Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes

Yamada, Hiroshi; Osaka, Hirona; Tatsumi, Nanami; Araki, Miu; Abe, Tadashi; Kaihara, Keiko; Takahashi, Ken; Takashima, Eizo; Uchihashi, Takayuki; Naruse, Keiji; Takei, Kohji

doi:10.3390/cells13161373

Permalink : http://escholarship.lib.okayama-u.ac.jp/67607

ID	67607
フルテキストURL	fulltext.pdf 4.54 MB
著者	Yamada, Hiroshi Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap Osaka, Hirona Graduate School of Science, Nagoya University Tatsumi, Nanami Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Araki, Miu Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Abe, Tadashi Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaihara, Keiko Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Takahashi, Ken Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap Takashima, Eizo Division of Malaria Research, Proteo-Science Center, Ehime University Uchihashi, Takayuki Graduate School of Science, Nagoya University Naruse, Keiji Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID publons researchmap Takei, Kohji Department of Neuroscience, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID publons researchmap
抄録	Synaptopodin 2-like protein (SYNPO2L) is localized in the sarcomere of cardiomyocytes and is involved in heart morphogenesis. However, the molecular function of SYNPO2L in the heart is not fully understood. We investigated the interaction of SYNPO2L with sarcomeric alpha-actinin and actin filaments in cultured mouse cardiomyocytes. Immunofluorescence studies showed that SYNPO2L colocalized with alpha-actinin and actin filaments at the Z-discs of the sarcomere. Recombinant SYNPO2La or SYNPO2Lb caused a bundling of the actin filaments in the absence of alpha-actinin and enhanced the alpha-actinin-dependent formation of actin bundles. In addition, high-speed atomic force microscopy revealed that SYNPO2La directly bound to alpha-actinin via its globular ends. The interaction between alpha-actinin and SYNPO2La fixed the movements of the two proteins on the actin filaments. These results strongly suggest that SYNPO2L cooperates with alpha-actinin during actin bundle formation to facilitate sarcomere formation and maintenance.
キーワード	SYNPO2L actinin actin sarcomere cardiomyocyte
発行日	2024-08-17
出版物タイトル	Cells
巻	13巻
号	16号
出版者	MDPI
開始ページ	1373
ISSN	2073-4409
資料タイプ	学術雑誌論文
言語	英語
OAI-PMH Set	岡山大学
著作権者	© 2024 by the authors.
論文のバージョン	publisher
PubMed ID	39195263
DOI	10.3390/cells13161373
Web of Science KeyUT	001305166200001
関連URL	isVersionOf https://doi.org/10.3390/cells13161373
ライセンス	https://creativecommons.org/licenses/by/4.0/
Citation	Yamada, H.; Osaka, H.; Tatsumi, N.; Araki, M.; Abe, T.; Kaihara, K.; Takahashi, K.; Takashima, E.; Uchihashi, T.; Naruse, K.; et al. Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes. Cells 2024, 13, 1373. https://doi.org/10.3390/cells13161373
助成機関名	Ministry of Education, Culture, Sports, Science and Technology Okayama University Central Research Laboratory Ehime University Proteo-Science Center (PROS)
助成番号	JP24K01309 JP23K27170 JP22K06580