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FullText URL JRNC_313_1_169.pdf
Author Kudo, Ken-ichi| Hanafusa, Tadashi| Ono, Toshiro|
Keywords Monoterpenes Linalool X-ray irradiation Reactive oxygen species SSB
Note The final publication is available at Springer|
Published Date 2017-07
Publication Title Journal of Radioanalytical and Nuclear Chemistry
Volume volume313
Issue issue1
Publisher Springer
Start Page 169
End Page 174
ISSN 0236-5731
NCID AA11120189
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
DOI 10.1007/s10967-017-5268-0
Related Url https://doi.org/10.1007/s10967-017-5268-0
FullText URL Chem_Mater_29_5_2150.pdf
Author Morimoto, Naoki| Suzuki, Hideyuki| Takeuchi, Yasuo| Kawaguchi, Shogo| Kunisu, Masahiro| Bielawski, Christopher W.| Nishina, Yuta|
Published Date 2017-03-02
Publication Title Chemistry of Materials
Volume volume29
Issue issue5
Publisher American Chemical Society
Start Page 2150
End Page 2156
ISSN 15205002
NCID AA12096771
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders Copyright © 2017 American Chemical Society
File Version author
DOI 10.1021/acs.chemmater.6b04807
Web of Science KeyUT 000396639400027
Related Url isVersionOf https://doi.org/10.1021/acs.chemmater.6b04807
JaLCDOI 10.18926/AMO/55210
FullText URL 71_3_259.pdf
Author Kawana, Shinichi| Yamamoto, Hiromasa| Maki, Yuho| Sugimoto, Seiichiro| Toyooka, Shinichi| Miyoshi, Shinichiro|
Abstract  Primary sternal chondrosarcoma is a rare malignant tumor that is refractory to chemotherapy and radiation. Effective therapy is radical resection of the tumor. We present two patients with primary sternal chondrosarcoma who underwent a radical resection of the lower half of the sternum and bilateral ribs, followed by reconstruction with 2 sheets of polypropylene mesh layered orthogonally. The patients have maintained almost the same pulmonary function as preoperative values, with stability of the chest wall. Although there are various ways to reconstruct the anterior chest wall, reconstruction with polypropylene mesh layered orthogonally is an easy-to-use and sufficient method.
Keywords chondrosarcoma sternum reconstruction polypropylene mesh
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 259
End Page 262
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655947
JaLCDOI 10.18926/AMO/55208
FullText URL 71_3_249.pdf
Author Washio, Kana| Muraoka, Michiko| Kanamitsu, Kiichiro| Oda, Megumi| Shimada, Akira|
Abstract  We diagnosed a female infant with Langerhans cell histiocytosis (LCH) who was refractory to conventional chemotherapy. She showed refractory inflammation that was complicated with hemophagocytic lymphohistiocytosis (HLH) during LCH chemotherapy; therefore, we changed the protocol to HLH2004 (dexamethasone, cyclosporine A and VP16). However, there were no signs of hematological recovery. We therefore performed cord blood transplantation with reduced-intensity conditioning, and she achieved complete remission for over 2 years. As salvage therapy for refractory LCH, hematopoietic stem cell transplantation may be a good therapeutic choice, especially when LCH is complicated with HLH.
Keywords langerhans cell histiocytosis (LCH) hemophagocytic lymphohistiocytosis (HLH) hematopoietic stem cell transplantation (HSCT) reduced-intensity conditioning (RIC) refractory
Amo Type Case Report
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 249
End Page 254
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655945
JaLCDOI 10.18926/AMO/55207
FullText URL 71_3_241.pdf
Author Iwamuro, Masaya| Tanaka, Shouichi| Moritou, Yuki| Inaba, Tomoki| Higashi, Reiji| Kusumoto, Chiaki| Yunoki, Naoko| Ishikawa, Shin| Okamoto, Yuko| Kawai, Yoshinari| Kitada, Ken-ichi| Takenaka, Ryuta| Toyokawa, Tatsuya| Okada, Hiroyuki|
Abstract  Most gastric bezoars can be treated with endoscopic fragmentation combined with or without cola dissolution, whereas laparotomy or laparoscopic surgery is generally inevitable for small intestinal bezoars because they cause small bowel obstruction. Therefore, early diagnosis and management of gastric bezoars are necessary to prevent bezoar-induced ileus. To investigate the incidence of overlooked gastric bezoars during the initial esophagogastroduodenoscopy, we retrospectively reviewed the cases of 27 patients diagnosed with gastrointestinal bezoars. The bezoars were diagnosed using esophagogastroduodenoscopy (n=25), abdominal ultrasonography (n=1), and barium follow-through examination (n=1). Bezoars were overlooked in 9/25 patients (36.0%) during the initial endoscopy examination because the bezoars were covered with debris in the stomach. Of the 9 patients, 8 had concomitant gastric ulcers, and the other patient had gastric lymphoma. Although a computed tomography (CT) scan was performed before the second-look endoscopy in 8 of the 9 patients, the bezoars were mistaken as food debris on CT findings and were overlooked in these patients. In conclusion, gastric bezoars may not be discovered during the initial esophagogastroduodenoscopy and CT scan. In cases with debris in the stomach, second-look endoscopy is essential to detect bezoars.
Keywords bezoar gastric ulcer foreign bodies phytobezoar
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 241
End Page 247
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655944
JaLCDOI 10.18926/AMO/55206
FullText URL 71_3_233.pdf
Author Shimoyama, Kyoko| Osako, Tomo| Mitsuhashi, Toshiharu| Akiyama, Futoshi| Iwase, Takuji|
Abstract Ductal carcinoma in situ (DCIS) of the breast has no potential to metastasize, but over 20% of cases preoperatively diagnosed as DCIS are upstaged on final pathology. The rates of upstaging and the predictors for invasion on final pathology were evaluated. For 240 primary breast cancers, radiological findings on mammography, ultrasonography, and magnetic resonance imaging were investigated along with pathological and clinical information. Univariate and multivariate analyses were performed to identify predictors of potential invasion. Of the 240 breast cancers, 68 (28.3%) showed invasion on final pathology, and 5 (2.5%) had sentinel node metastasis. The multivariate analysis identified five independent predictors: non-mass lesions >2.4 cm on ultrasonography (odds ratio [OR] 2.84, 95% confidence interval [CI] 1.02-7.95, p=0.047), comedo-type histology (OR 6.89, 95% CI 1.89-25.08, p<0.01), solid-type histology (OR 7.97, 95% CI 2.08-30.49, p<0.01), palpable mass (OR 2.63, 95% CI 1.05-6.64, p=0.04), and bloody nipple discharge (OR 4.61, 95% CI 1.20-17.66, p=0.02). These five predictors were associated with invasion on final pathology and may help select candidates for sentinel node biopsy.
Keywords breast cancer DCIS breast needle biopsy imaging examination sentinel node biopsy
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 233
End Page 240
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655943
JaLCDOI 10.18926/AMO/55205
FullText URL 71_3_227.pdf
Author Sasaki, Katsumi| Wada, Koichiro| Araki, Motoo| Kobayashi, Yasuyuki| Sugimoto, Morito| Ebara, Shin| Watanabe, Toyohiko| Nasu, Yasutomo|
Abstract  We elucidate the fate of cases clinically suspected of carcinoma in situ (Cis) of the upper tract with serial ureteroscopy. Of 143 patients who underwent ureteroscopy for suspected upper tract urothelial carcinoma (UTUC) between January 2008 and February 2016, 12 cases with consistently positive urine cytology and poorly detectable upper-tract malignancies by imaging were reviewed. In these 12 patients, 19 ureteroscopy procedures (25 renal units) were performed. Vesical random biopsy was performed before the 1st ureteroscopy to exclude malignancy of the bladder in all 12 cases. Median follow-up was 42 (13-67) months. Positive biopsy results at the 1st ureteroscopy were obtained in 3 (25%) patients and all were diagnosed wth Cis of the upper tract. Two (17%) of 9 patients who were negative or inconclusive at the 1st ureteroscopy were finally diagnosed as UTUC, but plural ureteroscopy procedures were needed for the diagnoses in both. Carcinoma of the bladder appeared in 5 (42%) patients during follow-up, despite the earlier ruling out of vesical malignancy. Four (33%) of those 5 patients never developed upper-tract urothelial carcinoma during follow-up. Caution is required before undertaking radical surgery for cases clinically suspected of Cis of the upper tract. In our experience, only 42% of such patients developed UTUC; another 33% eventually developed carcinoma of the bladder without UTUC.
Keywords carcinoma in situ carcinoma of the bladder upper tract urothelial carcinoma ureteroscopy urine cytology
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 227
End Page 232
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655942
JaLCDOI 10.18926/AMO/55204
FullText URL 71_3_219.pdf
Author Takakura, Hiroaki| Domae, Shohei| Ono, Toshiro| Sasaki, Akira|
Abstract  Anticancer drugs induce cell-cycle arrest and apoptosis not only in tumor cells, but also in immune cells. However, many preclinical and clinical findings show that some chemotherapeutic agents can improve the antitumor efficacy of immunotherapy. We immunohistochemically analyzed the degree of immune cell infiltration and the relevance of programmed cell death 1 ligand-1 (PD-L1) expression in surgically resected oral squamous cell carcinoma (OSCC) specimens from patients who had undergone pretreatment with certain chemotherapies and other patients without pretreatment. We divided the patients into the group of neoadjuvant chemotherapy (NAC) patients (n=8) and the nNAC (without NAC) patient group (n=10). We observed that NAC induced infiltrations of CD4, CD8 T cells and CD56 NK cells into the tumor microenvironment. Decreased numbers of Tregs and PD-1-positive cells were observed in the NAC group. No significant difference was observed in the degree of immune-cell infiltration between the patient groups except for CD56 NK cells in the stroma and PD-1 cells in cancer nests. Eighty percent of the nNAC specimens showed intermediate-to-strong PD-L1 protein expression, whereas 75% of the NAC specimens showed down-regulation of the PD-L1 protein, indicating the effectiveness of the chemotherapeutic treatment before surgery.
Keywords oral squamous cell carcinoma programmed cell death 1 ligand-1 tumor microenvironment neoadjuvant chemotherapy immunohistochemistry
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 219
End Page 226
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655941
JaLCDOI 10.18926/AMO/55203
FullText URL 71_3_209.pdf
Author Xin, Zhao| Tsuda, Toshihide| Doi, Hiroyuki|
Abstract We evaluated how exposure to airborne volatile organic compounds emitted from a plastic recycling facility affected nearby residents, in a cross-sectional study. Individuals>10 years old were randomly sampled from 50 households at five sites and given questionnaires to complete. We categorized the subjects by distance from the recycling facility and used this as a proxy measure for pollutant exposure. We sought to improve on a preceding study by generating new findings, improving methods for questionnaire distribution and collection, and refining site selection. We calculated the odds of residents living 500 or 900 m away from the facility reporting mucocutaneous and respiratory symptoms using a reference group of residents 2,800 m away. Self-reported nasal congestion (odds ratio=3.0, 95% confidence interval=1.02-8.8), eczema (5.1, 1.1-22.9), and sore throat (3.9, 1.1-14.1) were significantly higher among residents 500 m from the facility. Those 900 m away were also considerably more likely to report experiencing eczema (4.6, 1.4-14.9). Air pollution was found responsible for significantly increased reports of mucocutaneous and respiratory symptoms among nearby residents. Our findings confirm the effects of pollutants emitted from recycling facilities on residents’ health and clarify that study design differences did not affect the results.
Keywords air pollutants volatile organic compounds recycling facility mucocutaneous symptoms respiratory symptoms
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 209
End Page 217
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655940
JaLCDOI 10.18926/AMO/55202
FullText URL 71_3_201.pdf
Author Matsuda, Miwa| Sasaki, Aiko| Shimizu, Keiko| Kamada, Yasuhiko| Noguchi, Soichi| Hiramatsu, Yuji| Nakatsuka, Mikiya|
Abstract  Vascular dysfunction has been reported in women with recurrent pregnancy loss (RPL). We investigated the severity of vascular dysfunction in non-pregnant women with RPL and its correlation with anti-heat shock protein (HSP) antibodies that are known to induce arteriosclerosis. We measured the serum anti-HSP60 antibodies, anti-HSP70 antibodies, and anti-phospholipid antibodies (APA) in 68 women with RPL and 29 healthy controls. Among the women with RPL, 14 had a diagnosis of antiphospholipid syndrome (APS), and in the remaining 54, the causes for RPL were unexplained. Compared to the controls, the brachial-ankle pulse wave velocity (baPWV), carotid augmentation index (cAI), and uterine artery pulsatility index (PI) were all significantly higher in the women with both APS and unexplained RPL. Compared to the controls, the anti-HSP60 antibody levels were significantly higher in the APA-positive group of women with unexplained RPL, and the anti-HSP70 antibody levels were significantly higher in APS and APA-positive group of women with unexplained RPL. However, the anti-HSP60 and anti-HSP70 antibody levels did not correlate with the values of baPWV or cAI. Our results demonstrated anti-HSP60 and anti-HSP70 antibodies are increased in women with unexplained RPL. Further studies are needed to elucidate the roles of anti-HSP antibodies and their pathophysiology in unexplained RPL.
Keywords recurrent pregnancy loss autoantibody heat shock protein pulse wave velocity arterial stiffness
Amo Type Original Article
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 201
End Page 208
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655939
JaLCDOI 10.18926/AMO/55201
FullText URL 71_3_191.pdf
Author Kobayashi, Katsuhiro| Akiyama, Tomoyuki| Agari, Takashi| Sasaki, Tatsuya| Shibata, Takashi| Hanaoka, Yoshiyuki| Akiyama, Mari| Endoh, Fumika| Oka, Makio| Date, Isao|
Abstract  Electroencephalogram (EEG) data include broadband electrical brain activity ranging from infra-slow bands (< 0.1 Hz) to traditional frequency bands (e.g., the approx. 10 Hz alpha rhythm) to high-frequency bands of up to 500 Hz. High-frequency oscillations (HFOs) including ripple and fast ripple oscillations (80-200 Hz and>200 / 250 Hz, respectively) are particularly of note due to their very close relationship to epileptogenicity, with the possibility that they could function as a surrogate biomarker of epileptogenicity. In contrast, physiological high-frequency activity plays an important role in higher brain functions, and the differentiation between pathological / epileptic and physiological HFOs is a critical issue, especially in epilepsy surgery. HFOs were initially recorded with intracranial electrodes in patients with intractable epilepsy as part of a long-term invasive seizure monitoring study. However, fast oscillations (FOs) in the ripple and gamma bands (40-80 Hz) are now noninvasively detected by scalp EEG and magnetoencephalography, and thus the scope of studies on HFOs /FOs is rapidly expanding.
Keywords fast oscillations, epilepsy electroencephalogram time-frequency analysis
Amo Type Review
Publication Title Acta Medica Okayama
Published Date 2017-06
Volume volume71
Issue issue3
Publisher Okayama University Medical School
Start Page 191
End Page 200
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language English
Copyright Holders CopyrightⒸ 2017 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 28655938
FullText URL K0005453_other1.pdf
Author Nobumoto, Etsuko| Masuyama, Hisashi| Hiramatsu, Yuji| Sugiyama, Takashi| Kusaka, Hideto| Toyoda, Nagayasu|
Keywords New GDM criteria Perinatal complications Obesity 75-g oral glucose tolerance test
Note The final publication is available at Springer| 学位審査副論文|
Published Date 2015-09
Publication Title Diabetology International
Volume volume6
Issue issue3
Publisher Springer Japan
Start Page 226
End Page 231
ISSN 21901678
NCID AA12503387
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
File Version author
DOI 10.1007/s13340-014-0193-8
Web of Science KeyUT 000366631000009
Related Url http://doi.org/10.1007/s13340-014-0193-8 http://ousar.lib.okayama-u.ac.jp/55005
FullText URL J_Comp_Neurol_525_7_1586.pdf
Author Tamura, Kei| Kobayashi, Yasuhisa| Hirooka, Asuka| Takanami, Keiko| Oti, Takumi| Jogahara, Takamichi| Oda, Sen-ichi| Sakamoto, Tatsuya| Sakamoto, Hirotaka|
Keywords RRID AB_2060157 RRID: AB_2571636 RRID: AB_626757 Suncus murinus (suncus) gastrin-releasing peptide male reproductive function sexual dimorphism spinal cord
Note This is the peer reviewed version of the following article: Tamura K. Kobayashi Y. Hirooka A. et al. Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus). J Comp Neurol. 2017;525:1601–1613. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.| 2018年5月公開予定|
Published Date 2017-05-01
Publication Title Journal of Comparative Neurology
Volume volume525
Issue issue7
Publisher Wistar Institute of Anatomy and Biology
Start Page 1586
End Page 1598
ISSN 0021-9967
NCID AA00695917
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 27804131
DOI 10.1002/cne.24138
Web of Science KeyUT 000397559700004
Related Url https://doi.org/10.1002/cne.24138
FullText URL JoAmChemSoc_2017_139_12_4376.pdf
Author Niho, Akiko| Yoshizawa, Susumu| Tsukamoto, Takashi| Kurihara, Marie| Tahara, Shinya| Nakajima, Yu| Mizuno, Misao| Kuramochi, Hikaru| Tahara, Tahei| Mizutani, Yasuhisa| Sudo, Yuki|
Note This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher.|
Published Date 2017-03-29
Publication Title Journal of the American Chemical Society
Volume volume139
Issue issue12
Publisher American Chemical Society
Start Page 4376
End Page 4389
ISSN 00027863
NCID AA00692602
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
File Version author
PubMed ID 28257611
DOI 10.1021/jacs.6b12139
Web of Science KeyUT 000398247100034
Related Url isVersionOf https://doi.org/10.1021/jacs.6b12139
FullText URL Acta_Biomater_2017_5.pdf
Author Okada, Masahiro| Nakai, Akira| Hara Emilio, Satoshi| Taguchi, Tetsushi| Nakano, Takayoshi| Matsumoto, Takuya|
Keywords Hydroxyapatite Nanoparticle Solid adhesive Wet adhesion
Published Date 2017-05
Publication Title Acta Biomaterialia
Volume volume57
Publisher Elsevier B.V.
Start Page 404
End Page 413
ISSN 1742-7061
NCID AA12033647
Content Type Journal Article
language English
OAI-PMH Set 岡山大学
Copyright Holders © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
File Version author
PubMed ID 28483692
DOI 10.1016/j.actbio.2017.05.014
Web of Science KeyUT 000405041900035
Related Url https://doi.org/10.1016/j.actbio.2017.05.014
Title Alternative Introduction to the activities of the Regional Associations for Laboratory Animal Science
FullText URL poalas_033_040_047.pdf
Author Okayama Association for Laboratory Animal Science|
Abstract わが国には現在11 の地区研究会があり、独自の活動が展開されています。本報では、各地区研究会の間での情報交換、交流などを意図して、平成28 年度の活動について北から順に紹介します。 なお、会長、事務局担当者及び講演者、話題提供者の方々のお名前は敬称を省略させていただきました。詳細な内容をご希望の方は各地区研究会の事務局にお問い合わせ下さい。
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 40
End Page 47
language Japanese
File Version publisher
Title Alternative Introduction of Laboratory Animal Facilities in the Life Science Research Center, Gifu University
FullText URL poalas_033_035_039.pdf
Author Nikami, Hideki|
Abstract The facilities of laboratory animal within the Division of Animal Experiments, Life Science Research Center, Gifu University was established in April 2005. In order to gain reliable data from animal experiments, it is vital that the subjects' environment be stable and well-controlled. In addition, the safety impact to humans and bioethical rules must be considered when conducting animal experiments. We provide both the facilities necessary for such experiments as well as comprehensive support in the form of experiment program planning and subject care and maintenance.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 35
End Page 39
language Japanese
File Version publisher
Title Alternative Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis
FullText URL poalas_033_031_034.pdf
Author Aizawa, Sayaka | Higaki, Yuriko| Ogoshi, Maho| Takeuchi, Sakae| Takahashi, Sumio|
Abstract Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 31
End Page 34
language Japanese
File Version publisher
Title Alternative Structure and function of tegmentum vasculosum in avian cochlea
FullText URL poalas_033_026_030.pdf
Author Ikeda, Risa| Otono, Tsuyoshi| Ikeda, Naoya| Saito, Noboru| Ando, Motonori|
Abstract In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 26
End Page 30
language Japanese
File Version publisher
Title Alternative Taxonomic status of Aco strain derived from spiny mice
FullText URL poalas_033_022_025.pdf
Author Mochida, Hitomi| Mekada, Kazuyuki|
Abstract Spiny mice are useful in regenerative research and as a model for precocial animals. In the present study, we investigated the taxonomic status of the Aco strain, which is derived from spiny mice of an unidentified species. Although this Aco strain has been tentatively considered A. cahirinus species, it was distinguished from this species based on conventional and G-banding chromosomal patterns of this strain. Subsequently, we determined the nucleotide sequence of the mitochondorial gene cytochrome b (Cyb) and constructed a molecular phylogenetic tree. We found that the Aco strain belongs to the same clade as A. dimidiatus. Based on this molecular analysis, we concluded that the Aco strain of spiny mice should be considered A. dimidiatus.
Publication Title Proceedings of Okayama Association for Laboratory Animal Science
Published Date 2017-04
Volume volume33
Start Page 22
End Page 25
language Japanese
File Version publisher