岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 放射線照射時に発生する溶血性物質に就いて 第二編 溶血性物質の定性的研究並びに生物作用に就いて 1561 1567 EN Eiko Akagi The cause of irradiation anemia seems to lie in biological phenomena as well as in hemolytic toxin produced by X-Ray irradiation. It has been confirmed that since phospholipid fraction in vivo shows strong toxicity in direct proportion to the dose of X-rays, the actual hemolytic substance is lysolecithin. This substance is proved to give rise to marked morphological changes in hematopoietic organs, and it has also been clarified that the same substance acting on sea-urchin egg brings about a delay in egg division. However the same snbstance does not induce any striking change in the respiratory enzyme system. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 X線照射家兎肝より抽出せる不飽和脂肪酸分画 (OX) の筋肉内移植吉田肉腫細胞のコハク酸脱水素活性に及ぼす影響について 1555 1560 EN Yoshiko Iguchi In order to study influences of OX on the activity of succinic dehydrogenase in Yoshida sarcoma cells transplanted intramusculrally in the albino-rat thigh, the author injected OX intra-tumorally or intravenously into tumor bearing rats and carried out biochemical and histochemical investigations. The results are as follows: 1. In the cases given direct, intratumoral injection of OX, the activity of succinic dehydrogenase in Yoshida sarcoma cells thus transplanted is decreased, and it recovers very slowly but the same activity in muscle tissues recovers more rapidly. 2. In the histochemical observations when OX is injected intratumorally, central necrosis is increased and also a marked enlargement of bionecrotic foci occurs, but in the outer-most layer colonies of proliferative cells can be recognized. 3. When OX is injected intravenously into tumor bearing rats, the activity of the dephydrogenase is rather increased in tumor tissue, and the part where such increase occurred ultimately tends to become necrotic. In addition, the author discussed briefly about the relation between these changes in the activity of dehydrogenase and OX. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 鉛中毒に及ぼす2-Mercaptoethylamineの効果に関する実験的研究,Ca-EDTAの効果との比較について 第2編 ラット血液及び臓器内鉛量,ならびにモルモット尿中排泄鉛量に及ぼす影響について 1545 1553 EN Takeshi Kuroda For the purpose of elucidation the effects of MEA on the prevention and therapy of lead poisoning, the present experiment was conducted. The author first induced lead poisoning in rats and guinea pigs by administering them lead and measured quantitatively the changes occurring in the lead contents of blood, liver, kidneys, and femur in the rats and also the lead quantity excreted in urine of the guinea pigs, after administering to these animals. In addition, by administering Ca-EDTA to similar groups of animals treated in the same manner, the author compared the changes brought about by these two drugs and thus evaluated the effects of MEA. 1. At the end of the first week after repeated administration of MEA the changes in the lead contents in blood did not differ greatly from those observed in the control group, whereas those in the group repeatedly administered with Ca-EDTA showed extensively higher value. Those in the organs mentioned above did not differ greatly from each others among these three groups. 2. The lead contents in the organs 4 weeks after repeated administration of MEA did not differ from those in the control, but the contents showed the greater value of 2-3 times than the contents in the groups of repeated administration of Ca-EDTA. 3. It has been observed that the quantity of lead excreted in urine by MEA administration increases slightly as compared with that in the control group, but this increase is far lesser than that brought about by the Ca-EDTA administration. 4. From these it has been clarified that hardly any effect of dissolution and excretion of lead outside the body can be expected by the repeated administration of MEA, and that in this respect the effect of MEA is far inferior to that of Ca-EDTA. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 鉛中毒に及ぼす2-Mercaptoethylamineの効果に関する実験的研究,Ca-EDTAの効果との比較について 第1編 マウス体重,マウス生存率並びにラット体重及び血液所見に及ぼす効果について 1521 1544 EN Takeshi Kuroda The present experiment was conducted in order to study the preventive and therapeutic effects of 2-mercaptoethylamine (MEA) on lead poisoning. By inducing experimental lead poisoning in mice and rats with lead, the author investigated the effects of MEA on the body weight and survival of mice and on the body weight and the blood picture of rate similarly treated, and taking these effects of MEA as the criteria and comparing them with the effects of Ca-EDTA similarly administered, the author evaluated the effectiveness of MEA. The following are the results. 1. It has been found that the effects vary according to the species of animals as well as to the amount of lead given, and for the change in the body weight of mice it proved to be superior but for the changes in the survival of mice and in the body weight and blood picture of rats it was clearly inferior to those of Ca-EDTA. 2. Generally speaking, the effect of MEA is superior to Ca-EDTA for the first week after the initiation of its administration but when repeatedly given thereafter the effect of Ca-EDTA supercedes that of MEA. 3. Concerning the mechanism of the action of MEA, at present it is still in a theoretical stage and it remains a problem that requires more extensive studies before confirming its mechanism. However, a deduction may be drawn from the findings of the present experiment. Namely, it is assumed that the effect of MEA is due to some biological action other than its action of producing metal complex and its combining power lead in vivo is extremely weaker than that of Ca-EDTA so that it tends to bring about oxidative disintegration of lead-MEA complex in vivo and thus there is rather a possibility of its unfavorable effect. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 淋巴腺組織培養に関する研究 第1編 各種培地が健康家兎淋巴腺組織培養に及ぼす影響並に人非病的淋巴腺と各種疾患々者淋巴腺組織培養について 1479 1493 EN Teruo Kobayashi For the lymph-node tissue culture with cover-slip generally the classical media and culture technics have been used in the past. The author studied various methods of tissue culture as well as media and also applied these technics to clinic, and obtained the following results. 1. As for the cell growth (relative growth rate, the wandering velocity of lymphocytes, and the cell density) the slide with a hollow method is beat in the tissue culture by the coverslip methods, and chick embryo juice mixed with blood plasma is good in the media. As for the cell observation the simplified culture method devised in our laboratory is convenient. By Unno's method a fairly good results can be obtained for the vital observation of cells and for the cell growth. 2. In the experiments of adding various substances to the media of chick embryo juice mixed with blood plasma, utilizing the Unno's method, the author studied the effects of these substances on the cell growth. When vitamin B(12) or estradiol is added, the result in each case is better than the control (added with Ringer'a solution), and when vitamin B(1), C, Thyradin, spleen extract, or extract of lymph node are added, the results do not differ much from that of control. With the addition of ACTH the result is inferior to that of the control. 3. The result of the tissue culture of non-pathologic human lymph nodes is about the same as in the case of the tissue culture of normal rabbit lymph nodes. In the lymph-node tissue culture in a case of lymphadenitis simplex the result is far superior to that of nonpatholcgic human lymph node, and in the lymphnode tissue culture in the case of paticnts with reticulosarcoma, Hodgkin's disease, tuberculous lymph-node inflammation and cancer metastasis, the results are mostly poor. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 電気刺戟（in vitro）の脳物質代謝におよぼす影響 第4編 電気刺戟（in vifro）の大脳アミノ窒素におよぼす影響 1471 1477 EN Hisashi Kumashiro 1) ダイコクネズミ大脳皮質切片を好気的および嫌気的条件下でインクブイトし.それぞれについて基質なく.およびブドウ糖基質のもとの電気刺戟時の浮游液中のアミノ窒素量をニンヒドリン改良法により測定した. 2) 好気的で基質のない場合はインクベイト60分. 120分においてアミノ窒素は電気刺戟によつて減少傾向を示し.ブドウ糖基質の場合は.電気刺戟を加えない対照値のアミノ窒素がすでに著明な低値を示し,特異的であり.これに電気刺戟を加えると逆にアミノ窒素は増加傾向を示した. 3) 嫌気的では基質なし.およびブドウ糖基質.いづれも60分後においてアミノ窒素は電気刺戟によつて多少の減少傾向を示した. 4) 電気刺戟を加えない対照値アミノ窒素は嫌気的.および好気的基質なしの条件下では大体同様の時間的経過を示し. 30分値より60分値の方が増加していた.そしてこの時間的増加傾向は好気的.ブドウ糖基質の条件下では抑制された. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 電気刺戟（in vitro）の脳物質代謝におよぼす影響 第3編 電気刺戟（in vitro）の大脳トランスアミネーションにおよぼす影響 1463 1469 EN Hisashi Kumashiro 1) ダイコクネズミ,タイワンザル,ヒト脳の大脳ホモジネートおよび大脳皮質切片を用いて電気刺戟(in vitro)のトランスアミネーションにおよぼす影響をみた. 2) ダイコクネズミ大脳においてはAsGT活性は電気刺戟によつてホモジネートでは14.6%切片では9.6%阻害された.すなわちホモジネートの方が切片より阻害率は著明なようであつた.またAIGT活性は電気刺戟によつてホモジネート,切片とも著変なく,γAGT活性は電気刺戟によつてホモジネート,切片とも阻害傾向を示した.そこでAIGTは酵素系が異なるためではないかと推論した.また反応率はいづれの場合も切片の方がホモジネートより減少していた. 3) タイワンザル大脳皮質ホモジネートにおいてはAsGT活性は電気刺戟によつて平均約11%阻害された.またヒト健常大脳皮質切片においてはAsGT活性は電気刺戟によつて平均約11.3%の促進をみ,ヒト萎縮大脳皮質ホモジネートにおいては殆ど変化をみなかつた.また反応率を比較するとヒト健常大脳皮質切片ではダイコクネズミの切片の場合に比し対照時,刺戟時ともに約20%高く,ヒト萎縮大脳皮質ホモジネートではダイコクネズミのそれに比しやや低い値を示した.すなわち萎縮脳のAsGT活性の低下が考えられる. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 電気刺戟（in vitro）の脳物質代謝におよぼす影響 第2編 電気刺戟（in vitro）の大脳窒素代謝におよぼす影響 1455 1461 EN Hisashi Kumashiro ダイコクネズミ大脳ホモジネート及び,大脳皮質切片に, in vitroで電気刺戟を加え,アンモニヤ発生に及ぼす影響をみた.その結果,ホモジネートでは,アンモニヤ発生の増加をみ,切片では,アンモエヤ発生の減少がみられた.所が切片では同時にグルタミン生成の促進もみられた. 従つて切片の場合のアンモニヤ発生の減少と云うのは,実は生成されるアンモニヤが,グルタミンとしてとらえられる事によるのではないかと推論した.従つて電気刺戟(in vitro)は大脳皮質切片のグルタミン合成系を促進すると云うことになる. このことは更に,アンモニウムイオンとグルタミン酸ソーダを基質とした実験においても確かめられた. 又,α-Keto glntaric acidとNH4Clからのグルタミン酸生成系に通電しても,これを促進せしめるようであつた. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 電気刺戟（in vitro）の脳物質代謝におよぼす影響 第1編 電気刺戟（in vitro）の大脳組織呼吸におよぼす影響 1445 1453 EN Hisashi Kumashiro 1) K. R. P.溶液中のダイコクネズミ大脳皮質切片及びホモジネートにin vitro電気刺戟を加え,ブドウ糖及びグルタミン酸基質における組織呼吸を調べた. 2) ブドウ糖基質ホモジネートでは,電気刺戟によつて,その呼吸は70%近く促進された. 3) 大脳皮質切片の電気刺戟による呼吸促進率は, 12mMブドウ糖基質では平均463%, M50グルタミン酸基質では平均270%であつた. 4) 尚,グルタミン酸基質の場合,切片の小さい方が呼吸促進率は大のようであつた. 5) 又,基質なしでも電気刺戟によつて相当の呼吸促進がみられた. 6) ブドウ糖基質大脳皮質切片に, Augospelによる電気刺戟を加えた場合の呼吸促進率は244%であつた. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 X線照射時に発生せる細胞毒の研究について 第二編 X線照射家兎より抽出した不飽和脂肪酸分画のEhrlich腹水癌のコハク酸脱水素系に於ける形態学的変化並びに生体内反応について 1439 1444 EN Midori Shiaku In Part 1. the author investigatad the effects of the unsaturated fatty acid fraction extracted from the rabbit liver after x-ray irradiation on the succinic dehydrogenase system in Ehrlich ascites tumor cells and in the normal rabbit liver, and it was concluded that although this fraction does not greatly affect the normal liver cells, at a higher concentration it seems to induce cell degeneration which in turn results in a decrease in the respiratory enzyme activity. In the present experiment the author studied the morphological changes brought about by this substance in Ehrlich ascites tumor cell and the in vivo changes induced by it in the succinic dehydrogenase system. In conducting this experiment the results obtained by Yamamoto et al. of our department were referred to. With respect to the morphological changes in the tumor cells, it was observed that this substance acting for a short period of time will accelerate the respiratory enzyme activity transiently while when acting for a longer period, it decreases the activity. These findings agree more or less well with the results in Part 1. Even in the in vivo reactions the respiratory enzyme activity in the tumor cells is elevated temporarily and when acting for a longer period of time this substance decreases the activity. From these findings it is concluded that this substance does elevate the respiratory enzyme activity transiently but when it acts for a longer period of time, it seems to bring about a fall in the activity and further acting directly on the respiratory enzymes it induces the disorder in respiration. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 細菌のカタラーゼ活性について 1407 1422 EN Kenji Akiyama Part �T Effect of Added Substance to the Media on Catalase Activity Using Sh. flexneri 2 a and Sal. typhi 57 as test organism, the author studied the effect on calase activity of bacteria by adding a certain kind of substance into culturing media, the following results were obtained. 1) The catalass activity was not influenced by the addition of lactate, pyruvate or succinate into media, but was decreased by addtion of glucose. 2) A marked decrease in the catalase activity was observed by addition of KCN or NaN(3), in dilution of 1/3×10(-4)M; whereas no change in other enzyme activity was observed by the addition of these substance. 3) The addition of α, α'-dipyridyl or oxine, in dilutions of 20-30 mg/L, showed marked decrease in oxidative ability of bacteria of pyruvarte, and the decrease in catalase activity was simultaneously observed only in Sh. flexneri. Part �U Oxidation of glucose and alanine by catalase deficient bacteria Using Sh. flexneri 2 a, Sh. dysenteriae 4 and D. pneumoniae �U, the author studied the enzymatic properties of the catalase active and inactive bacterial cells mentioned above by means of volumetric studies of glucose and alanine oxidation and also studied the effect of these cells on normal or acatalasemic erythrocytes, especially on hemoglobin of the erythrocytes. The following results were obtained. 1) As the cells of Sh. flexneri 2 a grown on KCN or NaN(3) added nutrient agar media was lacking catalase activity, the organism could'nt decompose H(2)O(2) produced throuth the course of glucose or alanine metabolism. So formed pyruvate by the metabolism was oxidized by H(2)O(2) inversely. And hemoglobin was oxidized to methemoglobin, when erythrocytes were added to the reaction media. 2) Sh. dysenteriae 4 is failing catalase and the production of H(2)O(2) was not detected at all or only scantily on the cells grown on any kind of media. 3) D. pneumoniae �U is also failing catalase, but the production of H(2)O(2) was remarkably high. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 細菌による五炭糖の生成 1377 1391 EN Kakushi Honmatsu Using St. aureus, sh. flexneri 2a and Sal. typhi 57, the author carried out the studies on pentose formation from glucose or other substrates. The following results were obtained. 1) A fairly large amount of pentose was formed and accemulated from glucose, gluconate, glycerophosphate, lactate or pyruvate in every strain of the bacteria tested in the presence of AM or DNP in corcentrations of 3×10(-4) and 10(-3)M. 2) Pentose was supposed to be formed from glucose through gluconate in the case of St. aureus, but was probably formed through C(3)-comound in the case of Sh. flexneri 2 a and Sal. typhi 57. 3) It was postulated that AM and DNP inhibited the break-down of pentose to C(3)-compound; however, did not inhibit the synthesis of pentose from C(3)-compound. Part �U Effect of Changes in Culture Condition on Pentose Formation With the use of Sh. flexneri 2 a as test organism, the author investigated the effect of changes in culture condition on pentose formation and obtained the following results. 1) The bacterial cells cultured on glucose added media showed further oxidation of pyruvate to a less extent and was able to produce pentose to a greater extent from glucose and C(3)-compound in glucose metabolism. 2) The bacterial cells cultured on glucose and α, α-dipyridyl added media was more inferior in the further oxidation of pyruvate, and was more promoted pentose formation rate. 3) Pentose was produced to a greater extent under anaerobic condition than under aerobic condition. 4) From theee results, it could be stated that the suppresion of pyruvate oxidation accelerated the reaction rate which produced pentose from glucose through C(3)-compound. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 純粋な肺動脈弁狭窄症二例報告と文献考察 1369 1376 EN Isaack Sohn Sung-yu.ll Lim Young-Lin Lee Two typical cases of pure, isolated pnlmonic stenosis were studied by means of cardiac catheteriration during the past 12 months. On one of them the Brock type of valvulotomy was performed and this is the first case of pulmonic stenosis operated surgically in Korea. The results of the pulmonic valvulotomy was satisfactory initially. The patient had improved subjectively and venous pressure and circulation time returned to the normal level. However, 4 month postoperatively follow up study showed worsening of subjective symptoms again and re-catheterization was performed. There was an increased right ventricalar pressure. The reason of the failure of the operation was discussed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 有機燐剤中毒に関する研究 第4編 有機燐剤中毒並にOxime治療による臓器Cholinesteraseの消長 1359 1368 EN Shoichi Okazaki After studying disturbing actions of various alkylphosphates such as ethylparathion, methylparathion, EPN, TEPP, Pestox 3, malathon, diazinon and dipterex on cholinesterase of the brain, liver, spleen and muscles in mice, the author observed the reactivating effects on cholinesterase by administering PAM, PATD, PPATD, PPAPD, PAD, DAM, and MINA to these animals. The results are as follows. 1. In the organs of the animals administered with alkylphopshates in the amount about half LD(50), cholinesterase in various organs is decreased to 30-60 per eent of the normal level. It has been found that muscle cholinesterase is markedly disturbed in the ease of TEPP administration, while the disturbance of brain cholinesterase is markedly less in the administration of Pestox-3. 2. The brain cholinesterase disturbed by alkylphosphates can be brought back to a certain degree with administration of oxime cempounds. Especially in ethylparation poisening the effect is just as good as thaf of PAM, brining back almost to the normal level. 3. The recovery rate of the brain cholinesterase parallels most closely with the theapeutic effect, judging from the mortality rate. 4. The behavior of cholinesterase differs according to the combination of alltylphosphates and oixme compounds and to different organs, but with an exception of Pestex-3, cholinesterase recovers in some organ. Only in the case of DAM and MINA there can be observed no fluctutions at all exepting muscle cholinesterase. 5. The reactivating action of oximes on the vesceral cholinesterase is only transient. 6. PAD is less effective on alkylphospate poisonning than PAM, and the concurrent use of PAD with any one of quarternary oximes does not enhance the recovery of cholinesterase activity. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 有機燐剤中毒に関する研究 第3編 Oxime化合物の有機燐剤中毒に対する効果 1351 1357 EN Shoichi Okazaki By selecting 4 oxime compounds possessing pyridine nucleus in the molecular construction, namely, PATD, PPATD, PPAPD, and PAD, the author compared their therapeutic effects on alkylphosphate poisoning with the effect of PAM. 1. As for the therapeutic effect on alkylphosphate poisoning in mice, when administered in the maximum safety dose, PATD and PPATD are superior to PAM, but on scrutinous examinations there can be recognized not any significant difference. Effect of PPAPD is about the same as that of PAM. 2. Both PAM and PATD are most effective when administered concurrently with the intravenous administration of alkylphosphate, but as the time before or after the administration of alkylphosphate lengthens, the effect of drugs decreases proportionately. 3. Atropine used concurrently with these oxime compounds yields no better results than without it. 4. The effect of PATD is slightly more lasting than PAM, but the difference is not significant. 5. PATD has been proven to possess a reactivating action on rabbit blood cholinesterase just as PAM. Consequently it is assumed that the effective mechanism of both drugs is same. 6. When an equal dose of PAD and PAM is administered to the mice with ethylparathion poisoning, PAD is inferior to PAM in improving the mortality rate of the mice. 7. There seems to be no benefit in administering intramuscularly 15 mg/kg PAD concurrently with the administration of various oximes. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 有機燐剤中毒に関する研究 第2編 PAM (pyridine-2-aldoxime methiodide) の毒性並に生体内分布 1343 1349 EN Shoichi Okazaki In the use of any drug there always arise problems of its effect and side-effect as well as its toxicity and distribution in vivo. Therefore, the author studied these problems on PAM from various angles and obtained the following results. 1. The median lethal dosage (LD(50)) of PAM on mice has been found to be: 159 mg/kg in the case of intravenous administration; 247 mg/kg in peritoneal injection; 340 mg/kg in subcutaneous injection; and 1960 mg/kg in oral administration, proving that the limit is sufficiently within the range of commonly used drugs. 2. Histological changes occurring after PAM adminstration are consisted mainly of disturbances in the circulatory system, namely, hyperemia, congestion and slight hemorrhage, but rarely of parenchymal disturbances. 3. When a large amount of PAM is administered to rabbits, there occurs a rise in the blood cholinesterase activity at first later followed by its fall. However, the fall being down to -25 per cent, it can not be considered as due to the toxicity of PAM. 4. In the oral adminstration of PAM to rabbits, PAM can be detected considerably in stomach, intestines, and in their contents, but it is distributed fairly evenly in other viscera. Moreover, in the case of the intravenous administration of PAM is also distributed in similar manner. It diminishes relatively promptly from the body fluid. Judging from these findings as well as from results of clinical observations, there is no danger of toxicity of PAM in the amount as used in our clinic. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 有機燐剤中毒に関する研究 第1編 Parathion中毒に対するPAMの臨床効果 1329 1341 EN Shoichi Okazaki On 39 cases of parathion poisoning treated with PAM (pyridine-2-aldoxime methiodide) the author obtained the following results. 1. From cholinesterase determinations and clinical findings PAM has been found to be the most effective drug available at present for the treatment of parathion poisoning. 2. The effect appears promptly within 30--40 minutes of the treatment. 3. By administering PAM such symptoms as disturbance of consciousness, muscular fasciculation, salivation, bronchial secretion, convulsions, vomiting, and rasping in the lung, all disappear easily. Apart from these there may persist for quite same time such subjective symptoms as headache, miosis, pallor, quick breathing, speech difficulty, slow pupillary reflex, and numbness of limbs, but these are not dangerous. 4. If no improvement at all occurs after the administration of PAM, the case may be suspected as not paration poisoning. 5. As for the method of administration, 1.0 g PAM (40 ml 2.5% aqueous solution) is administered intravenously, and if no prompt effect appears another dose of 1.0 g PAM will be sufficient. 6. Occasionally vomiting accompanies the PAM injection, but it is considered as due to parathion, and there can be observed otherwise no other side-effect. 7. There is no need of concurrent use of atropine, and even in severe case the dose of atropine should be limited to 0.5-1.0 mg to suppress secretion. The use of a large amount of atropine, on the contrary, brings about the danger of atropine poisoning. 8. In extremely severe cases where even 2.0 g PAM shows little effect, PAM in the amount up to 4-5 g may be additionally given along with atropine. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 S.enteritidis超遠心分劃物質の免疫学的研究 第2編 感染防禦実験 1319 1327 EN Tatsuji Yoshioka Using the same method described in the part I, the author prepared supernatant (Sf) and particulated (Rf) fractions from MURASHIMA's strain and TAKATA's strain respectively. And studied the protective antigenicity of these fractions. The results obtained were as follows. 1) No productive antigenicity was observed on the inoculatoin of Sf. However, the inoculation of Rf could give sufficient immunity by a single intraveneous inoculation: interperitoneal inoculation was inferior than intraveneous one in its effect. 2) By repeated inoculation of Rf from the highly virulent strain the animal became sensitive to a challenge of bacteria. Meanwhile repeated inoculation of Rf from the less virulent strain could give immunity enough to overcome challenge. 3) The prcductive antigenicity of Rf was inactivated either by exposure to heat, 56℃ 30 min, or by action of formalin or mersonin. 4) Sufficient immunity to prevent oral infection was acquired by inoculation of Rf from the highly visulent strain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 S.enteritidis超遠心分画物質の免疫学的研究 第1編 感染防禦実験のための予備的検討 1313 1318 EN Tatsuji Yoshioka In order to study biochemical and serological properties of supernatant (Sf) and particulated (Rf) fractions of Sal. enteritidis, the author prepared two fractions by ultracentrifugation of ground cell suspension of the growing cell of two organisms. The organisms used throughout this study were selected among several strains of Sal. enteritidis in the light of virulence to mouse and were MURASHIMA's strain, which showed the highest virulence and TAKATA's strain, showed the lowest virulence. The following results were obtained. 1) Nitrogen and carbehydrate contents were very low in Rf compaired to Sf, especially it was markedly low in the case of nitrogen. 2) Rf showed oxidative ability only to succinate and lactata among the substrate related to TCA cycle, but Sf could oxidize many substrates tested in various degree. However, Rf Sf and of TAKATA's strain could oxidize only succinate in high degree. 3) The specific enzyme activity of Rf was not affected by irradiation of ultraviolet ray, but was decreased to 1/3~1/4 by exposure to heat, 56℃ 30 min., or by action of formalin or mersonin. 4) Antigenic capacities differed only little between Rf and Sf in the light of aggulutinin production to rabbit serum. 5) Rf gave less intense cutaneous reaction and was less virulent than Sf in examinations using rabbit, for the cutaneous test, and mouse, for the virulence test. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 放射線照射家兎肝より抽出せる細胞毒のウニの受精及び卵割に与える影響について 1307 1311 EN Satimaru Seno Michio Yamamoto Kozo Utsumi Midori Shiwaku Eiko Akagi Yoshiko Iguchi Junichi Kobayashi Hisako Ueno The effects of the OX substance, which was extracted from the liver of irradiated rabbits, on the sperm and egg, unfertilized and fertilized eggs of sea urchin (Hemicentrotus pulcherrimus) have been observed. 1. The sperms exposed to OX showed a decreased mortility and lost the ability to stimul to the egg to raise up fertilizing membrane suggesting the inability of giving physical shock to the egg surface or the damage of acrosome. 2. The unfertilized eggs exposed to OX retain the ability of the formation of fertiliging membrane and cleavage but showed remarkable delay in both the length of time for the membrane formation and cleavage. 3. The most sensitive effects on the fertilized eggs have been observed on the initial stage of fusion of sperm and egg nuclei and the metaphase. Immediately after the fertilization in the egg exposed to OX the fusion of sperm and egg nuclei was inhibited and in the cells on metaphase the cleavage was delayed by the prolonged period of transition from metaphase to telophase. 4. From the results with the consideration of the effects of X-rays on cleavage appearing in the past report the authors concluded the OX will be the essential substance representing the effects of X-rays on the cell cleavage. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 脳の遊離アミノ酸について(XIII) ヒト脳各部位の遊離アミノ酸およびその関連物質 1299 1306 EN Hirosuke Nishioka By means of ion exchange column chromatography the author carried out quantitative analyses of 14 kinds of free amino acids including the related compounds using the brain of the person who died of acute loss of blood. The parts of the brain used for the analysis were frontal cortex, corpus callosum, caudate nucleus, globus pallidus, thalamus, hypothalamus and medulla oblongata. The results are as follows. 1. In corpus callosum which contains little cellular components extremely minute quantities of phosphoethanolamine, aspartic acid, glutamic acid and γ-aminobutyric acid could be detected. 2. In globus pallidus a surprisingly large quantity of γ-aminobutyric acid could be found and it was far greater than that contained in hypothalamus. 3. In medulla oblongata only small quantities of phosphoethanolamine, aspartic acid, and glutamic acid could be detected. Likewise γ-aminobutyric acid was found not so abundant. This seems to be due to the fact that the present experiment was conducted with medulla oblongata including white matter. 4. Although only in a small quantity, cystathionine could be assayed in all these parts except globus pallidus and thalamus. 5. Even from the comparative biochemistry the present quantitative analyses gave an interesting contrast to the values obtainable in the brains of lower animals. 6. Although it was difficult to recognize any distinct difference in the pattern of amino acids between the adult brain and the infant brain, there was a clear-cut difference in the amino acid pattern of the adult brain and that of the fetal brain. Namely, in the adult human brain there exist far greater quantities of aspartic acid, glutamic acid, γ-aminobutyrie acid, and N-acetylaspartic acid than those in the fetal brain and conversely far less quantities of phosphoethanolamine and taurine than in the latter. Likewise tyrosine detected in the fetal brain could not be recognized in the adult human brain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 5-7 1960 脳の遊離アミノ酸について(Ⅻ)甲状腺剔除の脳遊離アミノ酸およびその関連物質におよぼす影響 1293 1298 EN Hirosuke Nishioka With the purpose to study the influence of thyroidectomy on the free amino acids and the related compounds the author performed the assay of 11 of these substances by ion exchange column chromatography, using the rat brains as the material. As the result it has been found that aspartic acid, glutamic acid, alanine, serine, N-acetylasparticacid, and glycerophosphoethanolamine, the substances that were decreased in the brains of hypophysectomized animals, have been maintained at the normal level on thyroidectomy. No potential conflict of interest relevant to this article was reported.