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Author Wakabayashi, Takanobu|
Published Date 1990-12
Publication Title 岡山医学会雑誌
Volume volume102
Issue issue11-12
Content Type Journal Article
Author Kunishio, Katsuzo|
Published Date 1990-06
Publication Title 岡山医学会雑誌
Volume volume102
Issue issue5-6
Content Type Journal Article
Author Kikumoto, Yoko|
Published Date 2010-06-30
Publication Title
Content Type Thesis or Dissertation
JaLCDOI 10.18926/21178
Title Alternative Determination of copper and zinc in synovial fluid by atomic absorption spectrophotometry
FullText URL pitsr_048_013_023.pdf
Author Furuno, Katsushi|
Abstract Copper (Cu) and zinc (Zn) in synovial fluids of 52 patients with rheumatoid arthritis (RA) and of 53 patients with osteoarthritis (OA) were determined using a HITACHI MODEL 207 atomic absorption spectrophotometer. Standard solution of Cu and Zn were 50μg, 100μg, 200μg and 300μg per 100 ml 20 v/v % glycerine containing 0.01 N-HCl. To 0.5ml synovial fluid, 50 units of hyaluronidase was added and then the mixture were incubated for 20 minutes at 37℃. Synovial fluid treated with hyaluronidase and standard solution of Cu and Zn were diluted five fold with deionized distillation water, then aspirated directly into the burner. The relative absorbance was expressed as scale reading by a HITACHI MODEL QPD-54 recoder. The mean recovery rate of Cu and Zn in synovial fluids were 102.0% and 101.9%. By this method the concentrations of Cu and Zn in 52 RA (12 males, 40 females) were 97.6±29.0 (S.D.) μg/100 ml and 83.9±40.1 (S.D.) μg/100 ml ; 97.3±27.1 (S.D.)μg/100 ml, 78.7±29.9 (S.D.) μg /100 ml in 12males and 97.7±29.5 (S.D.) μg/100 ml, 85.5±42.6 (S.D.) μg /100 ml in 40 females respectively. The concentrations of Cu and Zn in 53 OA (14 males, 39 females) were 46.9±14.5 (S.D.) μg/100 ml and 40.8±14.3 (S.D.) μg/100 ml; 47.3±9.7 (S.D.) JLg/100 ml, 45.1±19.0 (S.D.) μg/ 100 ml in 14 males and 46.8±15.8 (S.D.) μg/100 ml, 39.3±11.8 (S.D.) μg/100 ml in 39 females respectively. No sex differences of Cu and Zn values were observed in both RA and OA. In patients with RA, concentrations of Cu and Zn in synovial fluids were signifcantly higher than in OA in both sexes. There were positive correlation between the concentrations of Cu and Zn in 105 patients (RA and OA, r=0.74, p<0.01) , in 52 RA (r=0.54, p<0.01), in 53 OA (r=0.81, p<0.01). In patients with RA, there were no correlation between stage and concentrations of Cu and Zn in synovial fluids.
Publication Title 岡山大学温泉研究所報告
Published Date 1979-03-25
Volume volume48
Start Page 13
End Page 23
ISSN 0369-7142
language Japanese
File Version publisher
NAID 120002311026
Author Yamamoto, Toshio|
Published Date 1988-04
Publication Title 岡山実験動物研究会報
Volume volume6
Content Type Journal Article
Author Asada, Nobuhiko|
Published Date 1990-06
Publication Title 岡山実験動物研究会報
Volume volume7
Content Type Others
Author Kawata, Tetsunori| Yamada, Kazuhiro| Wada, Masahiro| Tadokoro, Tadahiro| Maekawa, Akio|
Published Date 1992-06
Publication Title 岡山実験動物研究会報
Volume volume9
Content Type Journal Article
Author Saigusa, Masayuki|
Published Date 1992-06
Publication Title 岡山実験動物研究会報
Volume volume9
Content Type Others
Author Iwado, Akimasa| Mukuno, Takashi| Akizawa, Hiromichi| Mifune, Masaki| Saito, Yutaka|
Published Date 1998-08
Publication Title 環境制御
Volume volume20
Content Type Departmental Bulletin Paper
Author 岡山大学農学部|
Published Date 2010-02-01
Publication Title 岡山大学農学部学術報告
Volume volume99
Issue issue1
Content Type Others
Author Tanaka, Takao|
Published Date 1958-12-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue12
Content Type Journal Article
Author Tanaka, Takao|
Published Date 1958-12-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue12
Content Type Journal Article
Author Ono, Yasuzo|
Published Date 1958-11-30
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue11
Content Type Journal Article
Author Fukuda, Masao|
Published Date 1958-10-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue10
Content Type Journal Article
Author Fukuda, Masao|
Published Date 1958-10-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue10
Content Type Journal Article
Author Fukuda, Masao|
Published Date 1958-10-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue10
Content Type Journal Article
Author Izumihara, Mitsue|
Published Date 1959-02-28
Publication Title 岡山医学会雑誌
Volume volume71
Issue issue2-2
Content Type Journal Article
Author Nishiwaki, Korenobu|
Published Date 1958-07-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue7
Content Type Journal Article
Author Inoue, Masakatsu|
Published Date 1958-07-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue7
Content Type Journal Article
Author Shiomi, Fumitoshi|
Published Date 1958-07-31
Publication Title 岡山医学会雑誌
Volume volume70
Issue issue7
Content Type Journal Article