start-ver=1.4
cd-journal=joma
no-vol=28
cd-vols=
no-issue=6
article-no=
start-page=673
end-page=681
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201411
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Endothelin as a local regulating factor in the bovine oviduct
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17ƒÀ increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.
en-copyright=
kn-copyright=

en-aut-name=YamamotoYuki
en-aut-sei=Yamamoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KohkaMisa
en-aut-sei=Kohka
en-aut-mei=Misa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KobayashiYoshihiko
en-aut-sei=Kobayashi
en-aut-mei=Yoshihiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=Woclawek-PotockaIzabela
en-aut-sei=Woclawek-Potocka
en-aut-mei=Izabela
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=OkudaKiyoshi
en-aut-sei=Okuda
en-aut-mei=Kiyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

affil-num=1
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=2
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=3
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=

affil-num=4
en-affil=Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
kn-affil=

affil-num=5
en-affil=Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=endothelin converting enzyme
kn-keyword=endothelin converting enzyme
en-keyword=endothelin receptor
kn-keyword=endothelin receptor
en-keyword=epithelial cell
kn-keyword=epithelial cell
en-keyword=ovarian steroids
kn-keyword=ovarian steroids
en-keyword=oviductal contraction and relaxation
kn-keyword=oviductal contraction and relaxation
END