岡山医学会Acta Medica Okayama0030-15581057-81993第24回 岡山リウマチ研究会819822ENNo potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993温熱感受性の異なる培養細胞の DNA 合成に対する温熱効果―フローサイトメトリーによる研究―771778ENSeiryouTanakaThe effect of heat on DNA synthesis in HeLa S3 cells and L-5 cells was studied using flowcytometry. When D(0) values obtained from survival curves of Ehrlich ascites tumor cells, L-5 cells, HeLa S3 cells and NIH3T3 cells after heating to 43, 44, or 45℃, were compared, HeLa S3 cells were resistant and L-5 cells were sensitive to heating. During heating at 43℃, DNA synthesis (BUdR uptake) of HeLa S3 cells was resistant compared to that of L-5 cells. When the period of DNA synthesis was divided into 3 fractions (early S, mid S and late S), late S phase was the most sensitive fraction to heating at 43℃ for 60 minutes. From these results, relationship between DNA synthesis and thermocytotoxic effects are discussed.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993気管支喘息における血清抗 IgE 自己抗体の基礎的並びに臨床的研究759770ENKoichiYamagataTo clarify the pathogenesis of asthma especially intractable asthma, we measured the serum levels of anti-IgE autoantibody in 46 asthmatics and 39 healthy subjects, using solid-phase enzyme immunoassay. The serum levels of anti-IgE autoantibody in bronchial asthmatics were significantly higher than those in healthy subjects (p<0.01). The levels of anti-IgE autoantibody in 25 atopic asthmatics were also significantly higher than those in 19 non-atopic asthmatics (p<0.01). Futhermore, significant correlation was observed between the levels of anti-IgE autoantibody and serum IgE (r=0.576, p<0.01). House dust and Candida (p<0.01) specific IgE (RAST) positive asthmatics also tended to have higher serum levels of anti-IgE autoantibody. However, no significant relation was observed between the serum levels of anti-IgE autoantibody and the severity of asthma. Moreover, anti-IgE autoantibody was not significantly related to specific IgG subclass antibody or lymphocyte blastogenesis to mite and Candida, or with the numbers of eosinophils and basophils in peripheral blood. These findings suggest that anti-IgE autoantibody play an important role in the pathogenesis of bronchial asthma, especially atopic asthma associated with IgE-dependent mechanisms.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993アレルギー性胃腸症の診断に関する研究 第2編 腸音分析法を用いたアレルギー性胃腸症の診断に関する検討751758ENKatuhiroMiyashitaOral challenge with specific allergens is generally used to diagnose allergic gastroenteropathy, but no evaluation criteria have yet been established. Therefore, we examined allergological tests (skin tests, IgE RAST score), oral challenge tests with causative allergens and oral administration tests using sorbitol as a nonspecific stimulator or distilled water as a control. Phonoenterograms were recorded intermittently for up to 5 hours after the tests and analyzed by a pulse density program. The results showed that it is possible to classify allergic gastroenteropathy into six subgroups by the allergological tests, symptoms and intestinal motility analyzed by phonoenterography. Increase of bowel sounds with or without positive allergological test and symptoms indicates a high suspicion of allergic gastroenteropathy. These data suggest that phonoenterography is useful in the diagnosis of this disease. It should also be possible to diagnose latent cases of allergic gastroenteropathy by non IgE-mediated allergic reactions.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993アレルギー性胃腸症の診断に関する研究 第1編 胃腸管の過敏性における腸音分析法の有用性に関する検討741749ENKatuhiroMiyashitaAllergic gastroenteropathy has various gastrointestinal symptoms caused by allergic reactions in the gastrointestinal tract after oral ingestion of specific allergens, but no diagnostic examination has yet been established. To evaluate gastrointestinal motility as a method of diagnosing allergic gastroenteropathy, bowel sounds caused by oral ingestion of distilled water, sorbitol or allergens were recorded intermittently for up to 5 hours after oral administration tests and analyzed by pulse density program. The results showed that bowel sounds in many patients with allergic gastroenteropathy increased much more than that in normal subjects when sorbitol was ingested. The increase in bowel sounds was even more marked after oral ingestion of allergens compared to that after distilled water. These data suggest that phonoenterography after ingestion of sorbitol is useful in screening for allergic gastroenteropathy, and also using this method with ingestion of specific allergens is effective for confirming the diagnosis of this disease.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993反復する慢性中耳炎耳漏の細菌学的検討733739ENMasakazuKosakaMicroorganisms were investigated in 44 patients with chronic otitis media (COM) and repeated otorrhea. The same species were isolated most frequently from those with repeated otorrhrea (24 of 44 cases). The species were Staph. aureus (10 caces), P.aeruginosa (9 cases), Staph. epidermidis (4 cases), and P.cepacia (1 case). For each species isolated from the same patient, similar sensitivity to antibiotics were observed. Therefore, we considered that reinfection by the bacteria existing in the body caused otorrhea in some cases. After NFLX was administered for MRSA infection in COM, Cephem antibiotics (CEZ, LMOX, etc) showed a recovery of sensitivity to Staph. aureus. We observed a superinfection by Methicillin-Cephem-Resistant Staph. epidermidis (MRSE) after antibiotics therapy for MRSA in COM.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993PCR-RFLP 法による HLA-DNA-DQ タイピングと腎移植715731ENKeiichiHirakawaTwo methods of HLA-DNA typing (PCR-RFLP method and PCR-SSO method) were performed on HLA-DQ in living related, living unrelated and cadaveric renal transplants. These two DNA typing methods allowed more accurate and more detailed typing than the conventional typing method. The effect of DNA histocompatibility of DQA 1 and DQB 1, both typed by the PCR-RFLP method, on clinical outcome and surviving graft rate of renal transplant patients was analyzed. There was no correlation found between the number of mismatches between donor and recipient of DQA 1 typing in cadaveric renal transplants, of DQB 1 typing in cadaveric, living unrelated and living related transplants and the clinical outcome nor the surviving graft rate of renal transplant patients. Both the clinical outcome and the surviving graft rate in the group in which DQ 5 mismatch between donor and recipient was positive were statistically poorer than that in the group in which DQ 5 mismatch was negative. This result suggests the existance of DQ 5 mismatch between donor and recipient greatly influences graft survival after renal transplantation.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993Adenosine の内皮依存性冠血管拡張作用―麻酔開胸犬を用いた検討―705714ENNaotsuguObayashiTo evaluate the endothelium-dependent coronary vasodilatory effect of adenosine, especially the role of endothelium-derived nitric oxide (EDNO), the dose-response relationship of exogenous adenosine was examined in open-chest dogs before and after intracoronary administration of N(G)-nitro-L-arginine (NNLA), a potent inhibitor of NO synthesis. NNLA attenuated the vasodilatory effect of acetylcholine to less than 25% of the control, which indicates NO synthesis inhibition. NNLA caused a rightward shift of the adenosine dose-response curve with a significant increase of EC(50), whereas there was no significant change in the slope of the regression line calculated by log-logit transformation. These findings suggest that the coronary vasodilatory effect of adenosine is partially induced through an increase of NO release, and this action may play a role in the regulation of coronary vascular tone.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993右室心筋酸素代謝の不均一性に関する検討695703ENShinjiUchidaTo determine whether the right ventricular (RV) myocardial oxygen metabolism was heterogeneous, oxygen extraction ratios (EO(2)) and regional myocardial blood flow (RMBF) of the basal, middle and apical regions of the RV free wall were studied in open-chest dogs. After control studies, the following interventions were applied; 1) atrial pacing (200 beats/sec), 2) intravenous infusion of isoproterenol (1.0μg/㎏/min) and 3) pulmonary artery constriction to raise RV systolic pressure tp 40-50mmHg. The venous blood from three anterior cardiac veins was sampled directly, and RMBF was measured using colored microspheres. EO(2) was significantly lower in all areas of the RV compared to that in the left ventricle (LV). In control, the basal region of the RV extracted more oxygen than the apical region (p<0.01), and RMBF tended to be lower in the basal than the apical. Thus, myocardial oxygen consumption in the control did not differ in each area of the RV. During all interventions, greater increases in EO(2) were observed in the apical and middle regions than in the basal region, and RMBF increased by a similar amount in all three regions. Therefore, we suspected that oxygen metabolism in the RV was dependent on not only RMBF but also EO(2), and varied from region to region. This heterogeneity of oxygen metabolism tended to disappear under RV overload.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993肥大型心筋症における肥大様式とベクトル心電図の対比検討681693ENTeruoShirakiWe compared vectorcardiographic (VCG) findings with the distribution of hypertrophy in patients with hypertrophic cardiomyopathy (HCM). Distribution of left ventricular hypertrophy was determined by both echocardiogram (UCG) and magnetic resonance imaging (MRI). According to the specific hypertrophic site evaluated by UCG and MRI, 43 patients with HCM were classified into 4 types : septal, anterior, posterolateral or apical type. In patients classified as anterior, posterolateral and apical types, the QRS loop was directed toward the hypertrophic site because of increased electromotive force in the hypertrophic site. In contrast, in patients classified as septal types, the QRS loop was directed away from the hypertrophic site. This opposite direction was probably due to altered electromotive force and/or conduction disturbance in the hypertrophic site as the disease progressed. The present study indicates that QRS loop direction suggests the location of hypertrophy. The T loop was most slender in patients with apical hypertrophy and the slenderness was associated with the relative degree of hypertrophy in the apex compared with hypertrophy in other sites.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993ニカラベンの放射線防護剤としての有用性の検討―マウス生存率，脾コロニー，末梢血液像，脂質過酸化について―673680ENYasutaneMoriHitoshiTakashimaHiroyukiSeoMotoomiOhkawaMasatadaTanabeGokiYamamotoThe degree of radiation protection from Nicaraven after whole-body irradiation was investigated in C3H mice. Nicaraven is a free radical scavenging agent, which was shown to improve brain edema and blood flow. Nicaraven was injected intraperitoneally in mice before and/or after whole-body irradiation with 640cGy or 740cGy using Toshiba Lineac LMR-4C (4MV, 6.45×10(-2)C/㎏/min). The 30 day survival ratio was improved significantly by Nicaraven (P≦0.02). Endogenous spleen colony formation was investigated after 640cGy. The agent was injected before (pre), after (post) or before and after irradiation (pre-post), and compared with the untreated group (control). Nine days after irradiation, the mean colony formation was 2.00 (pre), 3.09 (post), 4.31 (pre-post) and 1.47 (control). The differences between pre and post (p≦0.01), between pre-post and control (p≦0.01) and between post and control (p≦0.05) were significant. Nicaraven induced recovery of leukocyte and lymphocyte counts after irradiation, but not that of erythrocytes. The effect of the agent on mice liver mitochondrial lipid peroxidation was also investigated. The lag time was not shortened, but reduction of the TBA value was observed. Nicaraven was not only recognized as a radioprotector, but its ability to promote recovery from the damage caused by irradiation was also suggested.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993サルコイドーシス肺リンパ球の Propionibacterium acnes に対する反応性に関する研究 第2編 P. acnes に対する反応性と臨床検査との相関665671ENTsuyoshiMaetaAlveolar lymphocyte response by Propionibacterium acnes (P. acnes) in patients with active sarcoidosis was previously reported by our group. The response of alveolar lymphocytes in 34 untreated patients with sarcoidosis was studied in correlation with clinical findings on radiographic, physiologic and bronchoalveolar lavage. There was a significant correlation between lymphocyte blastogenesis and the number of lymphocytes (p<0.05), and CD4(＋) T-cells (p<0.01) obtained by bronchoalveolar lavage. Furthermore, the response was significantly correlated with the activity of Interleukin-2 released by alveolar lymphocytes stimulated by P. acnes (p<0.05). In patients who showed abnormalities on all three clinical examinations, i. e. serum angiotensin converting enzyme activity, number of alveolar lymphocytes and 67Ga scintigraphy of the lung, the response was significantly higher than in the controls (p<0.001) or in patients with none of these abnormalities (p<0.01). These data suggest that the response of alveolar lymphocytes reflects the activity of pulmonary sarcoidosis.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-15581057-81993サルコイドーシス肺リンパ球の Propionibacterium acnes に対する反応性に関する研究 第1編 P. acnes に対する反応の特異性に関する検討657663ENTsuyoshiMaetaThe fact that alveolar lymphocytes in patients with active sarcoidosis are sensitized by Propionibacterium acnes (P. acnes) was previously reported by our group. Therefore, the responses of alveolar lymphocytes induced by Nocardia rubra and Streptococcus pyogenes were investigated and compared to those of P. acnes. The mean response rate of alveolar lymphocytes to P. acnes was significantly enhanced (1.89±1.45) in 12 untreated sarcoidosis patients compared with the response rates to Nocardia rubra (0.87±0.47) and Streptococcus pyogenes (0.64±0.30). Peripheral lymphocytes did not respond to any cell wall components of these organisms. Our findings indicate that alveolar lymphocytes in sarcoidosis are specifically sensitized by P. acnes, and suggest that P.acnes plays an important role in the induction of alveolar lymphocytes in sarcoidosis.No potential conflict of interest relevant to this article was reported.