result 559 件
Title Alternative | Sexuality in plants, unveiled from genome evolution |
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FullText URL | srfa_109_037_040.pdf |
Author | Akagi, Takashi| |
Abstract | Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems. |
Keywords | Sex determination Sex chromosome Polyploidization Genome evolution Tree crops |
Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
Published Date | 2020-02-01 |
Volume | volume109 |
Start Page | 37 |
End Page | 40 |
ISSN | 2186-7755 |
language | Japanese |
File Version | publisher |
Title Alternative | Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells |
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FullText URL | srfa_109_007_011.pdf |
Author | Saeki, Nozomu| Moriya, Hisao| |
Abstract | Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency (fitness) produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy. |
Keywords | yeast 2 μ plasmid mathematical model |
Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
Published Date | 2020-02-01 |
Volume | volume109 |
Start Page | 7 |
End Page | 11 |
ISSN | 2186-7755 |
language | Japanese |
File Version | publisher |
FullText URL | JE36_161.pdf JE37_135.pdf |
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Author | Matsumura, Kentarou| Miyatake, Takahisa| |
Keywords | Artificial selection Quantitative trait Relaxation of selection Reverse selection Tonic immobility Tribolium castaneum |
Note | Matsumura, K., Miyatake, T. Correction to: Responses to relaxed and reverse selection in strains artificially selected for duration of death‑feigning behavior in the red flour beetle, Tribolium castaneum. J Ethol 37, 135 (2019) doi:10.1007/s10164-018-0579-3| |
Published Date | 2018-04-04 |
Publication Title | Journal of Ethology |
Volume | volume36 |
Issue | issue2 |
Publisher | Springer |
Start Page | 161 |
End Page | 168 |
ISSN | 0289-0771 |
NCID | AA10754455 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
DOI | 10.1007/s10164-018-0548-x |
Web of Science KeyUT | 000431173000007 |
Related Url | isVersionOf https://doi.org/10.1007/s10164-018-0548-x isVersionOf https://doi.org/10.1007/s10164-018-0579-3 |
FullText URL | GCA252_107.pdf |
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Author | Kunihiro, Takuya| Ota, Tsutomu| Nakamura, Eizo| |
Keywords | Lithium Oxygen Chondrule Chondrite Asteroid Allende Igneous rim SIMS |
Published Date | 2019-05-01 |
Publication Title | Geochimica et Cosmochimica Acta |
Volume | volume252 |
Publisher | Elsevier |
Start Page | 107 |
End Page | 125 |
ISSN | 0016-7037 |
NCID | AA00655038 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | author |
DOI | 10.1016/j.gca.2019.02.038 |
Web of Science KeyUT | 000462774200006 |
Related Url | isVersionOf https://doi.org/10.1016/j.gca.2019.02.038 |
FullText URL | JGPP_79_5_307.pdf |
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Author | Tamada, Tetsuo| Kondo, Hideki| |
Keywords | Soil-borne viruses Benyvirus Furovirus Pecluvirus Pomovirus Bymovirus Vector transmission Plasmodiophorids Polymyxa Spongospora |
Published Date | 2013-05-28 |
Publication Title | Journal of General Plant Pathology |
Volume | volume79 |
Issue | issue5 |
Publisher | Springer |
Start Page | 307 |
End Page | 320 |
ISSN | 1345-2630 |
NCID | AA11449093 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | author |
DOI | 10.1007/s10327-013-0457-3 |
Web of Science KeyUT | 000323517200003 |
Related Url | isVersionOf https://doi.org/10.1007/s10327-013-0457-3 |
JaLCDOI | 10.18926/AMO/57370 |
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FullText URL | 73_5_403.pdf |
Author | Ando, Akemi| Mitsuhashi, Toshiharu| Honda, Mitsugi| Hanayama, Yoshihisa| Hasegawa, Kou| Obika, Mikako| Kataoka, Hitomi| Otsuka, Fumio| |
Abstract | Osteoporosis increases the risk of bone fractures. It is diagnosed based on an individual’s bone mineral density (BMD) or a fracture without trauma. BMD is usually measured by the dual energy X-ray absorptiometry (DXA) method. Here we investigated factors for the earliest possible prediction of decreased BMD by examining the relationships between patients’ BMD values and changes in the patients’ physical and laboratory values. We retrospectively reviewed the medical records of 149 patients who visited our department in 2014-2015 for a variety of reasons and underwent an area BMD examination by DXA. We analyzed the relationships between decreasing BMD and the patients’ gender, age, body mass index (BMI), medical background, hemoglobin, electrolytes, and thyroid function. Thirty-nine of the patients were diagnosed with osteoporosis based on their T-scores. An adjusted analysis showed that female gender, aging, and increased serum calcium level were significantly related to decreasing femoral BMD, whereas high BMI was associated with an increase in femoral BMD. Collectively the results indicate that for the early detection of low BMD, it is important for general-practice physicians to consider conducting a BMD checkup when treating female and elderly patients with a low BMI and/or elevated serum calcium level. |
Keywords | bone mineral density (BMD) body mass index (BMI) female gender hypercalcemia osteoporosis |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2019-10 |
Volume | volume73 |
Issue | issue5 |
Publisher | Okayama University Medical School |
Start Page | 403 |
End Page | 411 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2019 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 31649366 |
Web of Science KeyUT | 000491886600005 |
FullText URL | VirusRes_262_37.pdf FIG_S1-7_revised.pdf TableS1-3.pdf |
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Author | Kondo, Hideki| Chiba, Sotaro| Maruyama, Kazuyuki| Andika, Ida Bagus| Suzuki, Nobuhiro| |
Keywords | Bumblebee Endogenous viral element Evolution Insect Plant alpha-like virus Transcriptome shotgun assembly Whole genome shotgun assembly |
Published Date | 2019-03-31 |
Publication Title | Virus Research |
Volume | volume262 |
Publisher | Elsevier Science |
Start Page | 37 |
End Page | 47 |
ISSN | 01681702 |
NCID | AA10642076 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © 2017 Elsevier B.V. All rights reserved. |
File Version | author |
PubMed ID | 29169832 |
DOI | 10.1016/j.virusres.2017.11.020 |
Web of Science KeyUT | 000459523800007 |
Related Url | isVersionOf https://doi.org/10.1016/j.virusres.2017.11.020 |
FullText URL | NatCom_10_1_3022.pdf |
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Author | Belay, Iyasu Getachew| Tanaka, Ryoji| Kitagawa, Hiroshi| Kobayashi, Katsura| Nakamura, Eizo| |
Published Date | 2019-07 |
Publication Title | Nature Communications |
Volume | volume10 |
Issue | issue1 |
Publisher | Springer Nature Publishing AG |
Start Page | 3022 |
ISSN | 20411723 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | © The Author(s) 2019 |
File Version | publisher |
PubMed ID | 31289264 |
DOI | 10.1038/s41467-019-10832-7 |
Web of Science KeyUT | 000474506700008 |
Related Url | isVersionOf https://doi.org/10.1038/s41467-019-10832-7 |
FullText URL | InfectGenetEvol_54_47.pdf |
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Author | Ghosh, Priyanka| Kumar, Dhirendra| Chowdhury, Goutam| Singh, Puneeta| Samanta, Prosenjit| Dutta, Shanta| Ramamurthy, T.| Sharma, N. C.| Sinha, Preety| Prasad, Yogendra| Shinoda, Sumio| Mukhopadhyay, Asish K.| |
Keywords | Cholera Vibrio cholerae ctxAB promoter ctxB gyrA rstB rtxA tcpA |
Published Date | 2017-10 |
Publication Title | Infection, Genetics and Evolution |
Volume | volume54 |
Publisher | Elsevier Science |
Start Page | 47 |
End Page | 53 |
ISSN | 15671348 |
NCID | AA11697619 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | author |
PubMed ID | 28625543 |
DOI | 10.1016/j.meegid.2017.06.015 |
Web of Science KeyUT | 000411461400006 |
Related Url | isVersionOf https://doi.org/10.1016/j.meegid.2017.06.015 |
JaLCDOI | 10.18926/ESR/56694 |
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FullText URL | esr_025_031_038.pdf |
Author | Amano, Hideki| Suzuki, Shigeyuki| Sato, Masaru| Yanagida, Makoto| |
Abstract | The study area is situated in Japan Sea side margin of the Shakotan Peninsula, Hokkaido, Japan, where MIS5e Terrace is preserved along the cost. Numbers of tight drilling have done to establish new method for terrace analysis. Buried wave cut terrace and sea cliff (when the MIS5e Terrace was formed) are reconstructed by distribution of the terrace deposits and these bottom of unconformity planes. The precise site and altitude of former shoreline was also obtained. Altitudes of the former shoreline from the 7 sections are almost the same 22 to 27m in height. Previous data of the height of shoreline are obtained from the topographic MIS5e Terrace surface. The altitudes have variation from 30m to 60m. It suggests that the traditional method for the MIS5e terrace analysis had some errors in the study area. |
Keywords | MIS5e marine terrace terrace deposits tight drilling shoreline |
Publication Title | Okayama University Earth Science Report |
Published Date | 2018-12-27 |
Volume | volume25 |
Issue | issue1 |
Start Page | 31 |
End Page | 38 |
ISSN | 1340-7414 |
language | English |
Copyright Holders | © 2018 by Okayama University Earth Science Reports Editorial Committee All Rights Reserved |
File Version | publisher |
JaLCDOI | 10.18926/ESR/56692 |
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Title Alternative | Synoptic climatological analyses on the large day-to-day variation of air temperature around Germany in winter (Relating to the “seasonal feeling” in “Fasnacht”, the festival for driving the winter away) |
FullText URL | esr_025_007_017.pdf |
Author | Hamaki, Tatsuya| Kato, Kuranoshin| Otani, Kazuo| Kato, Haruko| Matsumoto, Kengo| |
Abstract | Synoptic climatological analyses associated with the large day-to-day variation of air temperature around Germany in winter were performed based mainly on the NCEP/NCAR reanalysis data for 2000/2001 to 2010/2011 winters, with an interest in the relationship to the “seasonal feeling” in “Fasnacht”, the festival for driving the winter away. The intermittent appearance of the extremely cold days with rather large day-to-day variation in winter as pointed out by Kato et al. (2017) for around Germany was seen also to the north and to the east of Germany. According to a case study for the 2000/2001 winter, such large daily mean temperature fluctuation was closely related to the intraseasonal variation of the Icelandic low with about one month period, i.e., while the temperature around Germany was relatively higher at the eastward shift phase of the Icelandic low due to the strong warm air advection by the SW-ly wind, the temperature was extremely low there when the Icelandic low was weakened and retreated westward. |
Keywords | Comparative climatology Climate around Europe Synoptic climatology on daily temperature variation in winter around Germany seasonal cycle and “seasonal feeling” |
Publication Title | Okayama University Earth Science Report |
Published Date | 2018-12-27 |
Volume | volume25 |
Issue | issue1 |
Start Page | 7 |
End Page | 17 |
ISSN | 1340-7414 |
language | Japanese |
Copyright Holders | © 2018 by Okayama University Earth Science Reports Editorial Committee All Rights Reserved |
File Version | publisher |
FullText URL | Vaccine_32_A20-8.pdf |
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Author | Mullick, Satarupa| Mandal, Paulami| Mukti Kant Nayak| Ghosh, Souvik| De, Papiya| Rajendran, K.| Bhattacharya, Mihir K.| Mitra, Utpala| Ramamurthy, Thandavarayan| Kobayashi, Nobumichi| Chawla-Sarkar, Mamta| |
Keywords | Diarrhea Rotavirus India Kolkata G9 strains G2 strains |
Published Date | 2014-08-11 |
Publication Title | Vaccine |
Volume | volume32 |
Issue | issuesupplment 1 |
Publisher | Elsevier Science |
Start Page | A20 |
End Page | A28 |
ISSN | 0264410X |
NCID | AA10491877 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
PubMed ID | 25091674 |
DOI | 10.1016/j.vaccine.2014.03.018 |
Web of Science KeyUT | 000340977500007 |
Related Url | isVersionOf https://doi.org/10.1016/j.vaccine.2014.03.018 |
FullText URL | Front_Microbiol_7_1250.pdf |
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Author | Ghosh, Raikamal| Sharma, Naresh C.| Halder, Kalpataru| Bhadra, Rupak K.| Chowdhury, Goutam| Pazhani, Gururaja P.| Shinoda, Sumio| Mukhopadhyay, Asish K.| Nair, G. Balakrish| Ramamurthy, Thadavarayan| |
Keywords | V.cholerae O139 ribotypes CT genotype CTX prophage PFGE |
Published Date | 2016-08-09 |
Publication Title | frontiers in Microbiology |
Volume | volume7 |
Publisher | Frontiers Media S.A. |
Start Page | 1250 |
ISSN | 1664302X |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
PubMed ID | 27555841 |
DOI | 10.3389/fmicb.2016.01250 |
Web of Science KeyUT | 000381079700001 |
Related Url | isVersionOf https://doi.org/10.3389/fmicb.2016.01250 |
FullText URL | Plos_Negl_Trop_Dis_5386.pdf |
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Author | Imamura, Daisuke| Morita, Masatomo| Sekizuka, Tsuyoshi| Mizuno, Tamaki| Takemura, Taichiro| Yamashiro, Tetsu| Chowdhury, Goutam| Pazhani, Gururaja P.| Mukhopadhyay, Asish K.| Ramamurthy, Thandavarayan| Miyoshi, Shin-ichi| Kuroda, Makoto| Shinoda, Sumio| Ohnishi, Makoto| |
Note | This research is supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) from Ministry of Education, Culture, Sport, Science & Technology in Japan, and Japan Agency for Medical Research and Development (AMED).| |
Published Date | 2017-02-13 |
Publication Title | PLOS Neglected Tropical Diseases |
Volume | volume11 |
Issue | issue2 |
Publisher | PLOS |
Start Page | e0005386 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
PubMed ID | 28192431 |
DOI | 10.1371/journal.pntd.0005386 |
Web of Science KeyUT | 000396406600020 |
Related Url | isVersionOf https://doi.org/10.1371/journal.pntd.0005386 |
FullText URL | Geochem_Cosmochi_Act_235_76.pdf |
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Author | Inoue, Mayuri| Nakamura, Takashi| Tanaka, Yasuaki| Suzuki, Atsushi| Yokoyama, Yusuke| Kawahata, Hodaka| Sakai, Kazuhiko| Gussone, Nikolaus| |
Keywords | Coral symbiosis calcification pH geochemical tracers |
Note | This is an Accepted Manuscript of an article published by Elsevier B.V.| |
Published Date | 2018-08-15 |
Publication Title | Geochimica et Cosmochimica Acta |
Volume | volume235 |
Publisher | Elsevier B.V. |
Start Page | 76 |
End Page | 88 |
ISSN | 00167037 |
NCID | AA00655038 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | author |
DOI | 10.1016/j.gca.2018.05.016 |
Related Url | isVersionOf https://doi.org/10.1016/j.gca.2018.05.016 |
FullText URL | PhysRevB_97_094525.pdf |
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Author | Terashima, K.| Paris, E.| Simonelli, L.| Salas-Colera, E.| Puri, A.| Wakita, T.| Yamada, Y.| Nakano, S.| Idei, H.| Kudo, K.| Nohara, M.| Muraoka, Y.| Mizokawa, T.| Yokoya, T.| Saini, N. L.| |
Note | This is an article published by American Physical Society| |
Published Date | 2018-09-28 |
Publication Title | Physical Review B |
Volume | volume98 |
Issue | issue9 |
Publisher | American Physical Society |
Start Page | 094525 |
ISSN | 24699950 |
NCID | AA11187113 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
File Version | publisher |
DOI | 10.1103/PhysRevB.98.094525 |
Web of Science KeyUT | 000446178800008 |
Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.98.094525 |
JaLCDOI | 10.18926/AMO/56182 |
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FullText URL | 72_4_423.pdf |
Author | Sakakida, Kourin| Wei, Fan-Yan| Senokuchi, Takafumi| Shimoda, Seiya| Kakuma, Tatsuyuki| Araki, Eiichi| Tomizawa, Kazuhito| The Eperisone for Diabetes with Impaired tRNA (EDIT) Study Group| |
Abstract | Genetic variation in Cdk5 Regulatory Associated Protein 1-Like 1 (CDKAL1) is associated with the development of type 2 diabetes (T2D). Dysfunction of CDKAL1 impairs the translation of proinsulin, which leads to glucose intolerance. Eperisone, an antispasmodic agent, has been shown to ameliorate glucose intolerance in Cdkal1-deficient mice. We have launched a phase II clinical study to investigate the potential anti-diabetic effect of eperisone in T2D patients carrying risk or non-risk alleles of CDKAL1. The primary endpoint is the change of hemoglobin A1c (HbA1c) levels. We also examined whether the efficacy of eperisone in T2D patients is associated with CDKAL1 activity. |
Keywords | diabetes insulin secretion single nucleotide polymorphism glucose |
Amo Type | Clinical Study Protocol |
Publication Title | Acta Medica Okayama |
Published Date | 2018-08 |
Volume | volume72 |
Issue | issue4 |
Publisher | Okayama University Medical School |
Start Page | 423 |
End Page | 426 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2018 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 30140092 |
JaLCDOI | 10.18926/AMO/56073 |
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FullText URL | 72_3_275.pdf |
Author | Morizane, Shin| Ouchida, Mamoru| Sunagawa, Ko| Sugimoto, Saeko| Kobashi, Mina| Sugihara, Satoru| Nomura, Hayato| Tsuji, Kazuhide| Sato, Atsushi| Miura, Yoshihiro| Hattori, Hiroaki| Tada, Kotaro| Huh, Wook-Kang| Seno, Akemi| Iwatsuki, Keiji| |
Abstract | Lympho-epithelial Kazal-type-related inhibitor (LEKTI) is a large multidomain serine protease inhibitor that is expressed in epidermal keratinocytes. Nonsense mutations of the SPINK5 gene, which codes for LEKTI, cause Netherton syndrome, which is characterized by hair abnormality, ichthyosis, and atopy. A single nucleotide polymorphism (SNP) of SPINK5, p.K420E, is reported to be associated with the pathogenesis of atopic dermatitis (AD). We studied all 34 exons of the SPINK5 gene in Japanese 57 AD patients and 50 normal healthy controls. We detected nine nonsynonymous variants, including p.K420E; these variants had already been registered in the SNP database. Among them, p.R654H (n=1) was found as a heterozygous mutation in the AD patients, but not in the control. No new mutation was detected. We next compared the data of the AD patients with data from the Human Genetic Variation Database provided by Kyoto University; a significant difference was found in the frequency of the p.S368N genotype distribution. PolyPhen-2 and SIFT, two algorithms for predicting the functional effects of amino acid substitutions, showed significant scores for p.R654H. Therefore, R654H might be a risk factor for epidermal barrier dysfunction in some Japanese AD patients. |
Keywords | atopic dermatitis SPINK5 LEKTI serine protease inhibitor epidermal barrier dysfunction |
Amo Type | Original Article |
Publication Title | Acta Medica Okayama |
Published Date | 2018-06 |
Volume | volume72 |
Issue | issue3 |
Publisher | Okayama University Medical School |
Start Page | 275 |
End Page | 282 |
ISSN | 0386-300X |
NCID | AA00508441 |
Content Type | Journal Article |
language | English |
Copyright Holders | CopyrightⒸ 2018 by Okayama University Medical School |
File Version | publisher |
Refereed | True |
PubMed ID | 29926005 |
FullText URL | PhysRevB_95_245310.pdf |
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Author | Uesugi, Eri| Miao, Xiao| Ota, Hiromi| Goto, Hidenori| Kubozono, Yoshihiro| |
Note | This is an article published by American Physical Society| |
Published Date | 2017-01 |
Publication Title | Physical Review B |
Volume | volume95 |
Issue | issue24 |
Publisher | American Physical Society |
Start Page | 245310 |
ISSN | 2469-9950 |
NCID | AA11187113 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | American Physical Society |
File Version | publisher |
DOI | 10.1103/PhysRevB.95.245310 |
Web of Science KeyUT | 000403355300005 |
Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.95.245310 |
FullText URL | PhysRevB_95_064512.pdf |
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Author | Ichioka, Masanori| Kogan, V. G.| Schmalian, J.| |
Note | This is an article published by American Physical Society| |
Published Date | 2017-02 |
Publication Title | Physical Review B |
Volume | volume95 |
Issue | issue6 |
Publisher | American Physical Society |
Start Page | 064512 |
ISSN | 2469-9950 |
NCID | AA11187113 |
Content Type | Journal Article |
language | English |
OAI-PMH Set | 岡山大学 |
Copyright Holders | ©2017 American Physical Society |
File Version | publisher |
DOI | 10.1103/PhysRevB.95.064512 |
Web of Science KeyUT | 000394657300012 |
Related Url | isVersionOf https://doi.org/10.1103/PhysRevB.95.064512 |