With the aid of bicycle ergometer physiological changes, urinary fatigue reactions and flicker values were estimated and their values were compared on group A given the work on a bicycle with a load of 50kg at 90r.p.m. for 10 minutes and group B with the same load but 60r.p.m. for 30 minutes, and the following results were obtained. 1) The number of respiration and the volume of expiration increased very rapidly in group A, reaching their maximum values after the commencement of the work and thereafter these values decreased up to the cessation of the work due to the functional decrease. Group B maintained these values steadily at a fixed point and the recovery from the physical change was more rapid as compared with group A. The increase in blood pressure and number of pulsation in group A was considerably more marked than that in group B. 2) In group A the urinary fatigue reaetions showed a marked increase in all of Donaggio, Shinmei, mercuric iodide, and urinary protein reactions after the start of work, and these values recovered back to the values before the work four hours after stopping the work. The increase in the fatigue reactions after the work is relatively less in group B. Urinary fatigue reactions in both group A and group B are high immediately after the work, reaching their maximum 30 minutes after the cessation of the work. The values of mercuric iodide reaction alone, however, are at the maximum immediately after the work. 3) The rate of decrease in the flicker values reaches its maximum immediately after the work, and the recovery thereafter is rapid. returning the pre-work values 30 minutes after the work. 4) From these results it appears that for the appearance of urinary fatigue reactions the load factor in a given time has a greater influence than the working-time factor and that these reactions appear when there exists a certain imbalance (for an example, acidosis) in vivo brought about by physiological changes and they appear 30 minutes later rather than immediately after the work.