result 1019 件
| JaLCDOI | 10.18926/AMO/56860 |
|---|---|
| FullText URL | 73_3_189.pdf |
| Author | Sakamoto, Shinji| Kawai, Hiroki| Okahisa, Yuko| Tsutsui, Ko| Kanbayashi, Takashi| Tanaka, Keiko| Mizuki, Yutaka| Takaki, Manabu| Yamada, Norihito| |
| Abstract | Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a recently-discovered autoimmune disorder in which antibodies target NMDAR in the brain. The number of reported cases of anti-NMDAR encephalitis has increased rapidly. Anti-NMDAR encephalitis can be mistakenly diagnosed as psychiatric disorders because many patients present with prominent psychiatric symptoms and visit psychiatric institutions first. Thus, psychiatrists should cultivate a better understanding of anti-NMDAR encephalitis. In this review, we present the mechanisms, epidemiology, symptoms and clinical course, diagnostic tests, treatment and outcomes of patients with anti-NMDAR encephalitis. Furthermore, we discuss the diversity of clinical spectra of anti-NMDAR encephalitis, and demonstrate a differential diagnosis of psychiatric disease from the perspective of psychiatry. |
| Keywords | NMDAR encephalitis psychiatric symptom schizophrenia mood disorder |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2019-06 |
| Volume | volume73 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 189 |
| End Page | 195 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2019 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 31235965 |
| FullText URL | K0005980_abstract_review.pdf K0005980_fulltext.pdf |
|---|---|
| Author | Zhou, Xiaoyu| |
| Published Date | 2019-03-25 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5980号 |
| Granted Date | 2019-03-25 |
| Thesis Type | Doctor of Philosophy in Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | ClinMicrobiolInfect_19_2_173.pdf |
|---|---|
| Author | Sinha, A.| SenGupta, S.| Guin, S.| Dutta, S.| Ghosh, S.| Mukherjee, P.| Mukhopadhyay, A. K.| Ramamurthy, T.| Takeda, Y.| Kurakawa, T.| Nomoto, K.| Nair, G. B.| Nandy, R. K.| |
| Keywords | Real-time PCR Diarrhoea Polymicrobial infection |
| Note | This is an Accepted Manuscript of an article published by Elsevier B.V| |
| Published Date | 2013-02 |
| Publication Title | Clinical Microbiology and Infection |
| Volume | volume19 |
| Issue | issue2 |
| Publisher | Elsevier B.V |
| Start Page | 173 |
| End Page | 180 |
| ISSN | 1198743X |
| NCID | AA11098239 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 22268636 |
| DOI | 10.1111/j.1469-0691.2011.03746.x |
| Web of Science KeyUT | 000314656100019 |
| Related Url | isVersionOf https://doi.org/10.1111/j.1469-0691.2011.03746.x |
| FullText URL | J_ClinMicrobiol_51_3_1040.pdf |
|---|---|
| Author | Naha, A.| Chowdhury, G.| Ghosh-Banerjee, J.| Senoh, M.| Takahashi, T.| Ley, B.| Thriemer, K.| Deen, J.| Seidlein, L. V.| Ali, S. M.| Khatib, A.| Ramamurthy, T.| Nandy, R. K.| Nair, G. B.| Takeda, Y.| Mukhopadhyay, A. K.| |
| Keywords | Vibrio cholerae ctxB Cholera PFGE |
| Note | This is an Accepted Manuscript of an article published by American Society for Microbiology| |
| Published Date | 2013-03 |
| Publication Title | Journal of Clinical Microbiology |
| Volume | volume51 |
| Issue | issue3 |
| Publisher | American Society for Microbiology |
| Start Page | 1040 |
| End Page | 1045 |
| ISSN | 00951137 |
| NCID | AA00695531 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 23325815 |
| DOI | 10.1128/JCM.03162-12 |
| Web of Science KeyUT | 000315121700055 |
| Related Url | isVersionOf https://doi.org/10.1128/JCM.03162-12 |
| JaLCDOI | 10.18926/ESR/56695 |
|---|---|
| FullText URL | esr_025_039_048.pdf |
| Author | Knittel, Ulrich| Walia, Monika| Suzuki, Shigeyuki| Lee, Yuan-Hsi| |
| Abstract | The high-P/low-T Sanbagawa Metamorphic Belt that traverses SW Japan, has been subdivided into two belts thought to have been metamorphosed at ca. 120 Ma and at ca. 65 Ma (‘Sanbagawa Metamorphic Rocks’ and ‘Shimanto Metamorphic Rocks’). The subdivision was based on the assumption that metamorphism occurred at ca. 116 Ma, largely based on an early Rb-Sr isotope study and zircon data obtained for the eclogite unit of the Sanbagawa Belt, whereas in some parts of the belt detrital zircons of late Cretaceous age (90-80 Ma) were discovered. Analysis of detrital zircons sampled from two sites within the area considered to expose the older ‘Sanbagawa Metamorphic Rocks’, including the area investigated by the Rb-Sr study, reveals the presence of zircons younger than 95 Ma in all samples and some grains as young as 80 ± 4 Ma. It is therefore concluded that the Sanbagawa Belt is one single tectonic entity that formed in the Late Cretaceous though it contains older components, including fossiliferous clasts, older basic meta-volcanics and eclogite units that may record earlier metamorphic events. |
| Keywords | U-Pb zircon dating Sanbagawa Metamorphic Belt Late Cretaceous Asemi River |
| Publication Title | Okayama University Earth Science Report |
| Published Date | 2018-12-27 |
| Volume | volume25 |
| Issue | issue1 |
| Start Page | 39 |
| End Page | 48 |
| ISSN | 1340-7414 |
| language | English |
| Copyright Holders | © 2018 by Okayama University Earth Science Reports Editorial Committee All Rights Reserved |
| File Version | publisher |
| FullText URL | Foodborne_Pathog_Dis_10_10_904.pdf |
|---|---|
| Author | Chowdhury, Goutam| Sarkar, Anirban| Pazhani, Gururaja P.| Mukhopadhyay, Asish K.| Bhattacharya, Mihir K.| Ramamurthy, Thandavarayan| |
| Keywords | Diarrhoea S. Weltevredan V. fluvialis |
| Note | This is an Accepted Manuscript of an article published by MARY ANN LIEBERT| |
| Published Date | 2013-09-25 |
| Publication Title | Foodborne Pathogens and Disease |
| Volume | volume10 |
| Issue | issue10 |
| Publisher | MARY ANN LIEBERT, INC |
| Start Page | 904 |
| End Page | 906 |
| ISSN | 15353141 |
| NCID | AA11691645 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 23789767 |
| DOI | 10.1089/fpd.2013.1491 |
| Web of Science KeyUT | 000324835400012 |
| Related Url | isVersionOf https://doi.org/10.1089/fpd.2013.1491 |
| FullText URL | GutPathog_5_1_36.pdf |
|---|---|
| Author | Dhira Rani Saha| Guin, Sucharita| Krishnan, Rajendran| Nag, Dhrubajyoti| Koley, Hemanta| Shinoda, Sumio| Ramamurthy, Thandavarayan| |
| Published Date | 2013-12-03 |
| Publication Title | Gut Pathogens |
| Volume | volume5 |
| Issue | issue1 |
| Publisher | BioMed Central |
| Start Page | 36 |
| ISSN | 17574749 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| PubMed ID | 24294997 |
| DOI | 10.1186/1757-4749-5-36 |
| Web of Science KeyUT | 000328551200001 |
| Related Url | isVersionOf https://doi.org/10.1186/1757-4749-5-36 |
| FullText URL | BriMicroRes_Jou_3_2_190.pdf |
|---|---|
| Author | Mukherjee, Avik Kumar| Karmakar, Sumallya| Raj, Dibyendu| Ganguly, Sandipan| |
| Keywords | Giardia genotyping mixed assemblages local isolates |
| Published Date | 2013-03-22 |
| Publication Title | British Microbiology Research Journal |
| Volume | volume3 |
| Issue | issue2 |
| Publisher | SCIENCEDOMAIN international |
| Start Page | 190 |
| End Page | 197 |
| ISSN | 22310886 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| DOI | 10.9734/BMRJ/2013/2812 |
| Related Url | isVersionOf https://doi.org/10.9734/BMRJ/2013/2812 |
| FullText URL | Vaccine_32_A20-8.pdf |
|---|---|
| Author | Mullick, Satarupa| Mandal, Paulami| Mukti Kant Nayak| Ghosh, Souvik| De, Papiya| Rajendran, K.| Bhattacharya, Mihir K.| Mitra, Utpala| Ramamurthy, Thandavarayan| Kobayashi, Nobumichi| Chawla-Sarkar, Mamta| |
| Keywords | Diarrhea Rotavirus India Kolkata G9 strains G2 strains |
| Published Date | 2014-08-11 |
| Publication Title | Vaccine |
| Volume | volume32 |
| Issue | issuesupplment 1 |
| Publisher | Elsevier Science |
| Start Page | A20 |
| End Page | A28 |
| ISSN | 0264410X |
| NCID | AA10491877 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| PubMed ID | 25091674 |
| DOI | 10.1016/j.vaccine.2014.03.018 |
| Web of Science KeyUT | 000340977500007 |
| Related Url | isVersionOf https://doi.org/10.1016/j.vaccine.2014.03.018 |
| FullText URL | Microbiologyopen_3_2_239.pdf |
|---|---|
| Author | Senoh, Mitsutoshi| Ghosh-Banerjee, Jayeeta| Mizuno, Tamaki| Shinoda, Sumio| Miyoshi, Shin-ichi| Hamabata, Takashi| Nair, G. Balakrish| Takeda, Yoshifumi| |
| Keywords | Factor converting VBNC into culturable VBNC Vibrio cholerae |
| Published Date | 2014-02-18 |
| Publication Title | Microbiologyopen |
| Volume | volume3 |
| Issue | issue2 |
| Publisher | Wiley-Blackwell |
| Start Page | 239 |
| End Page | 246 |
| ISSN | 20458827 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| PubMed ID | 24574069 |
| DOI | 10.1002/mbo3.164 |
| Web of Science KeyUT | 000342352400007 |
| Related Url | isVersionOf https://doi.org/10.1002/mbo3.164 |
| JaLCDOI | 10.18926/AMO/56644 |
|---|---|
| FullText URL | 73_2_95.pdf |
| Author | Ercan, Sabriye| Başkurt, Ferdi| Başkurt, Zeliha| Çetin, Cem| |
| Abstract | The aim of this study was to examine effect of fatigue levels on functional mobility and balance in middle-aged obese females. The study included 98 healthy females aged 40-60 years with BMI ≥ 30 kg/m2. Self-perceived fatigue was evaluated with a self-administered questionnaire, the Checklist of Individual Strength in Turkish (CIS-T), on which 62.3% of the study subjects identified themselves as fatigued. The body weight and BMI values of fatigued subjects were found to be higher than those of the non-fatigue group (p<0.05). The reported exercise habits of the fatigue group were found to be significantly lower than their non-fatigued peers (p<0.05). The level of functional mobility (Timed Up and Go Test), static balance (One-Legged Stance Test with Eyes Open) and dynamic balance (Functional Reach Test and the 3-meter Timed Tandem Walk Test) were measured, and statistically significant differences were found between fatigue and non-fatigue groups on all of them (p<0.05). In addition, correlations were found between the sub-parameters of the CIS-T scale and the functional tests at |
| Keywords | fatigue obesity mobility balance woman |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2019-04 |
| Volume | volume73 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 95 |
| End Page | 100 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2019 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 31015743 |
| JaLCDOI | 10.18926/AMO/56456 |
|---|---|
| FullText URL | 73_1_29.pdf |
| Author | Matsumoto, Atsushi| Nakamura, Takehiro| Shinomiya, Aya| Kawakita, Kenya| Kawanishi, Masahiko| Miyake, Keisuke| Kuroda, Yasuhiro| Keep, Richard F.| Tamiya, Takashi| |
| Abstract | Cerebral vasospasm (CVS) is a major contributor to the high morbidity and mortality of aneurysmal subarachnoid hemorrhage (aSAH) patients. We measured histidine-rich glycoprotein (HRG), a new biomarker of aSAH, in cerebrospinal fluid (CSF) to investigate whether HRG might be an early predictor of CVS. A total of seven controls and 14 aSAH patients (8 males, 6 females aged 53.4±15.4 years) were enrolled, and serial CSF and serum samples were taken. We allocated these samples to three phases (T1-T3) and measured HRG, interleukin (IL)-6, fibrinopeptide A (FpA), and 8-hydroxy-2’-deoxyguanosine (8OHdG) in the CSF, and the HRG in serum. We also examined the release of HRG in rat blood incubated in artificial CSF. In contrast to the other biomarkers examined, the change in the CSF HRG concentration was significantly different between the nonspasm and spasm groups (p<0.01). The rat blood/CSF model revealed a time course similar to that of the human CSF samples in the non-spasm group. HRG thus appears to have the potential to become an early predictor of CVS. In addition, the interaction of HRG with IL-6, FpA, and 8OHdG may form the pathology of CVS. |
| Keywords | biomarker histidine-rich glycoprotein predictor subarachnoid hemorrhage vasospasm |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2019-02 |
| Volume | volume73 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 29 |
| End Page | 39 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2019 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 30820052 |
| JaLCDOI | 10.18926/AMO/56452 |
|---|---|
| FullText URL | 73_1_1.pdf |
| Author | Morizane, Shin| |
| Abstract | Excessive protease activity is a characteristic abnormality that affects the epidermal barrier in patients with atopic dermatitis (AD). Kallikrein-related peptidases (KLKs) are excessively expressed in AD lesions, and it is suggested that the abnormal action of KLKs is involved in the skin barrier dysfunction in AD. In other words, overexpressed KLKs disrupt the normal barrier function, and due to that breakdown, external substances that can become antigens of AD easily invade the epidermis, resulting in dermatitis, coupled with the induction of Th2 cytokines. Further investigations are required to elucidate the role of KLKs in AD; this knowledge could contribute to the design of new therapeutic and prophylactic drugs for AD. |
| Keywords | atopic dermatitis kallikrein-related peptidases epidermal barrier dysfunction |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2019-02 |
| Volume | volume73 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2019 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 30820048 |
| FullText URL | PhysRevB_97_064425.pdf |
|---|---|
| Author | Horigane, Kazumasa| Fujii, M.| Okabe, H.| Kobayashi, K.| Horie, R.| Ishii, H.| Liao, Y. F.| Kubozono, Y.| Koda, A.| Kadono, R.| Akimitsu, J.| |
| Note | This is an article published by American Physical Society| |
| Published Date | 2018-02-28 |
| Publication Title | Physical Review B |
| Volume | volume97 |
| Publisher | American Physical Society |
| Start Page | 064425 |
| ISSN | 2469-9950 |
| NCID | AA11187113 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| DOI | 10.1103/PhysRevB.97.064425 |
| Web of Science KeyUT | 000426321000005 |
| Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.97.064425 |
| FullText URL | PhysRevB_97_184510.pdf |
|---|---|
| Author | Maeda, S.| Matano, K.| Zheng, Guo-qing| |
| Note | This is an article published by American Physical Society| |
| Published Date | 2018-05-21 |
| Publication Title | Physical Review B |
| Volume | volume97 |
| Issue | issue18 |
| Start Page | 184510 |
| ISSN | 01631829 |
| NCID | AA11187113 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | ©2018 American Physical Society |
| File Version | publisher |
| DOI | 10.1103/PhysRevB.97.184510 |
| Web of Science KeyUT | 000433012300004 |
| Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.97.184510 |
| JaLCDOI | 10.18926/AMO/56248 |
|---|---|
| FullText URL | 72_5_499.pdf |
| Author | Kodama, Yuya| Furumatsu, Takayuki| Maehara, Ami| Ozaki, Toshifumi| |
| Abstract | Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus. However, little is known about these meniscal clusters in humans. The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci. Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints. The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan. Their signal density after staining was assessed using ImageJ software. Five regions of interest were analyzed within each tissue sample. The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus. In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters. We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries. In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues. |
| Keywords | cell cluster meniscus osteoarthritis Sry-type HMG box 9 type II collagen |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2018-10 |
| Volume | volume72 |
| Issue | issue5 |
| Publisher | Okayama University Medical School |
| Start Page | 499 |
| End Page | 506 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2018 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 30369607 |
| FullText URL | PhysRevB_97_104503.pdf |
|---|---|
| Author | He, Tong| Yang, Xiaofan| Terao, Takahiro| Uchiyama, Takaki| Ueno, Teppei| Kobayashi, Kaya| Akimitsu, Jun| Miyazaki, Takafumi| Nishioka, Takumi| Kimura, Koji| Hayashi, Kouichi| Happo, Naohisa| Yamaoka, Hitoshi| Ishii, Hirofumi| Liao, Yen-Fa| Ota, Hiromi| Goto, Hidenori| Kubozono, Yoshihiro| |
| Note | This is an article published by American Physical Society| This study was partly supported by Grants-in-Aid (Grants No. 26105004 and No. 17K05500) from MEXT, by JST ACT-C Grant No. JPMJCR12YW| |
| Published Date | 2018-03-07 |
| Publication Title | Physical Review B |
| Volume | volume97 |
| Issue | issue10 |
| Publisher | American Physical Society |
| Start Page | 104503 |
| ISSN | 01631829 |
| NCID | AA11187113 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | publisher |
| DOI | 10.1103/PhysRevB.97.104503 |
| Web of Science KeyUT | 000426776800003 |
| Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.97.104503 |
| FullText URL | Experim_Fluid_58_98.pdf |
|---|---|
| Author | Kouchi, Toshinori| Masuya, Goro| Yanase, Shinichiro| |
| Note | This is an Accepted Manuscript of an article published by Springer| |
| Published Date | 2017-07-07 |
| Publication Title | Experiments in Fluids |
| Volume | volume58 |
| Issue | issue8 |
| Publisher | Springer |
| Start Page | 98 |
| ISSN | 07234864 |
| NCID | AA10447762 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| DOI | 10.1007/s00348-017-2377-z |
| Web of Science KeyUT | 000407095300009 |
| Related Url | isVersionOf https://doi.org/10.1007/s00348-017-2377-z |
| JaLCDOI | 10.18926/AMO/56074 |
|---|---|
| FullText URL | 72_3_283.pdf |
| Author | Namba, Shihoko| Ikeda, Fusao| Takaguchi, Koichi| Shimomura, Yasuyuki| Yasunaka, Tetsuya| Okada, Hiroyuki| |
| Abstract | We investigated whether a small amount of blood collected by fingertip blood sampling would be adequate in a mass examination for hepatitis virus infection in Japan. A cross-sectional survey was conducted at health fairs in Kasaoka City and Shodoshima Island, where participants took the hepatitis screening test. A total of 114 consecutive individuals who took the hepatitis screening test were enrolled. Twenty microliters of plasma was successfully obtained from all participants. Among the participants, two had positive results for HBs antigen and two were positive for anti-HCV; all four were > 60 years old and rarely visited the hospital. Thirty-three and 38 patients chronically infected with HBV and HCV, respectively, were examined for confirmatory assays at participating hospitals. All subjects with undetectable serum levels of HBs antigen and anti-HCV had undetectable levels of both markers in fingertip blood, and the levels in serum and fingertip blood were significantly correlated (p<0.01). The lower detection limit of HBs antigen was defined as 0.005 IU/ml, and the cut-off value of anti-HCV was 1.0 by using 10-μl fingertip blood samples. The fingertip blood sampling described herein may be adequate in mass examinations for hepatitis virus testing in Japan. |
| Keywords | fingertip hepatitis test HBV HCV Japan |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2018-06 |
| Volume | volume72 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 283 |
| End Page | 287 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2018 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 29926006 |
| JaLCDOI | 10.18926/AMO/56070 |
|---|---|
| FullText URL | 72_3_249.pdf |
| Author | Nakahara, Takako| Suemori, Shin-ichiro| Tsujioka, Takayuki| Kataoka, Mikio| Kataoka, Hiromi| Shibakura, Misako| Tohyama, Kaoru| |
| Abstract | To investigate megakaryocyte (MK) DNA ploidy in various hematological diseases, fluorescence microscopy imaging system (FMI) can be used to analyze DNA ploidy with cell morphology at the single-cell level by using specialized image-processing software. Here we compared DNA ploidy obtained by FMI measured with that obtained flow cytometry (FCM). With FMI, we could evaluate the DNA ploidy in long-term preserved bone marrow smear samples after staining. We next analyzed the MK DNA ploidy in 42 bone marrow smear samples including 26 myeloid neoplasm cases, and we compared the DNA ploidy and platelet counts in the patients' peripheral blood; the production of platelets was significantly high compared to DNA ploidy in the myeloproliferative neoplasms group. The FMI method revealed that the patients with 5q- syndrome exhibited relatively low DNA ploidy despite high platelet counts, and this result suggested that increased DNA ploidy is not indispensable to abundant platelet production. The FMI method for DNA ploidy will be a useful tool to clarify the relationship between DNA ploidy and platelet production by MKs. |
| Keywords | fluorescence microscopy image analysis DNA ploidy megakaryocytes MDS with isolated del(5q) 5q- syndrome |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2018-06 |
| Volume | volume72 |
| Issue | issue3 |
| Publisher | Okayama University Medical School |
| Start Page | 249 |
| End Page | 256 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2018 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 29926002 |
