result 10263 件
| Author | Aye Aye, Lwin| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Uchida, Akiko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11763 |
|---|---|
| Title Alternative | Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis |
| FullText URL | 001_039_050.pdf |
| Author | Nakata, Yasunari| |
| Abstract | Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed. |
| Keywords | sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 39 |
| End Page | 50 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313772 |
| Author | Harada, Daisuke| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Kuriyama, Motoaki| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11759 |
|---|---|
| Title Alternative | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| FullText URL | 007_1_009_015.pdf |
| Author | Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki| |
| Abstract | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| Keywords | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| Title Alternative | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| FullText URL | 001_029_037.pdf |
| Author | Yamamoto, Goki| |
| Abstract | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| Keywords | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 29 |
| End Page | 37 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11756 |
|---|---|
| Title Alternative | 大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 |
| FullText URL | 006_063_072.pdf |
| Author | Ito, Sekiko| Okamoto, Motoi| |
| Abstract | Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA fragments inserted into the expression vector (pGEX-4T-1) were amplified by RT-PCR using mRNA purified from E18 rat brain as template. Primers attached with BamH I or EcoR I restriction site on 5' end were used to amplify first strand cDNA by PCR. Before ligation into the pGEX-4T-1 for GST fusion protein, PCR products were once cloned using T-A cloning system because they were not directly ligated into the pGEX-4T-1. E.coli strain BL21 was transformed by pGEX-4T-1 ligated with restriction DNA fragment cut out from pCR II plasmid vector of T-A clonig system. The growth of transformed BL21 was not different between the colony incubated at 37℃ for 24-48h and the colony stored at 4℃ for 7-10 days after 24h incubation at 37℃. The desirable OD(550) of culture medium for inducing the expression of fusion protein by isopropylthio-β-D-galactoside (IPTG) was from 0.6 to 1.0, because expression of native E.coli proteins per ml of culture medium was increased relatively when IPTG was added at OD(550) more than 1.0. The expression of fusion protein reached plateau around 6h after the induction. Relative expression of native E.coli proteins per ml of culture medium increased thereafter. Therefore, it may be desirable to purify the fusion protein around 6h after the induction. |
| Keywords | phosphacan (フォスファカン) glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) BL21 IPTG fusion protein (融合蛋白) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 63 |
| End Page | 72 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313693 |
| JaLCDOI | 10.18926/11753 |
|---|---|
| Title Alternative | The effects of various fixating buffer soution in the electron microscopic observations |
| FullText URL | 006_055_061.pdf |
| Author | Akatsuka, Kazuya| |
| Abstract | 生物学的あるいは生化学的な緩衝液を使用して作製された電子顕微鏡試料と従来の電子顕微鏡用として使用されている緩衝液で作成した試料とで電子顕微鏡像から比較検討した。HEPES緩衝液で作製した試料の電子顕微鏡像は、従来の電子顕微鏡に用いられている緩衝液で作製した試料から得られた電子顕微鏡像と非常によく似た像を示し、このHEPES緩衝液は電子顕微鏡の固定用緩衝液として使用できることが認められたが、その他（PIPES、MOPS緩衝液）の緩衝液ではあまりにも細胞内の可溶性物質が溶出しすぎて使用に耐えなかった。電子顕微鏡学研究において使用されうる固定用緩衝液は細胞内のオルガネラの保持が余りにも良い場合には、その微細構造の解析が困難になり、程々に細胞内の可溶性物質が溶出した方が解析がし易くなると思われ、この様な観点から、今回使用したHEPES緩衝液は生物学的あるいは生化学的な研究から引き続き電子顕微鏡用の試料を作製する際、同じ緩衝液が使用できることが認められた。 |
| Keywords | 電子顕微鏡 (electron microscopy) 微細構造 (ultrastructure) 肝細胞 (liver cell) カコジル酸緩衝液 (cacodylate buffer) HEPES緩衝液 (HEPES buffer) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 55 |
| End Page | 61 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313640 |
| JaLCDOI | 10.18926/11751 |
|---|---|
| Title Alternative | Nursing students' perception of their acquired basic skills in nursing practices : a comparison between a new and an old curriculum |
| FullText URL | 006_047_053.pdf |
| Author | Maeda, Makiko| Kondo, Masuko| Ohta, Niwa| Ikeda, Toshiko| Nakanishi, Yoshiko| Takabatake, Harumi| Ohi, Nobuko| Takata, Setuko| |
| Abstract | 平成2年度改正の新カリキュラムで学んだ学生と旧カリキュラムで学んで学生間に、卒業時の基礎看護技術の到達度に違いがあるかを明らかにするために、看護技術の基本的要素、日常生活の援助技術、診療時の援助技術の49項目の到達度について、3年制課程の3年生に質問紙調査を行った。対象者全体では、到達度の上位10位以内に日常生活の援助技術項目の37％が含まれ、到達度の下位10位以内に診療時の援助技術項目の37％が含まれていた。新カリキュラム群と旧カリキュラム群の比較では、21項目に有意差があった。新カリキュラム群の高い15項目は日常生活の援助技術と看護技術の基本的要素に関するものが多く、旧カリキュラム群が高い6項目は診療時の援助技術が多かった。カリキュラム改正に関係なく、日常生活の援助技術の到達度は高く診療時の援助技術は低い傾向にあったが、カリキュラム改正後はその傾向が強まり、さらに看護技術の基本的要素の到達度はカリキュラム改正後に比較的高くなっていることが明らかになった。新カリキュラムでは臨地実習時間を削減しているが、基礎教育で重視する日常生活の援助技術と看護技術の基本的要素は習得できていると考えられる。 |
| Keywords | 基礎看護技術 (the basic skills in nursing practices) 到達度の学生評価 (students' perception of their acquirement) カリキュラム改正 (curriculum revision) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 47 |
| End Page | 53 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313682 |
| JaLCDOI | 10.18926/11750 |
|---|---|
| Title Alternative | Total Skin Electron Beam Therapy |
| FullText URL | 001_001_006.pdf |
| Author | Nakagiri, Yoshitada| Inamura, Keiji| Miyake, Hideaki| Tahara, Seiji| Mimura, Seiichi| Egusa, Tomomi| Mikami, Yasutaka| Yamada, Toshiharu| Sugita, Katsuhiko| Hiraki, Yoshio| |
| Abstract | The peripherally T-cell lymphoma; Mycosis fungoides etc, has the good radiation sensitivity, and has been adapted for total skin electron beam therapy. In this study the pendular irradiation method was used for the purpose of total skin electron beam therapy in Mycosis fungoides, and physical data on the radiation field and the electron beam energy were useful clinically. |
| Keywords | 電子線全身照射法 菌状息肉症 全身性皮膚疾患 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313887 |
| JaLCDOI | 10.18926/11749 |
|---|---|
| Title Alternative | メキシコ肺吸虫のセルカリアはどれか？ |
| FullText URL | 010_2_063_067.pdf |
| Author | Tongu, Yasumasa| |
| Abstract | The true cercaria of Paragonimus mexicanus is the cercaria reported by Malek et al. in 1985 as a cercaria of P. peruvianus. However, there is a question whether the flame cell formula of 2〔(3+3+3+3+3)+(3+3+3+3+3)〕=60 is justified. Therefore, I question the possibility of the cercaria of P. mexicanus reported by Ito et al. in 1985. If it has a pseudo-sucker, it belongs to another species of Paragonimus cercaria. The cercaria reported by Tongu et al. in 1990 from Venezuela was eliminated the possibility of a new species. It is probable that this cercaria belongs to P. mexicanus. Ibanez revived the name of P. peruvianus in 1990 by the differences in the cercaria and adults. However, I disagree with his opinion. |
| Keywords | Paragonimus mexicanus (メキシコ肺吸虫) Paragonimus peruvianus (ペルー肺吸虫) cercaria (セルカリア) taxonomy (分類) Latin America (中南米) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 2000-03-24 |
| Volume | volume10 |
| Issue | issue2 |
| Start Page | 63 |
| End Page | 67 |
| ISSN | 1345-0948 |
| language | English |
| File Version | publisher |
| NAID | 120002313440 |
| JaLCDOI | 10.18926/11748 |
|---|---|
| Title Alternative | Dietary treatment for the aged (A study on dietary treatments for the aged conducted at the time of leaving hospital and on the results of interviews with discharged patients) |
| FullText URL | 006_041_045.pdf |
| Author | Nakanishi, Yoshiko| Kondo, Masuko| Ota, Niwa| Inoshita, Hikari| Ikeda, Toshiko| Takata, Setuko| |
| Abstract | 高齢者を対象とした退院指導の評価を行う目的で、70歳以上の外来通院患者を対象として、患者が受けた退院指導の内容、理解度、実践状況の3つの要素について分析を行った。その中から、特に食事指導に焦点をあて在宅療養の視点から退院指導の問題点を考察した。食事指導を受けて退院した患者の約半数は更に指導を希望していた。指示された指導が実践できない患者の理由は、(1)指導不足、(2)意欲の喚起不足、(3)協力者の知識不足が上げられた。患者が希望している指導の内容は、食事療法の指導だけでなく、治療食や老人に適した食事についてであった。特に、栄養価の高い食事への関心が高く、具体的な指導を希望していた。また、看護婦による、社会資源を活用すべきかどうか、訪問看護を適応すべきかどうかの判断が、退院に向けてのアセスメントの内容として問題となった。 |
| Keywords | 高齢者 (the aged) 在宅療養 (home treatment) 退院指導 (discharge-care) 食事指導 (dietary treatment) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 41 |
| End Page | 45 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313691 |
| Author | Okamoto, Yuko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Yamashita, Toru| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Ohta, Yasuyuki| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Wu, Yumei| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Hamasaki, Ichiro| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11736 |
|---|---|
| Title Alternative | Percentage body fat and results of a periodic health examination |
| FullText URL | 006_023_028.pdf |
| Author | Ohta, Takeo| Goto, Akio| Masaki, Hisako| Takami, Yoko| |
| Abstract | 肥満や栄養過剰は多くの慢性疾患、とりわけ成人病と関連が強いことが従来から指摘されている。肥満の判定には体脂肪率を測定することが重要であるが、その方法の一つとして、集団検診に使用しやすい生体電気インピーダンス法（bioelectrical impedance analysis）がある。今回、1ビール製造工場の定期健康診断において、対象者男235人および女137人の体脂肪率をこの方法により測定した。まずこの体脂肪率とBMIおよび肥満度との相関関係を求め、男性より女性において相関が高いことを認めた。ついで肥満群と正常群に分けて、血圧および血液検査の異常率の出現頻度を統計的に比較したところ、肥満群では男女共に総コレステロールで、また男性では尿酸、γGTP、SGPTの各値で、異常者が高率であることを認めた。またSGPTは30才台から、γGTP及びTchは40才台からその傾向が見られた。しかし、肥満指数とこれらの検査値との相関係数は高くはなかった。 |
| Keywords | 体脂肪率 (percentage body fat) インピーダンス (bioelectorical impedance) BIA 健康診断 (periodic health examination) BMI |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 23 |
| End Page | 28 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313771 |
| JaLCDOI | 10.18926/11732 |
|---|---|
| Title Alternative | Interpersonal values in 4-year course nursing students : Comparisons with 3-year course nursing students and students not majoring in nursing science |
| FullText URL | 010_1_029_034.pdf |
| Author | Nagata, Hiroshi| Kato, Kumiko| Sasano, Kanji| |
| Abstract | 1999年4年制看護課程に入学した看護学生の入学時の対人関係価値をKG-SIVによって現実の自己像とナースの理想像について調査し，これを同年に非看護課程に入学した学生と3年制看護課程に異なる時期（1990年，1993年，1996年）に入学した看護学生3コホートと比較した。その結果，4年制看護学生は非看護学生に比べ，「博愛」と「同調」を重視する程度が強く，「支持」と「承認」，「独立」を重視する程度が弱かった。また，彼女ら の対人関係価値は3年制看護課程の学生のそれに類似していた。さらに，コホートの年次的移行のなかで看護学生の対人関係価値は「支持」と「独立」への動機づけが増大し，「博愛」への動機づけが滅退する傾向を見せた。 |
| Keywords | 対人関係価値 (interpersonal values) 看護学生 (nursing students) コホート (cohorts) |
| Publication Title | 岡山大学医学部保健学科紀要 |
| Published Date | 1999-12-20 |
| Volume | volume10 |
| Issue | issue1 |
| Start Page | 29 |
| End Page | 34 |
| ISSN | 1345-0948 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314023 |
