Amphiploid Seed Obtained by Colchicine Treatment of an F1 Hybrid Between Triticum spelta and Agropyron intermedium

An F1 hybrid plant obtained from a combination that Triticum spelta var. duhamelianum as female crossed with Agropyron intermedium as male was treated with colchicine solution with DMSO as described by WINKLE and KIMBER in 1976. Despite the treatment made under relatively low temperature of early spring, effectiveness of the treatment is evident, because two out of ten plants had a spike with sector of doubled chromosome and several good seeds were obtained from one spike. Also the result indicated that the self-compatibility of the wheat parent is epistatic to the self-imcompatibility of Ag. intermedium.