電気刺戟（in vitro）の脳物質代謝におよぼす影響 第3編 電気刺戟（in vitro）の大脳トランスアミネーションにおよぼす影響

1) ダイコクネズミ,タイワンザル,ヒト脳の大脳ホモジネートおよび大脳皮質切片を用いて電気刺戟(in vitro)のトランスアミネーションにおよぼす影響をみた. 2) ダイコクネズミ大脳においてはAsGT活性は電気刺戟によつてホモジネートでは14.6%切片では9.6%阻害された.すなわちホモジネートの方が切片より阻害率は著明なようであつた.またAIGT活性は電気刺戟によつてホモジネート,切片とも著変なく,γAGT活性は電気刺戟によつてホモジネート,切片とも阻害傾向を示した.そこでAIGTは酵素系が異なるためではないかと推論した.また反応率はいづれの場合も切片の方がホモジネートより減少していた. 3) タイワンザル大脳皮質ホモジネートにおいてはAsGT活性は電気刺戟によつて平均約11%阻害された.またヒト健常大脳皮質切片においてはAsGT活性は電気刺戟によつて平均約11.3%の促進をみ,ヒト萎縮大脳皮質ホモジネートにおいては殆ど変化をみなかつた.また反応率を比較するとヒト健常大脳皮質切片ではダイコクネズミの切片の場合に比し対照時,刺戟時ともに約20%高く,ヒト萎縮大脳皮質ホモジネートではダイコクネズミのそれに比しやや低い値を示した.すなわち萎縮脳のAsGT活性の低下が考えられる.

With the use of homogenate of the brains and cerebral cortex slices obtained from albino rats, Pithecus monkey and human, the author observed the influences of electrical stimulation (in vitro) on the fransamination, and obtained the following results. 1. In the case of albino rat cerebrum the electrical stimulation inhibited the synthesis of glutamic acid from the aspatic acid 14.6 per cent with homogenate and 9.6 per cent with slices. Namely, the rate of such inhibition seems to he greater in the case of homogenate than in the case of slices. In addition, in the alanine series ne marked changes could be recognired but in GABA series all tended to receive a slight inhibitory effect. In all cases, however, the rate of reactivity is diminished more with slices than with homogenates. 2. In the case of the cerebral cortex homogenate of monkey, the electrical stimulation inhibited the glutamic and production in the aspartic acid seriet about 11 per cent on the average. In the case of the normal human cerebral cortex slices, the electrical stimulation accelerated the glutamic acid production about 11.3 per cent on the average, while in the case of atrophic human cerebral cortex homogenate hardly any changes could be recognized. In comparing the rats of reactivity in the case of the normal human cerebral cortex slices it is about 20 per cent higher both in the control group and experimental group than in the case of the slices of albino rats. In the cisa of the atrophic human cerebral cortex homogenate the reactivity was slightly lower than in the case of albino rats.