The author studied experimentally with the urine, cerebrospinal fluid, and extracts of bone marrow, spleen and the liver and obtained the results as follows; 1) In the urine, there is certain anemia-producing substance which is soluble in alcohol as well as ether, and exists in acid fraction. 2) In the cerebrospinal fluid, some anemia-producing substance could be discovered. 3) The bone marrow, the spleen and the liver have certain anemia-producing substance in their extracts.