発育電位時間曲線によるチフス菌代謝の研究 第2編 各基質における酵素促進剤及び阻害剤の電位に及ぼす影響

In the present part, the influence of various enzyme-activators and -inhibitors on oxidation-reduction potential was studied. The salt solution (pH 7.2) was used as the fundamental culture medium; Salmonella typhi 57 S as the test organism. The results were as follows: 1) In the medium of glucose or pyruvate Mg(++) of the concentration over 10(-4)M accelerated the fall of potential. 2) 10(-2)M KCN inhibited the fall of potential in the media of all the substrates tested, whereas 10(-3)M inhibited it in those of the substrates other than pyruvate, succinate, aspartate and alanine, and 10(-4) M inhibited only in that of lactate. 3) NaF of the concentrations over 10(-3) M inhibited the fall of potential in the media of the substrates other than lactate, acetate and succinate. 10(-1) M NaF, however, showed some inhibition even in that of succinate. 4) NaN(3) of all concentrations inhibited the fall of potential in the media of all the substrates other than lactate, acetate and succinate. However, 10(-1)M in the medium of lactate, 10(-3) M in that of acetate, 10(-2) M in that of succinate inhibited the fall of potential. 5) Monoiodoacetic acid of the concentrations over 10(-3) M inhibited the fall of potential in the medium of lactate, that over 10(-4) M inhibited it in that of succinate. 6) 2, 4-Dinitrophenol of all the concentrations tested inhibited the fall of potential in the media of all the substrates tested. 7) The inhibitive action of these five sorts of inhibitors to the fall of potential resembled their action to the oxygen consumption of Salmonella typhi.