| ID | 64148 |
| FullText URL | |
| Author |
Fujisawa, Yuki
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ming, Lu
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Yamada, Daisuke
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takao, Tomoka
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takarada, Takeshi
Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
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| Abstract | Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato(+) cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato(+) cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.
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| Keywords | Tendon
Human pluripotent stem cells
Tenocytes
Tendon regeneration
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| Published Date | 2022-11-12
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| Publication Title |
STEM Cell Research & Therapy
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| Volume | volume13
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| Issue | issue1
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| Publisher | BMC
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| Start Page | 515
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| ISSN | 1757-6512
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| Content Type |
Journal Article
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| language |
English
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| OAI-PMH Set |
岡山大学
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| Copyright Holders | © The Author(s) 2022.
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| File Version | publisher
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| PubMed ID | |
| DOI | |
| Web of Science KeyUT | |
| Related Url | isVersionOf https://doi.org/10.1186/s13287-022-03203-5
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| License | http://creativecommons.org/licenses/by/4.0/.
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| Funder Name |
Japan Society for the Promotion of Science
Japan Agency for Medical Research and Development
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| 助成番号 | 21H02643
18bm0704024h0001
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