C(3)Hマウス乳癌におけるウイルス抗原の螢光抗体法による研究 第1編 C(3)Hマウスにおける乳癌ウイルス抗原の精製とその螢光抗体法直接法による検討

Virus preparations were obtained from mammary tumors which developed spontaneously in C(3)H mice by three different methods; 1) filtration through Berkefeld-N or Chamberland L(3) filters, 2) differential centrifugations, and 3) fluorocarbou extraction. Each virus preparation was used for immunizing rabbits, and was then evaluated for the degree of purification of the viral antigens by direct immunofluorescent staining. Following results were obtained: 1. The fluorocarbon extractin method was more excellent for the purification of mammary cancer virus than filtration or differential centrifugation method. 2. Staining procedure of the direct immunofluorescence was improved as follows; frozen sections were fixed with cold acetone-fluorocarbon mixture instead of coid acetone, and duration of staining was prolongel over six hours in a cold moist chamber. As a result it was possible to observe clearer and more numerous fluorescence than by the direct staining of Coons' original procedure.