Acta Medica Okayama 1344-7610 59 1 2009 Mapping of the eibi1 gene responsible for the drought hypersensitive cuticle in wild barley (Hordeum spontaneum) 21 26 EN Guoxiong Chen Takao Komatsudu Mohammad Pourkheirandish Mohammad Sameri Kazuhiro Sato Tamar Krugman Tzion Fahima Abraham B. Korol Eviatar Nevo Segregation analysis showed that eibi1, a drought hypersensitive Cuticle wild barley mutant, was monogenic and recessive, and mapped in two F, Populations, one made from a cross between the mutant and a Cultivated barley (cv. Morex), and the other between the mutant and another wild barley. A microsatellite marker screen showed that the gene was located oil barley chromosome 3H, and a set of markers already assigned to this chromosome, including both microsatellites and ESTs, was used to construct a genetic map. eibi1 co-segregated with barley EST AV918546, and was located to bin 6. The synteny between barley and rice ill this region is incomplete, with a large discrepancy in map distances, and the presence Of Multiple inversions. No potential conflict of interest relevant to this article was reported.