Author devised a simple Donaggio-Sato Reaction using a hemo aglutination board instead of the test-tube, and obtained the following results. 1) Value obtained from Donaggio Reaction and that from this devised method had a good relation each other. 2) This Reaction method was confirmed to be utilized enough well to all members of a mass examination. 3) Statistically significant increase in. both Donaggio Reaction value and α1-acid glycoprotein in the urine of students just after their routine exercise of running suggested that Donaggio Reaction positive substance and α1-glycoprotein have an intimate relationship between them.