岡山県に発生せる流行性肝炎病原体の研究特に孵化鶏卵に依り分離せるウイルスに就て 第2編 分離ウイルスの性状に就て

The author studied the virological characters of the virus isolated by the Chick-embryo technic, and obtained the following results: Inoculation of the virus into the chorio-allantoic cavity of the 7 day old chick-embryo killed the chick-embryo on the 5th to 7th day after inoculation. The serial passage could be carried out by inoculation of the chorio-allantoic fluid or the chick-embryo liver, of which the latter gave a better result. The amniotic inoculation could kill the chick-embryo on the 4th to 5th day after inoculation, but LD(50) was lower than that by the chorio-allantoic route. The yalk sack inoculation could not give a good result. As for the distribution of the virus by chorio-allantoic inoculation, the virus could be best proved in the chick-embryo, particularly in the liver, the next in the chorio-allantoic fluid and very few in the other parts. The isolated virus easily passed Seitz E. K., Berkefeld N. and also Berkefeld W., though somewhat difficult through the last one; this fact suggests that the isolated virus is pretty small one. Though the infectious index of the virus to the chick-embryo varied with the generations of the passage, the average for 10 to 20 generations was 8.0. The isolated virus could be well preserved in the glycerin-salt solution.