カドミウムの生体作用に対する実験的研究 第1報 ラット肝ミトコンドリアのヌクレオタイドに対するカドミウムの作用,及びK(+)-遊出に対するカドミウムとランタンの相互作用

The influence of cadmium (Cd(2+)) on adenine nucleotide concentration in a reaction mixture consisting of rat liver mitochondria and ADP was studied using high performance liquid chromatography. The results were as follows: An increase in AMP concentration was brought about by the addition of Cd(2+). This increase in AMP concentration occurred in parallel with increase in Cd(2+) concentration. The appearance of AMP was thought to be due to an ATPase reaction and an adenylate kinase reaction stimulated by Cd(2+) although further studies on the adenylate kinase reaction stimulated by Cd(2+) are needed. An increase in AMP may influence metabolism via glycolysis. As lanthanium (La(3+)) is related to Ca(2+)-binding sites, the effect of La(3+) on the K(+)-release of rat liver mitochondria induced by Cd(2+) was also studied. The results were: K(+)-release induced by Cd(2+) was inhibited by La(3+). The Cd(2+)-binding site was thought to be related to the Ca(2+) carrier of mitochondria.