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ID 30464
JaLCDOI
FullText URL
Author
Miyazaki, Masahiro
Bai, Liyan
Sato, Jiro
Abstract

To select a suitable medium for serum-free primary culture of adult rat hepatocytes, ten commercially-available synthetic media were compared for their ability to maintain the cells under serum-free and serum-supplemented conditions with special reference to attachment, survival and albumin secretion. It was found that Williams' medium E and DM-160 medium were the best among the ten media for maintaining hepatocytes under serum-free conditions in primary culture.

Keywords
adult rat hepatocytes
serum-free primary culture
maintenance
Williams' medium E
DM-160 medium
Amo Type
Article
Publication Title
Acta Medica Okayama
Published Date
1990-02
Volume
volume44
Issue
issue1
Publisher
Okayama University Medical School
Start Page
9
End Page
12
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
English
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT