A permanent cell line, MT-TY, has been successfully established in continuous culture from a metastatic lymph node of a patient with malignant thymoma (epithelial type). Most of the MT-TY cells grew in suspension with formation of cell clumps. The doubling time of the cells was 36 hours and the modal chromosome number was 68. The MT-TY line is considered to have originated from donor's thymoma cells on the basis of cytogenetic, morphological and chromosomal features.