or lipid had no such activity. Consequently, it was concluded that only the condition in which polysaccharide molecules were vailed on the surface of bacterial cell against both of intracellular and extracellular circumstances made it to promote the enzyme action. In the second item, the author described the bacterial toxin obtained by shaking and its relation to surface structures of the bacterial cell. By shaking bacterial cell with ribonucleic acid purified from the same kind of bacteria, there were obtained two kinds of hemolytic toxin, e. g. one contained ribonucleic acid and other contained polysaccharide. When energy substance, such as glucose, was added sufficiently, latter was predominantly produced. On the contorary, when the mixture was shaked without energy substance, former was predominantly produced. Thus, intimate relation with energy metabolism was found in the production of this kind of toxin. By shaking bacterial cell with polysaccharide-polypeptide-lipid compound, there obtained another kind of toxin which showed no intimate relation with energy metabolism. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 細菌表面構造の研究 第4編 POLAROGRAPHYに依る細菌表面構造の研究 571 580 EN Kazumi Taguchi In polargraphy, bacterial cell revealed two special phenomena; e. g. maximalization of electro-reduction potentials and adsorption of cobalt ions. The former phenomena has been less known until to date. Inteding to know the relation between this phenomena and surface structure of the bacterial cell, the author found a simple method to express accurately with inhibitory of electro-reduction potential. In hibitory activity was strkingly higher in gram negative bacterial cell than that in gram positive. and a little higher in S-type than in R-type. This tendency was completely identical with that of surface binding water which measures by dielectric spectrometory as previously reported. Same evidence was also found in the process of production of adaptation enzyme. Thus inhibitory activity of eIectro-reduction potentials was presumably due to increased binding water on the surface area of bacterial cell. On the other hand, inhibitory activity of electro-reduction potential exactly reflexed the speciality in immunological phenomena in which antigen complex was being polysaccharide-polypeptide-lipid compound. Individual kind of bacterial cell has proven to show special crossing effect in the cobalt-protein wave. As the crossing point of electro-reduction curve showed parallel tendencies with polypeptide activity on the surface of bacterial cell during process of production of adaptation enzyme, growth of the cell etc, polarography has been an effective tool to demonstrate physiolo-chemical state of bacterial surface. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 細菌表面構造の研究 第3編 細菌表面構成物質の化学的研究 551 570 EN Kazumi Taguchi Surface substances of the bacterial cell which would have biological and immunological activities were studied with aid of purification process, chemical analysis, spectrochemical procedures, viscosimetory, dielectric spectrometory, osmotic manometory and other physicochemical procedures. Purified substance from Gram positive bacterial cell by trichlor-acetic acid method was determined to be the mixture of ribonucleic acid-Mg and polysaccharde-polypeptide-lipid. Mixture was consisted of ion-binding in one-fifth and its molecular weight was ca. 400,000 with axis ratio of 1:2. By acting with ribonuclease this mixture was purified into polysaccharide-polypeptide-lipid compound which, to date, had been difficult to purify from Gram positive bacterial cell. Purified polysaccharide-polypeptide-lipid compound from Gram negative bacterial cell using trichlor-acetic acid method was also studies revealing molecular weight of ca. 150,000 and twice longer axis ratio than ribonucleic acid mixture. Thus, it was considered that parallel coexistence of two molecules of ribonucleic acid-Mg and one molecule of polysaccharide polypeptide-lipid compound was clarified. Polysacchardie-polypeptide-lipid compound was further purified into polysaccharide-polypeptide 1 (PP 1), polysaccharide-polypeptide 11 (PP 11) and lipid. From measuring molecular weight, km constant, axis ratio etc, it was found that PP 1 had more elongated molecular form than PP 11 and that relationship between PP 1 and PP 11 was identical with that between non-denaturated protein and denaturated protein. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 細菌表面構造の研究 第2編 透電スペクトルに依る細菌表面構造の物理化学的研究 519 550 EN Kazumi Taguchi In the dielectric spectra of bacterial cell, there found three kinds of abnormal distribution and absorption of electric wave which widely distributed from ultra-short wave to centimeter wave. These absorption curves were named as first, second and third curve and could be completely analyzed by Cole-Cole procedure. Thus detailed analyzed data indicated that these curves were preusmably and theoretically due to three different kinds of binding water. To determine the intracellular locations of these binding water, effect of pH on their spectra and isothermic changes of absorption curve were investigated. Fffect of pH revealed that they were completely different from each other demonstrating double dissociation curve in the first, simple dissociation curve in the second, and no special dissociation curve in the third. Isothermic changes of spectra were also analyzed by Freundlich and Cunningham's equation. It was concluded from this analysis that there were at least two kinds of absorption centre in the first, one kind in the third. However the second could not be explained by multi-molecular absorption. On the other hand, changes of spectra in agglutination reaction, difference between S-type and R-type of bacterial cell, changes during growth of bacterial cell, effects during the process of production of adaptation-enzyme were also investigatcd. Thus, it was concluded that first absorption was originated from polysaccharide in surface substance and second absorption was due to intracellular unknown substance. On the other hand, third absorption was considered to be due to polypeptide in surface substance. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 77 3 1965 腎性高血圧症に関する研究 第1編 実験的研究 451 476 EN Tohoru Kawahara Experimental studies on renovascular hypertension were made on dogs. Hypertension was successfully produced by constriting left renal artery with concomitant right nephrectomy. Left femoral-renal artery anastomosis was made with an given interval after hypertension was stabilized. Changes in blood pressure, renal clearance, serum natrium and potassium, blood nonprotein nitrogen, and urinary excretion of aldosterone were followed through the course of experiment. In addition to, effects of intravenous drip infusion of Angiotensin �U on those elements were studied on normal dogs. The following results were obtained. 1) Blood pressure was measured on right carotid artery which was displaced superficially and wrapped with skin flap in this series. Normal systolic pressure was in the range from 111 mmHg to 140 mmHg. 2) Persistent hypertension was successfully produced by constricting left renal artery with concomitant right nephrectomy and it was relieved with increase of renal blood flow by left femoral-renal artery anastomosis. 3) Renal plasma and blood flow, and glomerular filtration rate were decreased, although decrease of the last one was slight compared to that of the former two, resulting increase of filtration rate. They retruned to pre-experimental values after successful left renal artery reconstruction. 4) No marked changes were found in serum natrium, potassium, and nonprotein nitrogen. 5) Urinary excretion of aldosterone was definitely increased when hypertension was produced compared to before experiment and after revascularization of left kidney. 6) Blood pressure was elevated from ten to fifteen minutes later after beginning of drip infusion on Angiotensin �U. It was kept constantly in high level during infusion and returned to the preexperimental level after discontinuation of infusion. 7) Renal plasma and blood flow, and glomerular filtration rate decreased during Angiotensin �U infusion, although decrease of the last one was slight compared to that of the former two, resulting decrease of filtration rate. These values returned to the preexperimental level after discontinuation of infusion. 8) No marked changes were found in serum natrinm, potassium and nonprotein nitrogen was inclined to decrease during Angiotensin �U infusion, showing increase of potassium-natrium ratio which did not return to normal after discontinuation of infusion, too. 9) Urinary excretion of natrium and potassium was inclined to decrease during Angiotensin �U infusion, showing increase of potassium-natrium ratio which did not return to normal after discontinuiation of infusion, too. 10) Urinary output decreased slightly during Angiotensin �U infusion and showed some increase after discontinuation of infusion. 11) Urinary excretion of aldosterone increased definitely during 24 hours after Angiotensin �U infusion, compared to before experiment and on the 3rd day after infusion. 12) It was confirmed from the above data that mechanism of renal hypertension and stimulating factor of aldosterone excretion are due to Renin-Angictensin �U system. No potential conflict of interest relevant to this article was reported.