| ID | 16826 |
| Eprint ID | 16826
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| FullText URL | |
| Title Alternative | Malignant transformation of diploid rat liver cells treated with 3'Me-DAB in tissue culture
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| Author |
Shiraishi, Masayuki
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| Abstract | 正二倍体性単個クローンを使用し, 3'-Me-DABによる正常ラット肝細胞癌化の可能性を追求した.1)5γ/mlないしは80γ/ml 3'-Me-DAB,140日間連続処理により,細胞の癌化を認め,以降, 3'-Me-DAB処理後, 3'-Me-DABを含まない正常培地に置き換えられた系でも癌化を認めた.尚対照群では, 3'-Me-DAB処理細胞の殆んどに癌化を確認した時点の232日迄に自然癌化は,認めなかった.2)癌化過程における3'-Me-DAB処理細胞の特性の変化としては,形態異常染色体上の変化, 3'-Me-DABに対する耐性の獲得などが認められたが,増殖性,血清要求性については対照群との差は認められなかった.
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| Abstract Alternative | A diploid clone derived from rat liver cells was treated in culture with 3'Me-DAB. The cells treated for 140 days with 5 μg/ml or 80 μg/ml of 3'Me-DAB became malignant. Cells cultured in normal medium after treatment with 3'Me-DAB also became malignant. Untreated control cells did not produce tumors on back-transplantation at the 232nd day stage, when 3'Me-DAB-treated cells were confirmed to be malignant. The transformed cells showed some morphological and chromosomal changes, as well as resistance to the toxic effects of 3'Me-DAB. However, no definite differences in growth potential and serum requirement were found between treated and untreated-control cells.
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| Keywords | 単個培養
正二倍体性
3'-Me-DAB
癌化
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| Published Date | 1979-04-30
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| Publication Title |
岡山医学会雑誌
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| Publication Title Alternative | Journal of Okayama Medical Association
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| Volume | volume91
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| Issue | issue3-4
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| Publisher | 岡山医学会
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| Publisher Alternative | Okayama Medical Association
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| Start Page | 407
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| End Page | 414
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| ISSN | 0030-1558
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| NCID | AN00032489
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| Content Type |
Journal Article
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| Official Url | https://www.jstage.jst.go.jp/article/joma1947/91/3-4/91_3-4_407/_article/-char/ja/
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| Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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| language |
Japanese
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| Copyright Holders | 岡山医学会
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| File Version | publisher
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| Refereed |
True
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| Eprints Journal Name | joma
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