I. Some fundamental conditions and procedures on filter-paper electrophoresis were investigated. The electrophoretic apparatus of Natsume Seisakusho and Toyo No. 51 filter-paper were used. Electrophoresis was carried out under the current of 0.25 mA/cm. in 300 Volt during 5 hours, using veronal buffer pH 8.6 (μ=0.05). In
measuring of serum protein fractions, the adequate volume of the materials loaded is in the range of 0.005～0.01 ml. and in the case of lipoprotein, 0.02 ml. of serum is used. Paper-strip was stained with bromophenol blue or sudan black B, then making it transparent with heat-solved paraffin and photometered by Nataume's
densitometer. The reproducibility of the above-mentioned method with a confidence of 99% was as follows:
Serum protein fractions
Albumin 53.4±1.8%
α(1)-Globulin 3.3±0.3
α(2)-Globulin 6.0±0.5
β-Globulin 12.2±0.7
γ-Globulin 25.1±1.1
Serum lipoprotein fractions
α 20.6±2.6%
β+γ 79.4±2.6
II. Changes in serum protein fractions and lipoprotein fractions following the radioactive hot spring bathing were as follows: 1). In rabbits, the albumin-fraction of serum protein increased after a series of baths in radioactive hot spring, and the β- and γ-globulin-fractions decreased. 2). By cholesterol-feeding, the albumin-fraction of serum protein of rabbits decreased and the β- and γ-globulin-fractions increased significantly but when a series of baths was carried out in cholesterol-fed rabbits the decrease in the albumin-fraction was slighter than the former. 3). α-globulin-fraction of serum lipoprotein increased after single bathing in
radioactive hot spring and (β+γ) -globulin-fractions showed an inverse change, but the changes of lipoprotein-fractions following a series of baths were not remarkable. 4). By cholesterol-feeding, the ratio of lipoprotein-fractions (β+γ/α) increased, but the ratio tended to decrease following a series of baths in radioactive hot spring.
