start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=12 article-no= start-page=4932 end-page=4951 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20241021 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The leucine-rich repeat receptor kinase QSK1 regulates PRR-RBOHD complexes targeted by the bacterial effector HopF2Pto en-subtitle= kn-subtitle= en-abstract= kn-abstract=Plants detect pathogens using cell-surface pattern recognition receptors (PRRs) such as ELONGATION Factor-TU (EF-TU) RECEPTOR (EFR) and FLAGELLIN SENSING 2 (FLS2), which recognize bacterial EF-Tu and flagellin, respectively. These PRRs belong to the leucine-rich repeat receptor kinase (LRR-RK) family and activate the production of reactive oxygen species via the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD). The PRR-RBOHD complex is tightly regulated to prevent unwarranted or exaggerated immune responses. However, certain pathogen effectors can subvert these regulatory mechanisms, thereby suppressing plant immunity. To elucidate the intricate dynamics of the PRR-RBOHD complex, we conducted a comparative coimmunoprecipitation analysis using EFR, FLS2, and RBOHD in Arabidopsis thaliana. We identified QIAN SHOU KINASE 1 (QSK1), an LRR-RK, as a PRR-RBOHD complex-associated protein. QSK1 downregulated FLS2 and EFR abundance, functioning as a negative regulator of PRR-triggered immunity (PTI). QSK1 was targeted by the bacterial effector HopF2Pto, a mono-ADP ribosyltransferase, reducing FLS2 and EFR levels through both transcriptional and transcription-independent pathways, thereby inhibiting PTI. Furthermore, HopF2Pto transcriptionally downregulated PROSCOOP genes encoding important stress-regulated phytocytokines and their receptor MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2. Importantly, HopF2Pto requires QSK1 for its accumulation and virulence functions within plants. In summary, our results provide insights into the mechanism by which HopF2Pto employs QSK1 to desensitize plants to pathogen attack. en-copyright= kn-copyright= en-aut-name=GotoYukihisa en-aut-sei=Goto en-aut-mei=Yukihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KadotaYasuhiro en-aut-sei=Kadota en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MbengueMalick en-aut-sei=Mbengue en-aut-mei=Malick kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LewisJennifer D en-aut-sei=Lewis en-aut-mei=Jennifer D kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsuiHidenori en-aut-sei=Matsui en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MakiNoriko en-aut-sei=Maki en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NgouBruno Pok Man en-aut-sei=Ngou en-aut-mei=Bruno Pok Man kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SklenarJan en-aut-sei=Sklenar en-aut-mei=Jan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=DerbyshirePaul en-aut-sei=Derbyshire en-aut-mei=Paul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShibataArisa en-aut-sei=Shibata en-aut-mei=Arisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IchihashiYasunori en-aut-sei=Ichihashi en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=GuttmanDavid S en-aut-sei=Guttman en-aut-mei=David S kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=NakagamiHirofumi en-aut-sei=Nakagami en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=SuzukiTakamasa en-aut-sei=Suzuki en-aut-mei=Takamasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=MenkeFrank L H en-aut-sei=Menke en-aut-mei=Frank L H kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=RobatzekSilke en-aut-sei=Robatzek en-aut-mei=Silke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=DesveauxDarrell en-aut-sei=Desveaux en-aut-mei=Darrell kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=ZipfelCyril en-aut-sei=Zipfel en-aut-mei=Cyril kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=ShirasuKen en-aut-sei=Shirasu en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= affil-num=1 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=2 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=3 en-affil=The Sainsbury Laboratory, University of East Anglia kn-affil= affil-num=4 en-affil=Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto kn-affil= affil-num=5 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=6 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=7 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=8 en-affil=The Sainsbury Laboratory, University of East Anglia kn-affil= affil-num=9 en-affil=The Sainsbury Laboratory, University of East Anglia kn-affil= affil-num=10 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=11 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= affil-num=12 en-affil=Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto kn-affil= affil-num=13 en-affil=Plant Proteomics Research Unit, RIKEN CSRS kn-affil= affil-num=14 en-affil=College of Bioscience and Biotechnology, Chubu University kn-affil= affil-num=15 en-affil=The Sainsbury Laboratory, University of East Anglia kn-affil= affil-num=16 en-affil=The Sainsbury Laboratory, University of East Anglia kn-affil= affil-num=17 en-affil=Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto kn-affil= affil-num=18 en-affil=Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich kn-affil= affil-num=19 en-affil=Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS) kn-affil= END start-ver=1.4 cd-journal=joma no-vol=22 cd-vols= no-issue=6 article-no= start-page=271 end-page=285 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=2024 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effects of Sediment Microbial Fuel Cells on CH4 and CO2 Emissions from Straw Amended Paddy Soil en-subtitle= kn-subtitle= en-abstract= kn-abstract=Straw returning into paddy soil enhances soil organic matter which usually promotes the emission of greenhouse gases to the atmosphere. The application of sediment microbial fuel cells (SMFCs) to paddy soil activates power-generating microorganisms and enhances organic matter biodegradation. In the present study, rice straw addition in SMFCs was examined to determine its effect on CH4 and CO2 emissions. Columns (height, 25 cm; inner diameter, 9 cm) with four treatments: soil without and with rice straw under SMFC and without SMFC conditions were incubated at 25°C for 70 days. Anodic potential values at 7 cm depth sediment were kept higher by SMFCs than those without SMFCs. Cumulative CH4 emission was significantly reduced by SMFC with straw amendment (p < 0.05) with no significant effect on CO2 emission. 16S rRNA gene analysis results showed that Firmicutes at the phylum, Closteridiales and Acidobacteriales at order level were dominant on the anode of straw-added SMFC, whereas Methanomicrobiales were in the treatment without SMFC, indicating that a certain group of methanogens were suppressed by SMFC. Our results suggest that the anodic redox environment together with the enrichment of straw-degrading bacteria contributed to a competitive advantage of electrogenesis over methanogenesis in straw-added SMFC system. en-copyright= kn-copyright= en-aut-name=BekeleAdhena Tesfau en-aut-sei=Bekele en-aut-mei=Adhena Tesfau kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaMorihiro en-aut-sei=Maeda en-aut-mei=Morihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AkaoSatoshi en-aut-sei=Akao en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SomuraHiroaki en-aut-sei=Somura en-aut-mei=Hiroaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakanoChiyu en-aut-sei=Nakano en-aut-mei=Chiyu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NishinaYuta en-aut-sei=Nishina en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Faculty of Science and Engineering, Doshisha University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Organization for Research Strategy and Development, Okayama University kn-affil= affil-num=6 en-affil=Research Institute for Interdisciplinary Science, Okayama University kn-affil= en-keyword=straw kn-keyword=straw en-keyword=methane mitigation kn-keyword=methane mitigation en-keyword=SMFC kn-keyword=SMFC en-keyword=microorganisms kn-keyword=microorganisms en-keyword=current generation kn-keyword=current generation END start-ver=1.4 cd-journal=joma no-vol=351 cd-vols= no-issue= article-no= start-page=199522 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202501 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evidence for the replication of a plant rhabdovirus in its arthropod mite vector en-subtitle= kn-subtitle= en-abstract= kn-abstract=Transmission of plant viruses that replicate in the insect vector is known as persistent-propagative manner. However, it remains unclear whether such virus-vector relationships also occur between plant viruses and other biological vectors such as arthropod mites. In this study, we investigated the possible replication of orchid fleck virus (OFV), a segmented plant rhabdovirus, within its mite vector (Brevipalpus californicus s.l.) using quantitative RT-qPCR, western blotting and next-generation sequencing. Time-course RT-qPCR and western blot analyses showed an increasing OFV accumulation pattern in mites after virus acquisition. Since OFV genome expression requires the transcription of polyadenylated mRNAs, polyadenylated RNA fractions extracted from the viruliferous mite samples and OFV-infected plant leaves were used for RNA-seq analysis. In the mite and plant datasets, a large number of sequence reads were aligned to genomic regions of OFV RNA1 and RNA2 corresponding to transcribed viral gene mRNAs. This includes the short polyadenylated transcripts originating from the leader and trailer regions at the ends of the viral genome, which are believed to play a crucial role in viral transcription/replication. In contrast, a low number of reads were mapped to the non-transcribed regions (gene junctions). These results strongly suggested that OFV gene expression occurs both in mites and plants. Additionally, deep sequencing revealed the accumulation of OFV-derived small RNAs in mites, although their size profiles differ from those found in plants. Taken together, our results indicated that OFV replicates within a mite vector and is targeted by the RNA-silencing mechanism. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujitaMiki en-aut-sei=Fujita en-aut-mei=Miki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TelengechPaul en-aut-sei=Telengech en-aut-mei=Paul kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MaruyamKazuyuki en-aut-sei=Maruyam en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HyodoKiwamu en-aut-sei=Hyodo en-aut-mei=Kiwamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TassiAline Daniele en-aut-sei=Tassi en-aut-mei=Aline Daniele kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OchoaRonald en-aut-sei=Ochoa en-aut-mei=Ronald kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AndikaIda Bagus en-aut-sei=Andika en-aut-mei=Ida Bagus kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SuzukiNobuhiro en-aut-sei=Suzuki en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=6 en-affil=Tropical Research and Education Center, University of Florida kn-affil= affil-num=7 en-affil=Systematic Entomology Laboratory, USDA kn-affil= affil-num=8 en-affil=College of Plant Protection, Northwest A&F University kn-affil= affil-num=9 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= en-keyword=Rhabdovirus kn-keyword=Rhabdovirus en-keyword=Plant kn-keyword=Plant en-keyword=Mite kn-keyword=Mite en-keyword=Vector kn-keyword=Vector en-keyword=Replication kn-keyword=Replication en-keyword=mRNA kn-keyword=mRNA en-keyword=Small RNA kn-keyword=Small RNA END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250603 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Amino Acid Substitutions in Loop C of Arabidopsis PIP2 Aquaporins Alters the Permeability of CO2 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The transport of CO2 across biomembranes in plant cells is essential for efficient photosynthesis. Some aquaporins capable of CO2 transport, referred to as ‘COOporins’, are postulated to play a crucial role in leaf CO2 diffusion. However, the structural basis of CO2 permeation through aquaporins remains largely unknown. Here, we show that amino acids in loop C are critical for the CO2 permeability of Arabidopsis thaliana PIP2 aquaporins. We found that swapping tyrosine and serine in loop C to histidine and phenylalanine, which differ between AtPIP2;1 and AtPIP2;3, altered CO2 permeability when examined in the Xenopus laevis oocyte heterologous expression system. AlphaFold2 modelling indicated that these substitution induced a conformational shift in the sidechain of arginine in the aromatic/arginine (ar/R) selectivity filter and in lysine at the extracellular mouth of the monomeric pore in PIP2 aquaporins. Our findings demonstrate that distal amino acid substitutions can trigger conformational changes of the ar/R filter in the monomeric pore, modulating CO2 permeability. Additionally, phylogenetic analysis suggested that aquaporins capable of dual water/CO2 permeability are ancestral to those that are water-selective and CO2-impermeable, and CO2-selective and water impermeable. en-copyright= kn-copyright= en-aut-name=TaniaShaila Shermin en-aut-sei=Tania en-aut-mei=Shaila Shermin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UtsugiShigeko en-aut-sei=Utsugi en-aut-mei=Shigeko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsuchiyaYoshiyuki en-aut-sei=Tsuchiya en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasanoShizuka en-aut-sei=Sasano en-aut-mei=Shizuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatsuharaMaki en-aut-sei=Katsuhara en-aut-mei=Maki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=Arabidopsis thaliana kn-keyword=Arabidopsis thaliana en-keyword=CO2 transport kn-keyword=CO2 transport en-keyword=monomeric pore kn-keyword=monomeric pore en-keyword=PIP2 aquaporin kn-keyword=PIP2 aquaporin en-keyword=Xenopus laevis kn-keyword=Xenopus laevis END start-ver=1.4 cd-journal=joma no-vol=177 cd-vols= no-issue=4 article-no= start-page=e70396 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202507 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=CNGC2 Negatively Regulates Stomatal Closure and Is Not Required for flg22- and H2O2-Induced Guard Cell [Ca2+]cyt Elevation in Arabidopsis thaliana en-subtitle= kn-subtitle= en-abstract= kn-abstract=In guard cells, cytosolic Ca2+ acts as a second messenger that mediates abscisic acid (ABA)- and pathogen-associated molecular pattern (PAMP)-induced stomatal closure. It was reported that Arabidopsis cyclic nucleotide-gated ion channel 2 (CNGC2) functions as hydrogen peroxide (H2O2)- and PAMP-activated Ca2+-permeable channels at the plasma membrane of mesophyll cells and mediates Ca2+-dependent PAMP-triggered immunity. In this study, we examined the role of CNGC2 in the regulation of stomatal movement because CNGC2 is also expressed in guard cells. We found that stomata of the CNGC2 disruption mutant cngc2-3 are constitutively closed even in the absence of ABA or the flagellar-derived PAMP, flg22. Consistently, leaf temperatures of the cngc2-3 mutant were higher than those of wild-type (WT) plants. The stomatal phenotype of the cngc2-3 mutant was restored by complementation with wild-type CNGC2 under the control of the guard cell preferential promoter, pGC1. Elevation of cytosolic free Ca2+ concentration in guard cells induced by flg22 and H2O2 remained intact in the cngc2-3 mutant. The introduction of the ost1-3 mutation into the cngc2-3 background did not alter the stomatal phenotype. However, the stomatal phenotype of the cngc2-3 mutant was successfully rescued in the double disruption mutant cngc2-3aba2-2. Taken together, these results suggest that CNGC2 negatively regulates stomatal closure response and does not function as flg22– and H2O2-activated Ca2+ channels in guard cells. Though CNGC2 is responsive for H2O2- and flg22-induced [Ca2+]cyt elevation in mesophyll cells, the involvement of CNGC2 in the response to H2O2 and flg22 in guard cells is questionable. en-copyright= kn-copyright= en-aut-name=AkterRojina en-aut-sei=Akter en-aut-mei=Rojina kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=InoueYasuhiro en-aut-sei=Inoue en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MasumotoSaori en-aut-sei=Masumoto en-aut-mei=Saori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MimataYoshiharu en-aut-sei=Mimata en-aut-mei=Yoshiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsuuraTakakazu en-aut-sei=Matsuura en-aut-mei=Takakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakamuraToshiyuki en-aut-sei=Nakamura en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakamuraYoshimasa en-aut-sei=Nakamura en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MurataYoshiyuki en-aut-sei=Murata en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=MunemasaShintaro en-aut-sei=Munemasa en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Faculty of Agriculture, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil= kn-affil= affil-num=7 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=8 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=9 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=10 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=calcium signaling kn-keyword=calcium signaling en-keyword=CNGC kn-keyword=CNGC en-keyword=stomata kn-keyword=stomata END start-ver=1.4 cd-journal=joma no-vol=301 cd-vols= no-issue=7 article-no= start-page=110291 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202507 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A repertoire of visible light–sensitive opsins in the deep-sea hydrothermal vent shrimp Rimicaris hybisae en-subtitle= kn-subtitle= en-abstract= kn-abstract=Unlike terrestrial environments, where humans reside, there is no sunlight in the deep sea. Instead, dim visible light from black-body radiation and bioluminescence illuminates hydrothermal vent areas in the deep sea. A deep-sea hydrothermal vent shrimp, Rimicaris hybisae, is thought to detect this dim light using its enlarged dorsal eye; however, the molecular basis of its photoreception remains unexplored. Here, we characterized the molecular properties of opsins, universal photoreceptive proteins in animals, found in R. hybisae. Transcriptomic analysis identified six opsins: three Gq-coupled opsins, one Opn3, one Opn5, and one peropsin. Functional analysis revealed that five of these opsins exhibited light-dependent G protein activity, whereas peropsin exhibited the ability to convert all-trans-retinal to 11-cis-retinal like photoisomerases. Notably, all the R. hybisae opsins, including Opn5, convergently show visible light sensitivity (around 457–517 nm), whereas most opsins categorized as Opn5 have been demonstrated to be UV sensitive. Mutational analysis revealed that the unique visible light sensitivity of R. hybisae Opn5 is achieved through the stabilization of a protonated Schiff base by a counterion residue at position 83 (Asp83), which differs from the position identified in other opsins. These findings suggest that the vent shrimp R. hybisae has adapted its photoreceptive devices to dim deep-sea hydrothermal light by selectively maintaining a repertoire of visible light–sensitive opsins, including the uniquely tuned Opn5. en-copyright= kn-copyright= en-aut-name=NagataYuya en-aut-sei=Nagata en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyamotoNorio en-aut-sei=Miyamoto en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SatoKeita en-aut-sei=Sato en-aut-mei=Keita kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishimuraYosuke en-aut-sei=Nishimura en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaniokaYuki en-aut-sei=Tanioka en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YamanakaYuji en-aut-sei=Yamanaka en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YoshizawaSusumu en-aut-sei=Yoshizawa en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakahashiKuto en-aut-sei=Takahashi en-aut-mei=Kuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ObayashiKohei en-aut-sei=Obayashi en-aut-mei=Kohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TsukamotoHisao en-aut-sei=Tsukamoto en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=TakaiKen en-aut-sei=Takai en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=YamashitaTakahiro en-aut-sei=Yamashita en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=SudoYuki en-aut-sei=Sudo en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=KojimaKeiichi en-aut-sei=Kojima en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) kn-affil= affil-num=3 en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Research Center for Bioscience and Nanoscience (CeBN), Research Institute for Marine Resources Utilization, Japan Agency for Marine-Earth Science and Technology (JAMSTEC) kn-affil= affil-num=5 en-affil=School of Pharmaceutical Sciences, Okayama University kn-affil= affil-num=6 en-affil=School of Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Atmosphere and Ocean Research Institute, The University of Tokyo kn-affil= affil-num=8 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=9 en-affil=Department of Biology, Graduate School of Science, Kobe University kn-affil= affil-num=10 en-affil=Department of Biology, Graduate School of Science, Kobe University kn-affil= affil-num=11 en-affil=Institute for Extra-Cutting-Edge Science and Technology Avant-Garde Research (X-Star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC) kn-affil= affil-num=12 en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=13 en-affil=Department of Biophysics, Graduate School of Science, Kyoto University kn-affil= affil-num=14 en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=15 en-affil=Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=rhodopsin kn-keyword=rhodopsin en-keyword=opsin kn-keyword=opsin en-keyword=G protein–coupled receptor kn-keyword=G protein–coupled receptor en-keyword=signal transduction kn-keyword=signal transduction en-keyword=photoreceptor kn-keyword=photoreceptor en-keyword=vision kn-keyword=vision en-keyword=photobiology kn-keyword=photobiology en-keyword=vent shrimp kn-keyword=vent shrimp en-keyword=deep sea kn-keyword=deep sea en-keyword=molecular evolution kn-keyword=molecular evolution END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250612 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Sulfur dioxide-induced guard cell death and stomatal closure are attenuated in nitrate/proton antiporter AtCLCa mutants en-subtitle= kn-subtitle= en-abstract= kn-abstract=Guard cells surrounding the stomata play a crucial role in regulating the entrance of hazardous gases such as SO2 into leaves. Stomatal closure could be a plant response to mitigate SO2 damage, although the mechanism for SO2-induced closure remains controversial. Proposed mediators for SO2-induced stomatal closure include phytohormones, reactive oxygen species, gasotransmitters, and cytosolic acidification. In this study, we investigated the mechanism of stomatal closure in Arabidopsis in response to SO2. Despite an increment in auxin and jasmonates after SO2 exposure, the addition of auxin did not cause stomatal closure and jasmonate-insensitive mutants exhibited SO2-induced stomatal closure suggesting auxin and jasmonates are not mediators leading to the closure. In addition, supplementation of scavenging reagents for reactive oxygen species and gasotransmitters did not inhibit SO2-induced closure. Instead, we found that cytosolic acidification is a credible mechanism for SO2-induced stomatal closure in Arabidopsis. CLCa mutants coding H+/nitrate antiporter, involved in cytosolic pH homeostasis, showed less sensitive stomatal phenotype against SO2. These results suggest that cytosolic pH homeostasis plays a tenable role in SO2 response in guard cells. en-copyright= kn-copyright= en-aut-name=OoiLia en-aut-sei=Ooi en-aut-mei=Lia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuuraTakakazu en-aut-sei=Matsuura en-aut-mei=Takakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=airborne pollutants kn-keyword=airborne pollutants en-keyword=cytosolic acidification kn-keyword=cytosolic acidification en-keyword=stomatal closure kn-keyword=stomatal closure en-keyword=sulfur dioxide kn-keyword=sulfur dioxide END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=稲わら施用水田土壌からのCH4およびCO2 排出抑制に向けた底質微生物燃料電池の開発 kn-title=Development of sediment microbial fuel cells to reduce CH4 and CO2 emissions from straw-amended paddy soil en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ADHENA TESFAU BEKELE en-aut-sei=ADHENA TESFAU BEKELE en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=生物学的製剤の使用は、関節リウマチに対する整形外科手術後の手術部位感染や創傷治癒遅延を増加させない kn-title=The use of biologic disease-modifying antirheumatic drugs does not increase surgical site infection or delayed wound healing after orthopaedic surgeries for rheumatoid arthritis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KISOYohei en-aut-sei=KISO en-aut-mei=Yohei kn-aut-name=木曽洋平 kn-aut-sei=木曽 kn-aut-mei=洋平 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=４種類の醸造用麹菌による４種類の米の固体培養：ポリフェノール類とタンパク質の生物変換 kn-title=Aspergillus Solid-State Cultivation of Four Different Industrial Strains of Koji Mold on Four Different Rice Cultivars: Microbial Biotransformation of Phenolic Compounds and Proteins en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=JITPAKDEE JIRAYU en-aut-sei=JITPAKDEE JIRAYU en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=リボソーム・トランスロコン複合体の再局在化が、真核生物発生における小胞体出現の重要なイベントである証拠 kn-title=Evidence for the relocalization of the ribosome-translocon complex as a key event for the emergence of endoplasmic reticulum during eukaryogenesis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=CARILOIsaac en-aut-sei=CARILO en-aut-mei=Isaac kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama university kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=10 article-no= start-page=1164 end-page=1171 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240716 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cytosolic acidification and oxidation are the toxic mechanisms of SO2 in Arabidopsis guard cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=SO2/H2SO3 can damage plants. However, its toxic mechanism has still been controversial. Two models have been proposed, cytosolic acidification model and cellular oxidation model. Here, we assessed the toxic mechanism of H2SO3 in three cell types of Arabidopsis thaliana, mesophyll cells, guard cells (GCs), and petal cells. The sensitivity of GCs of Chloride channel a (CLCa)-knockout mutants to H2SO3 was significantly lower than those of wildtype plants. Expression of other CLC genes in mesophyll cells and petal cells were different from GCs. Treatment with antioxidant, disodium 4,5-dihydroxy-1,3-benzenedisulfonate (tiron), increased the median lethal concentration (LC50) of H2SO3 in GCs indicating the involvement of cellular oxidation, while the effect was negligible in mesophyll cells and petal cells. These results indicate that there are two toxic mechanisms of SO2 to Arabidopsis cells: cytosolic acidification and cellular oxidation, and the toxic mechanism may vary among cell types. en-copyright= kn-copyright= en-aut-name=MozhganiMahdi en-aut-sei=Mozhgani en-aut-mei=Mahdi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OoiLia en-aut-sei=Ooi en-aut-mei=Lia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=EspagneChristelle en-aut-sei=Espagne en-aut-mei=Christelle kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FilleurSophie en-aut-sei=Filleur en-aut-mei=Sophie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriIzumi C en-aut-sei=Mori en-aut-mei=Izumi C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC) kn-affil= affil-num=4 en-affil=Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC) kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=cytosolic acidification kn-keyword=cytosolic acidification en-keyword=Arabidopsis kn-keyword=Arabidopsis en-keyword=cellular oxidation kn-keyword=cellular oxidation en-keyword=chloride channel a kn-keyword=chloride channel a en-keyword=sulfur dioxide kn-keyword=sulfur dioxide END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=外来種アメリカザリガニの生態に関する研究：繁殖生物学および在来水生動物への影響 kn-title=Study on ecology of the invasive crayfish Procambarus clarkii in Japan: reproductive biology and the negative effects on native aquatic animal species en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=LUONG QUANG TUONG en-aut-sei=LUONG QUANG TUONG en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=サステナビリティのための生物学教育～理科教員養成におけるモジュール開発～ kn-title=Sustainability-Oriented Biology Education: Development of Science Teacher Training Modules en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KHALIFATULLOH FIEL ARDH en-aut-sei=KHALIFATULLOH FIEL ARDH en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Humanities and Social Sciences, Okayama University kn-affil=岡山大学大学院社会文化科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=鉄添加した底質微生物燃料電池を用いた農業排水路におけるリン内部負荷の削減 kn-title=Reduction of internal phosphorus loading in agricultural drainages using iron-incorporated sediment microbial fuel cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=GAMAMADA LIYANAGE ERANDI PRIYANGIKA PERERA en-aut-sei=GAMAMADA LIYANAGE ERANDI PRIYANGIKA PERERA en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=地球生物圏の過去と未来を理解するための、遷移元素の潜在的役割に関する研究 kn-title=Exploring the potential role of transitional elements to understand the past and the future of the Earth’s biosphere en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=RATNAYAKE MUDIYANSELAGE DILAN MADUSANKA RATNAYAKE en-aut-sei=RATNAYAKE MUDIYANSELAGE DILAN MADUSANKA RATNAYAKE en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama university kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=11 article-no= start-page=1323 end-page=1331 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The effect of exogenous dihydroxyacetone and methylglyoxal on growth, anthocyanin accumulation, and the glyoxalase system in Arabidopsis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Dihydroxyacetone (DHA) occurs in wide-ranging organisms, including plants, and can undergo spontaneous conversion to methylglyoxal (MG). While the toxicity of MG to plants is well-known, the toxicity of DHA to plants remains to be elucidated. We investigated the effects of DHA and MG on Arabidopsis. Exogenous DHA at up to 10 mM did not affect the radicle emergence, the expansion of green cotyledons, the seedling growth, or the activity of glyoxalase II, while DHA at 10 mM inhibited the root elongation and increased the activity of glyoxalase I. Exogenous MG at 1.0 mM inhibited these physiological responses and increased both activities. Dihydroxyacetone at 10 mM increased the MG content in the roots. These results indicate that DHA is not so toxic as MG in Arabidopsis seeds and seedlings and suggest that the toxic effect of DHA at high concentrations is attributed to MG accumulation by the conversion to MG. en-copyright= kn-copyright= en-aut-name=ZhaoMaoxiang en-aut-sei=Zhao en-aut-mei=Maoxiang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraToshiyuki en-aut-sei=Nakamura en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraYoshimasa en-aut-sei=Nakamura en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MunemasaShintaro en-aut-sei=Munemasa en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriIzumi C en-aut-sei=Mori en-aut-mei=Izumi C kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurataYoshiyuki en-aut-sei=Murata en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=dihydroxyacetone kn-keyword=dihydroxyacetone en-keyword=methylglyoxal kn-keyword=methylglyoxal en-keyword=growth kn-keyword=growth en-keyword=anthocyanin kn-keyword=anthocyanin en-keyword=glyoxalase system kn-keyword=glyoxalase system END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150724 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Newsletter from Course for Prospective Museum Workers, Faculty of Letters, Okayama University kn-title=学芸員課程 Newsletter 第5号 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=光本順 kn-aut-sei=光本 kn-aut-mei=順 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=洞出文美 kn-aut-sei=洞出 kn-aut-mei=文美 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=能勢基希 kn-aut-sei=能勢 kn-aut-mei=基希 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=脇田和樹 kn-aut-sei=脇田 kn-aut-mei=和樹 aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=鳥津亮二 kn-aut-sei=鳥津 kn-aut-mei=亮二 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil=文学部言語科学専修コース affil-num=3 en-affil= kn-affil=理学部生物学科 affil-num=4 en-affil= kn-affil=文学部言語科学専修コース affil-num=5 en-affil= kn-affil=八代市立博物館未来の森ミュージアム END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Newsletter from Course for Prospective Museum Workers, Faculty of Letters, Okayama University kn-title=学芸員課程 Newsletter 第3号 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=新納泉 kn-aut-sei=新納 kn-aut-mei=泉 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=光本順 kn-aut-sei=光本 kn-aut-mei=順 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=廣岡あすか kn-aut-sei=廣岡 kn-aut-mei=あすか aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=知光星香 kn-aut-sei=知光 kn-aut-mei=星香 aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岸川陽平 kn-aut-sei=岸川 kn-aut-mei=陽平 aut-affil-num=5 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=福冨幸 kn-aut-sei=福冨 kn-aut-mei=幸 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=文学部学芸員課程専門委員会 affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil=理学部生物学科 affil-num=4 en-affil= kn-affil=文学部　行動科学専修コース affil-num=5 en-affil= kn-affil=文学部　歴史文化学専修コース affil-num=6 en-affil= kn-affil=岡山県立美術館 END start-ver=1.4 cd-journal=joma no-vol=40 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20240321 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=鹿田遺跡 17 ―第22次調査地点―（地域医療人育成センターおかやま新営に伴う発掘調査） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=能城修一 kn-aut-sei=能城 kn-aut-mei=修一 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=木村理 kn-aut-sei=木村 kn-aut-mei=理 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=沖陽子 kn-aut-sei=沖 kn-aut-mei=陽子 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=㈱吉田生物研究所 kn-aut-sei=㈱吉田生物研究所 kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岩﨑志保 kn-aut-sei=岩﨑 kn-aut-mei=志保 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=明治大学黒耀石研究センター affil-num=2 en-affil= kn-affil=岡山大学文明動態学研究所 affil-num=3 en-affil= kn-affil=岡山県立大学 affil-num=4 en-affil= kn-affil= affil-num=5 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=135 cd-vols= no-issue=3 article-no= start-page=181 end-page=182 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20231201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The 95th Annual Meeting of the Japanese Tissue Culture Association kn-title=日本組織培養学会第95回大会報告 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaguchiMasakiyo en-aut-sei=Sakaguchi en-aut-mei=Masakiyo kn-aut-name=阪口政清 kn-aut-sei=阪口 kn-aut-mei=政清 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Cell Biology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域　細胞生物学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=好熱性真正細菌Bellilinea sp.由来の微生物型ロドプシンBeNaRにおける分子機能・特性解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KURIHARAMarie en-aut-sei=KURIHARA en-aut-mei=Marie kn-aut-name=栗原眞理恵 kn-aut-sei=栗原 kn-aut-mei=眞理恵 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol=291 cd-vols= no-issue=6 article-no= start-page=1119 end-page=1130 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20231020 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Hepatitis C virus NS5B triggers an MDA5-mediated innate immune response by producing dsRNA without the replication of viral genomes en-subtitle= kn-subtitle= en-abstract= kn-abstract=During the replication of viral genomes, RNA viruses produce double-stranded RNA (dsRNA), through the activity of their RNA-dependent RNA polymerases (RdRps) as viral replication intermediates. Recognition of viral dsRNA by host pattern recognition receptors – such as retinoic acid-induced gene-I (RIG-I)-like receptors and Toll-like receptor 3 – triggers the production of interferon (IFN)-β via the activation of IFN regulatory factor (IRF)-3. It has been proposed that, during the replication of viral genomes, each of RIG-I and melanoma differentiation-associated gene 5 (MDA5) form homodimers for the efficient activation of a downstream signalling pathway in host cells. We previously reported that, in the non-neoplastic human hepatocyte line PH5CH8, the RdRp NS5B derived from hepatitis C virus (HCV) could induce IFN-β expression by its RdRp activity without the actual replication of viral genomes. However, the exact mechanism by which HCV NS5B produced IFN-β remained unknown. In the present study, we first showed that NS5B derived from another Flaviviridae family member, GB virus B (GBV-B), also possessed the ability to induce IFN-β in PH5CH8 cells. Similarly, HCV NS5B, but not its G317V mutant, which lacks RdRp activity, induced the dimerization of MDA5 and subsequently the activation of IRF-3. Interestingly, immunofluorescence analysis showed that HCV NS5B produced dsRNA. Like HCV NS5B, GBV-B NS5B also triggered the production of dsRNA and subsequently the dimerization of MDA5. Taken together, our results show that HCV NS5B triggers an MDA5-mediated innate immune response by producing dsRNA without the replication of viral genomes in human hepatocytes. en-copyright= kn-copyright= en-aut-name=DansakoHiromichi en-aut-sei=Dansako en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AriumiYasuo en-aut-sei=Ariumi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TogashiYosuke en-aut-sei=Togashi en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Division of Biological Information Technology, Joint Research Center for Human Retrovirus Infection, Kagoshima University kn-affil= affil-num=3 en-affil=Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases kn-affil= affil-num=4 en-affil=Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Department of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=double-stranded RNA kn-keyword=double-stranded RNA en-keyword=hepatitis C virus kn-keyword=hepatitis C virus en-keyword=innate immunity kn-keyword=innate immunity en-keyword=RIG-I-like receptor kn-keyword=RIG-I-like receptor en-keyword=RNA virus kn-keyword=RNA virus END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=三環性融合ピリミジン類の合成と生物活性分子設計 kn-title=Synthesis and biological active molecular design of tricyclic fused pyrimidines en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Shoeb Ahmed en-aut-sei=Shoeb Ahmed en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue= article-no= start-page=46 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ancient DNA studies of human remains kn-title=生物遺骸に残された“DNA の記録” を読み解く en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MIZUNOFuzuki en-aut-sei=MIZUNO en-aut-mei=Fuzuki kn-aut-name=水野文月 kn-aut-sei=水野 kn-aut-mei=文月 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=東邦大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220922 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=じん肺患者における悪性新生物の標準化罹患比 kn-title=Standardized incidence ratios of malignant neoplasms among patients with pneumoconiosis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MICHIBATATatsuya en-aut-sei=MICHIBATA en-aut-mei=Tatsuya kn-aut-name=道端達也 kn-aut-sei=道端 kn-aut-mei=達也 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol=39 cd-vols= no-issue=5 article-no= start-page=459 end-page=467 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Constant Light, Pdp1, and Tim Exert Influence on Free-Running Period of Locomotor Rhythms in the Cricket Gryllus bimaculatus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Most insects show circadian rhythms of which the free-running period changes in a light-dependent manner and is generally longer under constant light (LL) than under constant dark conditions in nocturnal animals. However, the mechanism underlying this LL-dependent period change remains unclear. Here, using the cricket Gryllus bimaculatus, we examined the effects of long-term LL exposure on the free-running period of locomotor rhythms. Initially, the free-running period was considerably longer than 24 h but it gradually became shorter during long-term exposure to LL. The initiallengthening and ensuing gradual shortening under long-term LL exposure were observed evenafter unilateral removal of the optic lobe. Thus, these changes in the free-running period could be attributable to a single optic lobe clock. RNA interference (RNAi)-mediated silencing of the clock genes Par domain protein 1 (Pdp1) and timeless (tim) revealed that the treatments eliminated the initial period lengthening by LL without reducing circadian photoreceptor gene expression. However, they did not affect the period shortening during long-term LL exposure. The slopes of the regression line for the period change during long-term LL for Pdp1RNAi-treated and timRNAi-treated crickets were not different from that of the dsDsRed2-treated control. These results suggest that the initial period lengthening after transfer to LL requires tim and Pdp1, while the ensuing period shortening during long-term LL exposure is caused by a mechanism independent of tim and Pdp1. en-copyright= kn-copyright= en-aut-name=MoriyamaYoshiyuki en-aut-sei=Moriyama en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeuchiKazuki en-aut-sei=Takeuchi en-aut-mei=Kazuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TomiokaKenji en-aut-sei=Tomioka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Natural Sciences, Kawasaki Medical School, Kurashiki 701-0192, Japan kn-affil= affil-num=2 en-affil=Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan kn-affil= affil-num=3 en-affil=Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan kn-affil= en-keyword=circadian rhythm kn-keyword=circadian rhythm en-keyword=cricket kn-keyword=cricket en-keyword=free-running period kn-keyword=free-running period en-keyword=constant light kn-keyword=constant light en-keyword=clock gene kn-keyword=clock gene END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue= article-no= start-page=921635 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220707 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Siderophore for Lanthanide and Iron Uptake for Methylotrophy and Plant Growth Promotion in Methylobacterium aquaticum Strain 22A en-subtitle= kn-subtitle= en-abstract= kn-abstract=Methylobacterium and Methylorubrum species are facultative methylotrophic bacteria that are abundant in the plant phyllosphere. They have two methanol dehydrogenases, MxaF and XoxF, which are dependent on either calcium or lanthanides (Lns), respectively. Lns exist as insoluble minerals in nature, and their solubilization and uptake require a siderophore-like substance (lanthanophore). Methylobacterium species have also been identified as plant growth-promoting bacteria although the actual mechanism has not been well-investigated. This study aimed to reveal the roles of siderophore in Methylobacterium aquaticum strain 22A in Ln uptake, bacterial physiology, and plant growth promotion. The strain 22A genome contains an eight-gene cluster encoding the staphyloferrin B-like (sbn) siderophore. We demonstrate that the sbn siderophore gene cluster is necessary for growth under low iron conditions and was complemented by supplementation with citrate or spent medium of the wild type or other strains of the genera. The siderophore exhibited adaptive features, including tolerance to oxidative and nitrosative stress, biofilm formation, and heavy metal sequestration. The contribution of the siderophore to plant growth was shown by the repressive growth of duckweed treated with siderophore mutant under iron-limited conditions; however, the siderophore was dispensable for strain 22A to colonize the phyllosphere. Importantly, the siderophore mutant could not grow on methanol, but the siderophore could solubilize insoluble Ln oxide, suggesting its critical role in methylotrophy. We also identified TonB-dependent receptors (TBDRs) for the siderophore-iron complex, iron citrate, and Ln, among 12 TBDRs in strain 22A. Analysis of the siderophore synthesis gene clusters and TBDR genes in Methylobacterium genomes revealed the existence of diverse types of siderophores and TBDRs. Methylorubrum species have an exclusive TBDR for Ln uptake that has been identified as LutH. Collectively, the results of this study provide insight into the importance of the sbn siderophore in Ln chelation, bacterial physiology, and the diversity of siderophore and TBDRs in Methylobacterium species. en-copyright= kn-copyright= en-aut-name=JumaPatrick Otieno en-aut-sei=Juma en-aut-mei=Patrick Otieno kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=FujitaniYoshiko en-aut-sei=Fujitani en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AlessaOla en-aut-sei=Alessa en-aut-mei=Ola kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OyamaTokitaka en-aut-sei=Oyama en-aut-mei=Tokitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=YurimotoHiroya en-aut-sei=Yurimoto en-aut-mei=Hiroya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SakaiYasuyoshi en-aut-sei=Sakai en-aut-mei=Yasuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Science, Kyoto University kn-affil= affil-num=5 en-affil=Graduate School of Agriculture, Kyoto University kn-affil= affil-num=6 en-affil=Graduate School of Agriculture, Kyoto University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=Methylobacterium species kn-keyword=Methylobacterium species en-keyword=lanthanide kn-keyword=lanthanide en-keyword=lanthanophore kn-keyword=lanthanophore en-keyword=siderophore kn-keyword=siderophore en-keyword=plant growth promoter kn-keyword=plant growth promoter en-keyword=heavy metal sequestration kn-keyword=heavy metal sequestration END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue= article-no= start-page=921636 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220623 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Periplasmic Lanthanide Mediator, Lanmodulin, in Methylobacterium aquaticum Strain 22A en-subtitle= kn-subtitle= en-abstract= kn-abstract=Methylobacterium and Methylorubrum species oxidize methanol via pyrroloquinoline quinone-methanol dehydrogenases (MDHs). MDHs can be classified into two major groups, Ca2+-dependent MDH (MxaF) and lanthanide (Ln(3+))-dependent MDH (XoxF), whose expression is regulated by the availability of Ln(3+). A set of a siderophore, TonB-dependent receptor, and an ABC transporter that resembles the machinery for iron uptake is involved in the solubilization and transport of Ln(3+). The transport of Ln(3+) into the cytosol enhances XoxF expression. A unique protein named lanmodulin from Methylorubrum extorquens strain AM1 was identified as a specific Ln(3+)-binding protein, and its biological function was implicated to be an Ln(3+) shuttle in the periplasm. In contrast, it remains unclear how Ln(3+) levels in the cells are maintained, because Ln(3+) is potentially deleterious to cellular systems due to its strong affinity to phosphate ions. In this study, we investigated the function of a lanmodulin homolog in Methylobacterium aquaticum strain 22A. The expression of a gene encoding lanmodulin (lanM) was induced in response to the presence of La3+. A recombinant LanM underwent conformational change upon La3+ binding. Phenotypic analyses on lanM deletion mutant and overexpressing strains showed that LanM is not necessary for the wild-type and XoxF-dependent mutant's methylotrophic growth. We found that lanM expression was regulated by MxcQE (a two-component regulator for MxaF) and TonB_Ln (a TonB-dependent receptor for Ln(3+)). The expression level of mxcQE was altered to be negatively dependent on Ln(3+) concentration in increment lanM, whereas it was constant in the wild type. Furthermore, when exposed to La3+, increment lanM showed an aggregating phenotype, cell membrane impairment, La deposition in the periplasm evidenced by electron microscopy, differential expression of proteins involved in membrane integrity and phosphate starvation, and possibly lower La content in the membrane vesicle (MV) fractions. Taken together, we concluded that lanmodulin is involved in the complex regulation mechanism of MDHs and homeostasis of cellular Ln levels by facilitating transport and MV-mediated excretion. en-copyright= kn-copyright= en-aut-name=FujitaniYoshiko en-aut-sei=Fujitani en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShibataTakeshi en-aut-sei=Shibata en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=K.K. AB SCIEX kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=lanmodulin kn-keyword=lanmodulin en-keyword=lanthanide kn-keyword=lanthanide en-keyword=methanol dehydrogenase kn-keyword=methanol dehydrogenase en-keyword=Methylobacterium species kn-keyword=Methylobacterium species en-keyword=membrane vesicles kn-keyword=membrane vesicles END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=真核生物タンパク質装置のアーキア起源の探索 kn-title=Exploring the archaeal origin of eukaryotic protein machineries en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TRAN THUY LINH en-aut-sei=TRAN THUY LINH en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama university kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220328 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=鹿田遺跡16　― 第20次調査Ｂ・Ｄ地点 ―（岡山大学病院中央診療棟新営に伴う発掘調査） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IwasakiShiho en-aut-sei=Iwasaki en-aut-mei=Shiho kn-aut-name=岩﨑志保 kn-aut-sei=岩﨑 kn-aut-mei=志保 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=能城修一 kn-aut-sei=能城 kn-aut-mei=修一 aut-affil-num=2 ORCID= en-aut-name=OkiYoko en-aut-sei=Oki en-aut-mei=Yoko kn-aut-name=沖陽子 kn-aut-sei=沖 kn-aut-mei=陽子 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=富岡直人 kn-aut-sei=富岡 kn-aut-mei=直人 aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=（株）吉田生物研究所 kn-aut-sei=（株）吉田生物研究所 kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=パレオ・ラボAMS年代測定グループ kn-aut-sei=パレオ・ラボAMS年代測定グループ kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=2 en-affil= kn-affil=明治大学黒燿石研究センター affil-num=3 en-affil= kn-affil=岡山県立大学 affil-num=4 en-affil= kn-affil=岡山理科大学 affil-num=5 en-affil= kn-affil= affil-num=6 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=179 cd-vols= no-issue= article-no= start-page=121 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220224 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Production of Aquatic Organism Cultivation Kit and Its Development in STEAM Education kn-title=水産生物栽培キットの設計・製作とSTEAM教育への展開 en-subtitle= kn-subtitle= en-abstract= kn-abstract=中学校技術科における水産生物の栽培では，実習的な活動はほとんど実施されていない。知識だけの習得で，思考を深めることは難しい。実社会での問題発見や解決を科目横断的に進めるSTEAM教育により，思考を抽象化させる必要があると考える。本研究では，はじめに「水産生物栽培キット」を設計・製作し，中学校技術科での教材としての適性を確かめた。次に，水産生物栽培キットをSTEAM教育へ展開するため，ファンバブル発生ノズルを水産生物栽培キットに設置し，ファインバブルがゼブラフィッシュの成長に及ぼす影響を調べた。その結果，水産生物栽培キットは中学校技術科の水産生物の栽培で，学習指導要領の取り扱い内容を満足する教材として使用できることを確認した。また，ファインバブルによるゼブラフィッシュの成長促進や高品質化に関する優位性は確認できなかったが，ものづくりに関するテーマは，STEAM教育に寄与する可能性が高いことを明らかにした。 en-copyright= kn-copyright= en-aut-name=NogeHirofumi en-aut-sei=Noge en-aut-mei=Hirofumi kn-aut-name=野毛宏文 kn-aut-sei=野毛 kn-aut-mei=宏文 aut-affil-num=1 ORCID= en-aut-name=KajimotoTatsuya en-aut-sei=Kajimoto en-aut-mei=Tatsuya kn-aut-name=梶元達也 kn-aut-sei=梶元 kn-aut-mei=達也 aut-affil-num=2 ORCID= en-aut-name=IrieTakashi en-aut-sei=Irie en-aut-mei=Takashi kn-aut-name=入江隆 kn-aut-sei=入江 kn-aut-mei=隆 aut-affil-num=3 ORCID= en-aut-name=KasaiToshinobu en-aut-sei=Kasai en-aut-mei=Toshinobu kn-aut-name=笠井俊信 kn-aut-sei=笠井 kn-aut-mei=俊信 aut-affil-num=4 ORCID= en-aut-name=NitoKenji en-aut-sei=Nito en-aut-mei=Kenji kn-aut-name=内藤憲二 kn-aut-sei=内藤 kn-aut-mei=憲二 aut-affil-num=5 ORCID= en-aut-name=HirataSeiji en-aut-sei=Hirata en-aut-mei=Seiji kn-aut-name=平田晴路 kn-aut-sei=平田 kn-aut-mei=晴路 aut-affil-num=6 ORCID= affil-num=1 en-affil=Division of Life, Health and Sports Education, Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科　生活・健康スポーツ系 affil-num=2 en-affil=Okayama City Sonan Public Hall kn-affil=岡山市操南公民館 affil-num=3 en-affil=Division of Life, Health and Sports Education, Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科　生活・健康スポーツ系 affil-num=4 en-affil=Division of Life, Health and Sports Education, Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科　生活・健康スポーツ系 affil-num=5 en-affil=Division of Life, Health and Sports Education, Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科　生活・健康スポーツ系 affil-num=6 en-affil=Division of Life, Health and Sports Education, Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科　生活・健康スポーツ系 en-keyword=水産生物の栽培 kn-keyword=水産生物の栽培 en-keyword=水産生物栽培キット kn-keyword=水産生物栽培キット en-keyword=STEAM教育 kn-keyword=STEAM教育 en-keyword=ゼブラフィッシュ kn-keyword=ゼブラフィッシュ en-keyword=ファインバブル kn-keyword=ファインバブル END start-ver=1.4 cd-journal=joma no-vol=133 cd-vols= no-issue=3 article-no= start-page=158 end-page=165 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20211201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Understanding the mesenchymal stem cell and its application to the study of human pluripotent stem cells kn-title=間葉系幹細胞の細胞生物学的特徴づけと，ヒト多能性幹細胞研究への応用 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakaradaTakeshi en-aut-sei=Takarada en-aut-mei=Takeshi kn-aut-name=宝田剛志 kn-aut-sei=宝田 kn-aut-mei=剛志 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学学術研究院医歯薬学域　組織機能修復学 en-keyword=間葉系幹細胞 kn-keyword=間葉系幹細胞 en-keyword=ヒト多能性幹細胞 kn-keyword=ヒト多能性幹細胞 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210924 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=自己抗体バイオマーカー解析のための組換えがん精巣抗原の生物物理学的特性に関する研究) kn-title=Study on the biophysical property of recombinant cancer-testis antigens for autoantibody biomarker analysis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AHMADIGHADYKOLAEI Hannaneh en-aut-sei=AHMADIGHADYKOLAEI Hannaneh en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University kn-affil=岡山大学大学院ヘルスシステム統合科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210924 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=腫瘍内18F-fluorodeoxyglucose 最大取込み細胞の細胞生物学的特性に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SugitaSachi en-aut-sei=Sugita en-aut-mei=Sachi kn-aut-name=杉田幸 kn-aut-sei=杉田 kn-aut-mei=幸 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210924 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=多様な菌類および植物パルティティウイルスの分子生物学的・生物学的性状 kn-title=Molecular and biological properties of diverse fungal and plant partitiviruses en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TELENGECH Paul Kipkemboi en-aut-sei=TELENGECH Paul Kipkemboi en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210924 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=生物学的に活性な抗酸化物質の豊富な供給源としての米エタノール抽出物の評価 kn-title=Evaluation and classification of rice ethanol extracts as a rich source of biologically-active antioxidants en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WuHongyan en-aut-sei=Wu en-aut-mei=Hongyan kn-aut-name=呉虹燕 kn-aut-sei=呉 kn-aut-mei=虹燕 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=740610 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20211006 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comprehensive Comparative Genomics and Phenotyping of Methylobacterium Species en-subtitle= kn-subtitle= en-abstract= kn-abstract=The pink-pigmented facultative methylotrophs (PPFMs), a major bacterial group found in the plant phyllosphere, comprise two genera: Methylobacterium and Methylorubrum. They have been separated into three major clades: A, B (Methylorubrum), and C. Within these genera, however, some species lack either pigmentation or methylotrophy, which raises the question of what actually defines the PPFMs. The present study employed a comprehensive comparative genomics approach to reveal the phylogenetic relationship among the PPFMs and to explain the genotypic differences that confer their different phenotypes. We newly sequenced the genomes of 29 relevant-type strains to complete a dataset for almost all validly published species in the genera. Through comparative analysis, we revealed that methylotrophy, nitrate utilization, and anoxygenic photosynthesis are hallmarks differentiating the PPFMs from the other Methylobacteriaceae. The Methylobacterium species in clade A, including the type species Methylobacterium organophilum, were phylogenetically classified into six subclades, each possessing relatively high genomic homology and shared phenotypic characteristics. One of these subclades is phylogenetically close to Methylorubrum species; this finding led us to reunite the two genera into a single genus Methylobacterium. Clade C, meanwhile, is composed of phylogenetically distinct species that share relatively higher percent G+C content and larger genome sizes, including larger numbers of secondary metabolite clusters. Most species of clade C and some of clade A have the glutathione-dependent pathway for formaldehyde oxidation in addition to the H4MPT pathway. Some species cannot utilize methanol due to their lack of MxaF-type methanol dehydrogenase (MDH), but most harbor an XoxF-type MDH that enables growth on methanol in the presence of lanthanum. The genomes of PPFMs encode between two and seven (average 3.7) genes for pyrroloquinoline quinone-dependent alcohol dehydrogenases, and their phylogeny is distinctly correlated with their genomic phylogeny. All PPFMs were capable of synthesizing auxin and did not induce any immune response in rice cells. Other phenotypes including sugar utilization, antibiotic resistance, and antifungal activity correlated with their phylogenetic relationship. This study provides the first inclusive genotypic insight into the phylogeny and phenotypes of PPFMs. en-copyright= kn-copyright= en-aut-name=AlessaOla en-aut-sei=Alessa en-aut-mei=Ola kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OguraYoshitoshi en-aut-sei=Ogura en-aut-mei=Yoshitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujitaniYoshiko en-aut-sei=Fujitani en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakamiHideto en-aut-sei=Takami en-aut-mei=Hideto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HayashiTetsuya en-aut-sei=Hayashi en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SahinNurettin en-aut-sei=Sahin en-aut-mei=Nurettin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Division of Microbiology, Department of Infectious Medicine, Kurume University School of Medicine kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Atmosphere and Ocean Research Institute, The University of Tokyo kn-affil= affil-num=5 en-affil=Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University kn-affil= affil-num=6 en-affil=Egitim Fakultesi, Mugla Sitki Kocman University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=Methylobacterium kn-keyword=Methylobacterium en-keyword=comparative genomics kn-keyword=comparative genomics en-keyword=methylotroph kn-keyword=methylotroph en-keyword=methanol dehydrogenase kn-keyword=methanol dehydrogenase en-keyword=Methylorubrum kn-keyword=Methylorubrum END start-ver=1.4 cd-journal=joma no-vol=141 cd-vols= no-issue=10 article-no= start-page=1155 end-page=1160 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=2021101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Biophysical and Biochemical Research of Animal Rhodopsins kn-title=光受容タンパク質・ロドプシンの生物物理化学研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Opsins (also called animal rhodopsins) are universal photoreceptive proteins that provide the molecular basis of visual and nonvisual photoreception in animals, including humans. Opsins consist of seven helical a-transmembrane domains and use retinal, a derivative of vitamin A, as a chromophore. In many opsins, light absorption triggers photoisomerization from 11-cis retinal to all-trans retinal, resulting in activation via dynamic structural changes in the protein moiety. Activated opsins stimulate cognate trimeric G proteins to induce signal transduction cascades in cells. Recently, molecular and physiological analyses of diverse opsins have progressively advanced. This review introduces the molecular basis and physiological unctions of opsins. Based on the functions of opsins, I will discuss the potential of opsins as target molecules to treat and prevent visual and nonvisual diseases such as sleep disorder and depression. en-copyright= kn-copyright= en-aut-name=KojimaKeiichi en-aut-sei=Kojima en-aut-mei=Keiichi kn-aut-name=小島慧一 kn-aut-sei=小島 kn-aut-mei=慧一 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学学術研究院医歯薬学域 en-keyword=rhodopsin kn-keyword=rhodopsin en-keyword=opsin kn-keyword=opsin en-keyword=retinal kn-keyword=retinal en-keyword=G protein coupled receptor kn-keyword=G protein coupled receptor en-keyword=vision kn-keyword=vision END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=715545 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210819 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat en-subtitle= kn-subtitle= en-abstract= kn-abstract=Yellow mosaic disease in winter wheat is usually attributed to the infection by bymoviruses or furoviruses; however, there is still limited information on whether other viral agents are also associated with this disease. To investigate the wheat viromes associated with yellow mosaic disease, we carried out de novo RNA sequencing (RNA-seq) analyses of symptomatic and asymptomatic wheat-leaf samples obtained from a field in Hokkaido, Japan, in 2018 and 2019. The analyses revealed the infection by a novel betaflexivirus, which tentatively named wheat virus Q (WVQ), together with wheat yellow mosaic virus (WYMV, a bymovirus) and northern cereal mosaic virus (a cytorhabdovirus). Basic local alignment search tool (BLAST) analyses showed that the WVQ strains (of which there are at least three) were related to the members of the genus Foveavirus in the subfamily Quinvirinae (family Betaflexiviridae). In the phylogenetic tree, they form a clade distant from that of the foveaviruses, suggesting that WVQ is a member of a novel genus in the Quinvirinae. Laboratory tests confirmed that WVQ, like WYMV, is potentially transmitted through the soil to wheat plants. WVQ was also found to infect rye plants grown in the same field. Moreover, WVQ-derived small interfering RNAs accumulated in the infected wheat plants, indicating that WVQ infection induces antiviral RNA silencing responses. Given its common coexistence with WYMV, the impact of WVQ infection on yellow mosaic disease in the field warrants detailed investigation. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YoshidaNaoto en-aut-sei=Yoshida en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FujitaMiki en-aut-sei=Fujita en-aut-mei=Miki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MaruyamaKazuyuki en-aut-sei=Maruyama en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HyodoKiwamu en-aut-sei=Hyodo en-aut-mei=Kiwamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HisanoHiroshi en-aut-sei=Hisano en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TamadaTetsuo en-aut-sei=Tamada en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AndikaIda Bagus en-aut-sei=Andika en-aut-mei=Ida Bagus kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SuzukiNobuhiro en-aut-sei=Suzuki en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=2 en-affil=Agricultural Research Institute, HOKUREN Federation of Agricultural Cooperatives kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=8 en-affil=College of Plant Health and Medicine, Qingdao Agricultural University kn-affil= affil-num=9 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= en-keyword=Betaflexiviridae kn-keyword=Betaflexiviridae en-keyword=quinvirus kn-keyword=quinvirus en-keyword=bymovirus kn-keyword=bymovirus en-keyword=yellow mosaic disease kn-keyword=yellow mosaic disease en-keyword=wheat kn-keyword=wheat en-keyword=virome kn-keyword=virome en-keyword=soil borne kn-keyword=soil borne en-keyword=variants kn-keyword=variants END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=原生生物タイヨウチュウRaphidocystis contractilisの急速な軸足収縮機構に関する研究 kn-title=Studies on mechanisms of rapid axopodial contraction in the centrohelid Raphidocystis contractilis en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IkedaRisa en-aut-sei=Ikeda en-aut-mei=Risa kn-aut-name=池田理佐 kn-aut-sei=池田 kn-aut-mei=理佐 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=食品及び醸造副産物の微生物発酵による品質保持に関する研究 kn-title=Microbial fermentation and quality preservation of agro-industrial wet by-products en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WaliAjmal en-aut-sei=Wali en-aut-mei=Ajmal kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=菌類ウイルスの植物病原糸状菌への導入と生物学的性状解析 kn-title=Experimental introduction of mycoviruses into phytopathogenic filamentous fungi and their biologic characterization en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SabitreeShahi en-aut-sei=Sabitree en-aut-mei=Shahi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=C-配糖体mangiferinのアグリコンnorathyriolへの微生物変換: 活性菌株の分布，単離と諸性質検討 kn-title=Microbial conversion of C-glycoside mangiferin into its aglycone norathyriol: Distribution, isolation, and characterization of active bacteria en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=UswatunHasanah en-aut-sei=Uswatun en-aut-mei=Hasanah kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol=132 cd-vols= no-issue=3 article-no= start-page=131 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20201201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hepatitis C virus (HCV) : Development of anti-HCV agents and anti-HCV therapy kn-title=Ｃ型肝炎ウイルス（HCV）：抗 HCV 剤の開発と抗 HCV 療法 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name=加藤宣之 kn-aut-sei=加藤 kn-aut-mei=宣之 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　腫瘍ウイルス学 en-keyword=インターフェロン kn-keyword=インターフェロン en-keyword=リバビリン kn-keyword=リバビリン en-keyword=HCVレプリコンシステム kn-keyword=HCVレプリコンシステム en-keyword=抗HCVアッセイシステム kn-keyword=抗HCVアッセイシステム en-keyword= DAA kn-keyword= DAA END start-ver=1.4 cd-journal=joma no-vol=2019 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210319 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Bulletin of Archaeological Research Center Okayama University 2019 kn-title=岡山大学埋蔵文化財調査研究センター紀要2019 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MinamiKentaro en-aut-sei=Minami en-aut-mei=Kentaro kn-aut-name=南健太郎 kn-aut-sei=南 kn-aut-mei=健太郎 aut-affil-num=1 ORCID= en-aut-name=YamaguchiYuji en-aut-sei=Yamaguchi en-aut-mei=Yuji kn-aut-name=山口雄治 kn-aut-sei=山口 kn-aut-mei=雄治 aut-affil-num=2 ORCID= en-aut-name=NozakiTakahiro en-aut-sei=Nozaki en-aut-mei=Takahiro kn-aut-name=野崎貴博 kn-aut-sei=野崎 kn-aut-mei=貴博 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=宇田津徹朗 kn-aut-sei=宇田津 kn-aut-mei=徹朗 aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=田崎博之 kn-aut-sei=田崎 kn-aut-mei=博之 aut-affil-num=5 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=吉田生物研究所 kn-aut-sei=吉田生物研究所 kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IwasakiShiho en-aut-sei=Iwasaki en-aut-mei=Shiho kn-aut-name=岩﨑志保 kn-aut-sei=岩﨑 kn-aut-mei=志保 aut-affil-num=7 ORCID= affil-num=1 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=2 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=3 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=4 en-affil= kn-affil=宮崎大学 affil-num=5 en-affil= kn-affil=愛媛大学 affil-num=6 en-affil= kn-affil= affil-num=7 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page=149 end-page=163 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210322 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An Interdisciplinary Approach for ESD-oriented Understanding of Natural Environmental Systems through Collaboration between Meteorology and Botany: Practical Trials in University Classes kn-title=気象学と植物学との連携による自然環境系の ESD 的理解への学際的アプローチ―大学における授業実践の試み― en-subtitle= kn-subtitle= en-abstract=Practical lessons for liberal arts in university aimed at education for sustainable development (ESD)-oriented understanding of the natural environmental systems were conducted through collaboration between meteorological and botanical scientific studies. As the first trial, some interdisciplinary themes were identified, based on subject contents in science education in which the relationship between weather/seasons/climate and plants is important. In particular, an analysis of the lesson, “Seasons and plants”, revealed that phenology was helpful in enhancing students’ understanding of the relationship between the seasonal cycle of weather/climate and plant growth and responses to the environment associated with ESD. These practical lessons proved that collaboration between meteorology and botany is valuable for both the promotion of ESD-oriented understanding of the natural environmental systems and cross-cutting consideration of the subject content in science education, providing a promising approach for teacher education. kn-abstract=理科の学問分野である気象学と植物学との連携により，持続可能な開発のための教育（ESD）を志向した自然環境系の理解を目指し，大学の教科・分野横断的な科目における授業実践を行った。最初の試みとして，気象・季節・気候および植物との関連が重要となる理科の教科内容をベースに，学際的な側面を持ついくつかのテーマが見出された。とりわけ，生物季節（フェノロジー）は，ESD に関連した気象・気候の季節サイクルと植物の成長および環境応答との関わりに関する学生の理解を促進するうえで有用であることが，授業分析から示唆された。これらの授業実践から，気象学と植物学との連携が，自然環境系の ESD 的理解の促進のみならず，教科内容構成の分野横断的な検討にも役立ち，教師教育のためのアプローチとして有望である可能性が示された。 en-copyright= kn-copyright= en-aut-name=HARADATaro en-aut-sei=HARADA en-aut-mei=Taro kn-aut-name=原田太郎 kn-aut-sei=原田 kn-aut-mei=太郎 aut-affil-num=1 ORCID= en-aut-name=KATOKuranoshin en-aut-sei=KATO en-aut-mei=Kuranoshin kn-aut-name=加藤内藏進 kn-aut-sei=加藤 kn-aut-mei=内藏進 aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科 affil-num=2 en-affil=Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科 en-keyword=ESD kn-keyword=ESD en-keyword=教科内容構成 (subject contents organization) kn-keyword=教科内容構成 (subject contents organization) en-keyword=東アジアの気候系 (climate system in East Asia) kn-keyword=東アジアの気候系 (climate system in East Asia) en-keyword=生物季節 (phenology) kn-keyword=生物季節 (phenology) en-keyword=園芸学 (horticulture) kn-keyword=園芸学 (horticulture) END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue=1 article-no= start-page=12 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20201111 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The role of clockwork orange in the circadian clock of the cricket Gryllus bimaculatus en-subtitle= kn-subtitle= en-abstract= kn-abstract=The circadian clock generates rhythms of approximately 24 h through periodic expression of the clock genes. In insects, the major clock genes period (per) and timeless (tim) are rhythmically expressed upon their transactivation by CLOCK/CYCLE, with peak levels in the early night. In Drosophila, clockwork orange (cwo) is known to inhibit the transcription of per and tim during the daytime to enhance the amplitude of the rhythm, but its function in other insects is largely unknown. In this study, we investigated the role of cwo in the clock mechanism of the cricket Gryllus bimaculatus. The results of quantitative RT-PCR showed that under a light/dark (LD) cycle, cwo is rhythmically expressed in the optic lobe (lamina-medulla complex) and peaks during the night. When cwo was knocked down via RNA interference (RNAi), some crickets lost their locomotor rhythm, while others maintained a rhythm but exhibited a longer free-running period under constant darkness (DD). In cwo(RNAi) crickets, all clock genes except for cryptochrome 2 (cry2) showed arrhythmic expression under DD; under LD, some of the clock genes showed higher mRNA levels, and tim showed rhythmic expression with a delayed phase. Based on these results, we propose that cwo plays an important role in the cricket circadian clock. en-copyright= kn-copyright= en-aut-name=TomiyamaYasuaki en-aut-sei=Tomiyama en-aut-mei=Yasuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShinoharaTsugumichi en-aut-sei=Shinohara en-aut-mei=Tsugumichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsukaMirai en-aut-sei=Matsuka en-aut-mei=Mirai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MitoTaro en-aut-sei=Mito en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TomiokaKenji en-aut-sei=Tomioka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Technology, Industrial and Social Sciences, Tokushima University kn-affil= affil-num=6 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil= en-keyword=Circadian clock kn-keyword=Circadian clock en-keyword=Clockwork orange kn-keyword=Clockwork orange en-keyword=Clock gene kn-keyword=Clock gene en-keyword=Cricket kn-keyword=Cricket en-keyword=cry2 kn-keyword=cry2 en-keyword=Molecular oscillation kn-keyword=Molecular oscillation en-keyword=Locomotor rhythm kn-keyword=Locomotor rhythm END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=イネの亜鉛吸収に関する生理学的及び分子生物学的解析 kn-title=Physiological and Molecular Characterization of Zinc Uptake in Rice en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HuangSheng en-aut-sei=Huang en-aut-mei=Sheng kn-aut-name=黄勝 kn-aut-sei=黄 kn-aut-mei=勝 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=生物活性天然物の立体発散的合成研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WadasHiroko en-aut-sei=Wadas en-aut-mei=Hiroko kn-aut-name=和田寛子 kn-aut-sei=和田 kn-aut-mei=寛子 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=132 cd-vols= no-issue=2 article-no= start-page=60 end-page=67 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200803 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hepatitis C virus (HCV)：Diversity and variation of RNA genome kn-title=C型肝炎ウイルス（HCV）：RNAゲノムの多様性と変異性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name=加藤宣之 kn-aut-sei=加藤 kn-aut-mei=宣之 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　腫瘍ウイルス学 en-keyword=HCV ゲノム kn-keyword=HCV ゲノム en-keyword=レプリコン複製細胞 kn-keyword=レプリコン複製細胞 en-keyword=長期継代培養 kn-keyword=長期継代培養 en-keyword=遺伝子解析 kn-keyword=遺伝子解析 en-keyword=準種 kn-keyword=準種 END start-ver=1.4 cd-journal=joma no-vol=30 cd-vols= no-issue=1-3 article-no= start-page=215 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=200305 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Orchid Fleck Virus: Brevipalpus californicus Mite Transmission, Biological Properties and Genome Structure en-subtitle= kn-subtitle= en-abstract= kn-abstract=Orchid fleck virus (OFV) causes necrotic or chlorotic ring spots and fleck symptoms in many orchid species world-wide. The virus has non-enveloped, bacilliform particles of about 40 nm × 100–150 nm and is sap-transmissible to several plant species. OFV is transmitted by the mite Brevipalpus californicus (Banks) in a persistent manner and efficiently transmitted by both adults and nymphs, but not by larvae. Viruliferous mites retain their infectivity for 3 weeks on a virus-immune host. The genome of OFV consists of two molecules of 6431 (RNA1) and 6001 nucleotides (RNA2). The RNAs have conserved and complementary terminal sequences. RNA1 contains five open reading frames (ORF), and RNA2 encodes a single ORF. Although some of the encoded proteins of OFV have sequences similar to those of proteins of plant rhabdoviruses, OFV differs from viruses in the family Rhabdoviridae in having a bipartite genome. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TamadaTetsuo en-aut-sei=Tamada en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Research Institute for Bioresources, Okayama University kn-affil= affil-num=2 en-affil=Research Institute for Bioresources, Okayama University kn-affil= affil-num=3 en-affil=Research Institute for Bioresources, Okayama University kn-affil= en-keyword=Brevipalpus californicus kn-keyword=Brevipalpus californicus en-keyword=mite transmission kn-keyword=mite transmission en-keyword=orchids kn-keyword=orchids en-keyword=orchid fleck virus kn-keyword=orchid fleck virus en-keyword=plant virus kn-keyword=plant virus en-keyword=rhabdovirus kn-keyword=rhabdovirus en-keyword=virus genome structure kn-keyword=virus genome structure END start-ver=1.4 cd-journal=joma no-vol=154 cd-vols= no-issue=1 article-no= start-page=37 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20081206 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification and characterization of structural proteins of orchid fleck virus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Orchid fleck virus (OFV) has a bipartite negative-sense RNA genome with sequence similarities to plant rhabdoviruses. The non-enveloped bullet-shaped particles of OFV are similar to those of the internal ribonucleoprotein (RNP)-M protein structure of rhabdoviruses, but they are about half the size of typical plant rhabdoviruses. Purified preparations contained intact bullet-shaped and filamentous particles. The filamentous particles showed a tightly coiled coil structure or a coiled structure with a helical twist, which resembles the RNP complex of rhabdoviruses. OFV bullet-shaped particles were structurally stable in solutions containing 2% Triton X-100 and 0.8 M NaCl. Western blot analyses revealed that the bullet-shaped particles contained N, P and M proteins, while filamentous particles contained mainly N and P proteins. In addition, a small amount of the L protein was detected in both types of particles. Thus, the structural proteins of OFV have properties similar to those of rhabdoviruses, except that the particles are non-enveloped and are relatively resistant to detergent-treatment under high-salt conditions. en-copyright= kn-copyright= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TamadaTetsuo en-aut-sei=Tamada en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Research Institute for Bioresources, Okayama University kn-affil= affil-num=2 en-affil=College of Bioresource Sciences, Nihon University kn-affil= affil-num=3 en-affil=Research Institute for Bioresources, Okayama University kn-affil= en-keyword=Rabies kn-keyword=Rabies en-keyword=ORF4 Protein kn-keyword=ORF4 Protein en-keyword=Sucrose Density Gradient Centrifugation kn-keyword=Sucrose Density Gradient Centrifugation en-keyword=Coil Coil Structure kn-keyword=Coil Coil Structure en-keyword=Potential Glycosylation Site kn-keyword=Potential Glycosylation Site END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=1 article-no= start-page=219 end-page=232 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Pathogenetic roles of beet necrotic yellow vein virus RNA5 in the exacerbation of symptoms and yield reduction, development of scab‐like symptoms, and Rz1‐resistance breaking in sugar beet en-subtitle= kn-subtitle= en-abstract= kn-abstract=Beet necrotic yellow vein virus (BNYVV) generally has a four‐segmented positive‐sense RNA genome (RNAs 1–4), but some European and most Asian strains have an additional segment, RNA5. This study examined the effect of RNA5 and RNA3 on different sugar beet cultivars using a Polymyxa‐mediated inoculation system under field and laboratory conditions. In field tests, the degree of sugar yield served as an index for assessing the virulence of BNYVV strains. Japanese A‐II type isolates without RNA5 caused mostly 15%–90% sugar yield reductions, depending on the susceptibility of sugar beet cultivars, whereas the isolates with RNA5 induced more than 90% yield losses in the seven susceptible cultivars, but small yield losses in one Rz1‐resistant and Rizor cultivars. However, a laboratory‐produced isolate containing RNA5 but lacking RNA3 caused higher yield losses in Rizor than in susceptible plants, and induced scab‐like symptoms on the root surface of both susceptible and resistant plants. In laboratory tests, A‐II type isolates without RNA5 had low viral RNA accumulation levels in roots of Rizor and Rz1‐resistant plants at early stages of infection, but in the presence of RNA5, viral RNA3 accumulation levels increased remarkably. This increased RNA3 accumulation was not observed in roots of the WB42 accession with the Rz2 gene. In contrast, the presence of RNA3 did not affect RNA5 accumulation levels. Collectively, this study demonstrated that RNA5 is involved in the development of scab‐like symptoms and the enhancement of RNA3 accumulation, and suggests these characteristics of RNA5 are associated with Rz1‐resistance breaking. en-copyright= kn-copyright= en-aut-name=TamadaTetsuo en-aut-sei=Tamada en-aut-mei=Tetsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UchinoHirokatsu en-aut-sei=Uchino en-aut-mei=Hirokatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KusumeToshimi en-aut-sei=Kusume en-aut-mei=Toshimi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Iketani‐SaitoMinako en-aut-sei=Iketani‐Saito en-aut-mei=Minako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ChibaSotaro en-aut-sei=Chiba en-aut-mei=Sotaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=AndikaIda Bagus en-aut-sei=Andika en-aut-mei=Ida Bagus kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=2 en-affil=Research Center, Nippon Beet Sugar Mfg. Co., Ltd. kn-affil= affil-num=3 en-affil=Hokkaido Central Agricultural Experiment Station kn-affil= affil-num=4 en-affil=Hokkaido Central Agricultural Experiment Station kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources (IPSR), Okayama University kn-affil= en-keyword=BNYVV kn-keyword=BNYVV en-keyword=resistance breaking kn-keyword=resistance breaking en-keyword=RNA5 kn-keyword=RNA5 en-keyword=Rz1 gene kn-keyword=Rz1 gene en-keyword=scab‐like symptom kn-keyword=scab‐like symptom en-keyword=sugar beet kn-keyword=sugar beet END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=1 article-no= start-page=15001 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160111 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A capsidless ssRNA virus hosted by an unrelated dsRNA virus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication1. Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix2. YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses3, while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal totiviruses4. Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+)ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus. en-copyright= kn-copyright= en-aut-name=ZhangRui en-aut-sei=Zhang en-aut-mei=Rui kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HisanoSakae en-aut-sei=Hisano en-aut-mei=Sakae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanematsuSatoko en-aut-sei=Kanematsu en-aut-mei=Satoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SuzukiNobuhiro en-aut-sei=Suzuki en-aut-mei=Nobuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=5 en-affil=NARO Institute of Fruit Tree Science kn-affil= affil-num=6 en-affil=Agrivirology Laboratory, Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=Molecular evolution kn-keyword=Molecular evolution en-keyword=Viral genetics kn-keyword=Viral genetics END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=16 article-no= start-page=4778 end-page=4796 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200506 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene en-subtitle= kn-subtitle= en-abstract= kn-abstract=Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 degrees C, moderately low storage temperatures of 5-20 degrees C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit. en-copyright= kn-copyright= en-aut-name=MitaloOscar W. en-aut-sei=Mitalo en-aut-mei=Oscar W. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OtsukiTakumi en-aut-sei=Otsuki en-aut-mei=Takumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkadaRui en-aut-sei=Okada en-aut-mei=Rui kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ObitsuSaeka en-aut-sei=Obitsu en-aut-mei=Saeka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MasudaKanae en-aut-sei=Masuda en-aut-mei=Kanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HojoYuko en-aut-sei=Hojo en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MatsuuraTakakazu en-aut-sei=Matsuura en-aut-mei=Takakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=AbeDaigo en-aut-sei=Abe en-aut-mei=Daigo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=AsicheWilliam O. en-aut-sei=Asiche en-aut-mei=William O. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=AkagiTakashi en-aut-sei=Akagi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=KuboYasutaka en-aut-sei=Kubo en-aut-mei=Yasutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=UshijimaKoichiro en-aut-sei=Ushijima en-aut-mei=Koichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=8 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=9 en-affil=National Agriculture and Food Research Organization, Shikoku Research Station kn-affil= affil-num=10 en-affil=Department of Research and Development, Del Monte Kenya Ltd kn-affil= affil-num=11 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=12 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=13 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=1-methylcyclopropene kn-keyword=1-methylcyclopropene en-keyword=carotenoids kn-keyword=carotenoids en-keyword=chlorophyll kn-keyword=chlorophyll en-keyword=Citrus limon kn-keyword=Citrus limon en-keyword=ethylene kn-keyword=ethylene en-keyword=low temperature kn-keyword=low temperature en-keyword=peel degreening kn-keyword=peel degreening en-keyword=phytohormones kn-keyword=phytohormones en-keyword=transcriptome kn-keyword=transcriptome END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ドナーの上気道微生物より、レシピエントの微生物の方がより肺移植後肺炎と関連している kn-title=Airway bacteria of the recipient but not the donor are relevant to post-lung transplant pneumonia en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KonishiYusuke en-aut-sei=Konishi en-aut-mei=Yusuke kn-aut-name=小西祐輔 kn-aut-sei=小西 kn-aut-mei=祐輔 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue=19 article-no= start-page=147 end-page=158 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=19910331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=読解・読書能力を高めるための言語・心理・生物学的基礎―失語症患者を対象とした研究― en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=森寿子 kn-aut-sei=森 kn-aut-mei=寿子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=川崎医科大学 END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=6 article-no= start-page=822 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200530 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Lanthanide-Dependent Methanol and Formaldehyde Oxidation inMethylobacterium aquaticumStrain 22A en-subtitle= kn-subtitle= en-abstract= kn-abstract=Lanthanides (Ln) are an essential cofactor for XoxF-type methanol dehydrogenases (MDHs) in Gram-negative methylotrophs. The Ln(3+)dependency of XoxF has expanded knowledge and raised new questions in methylotrophy, including the differences in characteristics of XoxF-type MDHs, their regulation, and the methylotrophic metabolism including formaldehyde oxidation. In this study, we genetically identified one set of Ln(3+)- and Ca2+-dependent MDHs (XoxF1 and MxaFI), that are involved in methylotrophy, and an ExaF-type Ln(3+)-dependent ethanol dehydrogenase, among six MDH-like genes inMethylobacterium aquaticumstrain 22A. We also identified the causative mutations in MxbD, a sensor kinase necessary formxaFexpression andxoxF1repression, for suppressive phenotypes inxoxF1mutants defective in methanol growth even in the absence of Ln(3+). Furthermore, we examined the phenotypes of a series of formaldehyde oxidation-pathway mutants (fae1,fae2,mchin the tetrahydromethanopterin (H4MPT) pathway andhgdin the glutathione-dependent formaldehyde dehydrogenase (GSH) pathway). We found that MxaF produces formaldehyde to a toxic level in the absence of the formaldehyde oxidation pathways and that either XoxF1 or ExaF can oxidize formaldehyde to alleviate formaldehyde toxicity in vivo. Furthermore, the GSH pathway has a supportive role for the net formaldehyde oxidation in addition to the H4MPT pathway that has primary importance. Studies on methylotrophy inMethylobacteriumspecies have a long history, and this study provides further insights into genetic and physiological diversity and the differences in methylotrophy within the plant-colonizing methylotrophs. en-copyright= kn-copyright= en-aut-name=YanpiratPatcha en-aut-sei=Yanpirat en-aut-mei=Patcha kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakatsujiYukari en-aut-sei=Nakatsuji en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HiragaShota en-aut-sei=Hiraga en-aut-mei=Shota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujitaniYoshiko en-aut-sei=Fujitani en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IzumiTerumi en-aut-sei=Izumi en-aut-mei=Terumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MasudaSachiko en-aut-sei=Masuda en-aut-mei=Sachiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MitsuiRyoji en-aut-sei=Mitsui en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakagawaTomoyuki en-aut-sei=Nakagawa en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=7 en-affil=Department of Biochemistry, Faculty of Science, Okayama University of Science kn-affil= affil-num=8 en-affil=The United Graduate School of Agricultural Science, Gifu University kn-affil= affil-num=9 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=lanthanide kn-keyword=lanthanide en-keyword=methylotroph kn-keyword=methylotroph en-keyword=XoxF kn-keyword=XoxF en-keyword=methanol dehydrogenase kn-keyword=methanol dehydrogenase en-keyword=Methylobacteriumspecies kn-keyword=Methylobacteriumspecies END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=5 article-no= start-page=1683 end-page=1696 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Honeydew-associated microbes elicit defense responses against brown planthopper in rice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Feeding of sucking insects, such as the rice brown planthopper (Nilaparvata lugens; BPH), causes only limited mechanical damage on plants that is otherwise essential for injury-triggered defense responses against herbivores. In pursuit of complementary BPH elicitors perceived by plants, we examined the potential effects of BPH honeydew secretions on the BPH monocot host, rice (Oryza sativa). We found that BPH honeydew strongly elicits direct and putative indirect defenses in rice, namely accumulation of phytoalexins in the leaves, and release of volatile organic compounds from the leaves that serve to attract natural enemies of herbivores, respectively. We then examined the elicitor active components in the honeydew and found that bacteria in the secretions are responsible for the activation of plant defense. Corroborating the importance of honeydew-associated microbiota for induced plant resistance, BPHs partially devoid of their microbiota via prolonged antibiotics ingestion induced significantly less defense in rice relative to antibiotic-free insects applied to similar groups of plants. Our data suggest that rice plants may additionally perceive herbivores via their honeydew-associated microbes, allowing them to discriminate between incompatible herbivores—that do not produce honeydew—and those that are compatible and therefore dangerous. en-copyright= kn-copyright= en-aut-name=WariDavid en-aut-sei=Wari en-aut-mei=David kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KabirMd Alamgir en-aut-sei=Kabir en-aut-mei=Md Alamgir kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MujionoKadis en-aut-sei=Mujiono en-aut-mei=Kadis kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HojoYuko en-aut-sei=Hojo en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShinyaTomonori en-aut-sei=Shinya en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=NakataniHiroko en-aut-sei=Nakatani en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=GalisIvan en-aut-sei=Galis en-aut-mei=Ivan kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=7 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=8 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=Honeydew-associated microorganisms kn-keyword=Honeydew-associated microorganisms en-keyword=phytoalexins kn-keyword=phytoalexins en-keyword=plant defense kn-keyword=plant defense en-keyword=rice (Oryza sativa) kn-keyword=rice (Oryza sativa) en-keyword=rice brown planthopper (Nilaparvata lugens) kn-keyword=rice brown planthopper (Nilaparvata lugens) en-keyword=sucking insect kn-keyword=sucking insect END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Spiranthes sinensis (Pers.) Ames kn-title=ネジバナ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ラン科 (Orchidaceae) kn-keyword=ラン科 (Orchidaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Eleocharis acicularis (L.) Roem. et Schult. var. longiseta Svenson kn-title=マツバイ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=カヤツリグサ科 (Cyperaceae) kn-keyword=カヤツリグサ科 (Cyperaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cyperus serotinus Rottb. kn-title=ミズガヤツリ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=カヤツリグサ科 (Cyperaceae) kn-keyword=カヤツリグサ科 (Cyperaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Echinochloa crus-galli (L.) P.Beauv. var. praticola Ohwi kn-title=ヒメイヌビエ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=イネ科 (Poaceae) kn-keyword=イネ科 (Poaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Echinochloa crus-galli (L.) P.Beauv. var. formosensis Ohwi kn-title=ヒメタイヌビエ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=イネ科 (Poaceae) kn-keyword=イネ科 (Poaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sagittaria pygmaea Miq. kn-title=ウリカワ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=オモダカ科 (Alismataceae) kn-keyword=オモダカ科 (Alismataceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Lobelia chinensis Lour. kn-title=ミゾカクシ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キキョウ科 (Campanulaceae) kn-keyword=キキョウ科 (Campanulaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Veronica peregrina L. kn-title=ムシクサ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=オオバコ科 (Plantaginaceae) kn-keyword=オオバコ科 (Plantaginaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Mazus pumilus (Burm.f.) Steenis kn-title=トキワハゼ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=サギゴケ科 (Mazaceae) kn-keyword=サギゴケ科 (Mazaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Callitriche palustris L. kn-title=ミズハコベ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=オオバコ科 (Plantaginaceae) kn-keyword=オオバコ科 (Plantaginaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Dichondra micrantha Urb. kn-title=アオイゴケ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ヒルガオ科 (Convolvulaceae) kn-keyword=ヒルガオ科 (Convolvulaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ludwigia epilobioides Maxim. subsp. epilobioides kn-title=チョウジタデ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=アカバナ科 (Onagraceae) kn-keyword=アカバナ科 (Onagraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Elatine triandra Schkuhr var. pedicellata Krylov kn-title=ミゾハコベ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ミゾハコベ科 (Elatinaceae) kn-keyword=ミゾハコベ科 (Elatinaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Viola yedoensis Makino kn-title=ノジスミレ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=スミレ科 (Violaceae) kn-keyword=スミレ科 (Violaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Oxalis corniculata L. kn-title=カタバミ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=カタバミ科 (Oxalidaceae) kn-keyword=カタバミ科 (Oxalidaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Rorippa cantoniensis (Lour.) Ohwi kn-title=コイヌガラシ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=アブラナ科 (Brassicaceae) kn-keyword=アブラナ科 (Brassicaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Achyranthes bidentata Blume var. fauriei (H.Lév. et Vaniot) kn-title=ヒナタイノコヅチ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ヒユ科 (Amaranthaceae) kn-keyword=ヒユ科 (Amaranthaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Persicaria lapathifolia (L.) Delarbre var. incana (Roth) H.Hara kn-title=サナエタデ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=タデ科 (Polygonaceae) kn-keyword=タデ科 (Polygonaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fatoua villosa (Thunb.) Nakai kn-title=クワクサ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=クワ科 (Moraceae) kn-keyword=クワ科 (Moraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pistia stratiotes L. kn-title=ボタンウキクサ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=サトイモ科 (Araceae) kn-keyword=サトイモ科 (Araceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Taraxacum laevigatum (Willd.) DC. kn-title=アカミタンポポ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Solidago gigantea Aiton subsp. serotina (Kuntze) McNeill kn-title=オオアワダチソウ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Senecio madagascariensis Poir. kn-title=ナルトサワギク en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Rudbeckia laciniata L. kn-title=オオハンゴンソウ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Gymnocoronis spilanthoides (D.Don ex Hook. et Arn.) DC. kn-title=ミズヒマワリ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Gamochaeta purpurea (L.) Cabrera kn-title=ウスベニチチコグサ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Galinsoga quadriradiata Ruiz et Pav. kn-title=ハキダメギク en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Eclipta alba (L.) Hassk. kn-title=アメリカタカサブロウ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Coreopsis lanceolata L. kn-title=オオキンケイギク en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Symphyotrichum subulatum (Michx.) G.L.Nesom var. elongatum (Bosserdet ex A.G.Jones et Lowry) S.D.Sundberg kn-title=オオホウキギク en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キク科 (Asteraceae) kn-keyword=キク科 (Asteraceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Plantago virginica L. kn-title=ツボミオオバコ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=オオバコ科 (Plantaginaceae) kn-keyword=オオバコ科 (Plantaginaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Veronica anagallis-aquatica L. kn-title=オオカワヂシャ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=オオバコ科 (Plantaginaceae) kn-keyword=オオバコ科 (Plantaginaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Salpichroa origanifolia (Lam.) Baill. kn-title=ハコベホオズキ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ナス科 (Solanaceae) kn-keyword=ナス科 (Solanaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Stachys arvensis (L.) L. kn-title=ヤブチョロギ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=シソ科 (Lamiaceae) kn-keyword=シソ科 (Lamiaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ipomoea pes-tigridis L. kn-title=キクザアサガオ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ヒルガオ科 (Convolvulaceae) kn-keyword=ヒルガオ科 (Convolvulaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sicyos angulatus L. kn-title=アレチウリ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ウリ科 (Cucurbitaceae) kn-keyword=ウリ科 (Cucurbitaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Desmodium paniculatum (L.) DC. kn-title=アレチヌスビトハギ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=マメ科 (Fabaceae) kn-keyword=マメ科 (Fabaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Lepidium draba L. kn-title=アコウグンバイ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=アブラナ科 (Brassicaceae) kn-keyword=アブラナ科 (Brassicaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Ranunculus muricatus L. kn-title=トゲミノキツネノボタン en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=キンポウゲ科 (Ranunculaceae) kn-keyword=キンポウゲ科 (Ranunculaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Alternanthera sessilis (L.) R.Br. ex DC. kn-title=ツルノゲイトウ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ヒユ科 (Amaranthaceae) kn-keyword=ヒユ科 (Amaranthaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Stellaria media (L.) Vill. kn-title=コハコベ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ナデシコ科 (Caryophyllaceae) kn-keyword=ナデシコ科 (Caryophyllaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Eudianthe coeli-rosa (L.) Endl. kn-title=コムギセンノウ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ナデシコ科 (Caryophyllaceae) kn-keyword=ナデシコ科 (Caryophyllaceae) END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year= dt-pub= dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sagina apetala Ard. kn-title=イトツメクサ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-keyword=ナデシコ科 (Caryophyllaceae) kn-keyword=ナデシコ科 (Caryophyllaceae) END start-ver=1.4 cd-journal=joma no-vol=247 cd-vols= no-issue= article-no= start-page=125933 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200116 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Application of the cellular oxidation biosensor to Toxicity Identification Evaluations for high-throughput toxicity assessment of river water en-subtitle= kn-subtitle= en-abstract= kn-abstract=Toxicity Identification Evaluation (TIE) is a useful method for the classification and identification of toxicants in a composite environment water sample. However, its extension to a larger sample size has been restrained owing to the limited throughput of toxicity bioassays. Here we reported the development of a high-throughput method of TIE Phase I. This newly developed method was assisted by the fluorescence-based cellular oxidation (CO) biosensor fabricated with roGFP2-expressing bacterial cells in 96-well microplate format. The assessment of four river water samples from Langat river basin by this new method demonstrated that the contaminant composition of the four samples can be classified into two distinct groups. The entire toxicity assay consisted of 2338 tests was completed within 12 h with a fluorescence microplate reader. Concurrently, the sample volume for each assay was reduced to 50 μL, which is 600 to 4700 times lesser to compare with conventional bioassays. These imply that the throughput of the CO biosensor-assisted TIE Phase I is now feasible for constructing a large-scale toxicity monitoring system, which would cover a whole watershed scale. en-copyright= kn-copyright= en-aut-name=OoiLia en-aut-sei=Ooi en-aut-mei=Lia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkazakiKeisuke en-aut-sei=Okazaki en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Arias-BarreiroCarlos R. en-aut-sei=Arias-Barreiro en-aut-mei=Carlos R. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HengLee Yook en-aut-sei=Heng en-aut-mei=Lee Yook kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=nstitute of Plant Science and Resources, Okayama University kn-affil= affil-num=4 en-affil=Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), The National University of Malaysia kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=High-throughput cytotoxicity biosensor kn-keyword=High-throughput cytotoxicity biosensor en-keyword=Toxicity identification evaluation kn-keyword=Toxicity identification evaluation en-keyword=River water pollution kn-keyword=River water pollution en-keyword=Ecotoxicity management kn-keyword=Ecotoxicity management en-keyword=Integrated watershed management kn-keyword=Integrated watershed management END start-ver=1.4 cd-journal=joma no-vol=2018 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Bulletin of Archaeological Research Center Okayama University 2018 kn-title=岡山大学埋蔵文化財調査研究センター紀要2018 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MinamiKentaro en-aut-sei=Minami en-aut-mei=Kentaro kn-aut-name=南健太郎 kn-aut-sei=南 kn-aut-mei=健太郎 aut-affil-num=1 ORCID= en-aut-name=IwasakiShiho en-aut-sei=Iwasaki en-aut-mei=Shiho kn-aut-name=岩﨑志保 kn-aut-sei=岩﨑 kn-aut-mei=志保 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=那須浩郞 kn-aut-sei=那須 kn-aut-mei=浩郞 aut-affil-num=3 ORCID= en-aut-name=YamamotoEtsuyo en-aut-sei=Yamamoto en-aut-mei=Etsuyo kn-aut-name=山本悦世 kn-aut-sei=山本 kn-aut-mei=悦世 aut-affil-num=4 ORCID= en-aut-name=YamaguchiYuji en-aut-sei=Yamaguchi en-aut-mei=Yuji kn-aut-name=山口雄治 kn-aut-sei=山口 kn-aut-mei=雄治 aut-affil-num=5 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=富岡直人 kn-aut-sei=富岡 kn-aut-mei=直人 aut-affil-num=6 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=米田穣 kn-aut-sei=米田 kn-aut-mei=穣 aut-affil-num=7 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=大久保徹也 kn-aut-sei=大久保 kn-aut-mei=徹也 aut-affil-num=8 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=藁科哲男 kn-aut-sei=藁科 kn-aut-mei=哲男 aut-affil-num=9 ORCID= en-aut-name=NozakiTakahiro en-aut-sei=Nozaki en-aut-mei=Takahiro kn-aut-name=野崎貴博 kn-aut-sei=野崎 kn-aut-mei=貴博 aut-affil-num=10 ORCID= affil-num=1 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=2 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=3 en-affil= kn-affil=岡山理科大学生物地球学部 affil-num=4 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=5 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=6 en-affil= kn-affil=岡山理科大学生物地球学部 affil-num=7 en-affil= kn-affil=東京大学総合研究博物館 affil-num=8 en-affil= kn-affil=徳島文理大学 affil-num=9 en-affil= kn-affil=遺物材料研究所 affil-num=10 en-affil=Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター END start-ver=1.4 cd-journal=joma no-vol=2017 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190329 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Bulletin of Archaeological Research Center Okayama University 2017 kn-title=岡山大学埋蔵文化財調査研究センター紀要2017 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NozakiTakahiro en-aut-sei=Nozaki en-aut-mei=Takahiro kn-aut-name=野崎貴博 kn-aut-sei=野崎 kn-aut-mei=貴博 aut-affil-num=1 ORCID= en-aut-name=MinamiKentaro en-aut-sei=Minami en-aut-mei=Kentaro kn-aut-name=南健太郎 kn-aut-sei=南 kn-aut-mei=健太郎 aut-affil-num=2 ORCID= en-aut-name=YamamotoEtsuyo en-aut-sei=Yamamoto en-aut-mei=Etsuyo kn-aut-name=山本悦世 kn-aut-sei=山本 kn-aut-mei=悦世 aut-affil-num=3 ORCID= en-aut-name=SuzukiShigeyuki en-aut-sei=Suzuki en-aut-mei=Shigeyuki kn-aut-name=鈴木茂之 kn-aut-sei=鈴木 kn-aut-mei=茂之 aut-affil-num=4 ORCID= en-aut-name=YamaguchiYuji en-aut-sei=Yamaguchi en-aut-mei=Yuji kn-aut-name=山口雄治 kn-aut-sei=山口 kn-aut-mei=雄治 aut-affil-num=5 ORCID= en-aut-name=IwasakiShiho en-aut-sei=Iwasaki en-aut-mei=Shiho kn-aut-name=岩﨑志保 kn-aut-sei=岩﨑 kn-aut-mei=志保 aut-affil-num=6 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=古環境センター kn-aut-sei=古環境センター kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=吉田生物研究所 kn-aut-sei=吉田生物研究所 kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=2 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=3 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=4 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学 affil-num=5 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=6 en-affil= Archaeological Research Center, Okayama University kn-affil=岡山大学埋蔵文化財調査研究センター affil-num=7 en-affil= kn-affil= affil-num=8 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=109 cd-vols= no-issue= article-no= start-page=29 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Development of Effective Biological Treatment Process for Acid Mine Drainage kn-title=酸性鉱山廃水の 効果的な生物的処理プロセスの開発 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed. en-copyright= kn-copyright= en-aut-name=KamimuraKazuo en-aut-sei=Kamimura en-aut-mei=Kazuo kn-aut-name=上村一雄 kn-aut-sei=上村 kn-aut-mei=一雄 aut-affil-num=1 ORCID= en-aut-name=KanaoTadayoshi en-aut-sei=Kanao en-aut-mei=Tadayoshi kn-aut-name=金尾忠芳 kn-aut-sei=金尾 kn-aut-mei=忠芳 aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 en-keyword=acid mine drainage kn-keyword=acid mine drainage en-keyword=bioremediation kn-keyword=bioremediation en-keyword=iron-oxidizing bacteria kn-keyword=iron-oxidizing bacteria en-keyword=microbial community kn-keyword=microbial community en-keyword=Acidithiobacillus ferrooxidans kn-keyword=Acidithiobacillus ferrooxidans END start-ver=1.4 cd-journal=joma no-vol=42 cd-vols= no-issue=2 article-no= start-page=437 end-page=447 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180716 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The mechanism of SO2 -induced stomatal closure differs from O3 and CO2 responses and is mediated by nonapoptotic cell death in guard cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract= Plants closing stomata in the presence of harmful gases is believed to be a stress avoidance mechanism. SO2 , one of the major airborne pollutants, has long been reported to induce stomatal closure, yet the mechanism remains unknown. Little is known about the stomatal response to airborne pollutants besides O3 . SLOW ANION CHANNEL-ASSOCIATED 1 (SLAC1) and OPEN STOMATA 1 (OST1) were identified as genes mediating O3 -induced closure. SLAC1 and OST1 are also known to mediate stomatal closure in response to CO2 , together with RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs). The overlaying roles of these genes in response to O3 and CO2 suggested that plants share their molecular regulators for airborne stimuli. Here, we investigated and compared stomatal closure event induced by a wide concentration range of SO2 in Arabidopsis through molecular genetic approaches. O3 - and CO2 -insensitive stomata mutants did not show significant differences from the wild type in stomatal sensitivity, guard cell viability, and chlorophyll content revealing that SO2 -induced closure is not regulated by the same molecular mechanisms as for O3 and CO2 . Nonapoptotic cell death is shown as the reason for SO2 -induced closure, which proposed the closure as a physicochemical process resulted from SO2 distress, instead of a biological protection mechanism. en-copyright= kn-copyright= en-aut-name=Ooi Lia en-aut-sei=Ooi en-aut-mei=Lia kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuuraTakakazu en-aut-sei=Matsuura en-aut-mei=Takakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MunemasaShintaro en-aut-sei=Munemasa en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurataYoshiyuki en-aut-sei=Murata en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatsuharaMaki en-aut-sei=Katsuhara en-aut-mei=Maki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HirayamaTakashi en-aut-sei=Hirayama en-aut-mei=Takashi kn-aut-name=平山隆志 kn-aut-sei=平山 kn-aut-mei=隆志 aut-affil-num=6 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=2 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=7 en-affil= Institute of Plant Science and Resources, Okayama University kn-affil= en-keyword=airborne pollutants kn-keyword=airborne pollutants en-keyword=nonapoptotic cell death kn-keyword=nonapoptotic cell death en-keyword=stomatal closure kn-keyword=stomatal closure en-keyword=sulfur dioxide kn-keyword=sulfur dioxide END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=生物分解性都市ごみを対象とした高速二段嫌気性消化モデルの開発 kn-title=Developing a High-Rate Two-Stage Anaerobic Digestion Model to Deal with Biodegradable Municipal Solid Waste en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=DINHPHAM VAN en-aut-sei=DINH en-aut-mei=PHAM VAN kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol=131 cd-vols= no-issue=2 article-no= start-page=75 end-page=77 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The 2018 Incentive Award of the Okayama Medical Association in Cancer Research (2018 Hayashibara Prize and Yamada Prize) kn-title=平成30年度岡山医学会賞 がん研究奨励賞（林原賞・山田賞） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KinoshitaRie en-aut-sei=Kinoshita en-aut-mei=Rie kn-aut-name=木下理恵 kn-aut-sei=木下 kn-aut-mei=理恵 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生物学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=複素環式生物活性化合物の合成研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AndoJunki en-aut-sei=Ando en-aut-mei=Junki kn-aut-name=安藤潤紀 kn-aut-sei=安藤 kn-aut-mei=潤紀 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=マウス歯胚の発育に対する抗がん剤の影響についての組織学的および分子生物学的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsunodaYoko en-aut-sei=Tsunoda en-aut-mei=Yoko kn-aut-name=角田陽子 kn-aut-sei=角田 kn-aut-mei=陽子 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol=130 cd-vols= no-issue=3 article-no= start-page=135 end-page=139 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20181203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Basic studies on S100-mediated cancer metastasis based on the development of innovative biologics to aim at its effective prevention kn-title=S100タンパク質に着眼したがん転移機構の解明とその制御 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaguchiMasakiyo en-aut-sei=Sakaguchi en-aut-mei=Masakiyo kn-aut-name=阪口政清 kn-aut-sei=阪口 kn-aut-mei=政清 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生物学 END start-ver=1.4 cd-journal=joma no-vol=170 cd-vols= no-issue= article-no= start-page=41 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190222 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=How Is the Counselor Involved When System Support Is Effective? : How to Make a Team that Functions as One System kn-title=効果的なチーム支援を引き出すカウンセラーの関わり方 : システムとして機能するチームをつくるために en-subtitle= kn-subtitle= en-abstract= kn-abstract=　人が生物学的な存在であると同時に社会的な存在であることを考えると，カウンセラーが個人を支援しようとする場合，個別の支援の他に、その個人を取り巻く様々な援助資源をうまく機能させて，チームで支援することも不可欠となる。では，効果的なチーム支援を行うために，カウンセラーはどのように関わればよいのだろうか。本稿では，カウンセラーがチーム支援を引き出した成功例として３つの実践を取り上げた。具体的には高等学校内での転入職員の支援，大学の学生相談での長期欠席者の支援，児童養護施設での児童の支援と，クライエントの援助資源が異なる３つである。しかし，そこでのカウンセラーのかかわり方には，ある共通点が見いだせた。本稿では，その共通点を箕口（2016）の「コミュニティ・アプローチを実践する心理援助の専門家の要件」に沿って考察した。最後に「協働」「どのようにサービスを提供するのか」という観点から考察した。 en-copyright= kn-copyright= en-aut-name=AokiTazuko en-aut-sei=Aoki en-aut-mei=Tazuko kn-aut-name=青木多寿子 kn-aut-sei=青木 kn-aut-mei=多寿子 aut-affil-num=1 ORCID= en-aut-name=NakatomiTakahiro en-aut-sei=Nakatomi en-aut-mei=Takahiro kn-aut-name=中富尚宏 kn-aut-sei=中富 kn-aut-mei=尚宏 aut-affil-num=2 ORCID= en-aut-name=TokudaTomoyo en-aut-sei=Tokuda en-aut-mei=Tomoyo kn-aut-name=徳田智代 kn-aut-sei=徳田 kn-aut-mei=智代 aut-affil-num=3 ORCID= en-aut-name=MorimotoAtsushi en-aut-sei=Morimoto en-aut-mei=Atsushi kn-aut-name=森本篤 kn-aut-sei=森本 kn-aut-mei=篤 aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科 affil-num=2 en-affil=Esperance Yokkaichi children’s home kn-affil=乳児院・児童養護施設施エスペランス四日市 affil-num=3 en-affil=A Faculty of Literature, Kurume University kn-affil=久留米大学文学部心理学科 affil-num=4 en-affil=Okayama Joto Senior High School kn-affil=岡山県立岡山城東高校 en-keyword=チーム支援 kn-keyword=チーム支援 en-keyword=カウンセラーの関わり方 kn-keyword=カウンセラーの関わり方 en-keyword=システム kn-keyword=システム en-keyword=実践例 kn-keyword=実践例 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180927 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ショウジョウバエの蛹化タイミングを決める生物タイマーシステムに影響を及ぼす分子 kn-title=Molecular players affecting the biological timer system to determine pupation timing in Drosophila en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Hamdy Aly Mohamed Aly en-aut-sei=Hamdy Aly Mohamed Aly en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=34 cd-vols= no-issue= article-no= start-page=2 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=201804 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Transcription factor Runx3 in the mouse hypothalamo-pituitary-gonadal system kn-title=マウス視床下部・下垂体・生殖腺系における転写因子Runx3の役割 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Runx3 is a transcription factor that belongs to the Runx family. Female Runx3 knockout (Runx3−⁄−) mouse was anovulatory and infertile. Ovarian transplantation experiment suggested that lack of ovulation in Runx3−⁄− mice was caused by alteration of gonadotropin secretion in Runx3−⁄− mice. Cyp11a1 mRNA expression was less in Runx3−⁄− mouse ovaries than in wt ones. Hypothalamic Gnrh1 mRNA was increased, and Kiss1 mRNA expression in the anteroventral periventricular nucleus was decreased, but Kisspeptin mRNA in the arcuate nucleus was increased in Runx3-/- mice. Pituitary Fshb mRNA levels were increased in Runx3−⁄− mice. Cholesterol side-chain cleavage enzyme gene (Cyp11a1) expression was decreased in ovaries of Runx3−⁄− mice. These findings suggest that anovulation in Runx3−⁄− mice was partly due to the alterations in hypothalamus-pituitary-ovary system. Runx3 plays a key role in female reproduction through alteration of gonadotropin secretion. en-copyright= kn-copyright= en-aut-name=TakahashiSumio en-aut-sei=Takahashi en-aut-mei=Sumio kn-aut-name=高橋純夫 kn-aut-sei=高橋 kn-aut-mei=純夫 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科高次生物科学講座･生体統御学分野 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Aspergillus ustus 由来の生理活性化合物が有する独特の抗微生物活性 kn-title=Unique antimicrobial activity of bioactive compound produced by Aspergillus ustus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=NatthapatSohsomboon kn-aut-sei=Natthapat kn-aut-mei=Sohsomboon aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=キイロショウジョウバエの変態期における発生タイミングを決める生物タイマーの分子機構と栄養シグナルの影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NishidaHaruka en-aut-sei=Nishida en-aut-mei=Haruka kn-aut-name=西田遥 kn-aut-sei=西田 kn-aut-mei=遥 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=20180323 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Streptococcus mutans における分子シャペロンDnaKの分子生物学的解明 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YoshidaEri en-aut-sei=Yoshida en-aut-mei=Eri kn-aut-name=吉田衣里 kn-aut-sei=吉田 kn-aut-mei=衣里 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170929 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ベトナム酪農の制限要因及び発展性に関する微生物学的研究 kn-title=Microbiological studies on constraints and opportunities of dairy cattle production in Vietnam en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Tran Thi Minh Tu en-aut-sei=Tran Thi Minh Tu en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol=57 cd-vols= no-issue=8 article-no= start-page=1779 end-page=1990 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Involvement of OST1 Protein Kinase and PYR/PYL/RCAR Receptors in Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis Guard Cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= Methyl jasmonate (MeJA) induces stomatal closure. It has been shown that stomata of many ABA-insensitive mutants are also insensitive to MeJA, and a low amount of ABA is a prerequisite for the MeJA response. However, the molecular mechanisms of the interaction between ABA and MeJA signaling remain to be elucidated. Here we studied the interplay of signaling of the two hormones in guard cells using the quadruple ABA receptor mutant pyr1 pyl1 pyl2 pyl4 and ABA-activated protein kinase mutants ost1-2 and srk2e. In the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, nitric oxide (NO) production, cytosolic alkalization and plasma membrane Ca(2+)-permeable current (ICa) activation were not impaired. At the same time, the inactivation of the inward-rectifying K(+) current was impaired. In contrast to the quadruple mutant, MeJA-induced stomatal closure, H2O2 production, NO production and cytosolic alkalization were impaired in ost1-2 and srk2e as well as in aba2-2, the ABA-deficient mutant. The activation of ICa was also impaired in srk2e. Collectively, these results indicated that OST1 was essential for MeJA-induced stomatal closure, while PYR1, PYL1, PYL2 and PYL4 ABA receptors were not sufficient factors. MeJA did not appear to activate OST1 kinase activity. This implies that OST1 mediates MeJA signaling through an undetectable level of activity or a non-enzymatic action. MeJA induced the expression of an ABA synthesis gene, NCED3, and increased ABA contents only modestly. Taken together with previous reports, this study suggests that MeJA signaling in guard cells is primed by ABA and is not brought about through the pathway mediated by PYR1, PYL1 PYL2 and PYL4. en-copyright= kn-copyright= en-aut-name=YinYe en-aut-sei=Yin en-aut-mei=Ye kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=AdachiYuji en-aut-sei=Adachi en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraYoshimasa en-aut-sei=Nakamura en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MunemasaShintaro en-aut-sei=Munemasa en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurataYoshiyuki en-aut-sei=Murata en-aut-mei=Yoshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Institute of Plant Science and Resources, Okayama University kn-affil= affil-num=6 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=ABA kn-keyword=ABA en-keyword=ABA receptors kn-keyword=ABA receptors en-keyword=Arabidopsis thaliana kn-keyword=Arabidopsis thaliana en-keyword=Guard cells kn-keyword=Guard cells en-keyword=Methyl jasmonate kn-keyword=Methyl jasmonate en-keyword=OST1 protein kinase kn-keyword=OST1 protein kinase END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=7 article-no= start-page=1422 end-page=1431 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201207 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of hepatitis C virus production reporter-assay systems using two different hepatoma cell lines en-subtitle= kn-subtitle= en-abstract= kn-abstract=　A hepatitis C virus (HCV) infection system was developed previously using the HCV JFH-1 strain (genotype 2a) and HuH-7 cells, and this cell culture is so far the only robust production system for HCV. In patients with chronic hepatitis C, the virological effects of pegylated interferon and ribavirin therapy differ depending on the HCV strain and the genetic background of the host. Recently, we reported the hepatoma-derived Li23 cell line, in which the JFH-1 life cycle is reproduced at a level almost equal to that in HuH-7-derived RSc cells. To monitor the HCV life cycle more easily, we here developed JFH-1 reporter-assay systems using both HuH-7- and Li23-derived cell lines. To identify any genetic mutations by long-term cell culture, HCV RNAs in HuH-7 cells were amplified 130 days after infection and subjected to sequence analysis to find adaptive mutation(s) for robust virus replication. We identified two mutations, H2505Q and V2995L, in the NS5B region. V2995L but not H2505Q enhanced JFH-1 RNA replication. However, we found that H2505Q but not V2995L enhanced HCV RNA replication of strain O (genotype 1b). We also selected highly permissive D7 cells by serial subcloning of Li23 cells. The expression levels of claudin-1 and Niemann-Pick C1-like 1 in D7 cells are higher than those in parental Li23 cells. In this study, we developed HCV JFH-1 reporter-assay systems using two distinct hepatoma cell lines, HuH-7 and Li23. The mutations in NS5B resulted in different effects on strains O and JFH-1 HCV RNA replication. en-copyright= kn-copyright= en-aut-name=TakedaMidori en-aut-sei=Takeda en-aut-mei=Midori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AriumiYasuo en-aut-sei=Ariumi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WakitaTakaji en-aut-sei=Wakita en-aut-mei=Takaji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Tumor Virology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Tumor Virology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Tumor Virology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Virology II, National Institute of Infectious Disease kn-affil= affil-num=5 en-affil=Department of Tumor Virology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= END start-ver=1.4 cd-journal=joma no-vol=167 cd-vols= no-issue=1 article-no= start-page=74 end-page=85 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201207 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Identification of host genes showing differential expression profiles with cell-based long-term replication of hepatitis C virus RNA en-subtitle= kn-subtitle= en-abstract= kn-abstract=　Persistent hepatitis C virus (HCV) infection frequently causes hepatocellular carcinoma. However, the mechanisms of HCV-associated hepatocarcinogenesis and disease progression are unclear. Although the human hepatoma cell line, HuH-7, has been widely used as the only cell culture system for robust HCV replication, we recently developed new human hepatoma Li23 cell line-derived OL, OL8, OL11, and OL14 cells, in which genome-length HCV RNA (O strain of genotype 1b) efficiently replicates. OL, OL8, OL11, and OL14 cells were cultured for more than 2 years. We prepared cured cells from OL8 and OL11 cells by interferon-γ treatment. The cured cells were also cultured for more than 2 years. cDNA microarray and RT-PCR analyses were performed using total RNAs prepared from these cells. We first selected several hundred highly or moderately expressed probes, the expression levels of which were upregulated or downregulated at ratios of more than 2 or less than 0.5 in each set of compared cells (e.g., parent OL8 cells versus OL8 cells cultured for 2 years). From among these probes, we next selected those whose expression levels commonly changed during a 2-year culture of genome-length HCV RNA-replicating cells, but which did not change during a 2-year culture period in cured cells. We further examined the expression levels of the selected candidate genes by RT-PCR analysis using additional specimens from the cells cultured for 3.5 years. Reproducibility of the RT-PCR analysis using specimens from recultured cells was also confirmed. Finally, we identified 5 upregulated genes and 4 downregulated genes, the expression levels of which were irreversibly altered during 3.5-year replication of HCV RNA. These genes may play roles in the optimization of the environment in HCV RNA replication, or may play key roles in the progression of HCV-associated hepatic diseases. en-copyright= kn-copyright= en-aut-name=SejimaHiroe en-aut-sei=Sejima en-aut-mei=Hiroe kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoriKyoko en-aut-sei=Mori en-aut-mei=Kyoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AriumiYasuo en-aut-sei=Ariumi en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=HCV kn-keyword=HCV en-keyword=HCV RNA replication system kn-keyword=HCV RNA replication system en-keyword=Li23 cells kn-keyword=Li23 cells en-keyword=Long-term RNA replication kn-keyword=Long-term RNA replication en-keyword=Upregulated host genes kn-keyword=Upregulated host genes en-keyword=Downregulated host genes kn-keyword=Downregulated host genes END start-ver=1.4 cd-journal=joma no-vol=129 cd-vols= no-issue=2 article-no= start-page=81 end-page=83 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The 2016 Incentive Award of the Okayama Medical Association in General Medical Science (2016 Yuuki Prize) kn-title=平成28年度岡山医学会賞　総合研究奨励賞（結城賞） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakaguchiMasakiyo en-aut-sei=Sakaguchi en-aut-mei=Masakiyo kn-aut-name=阪口政清 kn-aut-sei=阪口 kn-aut-mei=政清 aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Cell Biolgy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生物学 END start-ver=1.4 cd-journal=joma no-vol=33 cd-vols= no-issue= article-no= start-page=31 end-page=34 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=201704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis kn-title=下垂体隆起部の発生期に特異的に発現する遺伝子Cytokine-like 1,Gap junction protein alpha 5の同定 en-subtitle= kn-subtitle= en-abstract= kn-abstract= Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development. en-copyright= kn-copyright= en-aut-name=AizawaSayaka en-aut-sei=Aizawa en-aut-mei=Sayaka kn-aut-name=相澤清香 kn-aut-sei=相澤 kn-aut-mei=清香 aut-affil-num=1 ORCID= en-aut-name=HigakiYuriko en-aut-sei=Higaki en-aut-mei=Yuriko kn-aut-name=檜垣佑理子 kn-aut-sei=檜垣 kn-aut-mei=佑理子 aut-affil-num=2 ORCID= en-aut-name=OgoshiMaho en-aut-sei=Ogoshi en-aut-mei=Maho kn-aut-name=御輿真穂 kn-aut-sei=御輿 kn-aut-mei=真穂 aut-affil-num=3 ORCID= en-aut-name=TakeuchiSakae en-aut-sei=Takeuchi en-aut-mei=Sakae kn-aut-name=竹内栄 kn-aut-sei=竹内 kn-aut-mei=栄 aut-affil-num=4 ORCID= en-aut-name=TakahashiSumio en-aut-sei=Takahashi en-aut-mei=Sumio kn-aut-name=高橋純夫 kn-aut-sei=高橋 kn-aut-mei=純夫 aut-affil-num=5 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 affil-num=2 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 affil-num=3 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科 affil-num=4 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科高次生物科学講座･生体統御学分野 affil-num=5 en-affil=Graduate School of Natural Science and Technology, Okayama University kn-affil=岡山大学大学院自然科学研究科高次生物科学講座･生体統御学分野 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=微生物由来の非晶質鉄酸化物を用いた有機合成反応の開発 kn-title=Development of Organic Reactions with Bacteriogenic Amorphous Iron Oxide en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MandaiKyoko en-aut-sei=Mandai en-aut-mei=Kyoko kn-aut-name=萬代恭子 kn-aut-sei=萬代 kn-aut-mei=恭子 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Tchnology, Okayama University kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=西日本のブナ林におけるブナ実生の生残に影響を与える非生物的要因と生物的要因 kn-title=Abiotic and biotic factors affecting the seedling survival of Fagus crenata in beech forests in western Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AkajiYasuaki en-aut-sei=Akaji en-aut-mei=Yasuaki kn-aut-name=赤路康朗 kn-aut-sei=赤路 kn-aut-mei=康朗 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=完全型と不完全型円板状外側半月の組織学的・生物学的解析 kn-title=Histological and biological comparisons between complete and incomplete discoid lateral meniscus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=InoueHiroto en-aut-sei=Inoue en-aut-mei=Hiroto kn-aut-name=井上博登 kn-aut-sei=井上 kn-aut-mei=博登 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=新規T細胞株HOZOTによる生物製剤の腫瘍特異的デリバリー kn-title=Tumor-specific delivery of biologics by a novel T-cell line HOZOT en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=OnishiTeppei en-aut-sei=Onishi en-aut-mei=Teppei kn-aut-name=大西哲平 kn-aut-sei=大西 kn-aut-mei=哲平 aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil=岡山大学大学院医歯薬学総合研究科 END start-ver=1.4 cd-journal=joma no-vol=163 cd-vols= no-issue= article-no= start-page=69 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20161128 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Differences in the Biological Sciences Education in Upper Secondary Level between Japan and Swiss German Zone - A Comparative Study between the Recent Textbooks in Basic Stage- kn-title=日本とスイス−ドイツ語圏−の後期中等教育段階における生物教育 ─ 生物基礎教科書の比較考察 ─ en-subtitle= kn-subtitle= en-abstract= kn-abstract=スイス−ドイツ語圏−のギムナジウムと日本の高等学校とにおける生物基礎に焦点を当て，後期中等教育段階における生物教育の比較考察を行った。スイスの生物基礎は，重点生物・補充生物とは種類が異なるとともに，それらの基礎，日本の生物基礎は，生物履修前の基礎である。そもそも，学習指導要領などが指示しているスイスと日本の生物基礎は，履修年限（3か1），単位数（6か2），指示する内容分野の違いから，大きく異なっていた。教科書も，その目次からは，学習内容の順序・範囲などが大きく異なっていること，索引からは，ともに現代社会の諸問題の理解に必要な要素は含まれているが，スイスでは1/ 20，日本では1/6程度しか重複（共通）していないことなどで，大きく異なっていることを明らかにした。 en-copyright= kn-copyright= en-aut-name=TanakaKenji en-aut-sei=Tanaka en-aut-mei=Kenji kn-aut-name=田中賢二 kn-aut-sei=田中 kn-aut-mei=賢二 aut-affil-num=1 ORCID= en-aut-name=TanakaKeita en-aut-sei=Tanaka en-aut-mei=Keita kn-aut-name=田中啓太 kn-aut-sei=田中 kn-aut-mei=啓太 aut-affil-num=2 ORCID= affil-num=1 en-affil=Professor Emeritus of Graduate School of Education, Okayama University kn-affil=岡山大学大学院教育学研究科名誉教授 affil-num=2 en-affil=Hisainishi Junior High School kn-affil=津市立久居西中学校 en-keyword=日本 kn-keyword=日本 en-keyword=スイス−ドイツ語圏 kn-keyword=スイス−ドイツ語圏 en-keyword=後期中等教育段階 kn-keyword=後期中等教育段階 en-keyword=生物教育 kn-keyword=生物教育 en-keyword=教科書比較 kn-keyword=教科書比較 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=アルツハイマー病治療薬を指向した新規γ-セクレターゼ調節薬のデザインと合成ならびに生物学的評価 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakaiTakafumi en-aut-sei=Takai en-aut-mei=Takafumi kn-aut-name=高井隆文 kn-aut-sei=高井 kn-aut-mei=隆文 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page=55 end-page=74 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20151230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=“情(Qing)”in the Zhu Xi Philosophy kn-title=朱子の「情」 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　本稿では、朱子のいう「情」についてできるだけ深く掘り下げて再検討することを試みる。 　「天地の情」は、自然界の、「公正無私、健やか、和やか」といった性格と気の働きとの両義を併せ持つものである。つまり、自然界のあらゆる自然現象や自然物は皆、「公正無私、健やか、和やか」といった性格を持つ気の働きによって作り出されたものである。「公正無私、健やか、和やか」という性格は、「天地の心」が無生物や生物や人間に賦与されることに伴ってそれらの物体にも備わるのである。 　「物の情」は、物体のその自身の性質を現す働きである。「草木禽獣の情」は、「五常の性」から発生した原初的「情」と、原初的「情」が具体的な物に対して向かうことによって生じた志向的情感、及び知覚、といった内面的な働きである。 　「人の情」には、「本然の性」から発生した「四端」と「気質の性」から発生した「七情」の二種類がある。「四端」は原初的「情」であり、「七情」はその一部分が原初的「情」となって現れる。原初的「情」の働き方によって生じた幾つかの異なる心の働きが派生的「情」であり、派生的「情」には「意」「志」「知」「思慮」などといった要素が含まれている。 　「意」と「志」はともに志向的情感であるが、その性格は「志は剛、意は柔」のように正反対であり、意欲、作用、忖度、感応が「意」の具体的な内容である。「知」はつまり「知覚」または「知識」であり、「知覚」は、神経感覚・器官感覚・生命感覚・道徳感覚を意味し、「知識」は認識や見識を意味する。「思慮」はつまり「思」であり、思惟・思索を意味し、派生的「情」においては枢要な働きを担うものであるが、不善を生ずるものと認識されている。 en-copyright= kn-copyright= en-aut-name=SunLuyi en-aut-sei=Sun en-aut-mei=Luyi kn-aut-name=孫路易 kn-aut-sei=孫 kn-aut-mei=路易 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学言語教育センター en-keyword=天地の情 kn-keyword=天地の情 en-keyword=物の情 kn-keyword=物の情 en-keyword=草木禽獣の情 kn-keyword=草木禽獣の情 en-keyword=人の情 kn-keyword=人の情 en-keyword=四端 kn-keyword=四端 en-keyword=七情 kn-keyword=七情 en-keyword=情 kn-keyword=情 en-keyword=意 kn-keyword=意 en-keyword=知覚 kn-keyword=知覚 en-keyword=思慮 kn-keyword=思慮 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=キウイフルーツ果実のエチレンによる成熟誘導と低温による成熟促進に関する生理学的・分子生物学的機構解析 kn-title=Elucidating physiological and molecular mechanisms of ethylene induced ripening and low temperature modulated ripening in kiwifruit en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WILLIAM OLUBERO ASICHE en-aut-sei=WILLIAM OLUBERO ASICHE en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=光学活性アルコールを基軸とする香気物質および抗生物質の立体選択的合成研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SakauchiHiroyuki en-aut-sei=Sakauchi en-aut-mei=Hiroyuki kn-aut-name=阪内啓之 kn-aut-sei=阪内 kn-aut-mei=啓之 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=3 article-no= start-page=e33800 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120329 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Practical Application of Methanol-Mediated Mutualistic Symbiosis between Methylobacterium Species and a Roof Greening Moss, Racomitrium japonicum en-subtitle= kn-subtitle= en-abstract= kn-abstract=Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production. en-copyright= kn-copyright= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakaiYuichiro en-aut-sei=Takai en-aut-mei=Yuichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukawaIkko en-aut-sei=Suzukawa en-aut-mei=Ikko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkitaMotomu en-aut-sei=Akita en-aut-mei=Motomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MuraseHaruhiko en-aut-sei=Murase en-aut-mei=Haruhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimbaraKazuhide en-aut-sei=Kimbara en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=2 en-affil= kn-affil=Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government affil-num=3 en-affil= kn-affil=Meiho-Construction affil-num=4 en-affil= kn-affil=Faculty of Biological Engineering, Kinki University affil-num=5 en-affil= kn-affil=Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University affil-num=6 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=7 article-no= start-page=e40784 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120712 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=High-Throughput Identification and Screening of Novel Methylobacterium Species Using Whole-Cell MALDI-TOF/MS Analysis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Methylobacterium species are ubiquitous α-proteobacteria that reside in the phyllosphere and are fed by methanol that is emitted from plants. In this study, we applied whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (WC-MS) to evaluate the diversity of Methylobacterium species collected from a variety of plants. The WC-MS spectrum was reproducible through two weeks of cultivation on different media. WC-MS spectrum peaks of M. extorquens strain AM1 cells were attributed to ribosomal proteins, but those were not were also found. We developed a simple method for rapid identification based on spectra similarity. Using all available type strains of Methylobacterium species, the method provided a certain threshold similarity value for species-level discrimination, although the genus contains some type strains that could not be easily discriminated solely by 16S rRNA gene sequence similarity. Next, we evaluated the WC-MS data of approximately 200 methylotrophs isolated from various plants with MALDI Biotyper software (Bruker Daltonics). Isolates representing each cluster were further identified by 16S rRNA gene sequencing. In most cases, the identification by WC-MS matched that by sequencing, and isolates with unique spectra represented possible novel species. The strains belonging to M. extorquens, M. adhaesivum, M. marchantiae, M. komagatae, M. brachiatum, M. radiotolerans, and novel lineages close to M. adhaesivum, many of which were isolated from bryophytes, were found to be the most frequent phyllospheric colonizers. The WC-MS technique provides emerging high-throughputness in the identification of known/novel species of bacteria, enabling the selection of novel species in a library and identification without 16S rRNA gene sequencing. en-copyright= kn-copyright= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NurettinSahin en-aut-sei=Nurettin en-aut-mei=Sahin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuyamaYumiko en-aut-sei=Matsuyama en-aut-mei=Yumiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=EnomotoTakashi en-aut-sei=Enomoto en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NishimuraNaoki en-aut-sei=Nishimura en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YokotaAkira en-aut-sei=Yokota en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KimbaraKazuhide en-aut-sei=Kimbara en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=2 en-affil= kn-affil=Faculty of Education, Mugla Sitki Kocman University affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=5 en-affil= kn-affil=Botanical Garden, Okayama University of Science affil-num=6 en-affil= kn-affil=Faculty of Mathematics and Natural Sciences, University of Indonesia affil-num=7 en-affil= kn-affil=Faculty of Engineering, Shizuoka University END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=11 article-no= start-page=e50480 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130322 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Catalytic Role of XoxF1 as La3+-Dependent Methanol Dehydrogenase in Methylobacterium extorquens Strain AM1 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca2+-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol. The genome of strain AM1 contains another MDH gene homologue, xoxF1, whose function in methanol metabolism has remained unclear. In this work, we show that XoxF1 also functions as an MDH and is La3+-dependent. Despite the absence of Ca2+ in the medium strain AM1 was able to grow on methanol in the presence of La3+. Addition of La3+ increased MDH activity but the addition had no effect on mxaF or xoxF1 expression level. We purified MDH from strain AM1 grown on methanol in the presence of La3+, and its N-terminal amino acid sequence corresponded to that of XoxF1. The enzyme contained La3+ as a cofactor. The ΔmxaF mutant strain could not grow on methanol in the presence of Ca2+, but was able to grow after supplementation with La3+. Taken together, these results show that XoxF1 participates in methanol metabolism as a La3+-dependent MDH in strain AM1. en-copyright= kn-copyright= en-aut-name=NakagawaTomoyuki en-aut-sei=Nakagawa en-aut-mei=Tomoyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MitsuiRyoji en-aut-sei=Mitsui en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SasaKentaro en-aut-sei=Sasa en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TashiroShinya en-aut-sei=Tashiro en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IwamaTomonori en-aut-sei=Iwama en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HayakawaTakashi en-aut-sei=Hayakawa en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KawaiKeiichi en-aut-sei=Kawai en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Faculty of Applied Biological Science, Gifu University affil-num=2 en-affil= kn-affil=Department of Biochemistry, Faculty of Science, Okayama University of Science affil-num=3 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=4 en-affil= kn-affil=Faculty of Applied Biological Science, Gifu University affil-num=5 en-affil= kn-affil=Faculty of Applied Biological Science, Gifu University affil-num=6 en-affil= kn-affil=Faculty of Applied Biological Science, Gifu University affil-num=7 en-affil= kn-affil=Faculty of Applied Biological Science, Gifu University affil-num=8 en-affil= kn-affil=Tokai Gakuin University END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=15 article-no= start-page=6131 end-page=6141 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150415 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cryptochrome-dependent and -independent circadian entrainment circuits in Drosophila. en-subtitle= kn-subtitle= en-abstract= kn-abstract=　Entrainment to environmental light/dark (LD) cycles is a central function of circadian clocks. In Drosophila, entrainment is achieved by Cryptochrome (CRY) and input from the visual system. During activation by brief light pulses, CRY triggers the degradation of TIMELESS and subsequent shift in circadian phase. This is less important for LD entrainment, leading to questions regarding light input circuits and mechanisms from the visual system. Recent studies show that different subsets of brain pacemaker clock neurons, the morning (M) and evening (E) oscillators, have distinct functions in light entrainment. However, the role of CRY in M and E oscillators for entrainment to LD cycles is unknown. Here, we address this question by selectively expressing CRY in different subsets of clock neurons in a cry-null (cry0) mutant background. We were able to rescue the light entrainment deficits of cry0 mutants by expressing CRY in E oscillators but not in any other clock neurons. Par domain protein 1 molecular oscillations in the E, but not M, cells of cry0 mutants still responded to the LD phase delay. This residual light response was stemming from the visual system because it disappeared when all external photoreceptors were ablated genetically. We concluded that the E oscillators are the targets of light input via CRY and the visual system and are required for normal light entrainment. en-copyright= kn-copyright= en-aut-name=YoshiiTaishi en-aut-sei=Yoshii en-aut-mei=Taishi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Hermann-LuiblChristiane en-aut-sei=Hermann-Luibl en-aut-mei=Christiane kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KistenpfennigChrista en-aut-sei=Kistenpfennig en-aut-mei=Christa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SchmidBenjamin en-aut-sei=Schmid en-aut-mei=Benjamin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TomiokaKenji en-aut-sei=Tomioka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Helfrich-FörsterCharlotte en-aut-sei=Helfrich-Förster en-aut-mei=Charlotte kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg affil-num=3 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Max Planck Institute of Molecular Cell Biology and Genetics affil-num=5 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=6 en-affil= kn-affil=2Neurobiology and Genetics, Theodor-Boveri Institute, Biocenter, University of Würzburg en-keyword=circadian clock kn-keyword=circadian clock en-keyword=clock neurons kn-keyword=clock neurons en-keyword=Cryptochrome kn-keyword=Cryptochrome en-keyword=Drosophila melanogaster kn-keyword=Drosophila melanogaster en-keyword=light entrainment kn-keyword=light entrainment END start-ver=1.4 cd-journal=joma no-vol=142 cd-vols= no-issue=17 article-no= start-page=2916 end-page=2927 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201509 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Leg regeneration is epigenetically regulated by histone H3K27 methylation in the cricket Gryllus bimaculatus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression. en-copyright= kn-copyright= en-aut-name=HamadaYoshimasa en-aut-sei=Hamada en-aut-mei=Yoshimasa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=BandoTetsuya en-aut-sei=Bando en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NakamuraTaro en-aut-sei=Nakamura en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IshimaruYoshiyasu en-aut-sei=Ishimaru en-aut-mei=Yoshiyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MitoTaro en-aut-sei=Mito en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NojiSumihare en-aut-sei=Noji en-aut-mei=Sumihare kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TomiokaKenji en-aut-sei=Tomioka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OhuchiHideyo en-aut-sei=Ohuchi en-aut-mei=Hideyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School affil-num=4 en-affil= kn-affil=Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School affil-num=5 en-affil= kn-affil=Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School affil-num=6 en-affil= kn-affil=Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School affil-num=7 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=8 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=Regeneration kn-keyword=Regeneration en-keyword=Epigenetics kn-keyword=Epigenetics en-keyword=Histone H3K27 kn-keyword=Histone H3K27 en-keyword=Gryllus bimaculatus kn-keyword=Gryllus bimaculatus en-keyword=Polycomb kn-keyword=Polycomb END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=3 article-no= start-page=e91156 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140313 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Genetic Characterization of Hepatitis C Virus in Long-Term RNA Replication Using Li23 Cell Culture Systems en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background　 　 The most distinguishing genetic feature of hepatitis C virus (HCV) is its remarkable diversity and variation. To understand this feature, we previously performed genetic analysis of HCV in the long-term culture of human hepatoma HuH-7-derived HCV RNA-replicating cell lines. On the other hand, we newly established HCV RNA-replicating cell lines using human hepatoma Li23 cells, which were distinct from HuH-7 cells. 　 Methodology/Principal Findings　 　 Li23-derived HCV RNA-replicating cells were cultured for 4 years. We performed genetic analysis of HCVs recovered from these cells at 0, 2, and 4 years in culture. Most analysis was performed in two separate parts: one part covered from the 5′-terminus to NS2, which is mostly nonessential for RNA replication, and the other part covered from NS3 to NS5B, which is essential for RNA replication. Genetic mutations in both regions accumulated in a time-dependent manner, and the mutation rates in the 5′-terminus-NS2 and NS3-NS5B regions were 4.0–9.0×10−3 and 2.7–4.0×10−3 base substitutions/site/year, respectively. These results suggest that the variation in the NS3-NS5B regions is affected by the pressure of RNA replication. Several in-frame deletions (3–105 nucleotides) were detected in the structural regions of HCV RNAs obtained from 2-year or 4-year cultured cells. Phylogenetic tree analyses clearly showed that the genetic diversity of HCV was expanded in a time-dependent manner. The GC content of HCV RNA was significantly increased in a time-dependent manner, as previously observed in HuH-7-derived cell systems. This phenomenon was partially due to the alterations in codon usages for codon optimization in human cells. Furthermore, we demonstrated that these long-term cultured cells were useful as a source for the selection of HCV clones showing resistance to anti-HCV agents. 　 Conclusions/Significance　 　 Long-term cultured HCV RNA-replicating cells are useful for the analysis of evolutionary dynamics and variations of HCV and for drug-resistance analysis. en-copyright= kn-copyright= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SejimaHiroe en-aut-sei=Sejima en-aut-mei=Hiroe kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UedaYouki en-aut-sei=Ueda en-aut-mei=Youki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MoriKyoko en-aut-sei=Mori en-aut-mei=Kyoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SatohShinya en-aut-sei=Satoh en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=DansakoHiromichi en-aut-sei=Dansako en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=8 article-no= start-page=e72519 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=New Preclinical Antimalarial Drugs Potently Inhibit Hepatitis C Virus Genotype 1b RNA Replication en-subtitle= kn-subtitle= en-abstract= kn-abstract=BACKGROUND: Persistent hepatitis C virus (HCV) infection causes chronic liver diseases and is a global health problem. Although new triple therapy (pegylated-interferon, ribavirin, and telaprevir/boceprevir) has recently been started and is expected to achieve a sustained virologic response of more than 70% in HCV genotype 1 patients, there are several problems to be resolved, including skin rash/ageusia and advanced anemia. Thus a new type of anti-HCV drug is still needed. 　 METHODOLOGY/PRINCIPAL FINDINGS: 　 　 Recently developed HCV drug assay systems using HCV-RNA-replicating cells (e.g., HuH-7-derived OR6 and Li23-derived ORL8) were used to evaluate the anti-HCV activity of drug candidates. During the course of the evaluation of anti-HCV candidates, we unexpectedly found that two preclinical antimalarial drugs (N-89 and its derivative N-251) showed potent anti-HCV activities at tens of nanomolar concentrations irrespective of the cell lines and HCV strains of genotype 1b. We confirmed that replication of authentic HCV-RNA was inhibited by these drugs. Interestingly, however, this anti-HCV activity did not work for JFH-1 strain of genotype 2a. We demonstrated that HCV-RNA-replicating cells were cured by treatment with only N-89. A comparative time course assay using N-89 and interferon-α demonstrated that N-89-treated ORL8 cells had more rapid anti-HCV kinetics than did interferon-α-treated cells. This anti-HCV activity was largely canceled by vitamin E. In combination with interferon-α and/or ribavirin, N-89 or N-251 exhibited a synergistic inhibitory effect.　 　 CONCLUSIONS/SIGNIFICANCE:　 　 We found that the preclinical antimalarial drugs N-89 and N-251 exhibited very fast and potent anti-HCV activities using cell-based HCV-RNA-replication assay systems. N-89 and N-251 may be useful as a new type of anti-HCV reagents when used singly or in combination with interferon and/or ribavirin. en-copyright= kn-copyright= en-aut-name=UedaYouki en-aut-sei=Ueda en-aut-mei=Youki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakedaMidori en-aut-sei=Takeda en-aut-mei=Midori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MoriKyoko en-aut-sei=Mori en-aut-mei=Kyoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DansakoHiromichi en-aut-sei=Dansako en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WakitaTakaji en-aut-sei=Wakita en-aut-mei=Takaji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KimHye-Sook en-aut-sei=Kim en-aut-mei=Hye-Sook kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SatoAkira en-aut-sei=Sato en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=WatayaYusuke en-aut-sei=Wataya en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Virology II, National Institute of Infectious Disease affil-num=6 en-affil= kn-affil=Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=7 en-affil= kn-affil=Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=8 en-affil= kn-affil=Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Okayama University affil-num=9 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=10 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences END start-ver=1.4 cd-journal=joma no-vol=127 cd-vols= no-issue=3 article-no= start-page=187 end-page=195 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20151201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Basic and clinical research regarding vascular endothelial function kn-title=血管内皮機能を対象にした基礎および臨床医学研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsukaharaHirokazu en-aut-sei=Tsukahara en-aut-mei=Hirokazu kn-aut-name=塚原宏一 kn-aut-sei=塚原 kn-aut-mei=宏一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 en-keyword=アルギニン代謝 kn-keyword=アルギニン代謝 en-keyword=一酸化窒素 kn-keyword=一酸化窒素 en-keyword=ガス生物学 kn-keyword=ガス生物学 en-keyword=血管内皮学 kn-keyword=血管内皮学 en-keyword=酸化ストレス kn-keyword=酸化ストレス END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=2015 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The cyclic GMP-AMP synthetase-STING signaling pathway is required for both the innate immune response against HBV and the suppression of HBV assembly en-subtitle= kn-subtitle= en-abstract= kn-abstract=During viral replication, the innate immune response is induced through the recognition of viral replication intermediates by host factor(s). One of these host factors, cyclic GMP-AMP synthetase (cGAS), was recently reported to be involved in the recognition of viral DNA derived from DNA viruses. However, it is uncertain whether cGAS is involved in the recognition of hepatitis B virus (HBV), which is a hepatotropic DNA virus. In the present study, we demonstrated that HBV genome-derived dsDNA induced the innate immune response through cGAS and its adaptor protein, STING, in human hepatoma Li23 cells expressing high levels of cGAS. In addition, we demonstrated that HBV infection induced ISG56 through the cGAS-STING signaling pathway. This signaling pathway also showed an antiviral response towards HBV through the suppression of viral assembly. From these results, we conclude that the cGAS-STING signaling pathway is required for not only the innate immune response against HBV but also the suppression of HBV assembly. The cGAS-STING signaling pathway may thus be a novel target for anti-HBV strategies. en-copyright= kn-copyright= en-aut-name=DansakoHiromichi en-aut-sei=Dansako en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UedaYouki en-aut-sei=Ueda en-aut-mei=Youki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkumuraNobuaki en-aut-sei=Okumura en-aut-mei=Nobuaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SatohShinya en-aut-sei=Satoh en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SugiyamaMasaya en-aut-sei=Sugiyama en-aut-mei=Masaya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MizokamiMasashi en-aut-sei=Mizokami en-aut-mei=Masashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine affil-num=6 en-affil= kn-affil=Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine affil-num=7 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry affil-num=8 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences en-keyword=Antiviral response kn-keyword=Antiviral response en-keyword=hepatitis B virus kn-keyword=hepatitis B virus en-keyword=innate immune response kn-keyword=innate immune response en-keyword=cGAS-STING signaling pathway kn-keyword=cGAS-STING signaling pathway en-keyword=viral assembly kn-keyword=viral assembly END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue= article-no= start-page=606 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20131113 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Evaluation of rhizosphere, rhizoplane and phyllosphere bacteria and fungi isolated from rice in Kenya for plant growth promoters en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rice (Oryza sativa L.) is the most important staple food crop in many developing countries, and is ranked third in Kenya after maize and wheat. Continuous cropping without replenishing soil nutrients is a major problem in Kenya resulting to declining soil fertility. The use of chemical fertilizers to avert the problem of low soil fertility is currently limited due to rising costs and environmental concerns. Many soil micro-organisms are able to solubilize the unavailable phosphorus, increase uptake of nitrogen and also synthesize growth promoting hormones including auxin. The aim of this study was to isolate and characterize phyllosphere, rhizoplane and rhizosphere micro-organisms from Kenyan rice with growth promoting habits. In this study whole plant rice samples were collected from different rice growing regions of Kenya. 76.2%, over 80% and 38.5% of the bacterial isolates were positive for phosphate solubilization, nitrogenase activity and IAA production whereas 17.5% and 5% of the fungal isolates were positive for phosphate solubilization and IAA production respectively. Hence these micro-organisms have potential for utilization as bio-fertilizers in rice production. en-copyright= kn-copyright= en-aut-name=Rashid MwajitaMwashasha en-aut-sei=Rashid Mwajita en-aut-mei=Mwashasha kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MurageHunja en-aut-sei=Murage en-aut-mei=Hunja kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaniAkio en-aut-sei=Tani en-aut-mei=Akio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KahangiEsther M en-aut-sei=Kahangi en-aut-mei=Esther M kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Kenya Agricultural Research Institute affil-num=2 en-affil= kn-affil=Jomo Kenyatta University of Agriculture and Technology affil-num=3 en-affil= kn-affil=Institute of Plant Science and Resources Okayama University affil-num=4 en-affil= kn-affil=Jomo Kenyatta University of Agriculture and Technology en-keyword=Micro-organisms kn-keyword=Micro-organisms en-keyword=Phosphate solubilization kn-keyword=Phosphate solubilization en-keyword=Nitrogen fixation kn-keyword=Nitrogen fixation en-keyword=IAA production kn-keyword=IAA production END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=培養法および非培養法によるアルファルファサイレージの嫌気的貯蔵および好気的変敗に関わる微生物フローラの解析 kn-title=Culture-dependent and culture-independent analysis of microbiota associated with anaerobic storage and aerobic deterioration of alfalfa silage en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WuBaiyila en-aut-sei=Wu en-aut-mei=Baiyila kn-aut-name=呉白乙拉 kn-aut-sei=呉 kn-aut-mei=白乙拉 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院 END start-ver=1.4 cd-journal=joma no-vol=69 cd-vols= no-issue=2 article-no= start-page=71 end-page=78 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201504 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Annexin A1 Negatively Regulates Viral RNA Replication of Hepatitis C Virus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Persistent infection with hepatitis C virus (HCV) often causes chronic hepatitis, and then shows a high rate of progression to liver cirrhosis and hepatocellular carcinoma. To clarify the mechanism of the persistent HCV infection is considered to be important for the discovery of new target(s) for the development of anti-HCV strategies. In the present study, we found that the expression level of annexin A1 (ANXA1) in human hepatoma cell line Li23-derived D7 cells was remarkably lower than that in parental Li23 cells, whereas the susceptibility of D7 cells to HCV infection was much higher than that of Li23 cells. Therefore, we hypothesized that ANXA1 negatively regulates persistent HCV infection through the inhibition of viral RNA replication. The results revealed that HCV production was significantly inhibited without a concomitant reduction in the amount of lipid droplets in the D7 cells stably expressing exogenous ANXA1. Further, we demonstrated that ANXA1 negatively regulated the step of viral RNA replication rather than that of viral entry in human hepatocytes. These results suggest that ANXA1 would be a novel target for the development of anti-HCV strategies. en-copyright= kn-copyright= en-aut-name=HiramotoHiroki en-aut-sei=Hiramoto en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DansakoHiromichi en-aut-sei=Dansako en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaMidori en-aut-sei=Takeda en-aut-mei=Midori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SatohShinya en-aut-sei=Satoh en-aut-mei=Shinya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WakitaTakaji en-aut-sei=Wakita en-aut-mei=Takaji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IkedaMasanori en-aut-sei=Ikeda en-aut-mei=Masanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KatoNobuyuki en-aut-sei=Kato en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Virology II, National Institute of Infectious Disease affil-num=6 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Tumor Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=HCV kn-keyword=HCV en-keyword=annexin A1 kn-keyword=annexin A1 en-keyword=Li23 cell line kn-keyword=Li23 cell line en-keyword=Li23-derived D7 cells kn-keyword=Li23-derived D7 cells en-keyword=HCV-JFH-1 kn-keyword=HCV-JFH-1 END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue= article-no= start-page=1 end-page=2 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=開発と生物多様性保全 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=山本進一 kn-aut-sei=山本 kn-aut-mei=進一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 理事・副学長 END start-ver=1.4 cd-journal=joma no-vol=2013 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Bulletin of Archaelogical Research Center Okayama University 2013 kn-title=岡山大学埋蔵文化財調査研究センター紀要2013 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=野崎貴博 kn-aut-sei=野崎 kn-aut-mei=貴博 aut-affil-num=1 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=南健太郎 kn-aut-sei=南 kn-aut-mei=健太郎 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岩崎志保 kn-aut-sei=岩崎 kn-aut-mei=志保 aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=吉田生物研究所 kn-aut-sei=吉田生物研究所 kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=甲元眞之 kn-aut-sei=甲元 kn-aut-mei=眞之 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil= affil-num=5 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=関節リウマチ患者のMRI評価： 生物学的製剤投与患者評価におけるOMERACT (Outcome Measures in Rheumatology Clinical Trials) スコアシステム評価と滑膜体積の比較 kn-title=MRI of Rheumatoid Arthritis: Comparing the Outcome Measures in Rheumatology Clinical Trials (OMERACT) Scoring and Volume of Synovitis for the Assessment of Biologic Therapy en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakaharaRyuichi en-aut-sei=Nakahara en-aut-mei=Ryuichi kn-aut-name=中原龍一 kn-aut-sei=中原 kn-aut-mei=龍一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue= article-no= start-page=35 end-page=47 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Microbiological Research on the Traditional Alcoholic Fermented Milk“Airag”in Mongolia kn-title=モンゴルの伝統的アルコール発酵乳アイラグに関する微生物学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　Airag, a traditional fermented milk well loved by Mongolians, has been drunk habitually since ancient times as a unique drink. It is produced from cow, mare and camel milk by a traditional method using indigenous starter cultures containing lactic acid bacteria, yeasts and other fermentative microorganisms. Spontaneously fermented milk products have for centuries been consumed for their therapeutic value in promoting health and well-being, especially among the pastoral communities in Mongolia. Most traditional fermentations are conducted as uncontrolled processes, however, increasing research, such as that focused on compositional properties and microbial biota properties, offers prospective views for improving spontaneous fermented products with respect to safety, shelf life, sensory characteristics, and nutritional and functional qualities. The aim of this article is to investigate progress in the microbiological research on the traditional alcoholic fermented milk, airag, in Mongolia and to give an outline of the traditional preparation processes. en-copyright= kn-copyright= en-aut-name=MiyamotoTaku en-aut-sei=Miyamoto en-aut-mei=Taku kn-aut-name=宮本拓 kn-aut-sei=宮本 kn-aut-mei=拓 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学農学部 en-keyword=airag kn-keyword=airag en-keyword=alcoholic fermented milk kn-keyword=alcoholic fermented milk en-keyword=lactic acid bacteria kn-keyword=lactic acid bacteria en-keyword=yeasts kn-keyword=yeasts en-keyword=traditional preparation process kn-keyword=traditional preparation process END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue= article-no= start-page=5 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=20150201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=好酸性鉄酸化細菌 Acidithiobacillus ferrooxidans の染色体凝集・分配タンパク質（ScpB）の性質 kn-title=Characterization of a putative chromosome segregation and condensation protein (ScpB) in an acidophilic iron‒oxidizing bacterium Acidithiobacillus ferrooxidans en-subtitle= kn-subtitle= en-abstract=　Acidithiobacillus ferrooxidans は，低品位の銅鉱石から銅を回収するバイオリーチングにおいて使用される微生物 の一つである．この細菌は，エネルギー源として二価鉄イオンや還元型無機硫黄化合物（RISC）を使用する．鉄の酸化に関与する遺伝子の転写は，A. ferrooxidans が RISC で生育したときには抑制されるが，RISC の酸化に関与すると考えられている遺伝子の転写は活性化されることが知られている．硫黄やテトラチオン酸で生育したときにその発現が上方制御される硫化水素：キノン酸化還元酵素のすぐ上流に，ヘリックスターンへリックスモティーフを持つ，ScpB と推定されるタンパク質をコードする遺伝子が存在していた．鉄，硫黄，テトラチオン酸生育細胞から調製した cDNA を用いた半定量的 PCR 分析の結果，硫黄やテトラチオン酸で生育した細胞内の scpB 遺伝子の転写は，鉄生育細胞と比較すると上方制御されていた．組換え ScpB タンパク質を用いたゲルシフトアッセイ法で，ScpB が転写制御因子として機能するかどうかを調べた．その結果，ScpB は DNA に結合したが，結合の特異性はなかった．ScpB が直接 DNA と相互作用する報告はこれまでになかった． kn-abstract=　Acidithiobacillus ferrooxidans is one of the most widely used microorganisms in bioleaching operations to recover copper from low-grade copper sulfide. This bacterium uses ferrous iron and reduced inorganic sulfur compounds (RISCs) as energy sources. Transcriptions of genes thought to be involved in the oxidation of RISCs have been known to be highly activated in A. ferrooxidans cells grown on RISCs, while transcriptions of genes involved in the iron oxidation were repressed in the cells grown on RISCs. A gene encoding a putative chromosome segregation and condensation protein (ScpB) with a helix-turn-helix motif was found in the upstream region of sulfide : quinone oxidoreductase gene, whose expression was up-regulated in cells grown in sulfur and tetrathionate. A semi-quantitative PCR analysis using cDNA prepared from iron-, sulfur-, or tetrathionate-grown cells revealed that the transcription of scpB was up-regulated in cells grown on sulfur or tetrathionate as the energy source. Electrophoretic mobility shift assays were employed to examine whether the ScpB functions as a transcription factor. The result indicated that the recombinant His-tagged ScpB protein was able to nonspecifically bind in vitro to DNA. This is the first report on a direct association of ScpB with DNA. en-copyright= kn-copyright= en-aut-name=KamimuraKazuo en-aut-sei=Kamimura en-aut-mei=Kazuo kn-aut-name=上村一雄 kn-aut-sei=上村 kn-aut-mei=一雄 aut-affil-num=1 ORCID= en-aut-name=NagataNozomu en-aut-sei=Nagata en-aut-mei=Nozomu kn-aut-name=長田臨 kn-aut-sei=長田 kn-aut-mei=臨 aut-affil-num=2 ORCID= en-aut-name=KikumotoMei en-aut-sei=Kikumoto en-aut-mei=Mei kn-aut-name=菊本愛生 kn-aut-sei=菊本 kn-aut-mei=愛生 aut-affil-num=3 ORCID= en-aut-name=SharminSultana en-aut-sei=Sharmin en-aut-mei=Sultana kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WakaiSatoshi en-aut-sei=Wakai en-aut-mei=Satoshi kn-aut-name=若井暁 kn-aut-sei=若井 kn-aut-mei=暁 aut-affil-num=5 ORCID= en-aut-name=KanaoTadayoshi en-aut-sei=Kanao en-aut-mei=Tadayoshi kn-aut-name=金尾忠芳 kn-aut-sei=金尾 kn-aut-mei=忠芳 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院環境生命科学研究科 affil-num=2 en-affil= kn-affil=岡山大学大学院環境生命科学研究科 affil-num=3 en-affil= kn-affil=岡山大学大学院環境生命科学研究科 affil-num=4 en-affil= kn-affil=岡山大学大学院環境生命科学研究科 affil-num=5 en-affil= kn-affil=神戸大学自然科学系先端融合研究環 affil-num=6 en-affil= kn-affil=神戸大学自然科学系先端融合研究環 en-keyword=Acidithiobacillus ferrooxidans kn-keyword=Acidithiobacillus ferrooxidans en-keyword=Acidophile kn-keyword=Acidophile en-keyword=ScpB kn-keyword=ScpB en-keyword=transcription factor kn-keyword=transcription factor END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=12 article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141211 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Nuclear Receptor Genes HR3 and E75 Are Required for the Circadian Rhythm in a Primitive Insect en-subtitle= kn-subtitle= en-abstract= kn-abstract=Insect circadian rhythms are generated by a circadian clock consisting of transcriptional/translational feedback loops, in which CYCLE and CLOCK are the key elements in activating the transcription of various clock genes such as timeless (tim) and period (per). Although the transcriptional regulation of Clock (Clk) has been profoundly studied, little is known about the regulation of cycle (cyc). Here, we identify the orphan nuclear receptor genes HR3 and E75, which are orthologs of mammalian clock genes, Rorα and Rev-erbα, respectively, as factors involved in the rhythmic expression of the cyc gene in a primitive insect, the firebrat Thermobia domestica. Our results show that HR3 and E75 are rhythmically expressed, and their normal, rhythmic expression is required for the persistence of locomotor rhythms. Their RNAi considerably altered the rhythmic transcription of not only cyc but also tim. Surprisingly, the RNAi of HR3 revealed the rhythmic expression of Clk, suggesting that this ancestral insect species possesses the mechanisms for rhythmic expression of both cyc and Clk genes. When either HR3 or E75 was knocked down, tim, cyc, and Clk or tim and cyc, respectively, oscillated in phase, suggesting that the two genes play an important role in the regulation of the phase relationship among the clock genes. Interestingly, HR3 and E75 were also found to be involved in the regulation of ecdysis, suggesting that they interconnect the circadian clock and developmental processes. en-copyright= kn-copyright= en-aut-name=KamaeYuichi en-aut-sei=Kamae en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UryuOuta en-aut-sei=Uryu en-aut-mei=Outa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MikiTaiki en-aut-sei=Miki en-aut-mei=Taiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TomiokaKenji en-aut-sei=Tomioka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Biology, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University END start-ver=1.4 cd-journal=joma no-vol=126 cd-vols= no-issue=3 article-no= start-page=227 end-page=230 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Molecular target therapies in rheumatic diseases kn-title=リウマチ性疾患における分子標的治療 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WakabayashiHiroshi en-aut-sei=Wakabayashi en-aut-mei=Hiroshi kn-aut-name=若林宏 kn-aut-sei=若林 kn-aut-mei=宏 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学 en-keyword=リウマチ性疾患 kn-keyword=リウマチ性疾患 en-keyword=自己免疫疾患 kn-keyword=自己免疫疾患 en-keyword=生物学的製剤 kn-keyword=生物学的製剤 en-keyword=分子標的薬 kn-keyword=分子標的薬 END start-ver=1.4 cd-journal=joma no-vol=126 cd-vols= no-issue=3 article-no= start-page=209 end-page=215 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20141201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hospital and clinic cooperation for the treatment of rheumatoid arthritis in Okayama Prefecture, Japan kn-title=岡山県下のクリニック・診療所におけるリウマチ診療・病診連携の実態に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objective: To survey the current status and problems of cooperation between clinics and hospitals in Okayama Prefecture, Japan for the treatment of rheumatoid arthritis (RA). 　Methods: We distributed a questionnaire to 300 of the 983 Okayama Prefecture clinics that had either an internal medicine or orthopedic surgery department, from December 2013 to February 2014. The questionnaire covered practice pattern for RA treatment in clinics, current status of the hospital and clinic cooperation, and acceptance of the biologic therapy. 　Results: One hundred clinics responded to the questionnaire. Seventy percent of the clinics reported making referrals to rheumatologists before the initiation of RA treatment, and half of the other 30% of the clinics administered methotrexate as the first-line treatment for RA by their own decision. Sixty-six clinics cooperated with flagship hospitals, conducting medical and laboratory examinations, providing prescriptions, and treating common diseases of patients. These clinics expected the cooperating rheumatologists to follow-up patients every 3 to 6 months and to make the diagnosis, make decisions regarding RA treatment changes, and perform surgery. Seventy-one percent of the clinics responded that cooperation with a hospital is possible even for patients who are administered biologics. As reasons for no cooperation with the flagship hospitals, clinics noted the lack of information about rheumatologists in the area and recent trends in the management of RA. 　Conclusion: The current study reported, for the first time, the actual conditions of management of RA in clinics, as well as future problems of hospital and clinic cooperation in Okayama Prefecture. en-copyright= kn-copyright= en-aut-name=SadaKen-ei en-aut-sei=Sada en-aut-mei=Ken-ei kn-aut-name=佐田憲映 kn-aut-sei=佐田 kn-aut-mei=憲映 aut-affil-num=1 ORCID= en-aut-name=NishidaKeiichiro en-aut-sei=Nishida en-aut-mei=Keiichiro kn-aut-name=西田圭一郎 kn-aut-sei=西田 kn-aut-mei=圭一郎 aut-affil-num=2 ORCID= en-aut-name=YamanakaTakao en-aut-sei=Yamanaka en-aut-mei=Takao kn-aut-name=山中隆夫 kn-aut-sei=山中 kn-aut-mei=隆夫 aut-affil-num=3 ORCID= en-aut-name=MisakiKenta en-aut-sei=Misaki en-aut-mei=Kenta kn-aut-name=三崎健太 kn-aut-sei=三崎 kn-aut-mei=健太 aut-affil-num=4 ORCID= en-aut-name=WakabayashiHiroshi en-aut-sei=Wakabayashi en-aut-mei=Hiroshi kn-aut-name=若林宏 kn-aut-sei=若林 kn-aut-mei=宏 aut-affil-num=5 ORCID= en-aut-name=ShinodaJunko en-aut-sei=Shinoda en-aut-mei=Junko kn-aut-name=篠田潤子 kn-aut-sei=篠田 kn-aut-mei=潤子 aut-affil-num=6 ORCID= en-aut-name=TakagiToru en-aut-sei=Takagi en-aut-mei=Toru kn-aut-name=髙木徹 kn-aut-sei=髙木 kn-aut-mei=徹 aut-affil-num=7 ORCID= en-aut-name=YanoRyusuke en-aut-sei=Yano en-aut-mei=Ryusuke kn-aut-name=矢野隆介 kn-aut-sei=矢野 kn-aut-mei=隆介 aut-affil-num=8 ORCID= en-aut-name=NakamuraAkihiko en-aut-sei=Nakamura en-aut-mei=Akihiko kn-aut-name=中村明彦 kn-aut-sei=中村 kn-aut-mei=明彦 aut-affil-num=9 ORCID= en-aut-name=NanbaYoshifumi en-aut-sei=Nanba en-aut-mei=Yoshifumi kn-aut-name=難波良文 kn-aut-sei=難波 kn-aut-mei=良文 aut-affil-num=10 ORCID= en-aut-name=MoritaYoshitaka en-aut-sei=Morita en-aut-mei=Yoshitaka kn-aut-name=守田吉孝 kn-aut-sei=守田 kn-aut-mei=吉孝 aut-affil-num=11 ORCID= en-aut-name=KoyamaYoshinobu en-aut-sei=Koyama en-aut-mei=Yoshinobu kn-aut-name=小山芳伸 kn-aut-sei=小山 kn-aut-mei=芳伸 aut-affil-num=12 ORCID= en-aut-name=YamamotoKeiji en-aut-sei=Yamamoto en-aut-mei=Keiji kn-aut-name=山本惠嗣 kn-aut-sei=山本 kn-aut-mei=惠嗣 aut-affil-num=13 ORCID= en-aut-name=EzawaKazuhiko en-aut-sei=Ezawa en-aut-mei=Kazuhiko kn-aut-name=江澤和彦 kn-aut-sei=江澤 kn-aut-mei=和彦 aut-affil-num=14 ORCID= en-aut-name=OtaYusuke en-aut-sei=Ota en-aut-mei=Yusuke kn-aut-name=太田裕介 kn-aut-sei=太田 kn-aut-mei=裕介 aut-affil-num=15 ORCID= en-aut-name=YoshiharaYoshiki en-aut-sei=Yoshihara en-aut-mei=Yoshiki kn-aut-name=吉原由樹 kn-aut-sei=吉原 kn-aut-mei=由樹 aut-affil-num=16 ORCID= en-aut-name=MiyoshiShinya en-aut-sei=Miyoshi en-aut-mei=Shinya kn-aut-name=三好信也 kn-aut-sei=三好 kn-aut-mei=信也 aut-affil-num=17 ORCID= en-aut-name=NatsumedaMasamitsu en-aut-sei=Natsumeda en-aut-mei=Masamitsu kn-aut-name=棗田将光 kn-aut-sei=棗田 kn-aut-mei=将光 aut-affil-num=18 ORCID= en-aut-name=UsuiMasaaki en-aut-sei=Usui en-aut-mei=Masaaki kn-aut-name=臼井正明 kn-aut-sei=臼井 kn-aut-mei=正明 aut-affil-num=19 ORCID= en-aut-name=YoshinagaYasuhiko en-aut-sei=Yoshinaga en-aut-mei=Yasuhiko kn-aut-name=吉永泰彦 kn-aut-sei=吉永 kn-aut-mei=泰彦 aut-affil-num=20 ORCID= en-aut-name=HayashiTakashi en-aut-sei=Hayashi en-aut-mei=Takashi kn-aut-name=林充 kn-aut-sei=林 kn-aut-mei=充 aut-affil-num=21 ORCID= en-aut-name=YamamuraMasahiro en-aut-sei=Yamamura en-aut-mei=Masahiro kn-aut-name=山村昌弘 kn-aut-sei=山村 kn-aut-mei=昌弘 aut-affil-num=22 ORCID= en-aut-name=HashizumeHiroyuki en-aut-sei=Hashizume en-aut-mei=Hiroyuki kn-aut-name=橋詰博行 kn-aut-sei=橋詰 kn-aut-mei=博行 aut-affil-num=23 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　人体構成学 affil-num=3 en-affil= kn-affil=南岡山医療センター　リウマチ科 affil-num=4 en-affil= kn-affil=倉敷中央病院　内分泌代謝・リウマチ内科 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　腎・免疫・内分泌代謝内科学 affil-num=6 en-affil= kn-affil=岡山労災病院　整形外科 affil-num=7 en-affil= kn-affil=岡山赤十字病院　整形外科 affil-num=8 en-affil= kn-affil=矢野内科医院 affil-num=9 en-affil= kn-affil=おさふねクリニック affil-num=10 en-affil= kn-affil=川崎医科大学附属病院　整形外科 affil-num=11 en-affil= kn-affil=川崎医科大学附属病院　リウマチ・膠原病科 affil-num=12 en-affil= kn-affil=岡山赤十字病院　膠原病リウマチ内科 affil-num=13 en-affil= kn-affil=山本整形外科医院 affil-num=14 en-affil= kn-affil=倉敷スイートホスピタル　内科 affil-num=15 en-affil= kn-affil=香川労災病院　整形外科 affil-num=16 en-affil= kn-affil=倉敷成人病センター　整形外科 affil-num=17 en-affil= kn-affil=倉敷成人病センター　整形外科 affil-num=18 en-affil= kn-affil=倉敷スイートホスピタル　内科 affil-num=19 en-affil= kn-affil=岡山市民病院　整形外科 affil-num=20 en-affil= kn-affil=倉敷成人病センター　リウマチ膠原病内科 affil-num=21 en-affil= kn-affil=佐用中央病院　整形外科 affil-num=22 en-affil= kn-affil=岡山済生会総合病院　内科 affil-num=23 en-affil= kn-affil=笠岡第一病院　整形外科 en-keyword=病診連携（hospital and clinic cooperation） kn-keyword=病診連携（hospital and clinic cooperation） en-keyword=関節リウマチ（rheumatoid arthritis） kn-keyword=関節リウマチ（rheumatoid arthritis） en-keyword=生物学的製剤（biologics） kn-keyword=生物学的製剤（biologics） en-keyword=メトトレキサート（methotrexate） kn-keyword=メトトレキサート（methotrexate） END start-ver=1.4 cd-journal=joma no-vol=120 cd-vols= no-issue= article-no= start-page=299 end-page=304 dt-received= dt-revised= dt-accepted= dt-pub-year=2015 dt-pub=201502 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Toxicity of tetramethylammonium hydroxide to aquatic organisms and its synergistic action with potassium iodide en-subtitle= kn-subtitle= en-abstract= kn-abstract=The aquatic ecotoxicity of chemicals involved in the manufacturing process of thin film transistor liquid crystal displays was assessed with a battery of four selected acute toxicity bioassays. We focused on tetramethylammonium hydroxide (TMAH, CAS No. 75-59-2), a widely utilized etchant. The toxicity of TMAH was low when tested in the 72 h-algal growth inhibition test (Pseudokirchneriellia subcapitata, EC50 = 360 mg L−1) and the Microtox® test (Vibrio fischeri, IC50 = 6.4 g L−1). In contrast, the 24 h-microcrustacean immobilization and the 96 h-fish mortality tests showed relatively higher toxicity (Daphnia magna, EC50 = 32 mg L−1 and Oryzias latipes, LC50 = 154 mg L−1). Isobologram and mixture toxicity index analyses revealed apparent synergism of the mixture of TMAH and potassium iodide when examined with the D. magna immobilization test. The synergistic action was unique to iodide over other halide salts i.e. fluoride, chloride and bromide. Quaternary ammonium ions with longer alkyl chains such as tetraethylammonium and tetrabutylammonium were more toxic than TMAH in the D. magna immobilization test. en-copyright= kn-copyright= en-aut-name=MoriIzumi C. en-aut-sei=Mori en-aut-mei=Izumi C. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Arias-BarreiroCarlos R. en-aut-sei=Arias-Barreiro en-aut-mei=Carlos R. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KoutsaftisApostolos en-aut-sei=Koutsaftis en-aut-mei=Apostolos kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OgoAtsushi en-aut-sei=Ogo en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KawanoTomonori en-aut-sei=Kawano en-aut-mei=Tomonori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshizukaKazuharu en-aut-sei=Yoshizuka en-aut-mei=Kazuharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=Inayat-HussainSalmaan H. en-aut-sei=Inayat-Hussain en-aut-mei=Salmaan H. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=AoyamaIsao en-aut-sei=Aoyama en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=2 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=3 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=4 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University affil-num=5 en-affil= kn-affil=School of International Environmental Science, The University of Kitakyushu affil-num=6 en-affil= kn-affil=School of International Environmental Science, The University of Kitakyushu affil-num=7 en-affil= kn-affil=Faculty of Health Sciences, Univerisiti Kebangsaan Malaysia affil-num=8 en-affil= kn-affil=Institute of Plant Science and Resources, Okayama University en-keyword=Tetramethylammonium hydroxide kn-keyword=Tetramethylammonium hydroxide en-keyword=Potassium iodide kn-keyword=Potassium iodide en-keyword=Aquatic toxicity kn-keyword=Aquatic toxicity en-keyword=Synergism kn-keyword=Synergism en-keyword=D. magna kn-keyword=D. magna en-keyword=Semiconductor wastewater kn-keyword=Semiconductor wastewater END start-ver=1.4 cd-journal=joma no-vol=126 cd-vols= no-issue=2 article-no= start-page=127 end-page=131 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fermented persimmon extract (kaki-shibu) is useful as a standard for component analyses of persimmon phytobezoars kn-title=柿胃石の成分分析における標準物質としての柿渋の有用性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The definite diagnosis of persimmon phytobezoar (i.e., diospyrobezoar) is often accomplished by a component analysis using infrared spectroscopy. However, no studies have been conducted to investigate which substance is the best as a standard for the component analysis. Here we analyzed tannic acid, Japanese persimmon (kaki), fermented persimmon extract (kaki-shibu), conventional dried persimmon, and dried persimmon smoked in sulfur (ampo-kaki) by infrared spectroscopy to determine which would be optimal as a component analysis standard. The spectrum between 1,600 to 600cm－1 of a persimmon phytobezoar was quite similar to the spectrum of kaki-shibu rather than that of tannic acid. Consequently, we conclude that kaki-shibu should be used as a standard for infrared spectroscopy analyses of persimmon phytobezoars. en-copyright= kn-copyright= en-aut-name=IwamuroMasaya en-aut-sei=Iwamuro en-aut-mei=Masaya kn-aut-name=岩室雅也 kn-aut-sei=岩室 kn-aut-mei=雅也 aut-affil-num=1 ORCID= en-aut-name=OkamotoYuko en-aut-sei=Okamoto en-aut-mei=Yuko kn-aut-name=岡本裕子 kn-aut-sei=岡本 kn-aut-mei=裕子 aut-affil-num=2 ORCID= en-aut-name=MurataToshihiro en-aut-sei=Murata en-aut-mei=Toshihiro kn-aut-name=村田年弘 kn-aut-sei=村田 kn-aut-mei=年弘 aut-affil-num=3 ORCID= en-aut-name=KawaiYoshinari en-aut-sei=Kawai en-aut-mei=Yoshinari kn-aut-name=河合良成 kn-aut-sei=河合 kn-aut-mei=良成 aut-affil-num=4 ORCID= en-aut-name=ShirahaHidenori en-aut-sei=Shiraha en-aut-mei=Hidenori kn-aut-name=白羽英則 kn-aut-sei=白羽 kn-aut-mei=英則 aut-affil-num=5 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name=岡田裕之 kn-aut-sei=岡田 kn-aut-mei=裕之 aut-affil-num=6 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name=山本和秀 kn-aut-sei=山本 kn-aut-mei=和秀 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 消化器・肝臓内科学 affil-num=2 en-affil= kn-affil=井原市立井原市民病院　内科 affil-num=3 en-affil= kn-affil=尾道市立市民病院 外科 affil-num=4 en-affil= kn-affil=尾道市立市民病院 消化器内科 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 消化器・肝臓内科学 affil-num=6 en-affil= kn-affil=岡山大学病院　光学医療診療部 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 消化器・肝臓内科学 en-keyword=柿胃石（gastric phytobezoar） kn-keyword=柿胃石（gastric phytobezoar） en-keyword=タンニン酸（tannic acid） kn-keyword=タンニン酸（tannic acid） en-keyword=消化管異物（gastrointestinal foreign body） kn-keyword=消化管異物（gastrointestinal foreign body） en-keyword=成分分析（component analysis） kn-keyword=成分分析（component analysis） END start-ver=1.4 cd-journal=joma no-vol=126 cd-vols= no-issue=2 article-no= start-page=83 end-page=86 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The 2013 Incentive Award of the Okayama Medical Association in General Medical Science (2013 Yuuki Prize) kn-title=平成25年度岡山医学会賞 総合研究奨励賞（結城賞） en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MurataHitoshi en-aut-sei=Murata en-aut-mei=Hitoshi kn-aut-name=村田等 kn-aut-sei=村田 kn-aut-mei=等 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　細胞生物学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=199507 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=80年の歴史から21世紀へ向けて : 研究活動と学術的・社会的貢献 : 岡山大学資源生物科学研究所創設80周年記念 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=資源生物科学研究所年報No.3 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=資源生物科学研究所年報No.2 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=資源生物科学研究所年報No.1 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=17 cd-vols= no-issue= article-no= start-page=1 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Annual report 2009 / Research Institute for Bioresources Okayama University kn-title=岡山大学資源生物科学研究所報告17巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Research Institute for Bioresources Okayama University en-aut-sei=Research Institute for Bioresources Okayama University en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=16 cd-vols= no-issue= article-no= start-page=1 end-page=47 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Annual Report 2008 / Research Institute for Bioresources, Okayama University kn-title=岡山大学資源生物科学研究所報告16巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Research Institute for Bioresources, Okayama University en-aut-sei=Research Institute for Bioresources, Okayama University en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue= article-no= start-page=1 end-page=43 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Annual Report 2007 / Research Institute for Bioresources, Okayama University kn-title=岡山大学資源生物科学研究所報告15巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Research Institute for Bioresources, Okayama University en-aut-sei=Research Institute for Bioresources, Okayama University en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=14 cd-vols= no-issue= article-no= start-page=1 end-page=50 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Annual Report 2006 / Research Institute for Bioresources, Okayama University kn-title=岡山大学資源生物科学研究所報告14巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Research Institute for Bioresources, Okayama University en-aut-sei=Research Institute for Bioresources, Okayama University en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=13 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=2005 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告13巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Research Institute for Bioresources, Okayama University en-aut-sei=Research Institute for Bioresources, Okayama University en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=2004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告12巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=11 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=2003 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告11巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=2002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告10巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=2001 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告9巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=2000 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告8巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=1999 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=岡山大学資源生物科学研究所報告7巻 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡山大学資源生物科学研究所 kn-aut-sei=岡山大学資源生物科学研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=新規マイコウイルスRosellinia necatrix megabirnavirus 1:　生物学的および分子生物学的性状，遺伝子発現戦略，ゲノム再編成 kn-title=A novel mycovirus Rosellinia necatrix megabirnavirus 1: Biological and molecular characterization, gene expression strategy, and genome rearrangements en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Lakha Salaipeth en-aut-sei=Lakha Salaipeth en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=中等度酸性鉱山廃水中の微生物叢の解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=王揚 kn-aut-sei=王 kn-aut-mei=揚 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=フタホシコオロギ概日時計機構の分子生物学的研究 kn-title=Molecular analysis of the circadian clock system in the cricket, Gryllus bimaculatus en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=UryuOta en-aut-sei=Uryu en-aut-mei=Ota kn-aut-name=瓜生央大 kn-aut-sei=瓜生 kn-aut-mei=央大 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=口蓋粘膜上皮におけるタイトジャンクションの細胞生物学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ShiotsuNoriko en-aut-sei=Shiotsu en-aut-mei=Noriko kn-aut-name=塩津範子 kn-aut-sei=塩津 kn-aut-mei=範子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=咬合支持の回復による海馬の記憶関連遺伝子発現に関する分子生物学的解析 kn-title=Memory-related gene expression profile of the male rat hippocampus induced by teeth extraction and occlusal support recovery en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=IidaSachiyo en-aut-sei=Iida en-aut-mei=Sachiyo kn-aut-name=飯田祥与 kn-aut-sei=飯田 kn-aut-mei=祥与 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=30 cd-vols= no-issue= article-no= start-page=3 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=201404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The role of autophagy of early embryonic development in mice kn-title=マウス初期胚発生におけるオートファジーの役割 en-subtitle= kn-subtitle= en-abstract= kn-abstract=After fertilization, maternal products which are stored during oogenesis are rapidly degraded and newly zygotic products are synthesized in most animals. This transition, known as an oocyte-to-embryo transition, is critical step for further embryonic development. Considering that the process occurs quickly, bulk degradation system could be highly activated to eliminate maternal products and recycle them to synthesize newly products. We have demonstrated that autophagy, which is cytoplasmic bulk degradation system mediated by the lysosome, is highly induced after fertilization, and autophagy-deficient embryo dies before implantation, suggesting that autophagy is essential for preimplantaion embryonic development. Our recent studies also revealed that lysosomal size and number changes during the embryonic development. Here we will briefly review the autophagy, and discuss the function of autophagy and lysosome in early mouse embryogenesis. en-copyright= kn-copyright= en-aut-name=TsukamotoSatoshi en-aut-sei=Tsukamoto en-aut-mei=Satoshi kn-aut-name=塚本智史 kn-aut-sei=塚本 kn-aut-mei=智史 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=独立行政法人 放射線医学総合研究所・研究基盤センター・生物研究推進課 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=9 end-page=58 dt-received= dt-revised= dt-accepted= dt-pub-year=1961 dt-pub=196110 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental studies on the competition between crop plants and weeds. (2) kn-title=作物と雑草との競争に関する実験的研究(2) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KasaharaY. en-aut-sei=Kasahara en-aut-mei=Y. kn-aut-name=笠原安夫 kn-aut-sei=笠原 kn-aut-mei=安夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue= article-no= start-page=11 end-page=19 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=20140201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of potassium nutrition on fruit development and yield of substrate grown strawberry kn-title=養液栽培イチゴの生育・収量と果実発育に及ぼすカリウム栄養の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=　Effect of K nutrition on sugar and organic acid concentration in fruit and yield of strawberry cultivars (Fragaria ×ananassa Duch.; Nyoho, Toyonoka, Sachinoka, Asukarubi, Akihime, Tochiotome, and Sagahonoka) was investigated. Nutrient solution (NO3 8, NH4 1, P 1, K 4, Ca 2, Mg 1, SO4 1 ; mM) was modified to contain 0 to 4mM of K by replacing K with Ca and supplied from the beginning of flowering. K in the drainage decreased to a trace level 3 weeks after the beginning of treatment except for 4mM‒K solution. Absorption of NO3 apparently decreased in plants supplied 0mM‒K solution, but little difference was observed among the other 3 solutions. K concentration in petiole decreased linearly with decrease in K concentration in solutions, but there was little difference between the concentrations of leaflet of plants supplied with 4 and 2mM‒K solutions. No difference was observed in concentrations of sugars and organic acids in fruit in primary inflorescence. In the second inflorescence, organic acids and K concentration in fruit linearly decreased with decrease in K in supplied solutions while sugar concentration of fruit decreased significantly only in plants supplied 0mM‒K solution. When 0.5 to 4mM‒K solutions were supplied from 2 weeks after planting, marketable fruit yield was smallest in plants supplied 0.5mM‒K solution followed, by that supplied 1mM‒K solution, and largest in that supplied with 2mM‒K solution. Almost all K supplied with 2mM‒K solution was absorbed by strawberry plants and no difference was observed in quality and K concentration of fruit, yield and plant growth between the plants supplied with 2 and 4mM‒K solutions. It may be suitable to reduce the concentration of K in nutrient solution by half for substrate production of strawberry. en-copyright= kn-copyright= en-aut-name=YoshidaYuichi en-aut-sei=Yoshida en-aut-mei=Yuichi kn-aut-name=吉田裕一 kn-aut-sei=吉田 kn-aut-mei=裕一 aut-affil-num=1 ORCID= en-aut-name=OhmoriToshimasa en-aut-sei=Ohmori en-aut-mei=Toshimasa kn-aut-name=大森敏正 kn-aut-sei=大森 kn-aut-mei=敏正 aut-affil-num=2 ORCID= en-aut-name=GotoTanjuro en-aut-sei=Goto en-aut-mei=Tanjuro kn-aut-name=後藤丹十郎 kn-aut-sei=後藤 kn-aut-mei=丹十郎 aut-affil-num=3 ORCID= en-aut-name=TanakaYoshiyuki en-aut-sei=Tanaka en-aut-mei=Yoshiyuki kn-aut-name=田中義行 kn-aut-sei=田中 kn-aut-mei=義行 aut-affil-num=4 ORCID= en-aut-name=MurakamiKenji en-aut-sei=Murakami en-aut-mei=Kenji kn-aut-name=村上賢治 kn-aut-sei=村上 kn-aut-mei=賢治 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=農林水産省中国四国農政局 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=石川県立大学生物資源環境学部 en-keyword=Fragaria ×ananassa Duch. kn-keyword=Fragaria ×ananassa Duch. en-keyword=K/Ca ratio kn-keyword=K/Ca ratio en-keyword=organic acids kn-keyword=organic acids en-keyword=sugars kn-keyword=sugars END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=トマト果実の成熟機構に関する分子生物学的研究 －マクロアレイとVIGS法を用いた解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=閻瑞 kn-aut-sei=閻 kn-aut-mei=瑞 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=低悪性度末梢性T細胞リンパ腫の形態的・分子生物学的・臨床学的特徴 kn-title=Distinct morphologic, phenotypic, and clinical-course characteristics of indolent peripheral T-cell lymphoma en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=HayashiEiko en-aut-sei=Hayashi en-aut-mei=Eiko kn-aut-name=林詠子 kn-aut-sei=林 kn-aut-mei=詠子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=229 end-page=237 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Anionic surfactant concentration in water of Lake Kojima and influent rivers kn-title=児島湖および流入河川水中の陰イオン界面活性剤濃度 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= en-aut-name=AoyamaI. en-aut-sei=Aoyama en-aut-mei=I. kn-aut-name=青山勲 kn-aut-sei=青山 kn-aut-mei=勲 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=203 end-page=227 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characteristics of water quality fluctuation in Lake Kojima kn-title=児島湖における水質の変動特性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AoyamaI. en-aut-sei=Aoyama en-aut-mei=I. kn-aut-name=青山勲 kn-aut-sei=青山 kn-aut-mei=勲 aut-affil-num=1 ORCID= en-aut-name=NishizakiH. en-aut-sei=Nishizaki en-aut-mei=H. kn-aut-name=西崎日佐夫 kn-aut-sei=西崎 kn-aut-mei=日佐夫 aut-affil-num=2 ORCID= en-aut-name=UrakamiY. en-aut-sei=Urakami en-aut-mei=Y. kn-aut-name=浦上佳子 kn-aut-sei=浦上 kn-aut-mei=佳子 aut-affil-num=3 ORCID= en-aut-name=MoriiF. en-aut-sei=Morii en-aut-mei=F. kn-aut-name=森井ふじ kn-aut-sei=森井 kn-aut-mei=ふじ aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=189 end-page=201 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The mechanism of accumulation of manganese, iron and arsenic in the bottom sediment of Lake Biwa kn-title=琵琶湖湖底堆積物中のマンガン, 鉄, ヒ素の蓄積機構 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=NakashimaS. en-aut-sei=Nakashima en-aut-mei=S. kn-aut-name=中島進 kn-aut-sei=中島 kn-aut-mei=進 aut-affil-num=1 ORCID= en-aut-name=MoriiF. en-aut-sei=Morii en-aut-mei=F. kn-aut-name=森井ふじ kn-aut-sei=森井 kn-aut-mei=ふじ aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=171 end-page=188 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Chemical studies on the natural waters kn-title=陸水の化学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoriiF. en-aut-sei=Morii en-aut-mei=F. kn-aut-name=森井ふじ kn-aut-sei=森井 kn-aut-mei=ふじ aut-affil-num=1 ORCID= en-aut-name=KobayashiJ. en-aut-sei=Kobayashi en-aut-mei=J. kn-aut-name=小林純 kn-aut-sei=小林 kn-aut-mei=純 aut-affil-num=2 ORCID= en-aut-name=MiyakeS. en-aut-sei=Miyake en-aut-mei=S. kn-aut-name=三宅幸子 kn-aut-sei=三宅 kn-aut-mei=幸子 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=4 article-no= start-page=165 end-page=169 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820327 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Curriculum vitae of Professor F. MORII kn-title=森井ふじ博士略歴、森井ふじ博士研究論文および著書目録 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=大原農業研究所 kn-aut-sei=大原農業研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=149 end-page=164 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Distribution of phosphorus, arsenic, cadmium, lead, zinc, copper, manganese, and iron contained in the bottom sediments of several lakes and seas in Japan kn-title=海底および湖底堆積物中のリン, ヒ素, カドミウム, 鉛, 亜鉛, 銅, マンガンおよび鉄の分布について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoriiF. en-aut-sei=Morii en-aut-mei=F. kn-aut-name=森井ふじ kn-aut-sei=森井 kn-aut-mei=ふじ aut-affil-num=1 ORCID= en-aut-name=NakashimaS. en-aut-sei=Nakashima en-aut-mei=S. kn-aut-name=中島進 kn-aut-sei=中島 kn-aut-mei=進 aut-affil-num=2 ORCID= en-aut-name=NishizakiH. en-aut-sei=Nishizaki en-aut-mei=H. kn-aut-name=西崎日佐夫 kn-aut-sei=西崎 kn-aut-mei=日佐夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=141 end-page=148 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Variations of calcium, phosphorus, magnesium, and cadmium contents in anomalous-vertebrae fish induced by cadmium kn-title=カドミウムによって誘発された脊椎骨異常魚のカルシウム, 燐, マグネシウムおよびカドミウム含有量の変化 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuramotoS. en-aut-sei=Muramoto en-aut-mei=S. kn-aut-name=村本茂樹 kn-aut-sei=村本 kn-aut-mei=茂樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=125 end-page=139 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Fluctuation of water quality and growth characteristics of water hyacinth, Eichhornia crassipes (Mart.) Solms, in natural water area kn-title=自然水域における水質変動とホテイアオイEichhornia crassipes(Mart.) Solmsの生長特性 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AoyamaI. en-aut-sei=Aoyama en-aut-mei=I. kn-aut-name=青山勲 kn-aut-sei=青山 kn-aut-mei=勲 aut-affil-num=1 ORCID= en-aut-name=OkiY. en-aut-sei=Oki en-aut-mei=Y. kn-aut-name=沖陽子 kn-aut-sei=沖 kn-aut-mei=陽子 aut-affil-num=2 ORCID= en-aut-name=NishizakiH. en-aut-sei=Nishizaki en-aut-mei=H. kn-aut-name=西崎日佐夫 kn-aut-sei=西崎 kn-aut-mei=日佐夫 aut-affil-num=3 ORCID= en-aut-name=NakagawaK. en-aut-sei=Nakagawa en-aut-mei=K. kn-aut-name=中川恭二郎 kn-aut-sei=中川 kn-aut-mei=恭二郎 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= affil-num=4 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=3 article-no= start-page=113 end-page=124 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of two kinds of very early genes on yield and its components in barley kn-title=オオムギにおける2種の極早生遺伝子の収量とその構成要素に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YasudaS. en-aut-sei=Yasuda en-aut-mei=S. kn-aut-name=安田昭三 kn-aut-sei=安田 kn-aut-mei=昭三 aut-affil-num=1 ORCID= en-aut-name=HayashiJ. en-aut-sei=Hayashi en-aut-mei=J. kn-aut-name=林二郎 kn-aut-sei=林 kn-aut-mei=二郎 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=107 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Factors on the termination of adult diapause and reproductive development in Gastrophysa atrocyanea Mot. (Coleoptera: Chrysomelidae) kn-title=コガタルリハムシの休眠覚醒と生殖器官の発育に関する要因 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AshidaY. en-aut-sei=Ashida en-aut-mei=Y. kn-aut-name=芦田栄徳 kn-aut-sei=芦田 kn-aut-mei=栄徳 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=101 end-page=106 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Pathways of glycogen metabolism in larvae of the rice stem borer, Chilo suppressalis Walker kn-title=ニカメイガにおけるグリコーゲン代謝系路について en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TsumukiH. en-aut-sei=Tsumuki en-aut-mei=H. kn-aut-name=積木久明 kn-aut-sei=積木 kn-aut-mei=久明 aut-affil-num=1 ORCID= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=2 ORCID= en-aut-name=ShiragaT. en-aut-sei=Shiraga en-aut-mei=T. kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=93 end-page=100 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparative study of the pygidial defensive systems of adults of Carabidae (Coleoptera) kn-title=オサムシ類成虫の尾部防御分泌系の比較 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KanehisaK. en-aut-sei=Kanehisa en-aut-mei=K. kn-aut-name=兼久勝夫 kn-aut-sei=兼久 kn-aut-mei=勝夫 aut-affil-num=1 ORCID= en-aut-name=YamashitaT. en-aut-sei=Yamashita en-aut-mei=T. kn-aut-name=山下俊和 kn-aut-sei=山下 kn-aut-mei=俊和 aut-affil-num=2 ORCID= en-aut-name=ShiragaT. en-aut-sei=Shiraga en-aut-mei=T. kn-aut-name=白神孝 kn-aut-sei=白神 kn-aut-mei=孝 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=87 end-page=92 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of pH and sugars on the development of the cowpea aphid, Aphis craccivora Koch, fed on synthetic diet kn-title=人工飼育マメアブラムシAphis craccivora Kochの発育に及ぼすpHと糖の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和維 kn-aut-sei=河田 kn-aut-mei=和維 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=81 end-page=86 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influence of temperature on the development of Telmatoscopus albipunctatus Williston kn-title=オオチョウバエの発育に及ぼす温度の影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MoriharaO. en-aut-sei=Morihara en-aut-mei=O. kn-aut-name=森原修 kn-aut-sei=森原 kn-aut-mei=修 aut-affil-num=1 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=73 end-page=79 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Occurrence of sexual forms of the green peach aphid, Myzus persicae Sulz. in Kurashiki district kn-title=倉敷地方におけるモモアカアブラムシMyzus persicae Sulz.の両性型出現 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=MuraiT. en-aut-sei=Murai en-aut-mei=T. kn-aut-name=村井保 kn-aut-sei=村井 kn-aut-mei=保 aut-affil-num=1 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=63 end-page=72 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Habitat segregation of the Sitophilus Complex (Coleoptera: Curculionidae), with special reference to the reduction of body size kn-title=コクゾウ類Sitophilus Complexの小型化と棲み分けについて en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ObaraK. en-aut-sei=Obara en-aut-mei=K. kn-aut-name=尾原和夫 kn-aut-sei=尾原 kn-aut-mei=和夫 aut-affil-num=1 ORCID= en-aut-name=YasueY. en-aut-sei=Yasue en-aut-mei=Y. kn-aut-name=安江安宣 kn-aut-sei=安江 kn-aut-mei=安宣 aut-affil-num=2 ORCID= en-aut-name=KawadaK. en-aut-sei=Kawada en-aut-mei=K. kn-aut-name=河田和雄 kn-aut-sei=河田 kn-aut-mei=和雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=2 article-no= start-page=57 end-page=61 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810316 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Curriculum vitae of Emeritus Professor Y. YASUE kn-title=安江安宜博士略歴、安江安宣博士研究論文および著書目録 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=大原農業研究所 kn-aut-sei=大原農業研究所 kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=59 cd-vols= no-issue=1 article-no= start-page=37 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=19810225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Multiple forms of α-glucosidase with different substrate specificities from plant seeds kn-title=種々の種子からの基質特異性が異なる多型のα-グルコシダーゼ en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SuzukiY. en-aut-sei=Suzuki en-aut-mei=Y. kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=1 ORCID= en-aut-name=YamasakiY. en-aut-sei=Yamasaki en-aut-mei=Y. kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=UchidaK. en-aut-sei=Uchida en-aut-mei=K. kn-aut-name=内田絅 kn-aut-sei=内田 kn-aut-mei=絅 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END