result 47755 件
| JaLCDOI | 10.18926/AMO/49253 |
|---|---|
| FullText URL | 67_1_19.pdf |
| Author | Furukawa, Masashi| Soh, Junichi| Yamamoto, Hiromasa| Ichimura, Kouichi| Shien, Kazuhiko| Maki, Yuho| Muraoka, Takayuki| Tanaka, Norimitsu| Ueno, Tsuyoshi| Asano, Hiroaki| Tsukuda, Kazunori| Toyooka, Shinichi| Miyoshi, Shinichiro| |
| Abstract | Nuclear factor of κ-light polypeptide gene enhancer in B cells inhibitor α (NFKBIA), which is a tumor suppressor gene, was found to be silenced in lung adenocarcinomas. We examined NFKBIA expression, mutations in the EGFR and K-ras genes, and EML4-ALK fusion in 101 resected lung adenocarcinoma samples from never-smokers. NFKBIA expression was evaluated using immunohistochemistry. NFKBIA expression was negative in 16 of the 101 samples (15.8%). EGFR and K-ras mutations and EML4-ALK fusion were detected in 61 (60.5%), 1 (1.0%), and 2 (2.0%) of the 101 samples, respectively, in a completely mutually exclusive manner. Negative NFKBIA expression was observed significantly more frequently among the tumors with none of the three genetic alterations compared to those with such alterations (p＝0.009). In addition, negative NFKBIA expression was significantly more frequent among the EGFR-wild type samples compared to the EGFR-mutant samples (p＝0.013). In conclusion, NFKBIA expression was silenced in adenocarcinomas without EGFR/K-ras mutations or EML4-ALK fusion, suggesting that the silencing of NFKBIA may play an important role in the carcinogenesis of adenocarcinomas independent of EGFR/K-ras mutations or EML4-ALK fusion. |
| Keywords | never-smoker lung cancer adenocarcinoma nuclear factor of κ-light polypeptide gene enhancer in B cells inhibitor α epidermal growth factor receptor |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-02 |
| Volume | volume67 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 19 |
| End Page | 24 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23439505 |
| Web of Science KeyUT | 000316829900003 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/52534 |
| JaLCDOI | 10.18926/AMO/49252 |
|---|---|
| FullText URL | 67_1_9.pdf |
| Author | Fatmawati, Ni Nengah Dwi| Sakaguchi, Yoshihiko| Suzuki, Tomonori| Oda, Masataka| Shimizu, Kenta| Yamamoto, Yumiko| Sakurai, Jun| Matsushita, Osamu| Oguma, Keiji| |
| Abstract | Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs), the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival. |
| Keywords | botulinum phospholipase C botulinum toxin phospholipase C activity sphingomyelinase activity hemolytic activity |
| Amo Type | Original Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-02 |
| Volume | volume67 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 9 |
| End Page | 18 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23439504 |
| Web of Science KeyUT | 000316829900002 |
| Related Url | http://ousar.lib.okayama-u.ac.jp/metadata/49731 |
| JaLCDOI | 10.18926/AMO/49251 |
|---|---|
| FullText URL | 67_1_1.pdf |
| Author | Nishimori, Hisakazu| Maeda, Yoshinobu| Tanimoto, Mitsune| |
| Abstract | Graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation. Chronic GVHD often presents with clinical manifestations that resemble those observed in autoimmune diseases. Standard treatment is 1-2mg/kg/day of prednisone or an equivalent dose of methylprednisolone, with continued administration of a calcineurin inhibitor for steroid sparing. However, the prognosis of steroid-refractory chronic GVHD remains poor. Classically, chronic GVHD was said to involve predominantly Th2 responses. We are now faced with a more complex picture, involving possible roles for thymic dysfunction, transforming growth factor-β (TGF-β) and platelet-derived growth factor (PDGF), B cells and autoantibodies, and Th1/Th2/Th17 cytokines, as well as regulatory T cells (Tregs), in chronic GVHD. More detailed research on the pathophysiology of chronic GVHD may facilitate the establishment of novel strategies for its prevention and treatment. |
| Keywords | chronic GVHD Th17 Am80 regulatory T cell (Treg) steroid-refractory |
| Amo Type | Review |
| Publication Title | Acta Medica Okayama |
| Published Date | 2013-02 |
| Volume | volume67 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 1 |
| End Page | 8 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| Copyright Holders | CopyrightⒸ 2013 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 23439503 |
| Web of Science KeyUT | 000316829900001 |
| Author | 近藤 萬太郞| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 荒川 左千代| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 岡村 保| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 西門 義一| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 板野 新夫| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 山口 彌輔| |
|---|---|
| Published Date | 1926-07-10 |
| Publication Title | 農学研究 |
| Volume | volume9 |
| Content Type | Departmental Bulletin Paper |
| Author | 西門 義一| 三宅 忠一| |
|---|---|
| Published Date | 1926-03-01 |
| Publication Title | 農学研究 |
| Volume | volume8 |
| Content Type | Departmental Bulletin Paper |
| Author | 笠井 幹夫| |
|---|---|
| Published Date | 1926-03-01 |
| Publication Title | 農学研究 |
| Volume | volume8 |
| Content Type | Departmental Bulletin Paper |
| Author | 春川 忠吉| |
|---|---|
| Published Date | 1926-03-01 |
| Publication Title | 農学研究 |
| Volume | volume8 |
| Content Type | Departmental Bulletin Paper |
| Author | 笠井 幹夫| |
|---|---|
| Published Date | 1926-03-01 |
| Publication Title | 農学研究 |
| Volume | volume8 |
| Content Type | Departmental Bulletin Paper |
| Author | 近藤 萬太郎| |
|---|---|
| Published Date | 1926-03-01 |
| Publication Title | 農学研究 |
| Volume | volume8 |
| Content Type | Departmental Bulletin Paper |
| Author | 西門 義一| |
|---|---|
| Published Date | 1925-03-05 |
| Publication Title | 農学研究 |
| Volume | volume7 |
| Content Type | Departmental Bulletin Paper |
| Author | 春川 忠吉| |
|---|---|
| Published Date | 1925-03-05 |
| Publication Title | 農学研究 |
| Volume | volume7 |
| Content Type | Departmental Bulletin Paper |
| Author | 笠井 幹夫| |
|---|---|
| Published Date | 1925-03-05 |
| Publication Title | 農学研究 |
| Volume | volume7 |
| Content Type | Departmental Bulletin Paper |
| Author | 近藤 萬太郞| |
|---|---|
| Published Date | 1925-03-05 |
| Publication Title | 農学研究 |
| Volume | volume7 |
| Content Type | Departmental Bulletin Paper |
| Author | 宗定 哲二| |
|---|---|
| Published Date | 1924-07-07 |
| Publication Title | 農学研究 |
| Volume | volume6 |
| Content Type | Departmental Bulletin Paper |
| Author | 笠井 幹夫| |
|---|---|
| Published Date | 1924-07-07 |
| Publication Title | 農学研究 |
| Volume | volume6 |
| Content Type | Departmental Bulletin Paper |
