result 200 件
| FullText URL | fulltext.pdf |
|---|---|
| Author | Tanabe, Shihori| Quader, Sabina| Ono, Ryuichi| Tanaka, Hiroyoshi Y.| Cabral, Horacio| |
| Keywords | coronavirus EIF2 signaling microRNA molecular pathway network activation |
| Published Date | 2026-02-04 |
| Publication Title | International Journal of Molecular Sciences |
| Volume | volume27 |
| Issue | issue3 |
| Publisher | MDPI AG |
| Start Page | 1525 |
| ISSN | 1422-0067 |
| NCID | AA12038549 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2026 by the authors. |
| File Version | publisher |
| PubMed ID | 41683944 |
| DOI | 10.3390/ijms27031525 |
| CRID | 1871434918649812352 |
| Related Url | isVesionOf https://doi.org/10.3390/ijms27031525 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nurhamim, Mohammad| Yokoi, Aya| Hagiya, Hideharu| Maruyama, Takayuki| Ekuni, Daisuke| |
| Keywords | miR-324-5p saliva marker HIV periodontitis extracellular vesicles inflammation |
| Published Date | 2026-09-12 |
| Publication Title | International Journal of Molecular Sciences |
| Volume | volume27 |
| Issue | issue18 |
| Publisher | MDPI AG |
| Start Page | 8117 |
| ISSN | 1422-0067 |
| NCID | AA12038549 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2026 by the authors. |
| File Version | publisher |
| PubMed ID | 42794547 |
| DOI | 10.3390/ijms27188117 |
| Related Url | isVersionOf https://doi.org/10.3390/ijms27188117 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nakahara, Momoko| Kataoka, Kota| Maruyama, Takayuki| Nurhamim, Mohammad| Zhang, Yixuan| Fukuhara, Daiki| Uchida-Fukuhara, Yoko| Islam, Md Monirul| Morita, Manabu| Saito, Takashi| Ekuni, Daisuke| |
| Keywords | Alzheimer’s disease periodontitis diabetes mellitus microRNA mRNA |
| Published Date | 2026-08-23 |
| Publication Title | International Journal of Molecular Sciences |
| Volume | volume27 |
| Issue | issue17 |
| Publisher | MDPI AG |
| Start Page | 7542 |
| ISSN | 1422-0067 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2026 by the authors. |
| File Version | publisher |
| DOI | 10.3390/ijms27177542 |
| Related Url | isVersionOf https://doi.org/10.3390/ijms27177542 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Omura, Tomohiro| Nishiguchi, Hiroki| Kaneda, Haruka| Kitahiro, Yumi| Itohara, Kotaro| Yamamoto, Kazuhiro| Sakane, Toshiyasu| Yano, Ikuko| |
| Keywords | Parkinson’s disease microRNA Endoplasmic reticulum stress HRD1 SEL1L |
| Published Date | 2025-12-10 |
| Publication Title | BMC Research Notes |
| Volume | volume19 |
| Issue | issue1 |
| Publisher | Springer Science and Business Media LLC |
| Start Page | 19 |
| ISSN | 1756-0500 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2025. |
| File Version | publisher |
| PubMed ID | 41373032 |
| DOI | 10.1186/s13104-025-07604-6 |
| Web of Science KeyUT | 001661432100001 |
| Related Url | isVersionOf https://doi.org/10.1186/s13104-025-07604-6 |
| FullText URL | K0007464_abstract_review.pdf K0007464_fulltext.pdf K0007464_summary.pdf |
|---|---|
| Author | MOHAMMAD NURHAMIM| |
| Published Date | 2026-03-25 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第7464号 |
| Granted Date | 2026-03-25 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | English |
| Copyright Holders | © 2025 by the authors. |
| JaLCDOI | 10.18926/AMO/70450 |
|---|---|
| FullText URL | 80_2_075.pdf |
| Author | Lin, Deting| Horita, Masahiro| Watanabe, Masahito| Hasei, Joe| Ohtsuki, Takashi| Otsuka, Noriaki| Ichikawa, Chinatsu| Shimizu, Noriyuki| Naniwa, Shuichi| Ozaki, Toshifumi| Nishida, Keiichiro| |
| Abstract | A disintegrin and metalloproteinase 12 (ADAM12) is known to be involved in chondrocyte proliferation and is upregulated in the synovial tissue of osteoarthritis (OA). However, the underlying mechanisms of ADAM12 on rheumatoid arthritis (RA) synovial cell proliferation remain unknown. Here, we investigated the role of ADAM12 in the proliferation of RA synovial fibroblasts (RASFs). The expression and localization of ADAM12 in RA synovial tissues were examined by immunohistochemistry and compared with OA and healthy control (HC) synovial tissues. The effect of inflammatory cytokines (TNF-α, TGF-β1, and PDGF-BB) on ADAM12 expression in RASFs from RA patients was examined by real-time RT-PCR. The effect of ADAM12 knock-down by ADAM12 siRNA and ADAM12 overexpression on cell proliferation of RASFs were examined by WST-1 assay. ADAM12 was identified predominantly in RA synovial tissue rather than OA and HC synovial tissues. Stimulation with TGF-β1 upregulated the expression of ADAM12 and cell proliferation of RASFs. ADAM12 siRNA suppressed TGF-β1-induced cell proliferation of RASFs, while ADAM12 overexpression promoted the cell proliferation of RASFs. These findings demonstrate that ADAM12 may have a key role in TGF-β1-induced cell proliferation of synovial fibroblasts in patients with RA. |
| Keywords | rheumatoid arthritis synovial tissue TGF-β1 ADAM12 cell proliferation |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2026-04 |
| Volume | volume80 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 75 |
| End Page | 83 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2026 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 42025429 |
| Web of Science KeyUT | 001761342100001 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Gao, Tong| Ito, Sachio| Moh-Moh-Aung, Aye| Wang, Tianyi| Fujisawa, Masayoshi| Ohara, Toshiaki| Yoshimura, Teizo| Matsukawa, Akihiro| |
| Keywords | SPRED2 p53 KLF4 miR-506-3p stemness |
| Published Date | 2026-01-20 |
| Publication Title | Cancer Biology & Medicine |
| Publisher | China Anti-cancer Association |
| ISSN | 2095-3941 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | ©2026 The Authors. |
| File Version | publisher |
| PubMed ID | 41560328 |
| DOI | 10.20892/j.issn.2095-3941.2025.0247 |
| Related Url | isVersionOf https://doi.org/10.20892/j.issn.2095-3941.2025.0247 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nurhamim, Mohammad| Zhang, Yixuan| Nakahara, Momoko| Fukuhara, Daiki| Nagashima, Yosei| Maruyama, Takayuki| Morita, Manabu| Ekuni, Daisuke| |
| Keywords | extracellular vesicles miR-128-3p mRNA inflammation periodontitis renal inflammation lipopolysaccharide |
| Published Date | 2025-12-03 |
| Publication Title | Dentistry Journal |
| Volume | volume13 |
| Issue | issue12 |
| Publisher | MDPI AG |
| Start Page | 577 |
| ISSN | 2304-6767 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2025 by the authors. |
| File Version | publisher |
| DOI | 10.3390/dj13120577 |
| Related Url | isVersionOf https://doi.org/10.3390/dj13120577 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Watanabe, Mayu| Eguchi, Jun| Kurooka, Naoko| Eto, Eriko| Masuyama, Hisashi| Wada, Jun| |
| Keywords | glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) gestational diabetes mellitus (GDM) perinatal outcomes placenta triglyceride (TG) |
| Published Date | 2025-10-10 |
| Publication Title | Frontiers in Clinical Diabetes and Healthcare |
| Volume | volume6 |
| Publisher | Frontiers Media SA |
| Start Page | 1682012 |
| ISSN | 2673-6616 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2025 Watanabe, Eguchi, Kurooka, Eto, Masuyama and Wada. |
| File Version | publisher |
| PubMed ID | 41142641 |
| DOI | 10.3389/fcdhc.2025.1682012 |
| Web of Science KeyUT | 001599396300001 |
| Related Url | isVersionOf https://doi.org/10.3389/fcdhc.2025.1682012 |
| FullText URL | fulltext.pdf suppl.pdf |
|---|---|
| Author | Kawagoe, Soichiro| Matsusaki, Motonori| Mabuchi, Takuya| Ogasawara, Yuto| Watanabe, Kazunori| Ishimori, Koichiro| Saio, Tomohide| |
| Keywords | heat shock factor 1 oxidative hyper-oligomerization biological phase transition stress response biophysics |
| Published Date | 2025-01-30 |
| Publication Title | JACS Au |
| Volume | volume5 |
| Issue | issue2 |
| Publisher | American Chemical Society (ACS) |
| Start Page | 606 |
| End Page | 617 |
| ISSN | 2691-3704 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2025 The Authors. |
| File Version | publisher |
| PubMed ID | 40017748 |
| DOI | 10.1021/jacsau.4c00578 |
| Web of Science KeyUT | 001411241900001 |
| Related Url | isVersionOf https://doi.org/10.1021/jacsau.4c00578 |
| JaLCDOI | 10.18926/AMO/68646 |
|---|---|
| FullText URL | 79_2_081.pdf |
| Author | Kawada, Tatsushi| Kobayashi, Yasuyuki| Tsugawa, Takuji| Tsuboi, Kazuma| Katayama, Satoshi| Iwata, Takehiro| Bekku, Kensuke| Kobayashi, Tomoko| Edamura, Kohei| Ebara, Shin| Araki, Motoo| |
| Abstract | We retrospectively evaluated the oncologic outcomes of paclitaxel, cisplatin, and gemcitabine (PCG) with those of gemcitabine and cisplatin (GC) as neoadjuvant chemotherapy in muscle-invasive bladder cancer (MIBC) patients. The primary outcome was efficacy: pathological complete response (pCR), ypT0N0; and pathological objective response (pOR), ypT0N0, ≤ ypT1N0, or ypT0N1. Secondary outcomes included overall survival (OS), recurrence-free survival (RFS), predictive factors for pOR, OS, and RFS, and hematologic adverse events (AEs). Among 113 patients treated (PCG, n=28; GC, n=85), similar pOR and pCR rates were achieved by the groups (pOR: PCG, 57.1% vs. GC, 49. 4%; p=0.52; pCR: PCG, 39.3% vs. GC, 29.4%; p=0.36). No significant differences were observed in OS (p=1.0) or RFS (p=0.20). Multivariate logistic regression analysis showed that hydronephrosis (odds ratio [OR] 0.32, 95%CI: 0.11-0.92) and clinical node-positive status (cN+) (OR 0.22, 95%CI: 0.050-0.99) were significantly associated with a decreased probability of pOR. On multivariate Cox regression analyses, pOR achievement was associated with improved OS (hazard ratio [HR] 0.23, 95%CI: 0.10-0.56) and RFS (HR 0.30, 95%CI: 0.13-0.67). There were no significant between-group differences in the incidence of grade ≥ 3 hematologic AEs or dose-reduction required, but the PCG group had a higher incidence of grade 4 neutropenia. |
| Keywords | urothelial carcinoma paclitaxel cisplatin gemcitabine neoadjuvant |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2025-04 |
| Volume | volume79 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 81 |
| End Page | 92 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2025 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 40302279 |
| Web of Science KeyUT | 001485544200003 |
| JaLCDOI | 10.18926/AMO/67868 |
|---|---|
| FullText URL | 78_6_429.pdf |
| Author | Kubota, Risa| Bekku, Kensuke| Katayama, Satoshi| Iwata, Takehiro| Nishimura, Shingo| Edamura, Kohei| Kobayashi, Tomoko| Kobayashi, Yasuyuki| Araki, Motoo| |
| Abstract | Although partial nephrectomy (PN) is preferred over radical nephrectomy (RN) for preserving renal function in patients with cT1 renal cancer, its impact on cardiovascular events (CVe) remains controversial. This study aimed to compare PN and RN in regard to the occurrence of CVe, including cerebrovascular events and exacerbation of hypertension (HT). We retrospectively analyzed 418 consecutive patients who underwent PN or RN for cT1 renal cancer. Propensity score-matching analysis was used to adjust for imbalances between patients who underwent PN and RN, leaving 102 patients in each group. The 5-year probability of cumulative CVe incidence was 6% in the PN group and 12% in the RN group (p=0.03), with a median follow-up of 73.5 months. The statistical significance was retained after propensity score matching for patients without preoperative proteinuria (p=0.03). For all CVe including cerebrovascular events and exacerbation of HT analyzed, PN provided a lower probability of occurrence than RN in patients with small renal cancers. |
| Keywords | chronic kidney disease hypertension nephrectomy proteinuria |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2024-12 |
| Volume | volume78 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 429 |
| End Page | 437 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2024 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 39719315 |
| Web of Science KeyUT | 001397799300001 |
| JaLCDOI | 10.18926/AMO/67549 |
|---|---|
| FullText URL | 78_4_331.pdf |
| Author | Furukawa, Chieko| Tachibana, Tomoyasu| Nobuhisa, Tetsuji| Kanie, Yuichiro| Wani, Yoji| Matsumoto, Jun-Ya| Kariya, Akifumi| Sato, Asuka| Ishikawa, Iichiro| Naoi, Yuto| Ando, Mizuo| |
| Abstract | No previous study has published magnetic resonance imaging (MRI) findings for a subglottic pleomorphic adenoma. Here, we describe the case of a 62-year-old man with a subglottic pleomorphic adenoma. Endoscopic findings revealed a smooth-surfaced tumor arising from the subglottic posterior wall. MRI revealed the lesion as an isointense region on T1-weighted images, which was homogeneously enhanced. This lesion showed a heterogeneously hyperintense region on T2-weighted images. Diffusion-weighted imaging (DWI) showed slightly high intensity in the same area, with a normal or only slightly high apparent diffusion coefficient (ADC). Laryngomicrosurgery was performed for transoral excision of the subglottic tumor, resulting in a postsurgical diagnosis of pleomorphic adenoma. |
| Keywords | subglottis pleomorphic adenoma MRI transoral surgery |
| Amo Type | Case Report |
| Publication Title | Acta Medica Okayama |
| Published Date | 2024-08 |
| Volume | volume78 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 331 |
| End Page | 335 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2024 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 39198987 |
| Web of Science KeyUT | 001310557400001 |
| JaLCDOI | 10.18926/AMO/66664 |
|---|---|
| FullText URL | 78_1_001.pdf |
| Author | Sun, Cuiming| Matsukawa, Akihiro| |
| Abstract | Liver fibrosis, which ultimately leads to liver cirrhosis and hepatocellular carcinoma, is a major health burden worldwide. The progression of liver fibrosis is the result of the wound-healing response of liver to repeated injury. Hepatic macrophages are cells with high heterogeneity and plasticity and include tissue-resident macrophages termed Kupffer cells, and recruited macrophages derived from circulating monocytes, spleen and peritoneal cavity. Studies have shown that hepatic macrophages play roles in the initiation and progression of liver fibrosis by releasing inflammatory cytokines/chemokines and pro-fibrogenic factors. Furthermore, the development of liver fibrosis has been shown to be reversible. Hepatic macrophages have been shown to alternately regulate both the regression and turnover of liver fibrosis by changing their phenotypes during the dynamic progression of liver fibrosis. In this review, we summarize the role of hepatic macrophages in the progression and regression of liver fibrosis. |
| Keywords | ERK-MAPK SPRED2 fibrosis macrophages |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2024-02 |
| Volume | volume78 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 8 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2024 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 38419308 |
| Web of Science KeyUT | 001203658200006 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Obata, Taisuke| Tsutsumi, Koichiro| Ueta, Eijiro| Oda, Takashi| Kikuchi, Tatsuya| Ako, Soichiro| Fujii, Yuki| Yamazaki, Tatsuhiro| Uchida, Daisuke| Matsumoto, Kazuyuki| Horiguchi, Shigeru| Kato, Hironari| Okada, Hiroyuki| Otsuka, Motoyuki| |
| Published Date | 2023-12-12 |
| Publication Title | Molecular Therapy Nucleic Acids |
| Volume | volume34 |
| Publisher | Cell Press |
| Start Page | 102054 |
| ISSN | 2162-2531 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 The Author(s). |
| File Version | publisher |
| PubMed ID | 38111913 |
| DOI | 10.1016/j.omtn.2023.102054 |
| Web of Science KeyUT | 001146413100001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.omtn.2023.102054 |
| JaLCDOI | 10.18926/AMO/65751 |
|---|---|
| FullText URL | 77_4_407.pdf |
| Author | Hotta, Kensuke| Kobayashi, Takaomi| |
| Abstract | This study aimed to investigate the usefulness of force-controlled pelvic stress radiographs in the evaluation and treatment of fragility fractures of the pelvis (FFP) using a functional treatment strategy. We conducted a retrospective study of 55 geriatric patients with FFP who underwent pelvic stress radiographs on admission. The differences in the sacral width, pelvic ring width, and medial femoral head width between the radiographs with and without the Sam Sling II M size were defined as Δ sacral width, Δ pelvic ring width, and Δ medial femoral head width, respectively. We used Pearson’s correlation test to assess the relationship between the degree of radiographic instability and the Johns Hopkins highest level of mobility scale (JH-HLM) at 10-days postadmission. Conventional receiver-operating-characteristic curve analysis was used to identify cases requiring surgery using the best cutoff value for radiographic instability. The JH-HLM was significantly correlated with Δ sacral width (r=−0.401, p=0.017), but not with Δ pelvic ring width (r=−0.298, p=0.080) nor with Δ medial femoral head width (r= −0.261, p=0.128). The best cutoff value of Δ sacral width in identifying surgical cases was 10.7 mm (sensitivity 75.0%, specificity 98.0%). Force-controlled pelvic stress radiographs could be helpful in assessing the need for surgery on admission. |
| Keywords | fragility fracture of the pelvis functional treatment strategy Sam Sling stress radiograph Johns Hopkins highest level of mobility scale |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2023-08 |
| Volume | volume77 |
| Issue | issue4 |
| Publisher | Okayama University Medical School |
| Start Page | 407 |
| End Page | 414 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2023 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 37635141 |
| Web of Science KeyUT | 001163659800001 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Shiotani, Toshio| Sugimoto, Seiichiro| Tomioka, Yasuaki| Yamamoto, Haruchika| Tanaka, Shin| Miyoshi, Kentaroh| Suzawa, Ken| Shien, Kazuhiko| Yamamoto, Hiromasa| Okazaki, Mikio| Toyooka, Shinichi| |
| Keywords | Biomarker Chronic lung allograft dysfunction Lung transplantation Living -donor lobar lung transplantation Micro-RNA |
| Published Date | 2023-04 |
| Publication Title | Heliyon |
| Volume | volume9 |
| Issue | issue4 |
| Publisher | Elsevier |
| Start Page | e14903 |
| ISSN | 2405-8440 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 The Authors. |
| File Version | publisher |
| PubMed ID | 37025782 |
| DOI | 10.1016/j.heliyon.2023.e14903 |
| Web of Science KeyUT | 000998641100001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.heliyon.2023.e14903 |
| JaLCDOI | 10.18926/AMO/64123 |
|---|---|
| FullText URL | 76_6_723.pdf |
| Author | Zhang, Quan| Yang, Lixia| Wan, Guozhen| Zhang, Xiaoqiang| Wang, Ying| Zhao, Guannan| |
| Abstract | The diagnostic value of microRNA-377 (miR-377) in patients with acute coronary syndrome (ACS) and explored miR-377’s potential mechanisms. We performed an qRT-PCR to assess serum miR-377 levels in ACS patients and coronary artery ligation rat models. The diagnostic value of miR-377 was evaluated by determining the ROC curve. An ELISA assay was conducted to detect the model rat endothelial damage markers von Willebrand factor (vWF) and heart-type fatty acid binding protein (H-FABP), and proinflammatory cytokines TNF-α, IL-6, and IL-1β. The serum miR-377 level was elevated in the ACS patients and significantly increased in the ACS rats. MiR-377 has a high diagnostic value in ACS patients, with a 0.844 ROC, 76.47% specificity, and 87.10% sensitivity. MiR-377 was positively correlated with the expressions of vWF, H-FABP, cTnI, TNF-α, IL-6, and IL-1β. In ACS rats, reducing the expression of miR-377 significantly inhibited the increases in vWF, H-FABP, TNF-α, IL-6, and IL-1β. An elevated miR-377 level can be used as a diagnostic marker in patients with ACS. A reduction of miR-377 may alleviate ACS by improving myocardial damage such as endothelial injury and the inflammatory response. |
| Keywords | microRNA-377 acute coronary syndrome diagnosis endothelial injury inflammatory |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-12 |
| Volume | volume76 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 723 |
| End Page | 730 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 36549775 |
| Web of Science KeyUT | 000905195100013 |
| JaLCDOI | 10.18926/AMO/64113 |
|---|---|
| FullText URL | 76_6_635.pdf |
| Author | Zhang, Cuicui| Ji, Yanan| Wang, Qin| Ruan, Lianying| |
| Abstract | To investigate the association between serum miR-338-3p levels and neonatal acute respiratory distress syndrome (ARDS) and its mechanism. The relative miR-338-3p expression in serum was detected by quantitative real-time RT-PCR. Interleukin-1beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α) levels were detected by ELISAs. A receiver operating characteristic (ROC) curve analysis of serum miR-338-3p evaluated the diagnosis of miR-338-3p in neonatal ARDS. Pearson’s correlation analysis evaluated the correlation between serum miR-338-3p and neonatal ARDS clinical factors. Flow cytometry evaluated apoptosis, and a CCK-8 assay assessed cell viability. A luciferase assay evaluated the miR-338-3p/AKT3 relationship. The miR- 338-3p expression was decreased in neonatal ARDS patients and in lipopolysaccharide (LPS)-treated cells. The ROC curve showed the accuracy of miR-338-3p for evaluating neonatal ARDS patients. The correlation analysis demonstrated that miR-338-3p was related to PRISM-III, PaO2/FiO2, oxygenation index, IL-1β, IL-6, and TNF-α in neonatal ARDS patients. MiR-338-3p overexpression inhibited the secretion of inflammatory components, stifled cell apoptosis, and LPS-induced advanced cell viability. The double-luciferase reporter gene experiment confirmed that miR-338-3p negatively regulates AKT3 mRNA expression. Serum miR-338-3p levels were related to the diagnosis and severity of neonatal ARDS, which may be attributed to its regulatory effect on inflammatory response in ARDS. |
| Keywords | miR-338-3p AKT3 neonatal ARDS inflammation diagnosis |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-12 |
| Volume | volume76 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 635 |
| End Page | 643 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 36549765 |
| Web of Science KeyUT | 000905195100003 |
| JaLCDOI | 10.18926/AMO/64112 |
|---|---|
| FullText URL | 76_6_625.pdf |
| Author | Liu Zhisheng| Li, Qingmei| Wang, Ye| Ge, Yunjie| |
| Abstract | This investigation aimed to uncover the impact of a long noncoding RNA, SET-binding factor 2 antisense RNA1 (SBF2-AS1) on the malignant progression of gastric cancer (GC) and to further explore its underlying mechanism. SBF2-AS1 expression was quantified by qRT-PCR in GC cell lines and GC tissues. In vitro loss-of-function studies of SBF2-AS1, accompanied by flow cytometry, CCK-8, and cell invasion tests, were applied to elucidate the impact of SBF2-AS1 on the tumor progression of GC cells. Finally, Western blotting and a luciferase assay were used to detect WNT/LRP5 signaling pathway activation. SBF2-AS1 was aberrantly expressed in GC cell lines (p<0.05) and GC tissues (p<0.05). Cell invasive and proliferative capabilities were inhibited via SBF2-AS1 knockdown, resulting in apoptosis of NCI-N87 and MKN74 cells. Additionally, online database analysis uncovered a positive correlation between SBF2-AS1 and the Wnt/LRP5 signaling pathway (p<0.05). SBF2-AS1 knockdown blocked the Wnt/LRP5 signaling pathway, whereas the effects of SBF2-AS1 knockdown on the malignant genotype of MKN74 as well as NCI-N87 cells were partially restored by triggering the Wnt/ LRP5 signaling pathway. High expression of SBF2-AS1 was found in GC, the malignant progression of which was repressed via SBF2-AS1 knockdown by inhibiting the Wnt/LRP5 signaling pathway. |
| Keywords | gastric cancer (GC) SET-binding factor 2 antisense RNA1 (SBF2-AS1) invasion proliferation signaling |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2022-12 |
| Volume | volume76 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 625 |
| End Page | 633 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | Copyright Ⓒ 2022 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 36549764 |
| Web of Science KeyUT | 000905195100002 |
