result 47501 件
| Title Alternative | シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 |
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| FullText URL | 005_002_145_153.pdf |
| Author | Sugimoto, Manabu| Kawai, Fusako| |
| Abstract | A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. |
| Keywords | Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 145 |
| End Page | 153 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
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| FullText URL | 005_002_135_144.pdf |
| Author | Konno, Haruyoshi| Tsumuki, Hisaaki| Yamasaki, Yoshiki| |
| Abstract | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| Keywords | Cell wall Oryza sativa Pectic polymer |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 135 |
| End Page | 144 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Secretion of Defensive Substance by Carabidae and Brachinidae |
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| FullText URL | 004_001_009_023.pdf |
| Author | Kanehisa, Katsuo| |
| Abstract | The secretion of defensive substances by more than 250 species of carabid and brachinid beetles was investigated, and discussed from a phylogenetical viewpoint. The morphological of secretion glands and secreted components were identified in the genus level. Specific subgenus and species had peculiar characteristics. The pair of defensive organs consisted of many small synthetic lobes, large reservoirs, collecting ducts from lobes to reservoirs and secretion ducts from reservoirs. The pair dust opened at the last sternal intersegmental membrane. There was species specificity in the reservoir shape, synthetic lobe shape and entering site of the collecting ducts into reservoirs. The spherical shape lobes produced short chain fatty acids, the elongated shape lobes produced m-cresol and the thick board shape lobes produced benzoquinones. Two types of fatty acid-secreting beetles were found, one secreted formic acid, and the other mixed short chain fatty acids. These short chain fatty acids were metabolized from some amino acids, methacrylic acid was from valine; tiglic acid, α-ethyacrylic acid and angelic acid were from isoleucine; senecioic acid was from leucine and crotonic acid was from lysine. These unasaturated acids were sometimes detected with a trace amount of corresponding saturated acids. A few species secreted larger amounts of saturated acids with a trace amounts of unsaturated acids. Fatty acid composition was generally genus-specific. In a few cases, however, species-specific acid composition was also observed. m-Cresol-secreting beetles were observed only in Panagaeni and Chlaeniini except subgenus Chlaenius Chlaeniini except subgenus Chlaenius Chlaeniellus beetle. 1,4-Benzoquinone and 2-methyl-1,4-benzoquinone-secreting beetles were observed in subgenus C. Chlaeniellus. This beetle secreted these substances at body temperature from a reservoir with no sound. Brachinidae beetles, called bombardier beetles, have a pair of brownish reaction chamber connected with a reservoir. They sprayed the two benzoquinones at about 100℃ making sound. |
| Keywords | Defensive secreting substance Synthetic organ Carabidae Brachinidae Phylogenetic relation |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 23 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Purification and Properties of α-Glucodidase from Taro Tuber |
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| FullText URL | 005_002_129_134.pdf |
| Author | Mashima, Hideyuki| Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. |
| Keywords | α-glucosidase taro tuber Colocasia esculanta Shott |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 129 |
| End Page | 134 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | ナメクジのα-グルコシダーゼの精製と性質 |
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| FullText URL | 005_002_121_127.pdf |
| Author | Yamasaki, Yoshiki| Konno, Haruyoshi| |
| Abstract | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| Keywords | Slug Incilaria bilineata α-glucosidase |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1998 |
| Volume | volume5 |
| Issue | issue2 |
| Start Page | 121 |
| End Page | 127 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Varietal Variation and Effects of Some Major Genes on Salt Tolerance in Barley Seedlins |
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| FullText URL | 003_001_071_081.pdf |
| Author | Mano, Yoshiro| Takeda, Kazuyoshi| |
| Abstract | In order to select the salt tolerant cultivars, and to evaluate the effects of some major genes on salt tolerance in barley, 5,182 varieties and 368 isogenic lines were tested by exposing to 1.0 and 0.5M sodium chloride at the seedling stage. The average rating of salt tolerance was higher in varieties from Korea and Japan , but lower in varieties from Nepal and Southwest Asia. When the varieties were grouped by several morphological characters, six-rowed, covered, E type of nonbrittle rachis and uzu type groups were more tolerant than two-rowed, naked, W type and non-uzu type counterparts, respectively. A comparison between the isogenic pairs revealed that the uzu type was more tolerant than non-uzu type, but other major gene pairs did not show any obvious differences. These findings indicate that the uz gene affects the salt tolerance, and diferences between varietal groups characterized by other major genes are due to the difference in the genetic background among these varieties. |
| Keywords | Barley Seedling Salt tolerance Screening Isogenic line |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 71 |
| End Page | 81 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Diallel Analysis of 1,000-grain Weight in Two-rowed Barley Varieties |
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| FullText URL | 003_001_063_070.pdf |
| Author | Wu, Jiri| Takeda, Kazuyoshi| |
| Abstract | The inheritance of 1,000-grain weight of two-rowed barley varieties was examined using a reciprocal F1 cross and a half F2 diallel cross among eight parents. The F1 generation was examined in five different ripening conditions i.e., (Ⅰ) control, (Ⅱ) leaf cut, in which all leaves were cut at the heading time, (Ⅲ) Spikelet thinned, in which a quarter of the spikelets were thinned at the heading time, (Ⅳ) dry, in which the plants were grown in a vinyl-film house without irrigation, and (Ⅴ) wet, in which the plants were mist-irrigated every two house in the daytime after the heading to the harvest. The experiment was repeated twice. The F2 populations were grown in the ordinary condition. The average 1,000-grain weight was largest in the dry plot followed by spikelet thinned, control, leaf cut, and wet plots. The 1,000-grain weight was predominantly controlled by the additive genes. The dominance effect was also significant. The large grain weight was controlled by the overdominant genes. Epistasis among the genes controlling the 1,000-grain weight was significant, but the maternal effect was absent. In the F1 diallel, heritability was 0.3~0.6 and 0.2~0.5 in a broad and a narrow sense, respectively. In the F2 diallel, it was 0.9 and 0.6 in a broad and a narrow sense, respectively. In each of the 28 F2 populations, the broad sense heritability was estimated as 0.3~0.8. |
| Keywords | Barley Grain size Diallel analysis |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 63 |
| End Page | 70 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Purification and Characterization of α-Glucosidases from Spinach Seeds |
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| FullText URL | 004_002_239_252.pdf |
| Author | Sugimoto, Manabu| Furui, Satoshi| Suzuki, Yukio| |
| Abstract | Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Ⅰand Ⅱ showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases Ⅲ and Ⅳ showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase Ⅲ serum precipitated specifically with α-glucosidase Ⅲ. |
| Keywords | α-Glucosidase Spinach Seed Spinacia oleracea L. Molecular form |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 239 |
| End Page | 252 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Production of Strain of Erysiphe graminis f. sp. tritici That Are Pathogenic on Barley and Agropyron |
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| FullText URL | 004_002_229_237.pdf |
| Author | Heta, Hideo| Inouye, Narinobu| |
| Abstract | Two isolates of Erysiphe graminis f. sp. tritici, T3 and T4, were crossed and selected for pathogenicity. The corssing and selection process were repeated five times with the progenies. Isolates of each generation were inoculated on the non-hosts, Hordeum vulgare and Agropyron tsukushiense var. transiens OHWI, and parasitism and pathogenicity were investigated with respect to host specificity.As shown in Tables 2 to 5, the process of crossing and selection produced isolates which were pathogenic on barley cultivars, 'Kagoshimahadaka' or 'Russian 9' or Agropyron tsukushiense ecotypes 'Agropyron Early ecotype' or 'Agropyron Yezoense 3'.Factors affecting the degree of pathogenicity appear to be under polygenic control. The cumulative effect of minor genes may play a role in the differentiation of formae speciales suggesting a new concept of pathogenicity in the powdery mildew of grasses. |
| Keywords | Wheat powdery mildew Host specific Pathogenicity Properties Barley Agropyron |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 229 |
| End Page | 237 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Comparison between shoot Regeneration Ability in Calli Derived from Immature Embryos of Wild and Cultivated Barley |
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| FullText URL | 003_001_055_062.pdf |
| Author | Rikiishi, Kazuhide| Takeda, Kazuyoshi| Yasuda, Shozo| |
| Abstract | The variation in shoot regeneration obility of calli derived from immature embryos was examined in 95 wild strains, 82 of which were of Hordeum spontaneum and 13 of which were H. agriocrithon, and 87 cultivated varieties collected from various countries or regions of the world. In 85 strains of the wild species, a number of calli regenerated shoots, and their proportion ranged from 1.2% to 75.7%. The average percentage of shoot regenerating calli was 21.7% among the strains that formed calli, 11.5% of which regenerated green and 10.2% albino shoots. On average, 21.4% and 23.9% calli regenerated shoots in H. spontaneum and H. agriocrithon, respectively and there was no significant difference between these values. A significant difference in the percentage of shoot regenerating calli was found among six variants (dawense, ishnatherum, laguncliforme, paradoxon, proskowetzii, spontaneum) which were comprised in H. spontaneum. In 73 varieties of cultivated species, there were shoots regenerating calli likely to wild species, and their proportion ranged from 3.2% to 85.5%. The average percentage of shoot regenerating calli was 25.4%, 22.0% of which regenerated green and 3.4% of which regenerated albino shoots. There was a significant difference in percentage of green shoots regenerating calli against shoots regenerating ones between the wild (53.0%) and cultivated species 886.6%). The two kinds of non-brittle rachis genotypes, Bt bt2 and bt Bt2 are one of the key characters distinguishing the oriental and occidental types of cultivated barley. The average percentages of shoot regenerating calli were 16.2% and 32.3% for the genotypes Bt bt2 and bt Bt2, respectively, suggesting that there is a geographical variation in the shoot regeneration ability of calli in the cultivated species. By contrast, the oriental and occidental strains of wild species showed no difference in the shoot regeneration ability of calli. The geographical variation of shoot regeneration ability differed significantly between wild and cultivated species. This suggests that the geographical variation of shoot regeneration ability occurred after the cultivation of the barley was established. |
| Keywords | Hordeum spontaneum Hordeum agriocrithon Tissue culture Geographical variation Shoot regeneration ability |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 55 |
| End Page | 62 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Author | Oshiki, Toshiyuki| |
|---|---|
| Published Date | 2007-03-24 |
| Publication Title | |
| Content Type | Conference Paper |
| Title Alternative | Some Properties of Azuki Bean Mosaic Virus Isolated from Soybean Plants in Ibaraki Prefecture |
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| FullText URL | 004_002_215_227.pdf |
| Author | Takahashi, Kokichi| Inouye, Narinobu| Maeda, Takanori| Mitsuhata, Koji| Chida, Shigeki| Takahashi, Yoshiyuki| |
| Abstract | Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40～50% less than that of healthy plants. |
| Keywords | Potyvirus Azuki bean mosaic virus Glycine max Properties |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 215 |
| End Page | 227 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | 大麦網斑病抵抗性の選抜効果 |
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| FullText URL | 003_001_043_053.pdf |
| Author | Sato, Kazuhiro| Takeda, Kazuyoshi| |
| Abstract | Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. |
| Keywords | Net blotch Pyrenophora teres Selection Barley Disease resistance |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 43 |
| End Page | 53 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120004537480 |
| Title Alternative | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
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| FullText URL | 003_001_035_041.pdf |
| Author | Hou, Fwu-Fenn| Thseng, Fu-Sheng| Wu, Shu-Tu| Takeda, Kazuyoshi| |
| Abstract | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| Keywords | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 35 |
| End Page | 41 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| Title Alternative | Ixia から分離された bean yellow mosaic virus |
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| FullText URL | 004_002_201_213.pdf |
| Author | Tsuji, Toshiya| Maeda, Takanori| Kondo, Hideki| Inouye, Narinobu| |
| Abstract | A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. |
| Keywords | Ixia hybrida Bean yellow mosaic virus Potyvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 201 |
| End Page | 213 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313395 |
| Title Alternative | Sorghum Resistance to the Sugarcane Aphid, Melanaphis sacchari (Zehntenr) Amounts of Surface Wax and Nutritional Components |
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| FullText URL | 003_001_027_034.pdf |
| Author | Tsumuki, Hisaaki| Kanehisa, Katsuo| Moharramipour, Saeid| |
| Abstract | The differences in the amounts of leaf surface wax and nutritional components such as sugar and free amino acids were compared among resistant (PE 954177), moderately resistant (IS 84) and susceptible (Redlan B) sorghum varieties to the sugarcane aphid. Melanaphis sacchari (Zehntner). (1) The kind of aphid appearing on the leaves of the sorghum varieties was only the sugarcane aphid, Melanaphis sacchari in the field. The number of aphids infesting a leaf was ca 0,30 and 3,500 in the resistant, moderately resistant and susceptible varieties at the middle of August, respectively. (2) The amount of leaf surface wax was approximately constant among these three varieties. (3) The total sugar content was slightly higher in the two resistant varieties than in the susceptible variety. (4) The total free amino acid concentration was higher in the two resistant varieties than in the susceptible variety. (5) These findings suggest that neither leaf surface wax nor nutritional components seem to affect the aphid populations on the leaves of the three sorghum varieties, showing that biogenic substances such as dhurrin, benzylalcohol, p-hydroxybenzaldehyde, aconitic acide, etc. may affect the aphid populations. |
| Keywords | Sorghum Sugarcane aphid Melanaphis sacchari Leaf surface wax Amino acid |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 27 |
| End Page | 34 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Some Properties of Cymbidium Mosaic Virus Isolated from Calanthe spp. |
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| FullText URL | 004_002_187_199.pdf |
| Author | Matsumoto, Jun-ichi| Urabe, Shinji| Maeda, Takanori| Mitsuhata, Koji| Kondo, Hideki| Tahara, Mochimu| Inouye, Narinobu| |
| Abstract | Cymbidium mosaic virus(CyMV) was isolated from Calanthe spp. showing mosaic on the leaves, collected in Yamaguchi and Kyoto Prefectures in 1986～1993. CyMV, Cal. 90-1 isolate was transmitted by sapinoculation to 12 out of 37 species in 7 out of 9 families. Sap from diseaded Tetragonia expansa was infective to Chenopodium amaranticolor after dilution to 10-5 but not 10-6, after heating at 65℃ for 10 min but not 70℃, and after 1 month at 20℃ but not 2 months. The virus particles were flexuous rod, about 475 nm long. The virus was purified from diseased T. expansa leaves and contained a single protein species of Mr27,800. The Mr of the capsid proteins(Cal. 90-1) was similar to those of two ohter CyMV isolates(Cal. 90-4, Cal. 93-14).Cal. 90-1 and Cal. 93-14 reacted with antiserum to the Cymbidium isolate (Cy-16), suggesting that Cal. 90-1 was serologically very similar to the other two CyMV isolates. Two species of dsRNA were isolated from plants infected with Cal-1 and they were similar to those of two other CyMv isolates. |
| Keywords | Calanthe spp. Cymbidium mosaic virus Potexvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 187 |
| End Page | 199 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313956 |
| Title Alternative | Close Relationship between Serotype and Pathogenicity of Cucumber Mosaic Virus |
|---|---|
| FullText URL | 004_002_175_185.pdf |
| Author | Maeda, Takanori| Mitsuhata, Koji| Inouye, Narinobu| |
| Abstract | Sixty-eight isolates of cucumber mosaic virus (CMV) belonging to the Y serotype (serogroup) or P serotype (serotype Ⅱ) in Japan were inoculated to Nicotiana tabacum cv. White Burley and the symptoms were observed up to three months after inoculation. These isolates were divided into four goups accoding to symptomatology on tabacco plant. None of the isoletes obtained from lilies infected tobacco plants systemically. The plants inoculated with isolates of the P serotype showed systemic mosaic, but only occasional mild symptoms were observed on newly developed leaves. On the other hand, all isolates of Y serotype showed symptoms consisting of mosaic, distortion and/or necrosis throughout the experimental period. In another experiment, reactions of some selected test plants to 30 isolates belonging to the Y or P serotype were examined. Lily isolates may be unique strain of CMV as they did not cause systemic infections on Lycopersicon esculentum, Solanum melongena or Cucumis sativus. Host range tests using 16 isolates originally obtained from plants other than lilies demonstrated a close relationship between serotype and pathogenicity to some test plants including N.tabacum, N. clevelandii, L.esculentum, Pisum sativum, phaseolus angularis and Zea mays. |
| Keywords | Cucumber mosaic virus Serotype Pathogenicity |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 175 |
| End Page | 185 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Quantitative Variations of a Resistance Substance, DIMBOA, against Aphids in Wheat Varieties. |
|---|---|
| FullText URL | 003_001_017_026.pdf |
| Author | Kanehisa, Katsuo| Awan Rustamani, Maqsood| Cheng, Wen-Yi| Tsumuki, Hisaaki| Shiraga, Takashi| |
| Abstract | Aphids sometimes severely infest wheat plants, mainly sucking phloem sap and disrupting tissues, and in a few cases act as virus vectors. There are resistant and susceptible varieties of wheat against aphids. DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) is a substance causing resistance to animals including aphids. The change in the amounts of DIMBOA with growth in eight wheat varieties was estimated in 1990 and 1991. Wheat seeds were sown at mid-November in the former year and harvested in mid-June. Aphids appeared from early April, increased with the growth of the wheat, and finally decreased with the senescence of the wheat. Rhopalosiphum maidis appeared early in the season, R. padi appeared late, Schizaphis graminum and Sitovion akebiae appeared intermittently in the season. S. graminum appeared more frequently on wheat than barley. DIMBOA was detected from wheat but not from barley. Gramine (N,N-dimethyl-indole-3-methanamine) was detected from barley, and is known as an important resistance substance. However gramine could not be detected in wheat. DIMBOA was found in higher amounts in young wheat, and gradually decreased with growth. A clear relationship between the aphid population and DIMBOA amounts could not be observed. However, all the wheat varieties used in this experiment seemed to have resistance against aphids. The resistance was compared with barley susceptible lines. DIMBOA was presumed to share the property of resistance with aconitic acid in wheat. |
| Keywords | DIMBOA content Wheat Aphid Resistant varaity Susceptible variety |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1995 |
| Volume | volume3 |
| Issue | issue1 |
| Start Page | 17 |
| End Page | 26 |
| ISSN | 0916-930X |
| language | Japanese |
| File Version | publisher |
| Title Alternative | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
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| FullText URL | 004_002_164_174.pdf |
| Author | Gara, I Wayan| Kondo, Hideki| Maeda, Takanori| Mitsuhata, Koji| Inouye, Narinobu| |
| Abstract | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| Keywords | Vanda orchid Cymbidium mosaic virus Potexvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 164 |
| End Page | 174 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313836 |
