result 533 件
| JaLCDOI | 10.18926/11921 |
|---|---|
| Title Alternative | Comparative studies of proliferating cell nuclear antigen and bromodeoxyuridine staining pattern in rat regenerating liver cells |
| FullText URL | 005_079_085.pdf |
| Author | Sakiyama, Junko| Mori, Shuji| Ichimura, Mituko| Tohge, Hiroko| Okamoto, Motoi| Endo, Hiroshi| |
| Abstract | 種々の条件下（10％緩衝ホルマリン・6時間、1％パラホルムアルデヒド・6時間、100％メタノール・4時間、70％エタノール・6時間、100％アセトン・2時間、カルノア液・4時間）で固定したラット再生肝組織に免疫組織染色を施し、Proliferating cell nuclear antigen （PCNA）の核内染色パターンについての検討を行った。その結果、100％メタノール及び70％エタノールで固定した場合には、明瞭な顆粒状の染色像が得られた。10％緩衝ホルマリン及び1％パラホルムアルデヒドの場合には、明瞭な顆粒状並びに弱いびまん性の染色像が得られた。S期に対する代表的なマーカーとして知られるBromodeoxyuridine （BrdU）の染色像との比較のもと、再生肝組織中のPCNA陽性細胞の数とBrdU陽性細胞の数を再生ピーク時の術後1日目で検討したところ、100％メタノール及び70％エタノールで固定した際の陽性細胞数が、最もBrdUの所見と一致した。しかし、10％緩衝ホルマリン、1％パラホルムアルデヒド、100％アセトン、カルノア液で固定した場合には、BrdUの所見と著しい解離が見られた。これらの知見は、PCNAを指標に細胞増殖を評価する上で、100％メタノール、70％エタノールでの固定が有用であることを示唆する。 |
| Keywords | PCNA BrdU 免疫組織染色 (immunohistochemical staining) 再生肝 (regenerating liver) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1995-01-31 |
| Volume | volume5 |
| Start Page | 79 |
| End Page | 85 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313417 |
| JaLCDOI | 10.18926/11875 |
|---|---|
| Title Alternative | Ferrous Iron-induced Lipid Peroxidation in Cell Culture: Effects of Ferrous and Ferric Irons on Cell Survival and Serum Lipid Peroxide |
| FullText URL | 004_061_068.pdf |
| Author | Yamamoto, Goki| |
| Abstract | Cell killing activities of ferrous and ferric irons (Fe(2+), Fe(3+)) in cultured 3T3 cells were investigated by the colony forming method in the culture medium containing 10% calf serum. In the colony forming method, small isolated colonies are seen in the dish which are the resulted of individual cells having undergone a series of cell divisions. Survival curve for Fe(2+)-induced loss of reproductive capacity (cell death) in 3T3 cells, showed a shoulder at low concentrations and only became exponential at high concentrations. Cells affected at 0.05mM Fe(2+) underwent apparently normal cell divisions, but at a much slower rate than the control. Survival rate at 0.5mM Fe(2+), which iron was added after seeding cells (added group), showed 0.75% but that at 0.5mM Fe(3+) did 19.3%. From the determination of Fe(2+) content carried out by the colorimetric method used N-PSAP, the oxidation of Fe(2+) was seen rapidly after adding Fe(2+) into the culture medium. In the cases of cells seeded 3 hours after adding Fe(2+) or Fe(3+) (preadded group), the survival rates showed much lower than those of the added groups, that is 55% at 0.5mM Fe(2+) and 89% at 0.5mM Fe(3+). The facts suggest that the radical reactions iniciated by the oxidation of Fe(2+) under the presence of cells, occured just after adding Fe(2+), play a crucial role in the damages on the adhesion of cells onto the dish and the following proliferation of cells. Serum lipid peroxides, as thiobarbituric acid reactive substances (TBA value), of the final cultured medium at colony counting time were measured. In the Fe(2+) added group, the TBA values more increased in proportion to the added concentrations of Fe(2+), whereas in the Fe(3+) added group, the increments of TBA values were very slightly and not to be depended on the concentrations. Furthermore, the TBA values of the Fe(2+) or Fe(3+) preadded groups were estimated as well as those of the added groups, respectively. The facts suggest that serum lipid peroxides induced by Fe(2+) have little effect on the proliferation capacity of cells in the level of cell culture. |
| Keywords | Cell Survival Ferrous and Ferric Irons Serum Lipid Peroxide |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1994-01-31 |
| Volume | volume4 |
| Start Page | 61 |
| End Page | 68 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313910 |
| Author | Rodriguez Andrea, Paola| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Uchida, Akiko| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Harada, Daisuke| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11759 |
|---|---|
| Title Alternative | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| FullText URL | 007_1_009_015.pdf |
| Author | Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki| |
| Abstract | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| Keywords | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313519 |
| Author | Ohta, Yasuyuki| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| JaLCDOI | 10.18926/11693 |
|---|---|
| Title Alternative | Hyhrid vascular grafts and one of our approaches |
| FullText URL | 061_114_122.pdf |
| Author | Noishiki, Yasuharu| |
| Abstract | 人工血管により高度な機能をもたす目的で，ハイブリット化が今日進められるようになってきた。この背景について説明するとともに，我々の独自に行っているハイブリット型人工血管作成の方法およびその成果について報告する。その具体的方法としては以下の通りである。末梢静脈小片を勇刀にて細切したのち，約20ccの生理的食塩水に入れて静脈組織細切片浮遊液を作った。次に高有孔性人工血管の一端より吸引管を挿入し，これを作成した液に入れ，吸引によって組織片を外側から人工血管壁に絡ませた。次に新鮮な血液を注ぎ，組織片をさらに固着させた。成犬胸部下行大動脈へこのような処理をしたポリエステル布製人工血管を植え込んだところ，植え込み5日目に新生血管壁内部に無数の内皮細胞の増殖像がみられ，35日目の例では吻合部はもとより，人工血管の中央部ですら内皮細胞による完全な被覆を認めた。 以上のような方法で新生血管壁が容易に形成されることが判明したことより，今後，他の人工臓器分野でもハイブリット化が予測される。 |
| Keywords | 人工血管 (Vascular graft) ハイブリット (Hybrid) 新生内膜 (Neointima) 内皮細胞 (Endothelial cell) 自家移植 (Autologous transplantation) |
| Publication Title | 環境病態研報告 |
| Published Date | 1990-09 |
| Volume | volume61 |
| Start Page | 114 |
| End Page | 122 |
| ISSN | 0913-3771 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313776 |
| JaLCDOI | 10.18926/11687 |
|---|---|
| Title Alternative | New trend of the development of vascular prostheses |
| FullText URL | 061_098_113.pdf |
| Author | Noishiki, Yasuharu| |
| Abstract | 人工血管の開発研究における動向について，我々の行ってきた研究をまじえて世界の動きを解析した。近年の砺究の流れは過去1世紀にわたる試行錯誤の上にあって，さらに大きく揺れ動いている。人工血管の内面に必要な抗血栓性の獲得においても，設計者によって考えを異にし，米国では，人工心臓に用いる抗血栓性合性高分子材料をもってそれに当てようとしているものが多い。我々はあくまで宿主のもつ諸機能を引き出し，自然の動きの一つとしての治癒を無理なく導いて，内皮細胞によって内面を覆わせる方法を採用している。そのいくつかの例として，超極細ポリエステル繊維製人工血管，結合組織管，バイオマトリックス人工血管，ヘパリン化生体組織由来人工血管，成長できる人工血管などについて解説した。 |
| Keywords | 人工血管 (Vascular prosthesis) 新生内膜 (Neointima) 内皮細胞 (Endothelial cells) 抗血栓性 (Antithrombogenicity) |
| Publication Title | 環境病態研報告 |
| Published Date | 1990-09 |
| Volume | volume61 |
| Start Page | 98 |
| End Page | 113 |
| ISSN | 0913-3771 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313859 |
| Author | Ohtani, Yu| |
|---|---|
| Published Date | 2007-03-23 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Yuhara, Masataka| |
|---|---|
| Published Date | 1995-02-01 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume84 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Murakami, Kenji| Nishioka, Junko| Matsubara, Sachiko| |
|---|---|
| Published Date | 1998-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume87 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Fujio, Hideki| |
|---|---|
| Published Date | 2006-09-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Han, Song| Abe, Asaki| Narahara, Kiyoaki| Kondo, Yasuhiro| |
|---|---|
| Published Date | 2007-02 |
| Publication Title | 岡山大学農学部学術報告 |
| Volume | volume96 |
| Issue | issue1 |
| Content Type | Departmental Bulletin Paper |
| Author | Takata, Hidehiko| |
|---|---|
| Published Date | 2004-03-31 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Suzuki, Mayumi| |
|---|---|
| Published Date | 2005-06-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Shen, Zheng-Nan| |
|---|---|
| Published Date | 2005-06-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Koyama, Maho| |
|---|---|
| Published Date | 2004-03-25 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | 大水 総一| |
|---|---|
| Published Date | 1998-03-25 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | 牧野 泰裕| |
|---|---|
| Published Date | 1988-03-31 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
