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ID 19093
Eprint ID
19093
FullText URL
71_433.pdf 3.37 MB
Title Alternative
Enzymatic Properties of Sal. typhi (Ⅱ) Part 1. The Relationship between the Accelerative Action of Metal Ions and the Time of Addition in Oxygen-Uptake by Resting Cells Part 2. The Relationship of the Mutual Actions of Two Substrates with the Time of Addition in Oxygen Consumption
Author
Matsumoto, Masayoshi
Abstract
In the investigation of the relationship between the accelerative action of Mg(++) and Fe(++) on the O(2)-uptake of Sal. typhi and the time when such metal ions are added, the author obtained the following results: 1. The substrates that accelerate the oxygen consumption of cells in the presence of Mg(++) or Fe(++) are glucose, lactate, pvruvate, and acetate. 2. When metal ion is added prior to a substrate and the substrate is added 20 minutes, 40 minutes, 60 minuter or 120 minutes later, the O(2)-uptake curve makes an upward curvature along with the lapse of time. 3. In the case where a substrate is added before metal ion, the accelerative action on O(2) uptake is weakened in proportion to the length of delay in the metal ion addition. By studying the relationship of the mutual actions of two substrates variously coupled from several substances with the time of adding such a combination of substrates in O(2)-consumption of Sat. typhi, the author obtained the following results: 1. It has been found that there are three sets of cases; namely when two substrates are added simultaneously, the amount of O(2)-uptake is greater than the total O(2)-uptake of two individual substrates (acceleration); O(2)-uptake is about the same as the total of two substrates measured separately; and the amount of O(2)-uptake becomes less than the O(2)-uptake of single one of the two substrates (inhibition). 2. Looking over the relationship of the adding time of two substrates as to accelerate O(2)-uptake and the accelerative effect, there are two different sets; namely, the one where one substrate is added later than the other, the accelerative effect is greater; and on the contrary the other where such an effect is diminished. This phenomenon seems to be due to the gradings in the oxidation (combining with oxygen) of each substrate by cells.
Published Date
1959-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue2-1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
433
End Page
446
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/2-1/71_2-1_433/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
Japanese
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma